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Search Results: 1 - 10 of 7719 matches for " Roberta Lemos Freire "
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Outbreaks of toxoplasmosis in human beings and animal/ Surtos de toxoplasmose em seres humanos e animais
Rafael André Ferreira Dias,Roberta Lemos Freire
Semina : Ciências Agrárias , 2005,
Abstract: Toxoplasmosis is one of the biggest wild world zoonosis and it can attack blood warmed animals from many species. Outbreaks of toxoplasmosis in humans and animals are not related frequently and it could be due to either weak or asymptomatic characteristics. So, there are difficulties in the clinical characterization of this pathology with laboratorial confirmation and posterior notification. This review emphasizes the informed outbreaks in humans and animals, its sources of infection and ways of transmission that vary in accordance with local habits and sanitary conditions. It still approaches the genetic characterization of Toxoplasma gondii strains and prevention methods. A toxoplasmose é uma zoonoses de distribui o mundial e acomete animais de sangue quente das mais variadas espécies. Os surtos de toxoplasmose em seres humanos e animais freqüentemente n o s o relatados. Isto ocorre, possivelmente, em fun o desta infec o caracterizar-se por sintomas ausentes ou brandos tanto em humanos quanto em animais. Desta maneira, existem dificuldades na caracteriza o clínica desta patologia com confirma o laboratorial e posterior notifica o. Esta revis o enfatiza os surtos de toxoplasmose notificados em humanos e animais, suas fontes de infec o e vias de transmiss o que variam de acordo com os hábitos locais e as condi es sanitárias de cada regi o. Aborda ainda a caracteriza o genética de cepas de Toxoplasma gondii e métodos de preven o.
Aspectos sociopolíticos da epidemia de toxoplasmose em Santa Isabel do Ivaí (PR)
Almeida,Márcio José de; Oliveira,Luzia Helena Herrmann de; Freire,Roberta Lemos; Navarro,Italmar Teodorico;
Ciência & Saúde Coletiva , 2011, DOI: 10.1590/S1413-81232011000700071
Abstract: in 2002, due to a toxoplasmosis epidemic santa isabel do ivaí, paraná state, was the focus of sanitary investigations. four hundred and twenty six individuals had serology suggestive of acute t. gondii infection (igm reactor), considered the largest outbreak of toxoplasmosis ever reported in the world. this research was meant to identify actions carried out by the sanitation and health services sector at that time, highlighting the political conflicts that took place during the process and identifying the measures taken by the sanitary authorities during and after the epidemic period. this is an interdisciplinary study aimed at understanding major problems of public health like this one. the investigation was based on the contents of documents press and institutional and interviews. according to official data, the epidemic was caused by the contamination of one of the water reservoirs that supply the city. this research showed that political and social factors, as the party instability and the level of political dependence of local society, were largely responsible for the occurrence of the epidemic and for the difficulties faced by the health agents during such crisis.
Detection of antibodies to Paracoccidioides brasiliensis in horses from northern Region of Paraná State / Detec o de anticorpos para Paracoccidioides brasiliensis em cavalos da regi o norte do Estado do Paraná
Andréia Corrêa Corte,Eiko Nakagawa Itano,Roberta Lemos Freire,Zoilo Pires de Camargo
Semina : Ciências Agrárias , 2009,
Abstract: Paracoccidioidomycosis, caused by the dimorphic fungus Paracoccidioides brasiliensis, is the most prevalent human systemic mycosis in Latin America. Paracoccidioidomycosis affects mainly male rural workers, causing granulomatous lesