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Search Results: 1 - 10 of 195084 matches for " Roberto Acízelo de Souza "
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JOAQUIM NORBERTO E SUA CONTRIBUI O à EDI O DE TEXTOS E à CRíTICA LITERáRIA
Roberto Acízelo de Souza
Fólio : Revista de Letras , 2009,
Abstract: In Joaquim Norberto’s broad and heterogeneous works the texts editions hold an outstanding position. Literary criticism, in its turn, appears as an activity derived from that one, as well as in a lower status in the whole of his production. As a critic, he follows mainly a biographic orientation, frequently trimming the reconstitution of the studied writers’ lives with episodes worthy of sentimental feuilletons.
Verdade da Metafísica: Descartes na Rota da Descoberta dos Fundamentos da Ciência
Roberto Lima de Souza
Princípios : Revista de Filosofia , 1996,
Abstract:
Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis
Cariello AJ,Bispo PJ,de Souza GF,Pignatari AC
Clinical Ophthalmology , 2012,
Abstract: Angelino Julio Cariello,1 Paulo José Martins Bispo,2 Gabriela Freitas Pereira de Souza,3 Antonio Carlos Campos Pignatari,2 Marcelo Ganzarolli de Oliveira,3 Ana Luisa Hofling-Lima11Department of Ophthalmology, 2Division of Infectious Diseases, Federal University of S o Paulo, 3Institute of Chemistry, University of Campinas, Campinas, S o Paulo, BrazilBackground: The purpose of this study was to evaluate the antimicrobial activity of two nitric oxide donors, ie, S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylcysteine (SNAC), against clinical isolates from patients with infectious keratitis.Methods: Reference broth microdilution assays were performed to determine the minimum inhibitory and bactericidal concentrations for GSNO and SNAC against four American Type Culture Collection strains and 52 clinical isolates from patients with infectious keratitis as follows: 14 (26.9%) Pseudomonas species; 13 (25.0%) coagulase-negative Staphylococci; 10 (19.2%) Staphylococcus aureus; nine (17.3%) Serratia marcescens; and six (11.5%) Enterobacter aerogenes. Sterility control and bacterial growth control were also performed.Results: SNAC showed lower minimum inhibitory and bactericidal concentrations than GSNO for all clinical isolates from patients with infectious keratitis. For Gram-positive bacteria, mean minimum inhibitory and bactericidal concentrations were 2.1 ± 1.3 and 8.6 ± 3.8 mM for SNAC and 4.6 ± 3.2 and 21.5 ± 12.5 mM for GSNO (P < 0.01). For Gram-negative bacteria, mean minimum inhibitory and bactericidal concentrations were 3.3 ± 1.4 and 6.1 ± 3.4 mM for SNAC and 12.4 ± 5.4 and 26.5 ± 10.1 mM for GSNO (P < 0.01). The minimum bactericidal to inhibitory concentration ratio was ≤8 in 100% of all isolates tested for SNAC and in 94.2% tested for GSNO.Conclusions: SNAC and GSNO had effective inhibitory and bactericidal effects against bacterial isolates from keratitis. SNAC showed greater antimicrobial activity than GSNO against all bacteria. Gram-positive bacteria were more susceptible to the inhibitory and bactericidal effects of the S-nitrosothiols.Keywords: antimicrobial activity, S-nitroso-N-acetylcysteine, S-nitrosoglutathione, nitric oxide donors, infectious keratitis
Late Pleistocene Climatic Changes in Central Brazil Indicated by Freshwater Sponges  [PDF]
Vanessa de Souza Machado, Cecilia Volkmer-Ribeiro, Roberto Iannuzzi
International Journal of Geosciences (IJG) , 2014, DOI: 10.4236/ijg.2014.58071
Abstract:

The Cemitério Palaeolake deposit in Catalao, Goiás State, Brazil, comprises a single, complete lacustrine sequence, dated from less than 27,500 ± 4000 yr B.P. to more than 51,780 ± 400 14C yr B.P. The unprecedented presence of spongillite and diatomite layers in this deposit was investigated using spicules of continental sponges as proxy data for environmental and climate reconstruction, which was relatively extensive for the ages examined. From the analysis of the sedimentary features and the associations of spicules preserved in the deposit, it was possible to reconstruct the evolution of the limnic system. Five developmental stages were thus identified, corresponding to the installation (lotic and erosion phase), establishment and development of the lake and its colmatation, the latter correlating to the Last Glacial Maximum (LGM). Moreover, based on the ecological requirements of the sponge species identified in the deposit, it was possible to infer the likely weather patterns related to respective phases of the lake. The presence of the sponge species Corvoheteromeyenia australis, presently recorded only from southern South America, indicated that polar incursions originating from the Antarctic were frequent during the last glacial period and might have played a notable role in what is now central Brazil. However, for a short time, drier and hotter weather conditions might have predominated, as suggested by the presence of the species Corvomeyenia thumi.

Immobilization of Lipase from Candida rugosa on Mesoporous MCM 41  [PDF]
Roberto Rodrigues de Souza, Renata D. M. Ferreira
Journal of Biosciences and Medicines (JBM) , 2014, DOI: 10.4236/jbm.2014.24011
Abstract:

The use of enzymatic route for production of biofuels is growing up due the mild reaction conditions that this method provides, as well as reducing SOx emission. To reduce costs, it’s necessary to immobilize the enzyme, making possible to use it continuously as biocatalyst. The aim of this work was to measure the influence of the mass of support and pH used for immobilization of commercial lipase from Candida rugosa acquired by Sigma laboratory. The immobilization method chosen was adsorption on mesoporous and hydrophobic support MCM 41, this has been treated with nitric acid 10% v/v to remove any organic residue. Then, 20 ml of enzymatic solution in phosphate buffer (pH 6.0, 7.0 and 8.0; 50 mM) and 1 g/L was placed under constant stirring with 0.30 and 0.45 g of support. Aliquots were taken from the reaction medium and analyzed by spectrophotometry at 10 minutes intervals. A volume of 0.2 ml of supernatant was put with 1.8 ml of substrate p-NFL at 0.18 g/L, and the absorbance at 410 nm was analyzed. In four cases there was a sharp reduction of supernatant’s activity at first 10 minutes, that ratifies the big affinity of the enzyme for the support and the negative influence of pH about the activity. Using the calibration curve, it was possible to calculate the final activity of each immobilization batch. This work suggests the occurrence of diffusional effects, which means that the enzyme mobility was restricted due the excessive amount of support, and then, it lost a part of accessibility to substrate, reflecting in not expressive activity values, and changing the state of ionization of the components of the system.

A NECESSIDADE DE AG NCIAS REGULADORAS SOB A PERSPECTIVA DA NOVA ECONOMIA INSTITUCIONAL [doi: 10.5329/RECADM.20060502003]
Roberto Tadeu de Souza Junior
Revista Eletr?nica de Ciência Administrativa - RECADM , 2006, DOI: 10.5329/253
Abstract:
O que é isto - decido conforme minha consciência? Decido conforme minha consciência?
Michel Roberto Oliveira de Souza
Revista Espa?o Acadêmico , 2010,
Abstract: Resenha: STRECK, Lênio Luiz. O que é isto – decido conforme minha consciência? Porto Alegre: Livraria do Advogado, 2010.
O direito brasileiro: a preven o de passivo ambiental e seus efeitos no MERCOSUL
Paulo Roberto Pereira de Souza
Scientia Iuris , 1997,
Abstract: The environment themes are acquiring more and more importance in the MERCOSUL. The work presents an analyzes of the possible environmental damage that may happen from the actions projected for the MERCOSUL in the coming years. It also suggests an environmental law unification and shows a worry with the need of the building of a law discussed with the society and able to regulate the development in a way that will not harm the planet and give the opportunity to a adequate protection of the environment. Os temas ambientais cada vez mais adquirem relevo no MERCOSUL. O trabalho apresenta uma análise de possível impactos ambientais no ambito do MERCOSUL e mostra a preocupa o co possíveis danos ambientais que poder o decorrer das a es projetadas para o MERCOSUL nos próximos anos. Sugere também a uniformiza o de leis ambientais e manifesta uma preocupa o com a necessidade da constru o de um direito comunitário capaz de regular o desenvolvimento de forma sustentável e propiciar uma adequada tutela ao meio ambiente.
A Tutorial on Principal Component Analysis with the Accord.NET Framework
César Roberto de Souza
Computer Science , 2012,
Abstract: This document aims to clarify frequent questions on using the Accord.NET Framework to perform statistical analyses. Here, we reproduce all steps of the famous Lindsay's Tutorial on Principal Component Analysis, in an attempt to give the reader a complete hands-on overview on the framework's basics while also discussing some of the results and sources of divergence between the results generated by Accord.NET and by other software packages.
Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis
Cariello AJ, Bispo PJ, de Souza GF, Pignatari AC, de Oliveira MG, Hofling-Lima AL
Clinical Ophthalmology , 2012, DOI: http://dx.doi.org/10.2147/OPTH.S34830
Abstract: actericidal effect of S-nitrosothiols against clinical isolates from keratitis Original Research (1006) Total Article Views Authors: Cariello AJ, Bispo PJ, de Souza GF, Pignatari AC, de Oliveira MG, Hofling-Lima AL Published Date November 2012 Volume 2012:6 Pages 1907 - 1914 DOI: http://dx.doi.org/10.2147/OPTH.S34830 Received: 08 June 2012 Accepted: 10 September 2012 Published: 20 November 2012 Angelino Julio Cariello,1 Paulo José Martins Bispo,2 Gabriela Freitas Pereira de Souza,3 Antonio Carlos Campos Pignatari,2 Marcelo Ganzarolli de Oliveira,3 Ana Luisa Hofling-Lima1 1Department of Ophthalmology, 2Division of Infectious Diseases, Federal University of S o Paulo, 3Institute of Chemistry, University of Campinas, Campinas, S o Paulo, Brazil Background: The purpose of this study was to evaluate the antimicrobial activity of two nitric oxide donors, ie, S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylcysteine (SNAC), against clinical isolates from patients with infectious keratitis. Methods: Reference broth microdilution assays were performed to determine the minimum inhibitory and bactericidal concentrations for GSNO and SNAC against four American Type Culture Collection strains and 52 clinical isolates from patients with infectious keratitis as follows: 14 (26.9%) Pseudomonas species; 13 (25.0%) coagulase-negative Staphylococci; 10 (19.2%) Staphylococcus aureus; nine (17.3%) Serratia marcescens; and six (11.5%) Enterobacter aerogenes. Sterility control and bacterial growth control were also performed. Results: SNAC showed lower minimum inhibitory and bactericidal concentrations than GSNO for all clinical isolates from patients with infectious keratitis. For Gram-positive bacteria, mean minimum inhibitory and bactericidal concentrations were 2.1 ± 1.3 and 8.6 ± 3.8 mM for SNAC and 4.6 ± 3.2 and 21.5 ± 12.5 mM for GSNO (P < 0.01). For Gram-negative bacteria, mean minimum inhibitory and bactericidal concentrations were 3.3 ± 1.4 and 6.1 ± 3.4 mM for SNAC and 12.4 ± 5.4 and 26.5 ± 10.1 mM for GSNO (P < 0.01). The minimum bactericidal to inhibitory concentration ratio was ≤8 in 100% of all isolates tested for SNAC and in 94.2% tested for GSNO. Conclusions: SNAC and GSNO had effective inhibitory and bactericidal effects against bacterial isolates from keratitis. SNAC showed greater antimicrobial activity than GSNO against all bacteria. Gram-positive bacteria were more susceptible to the inhibitory and bactericidal effects of the S-nitrosothiols.
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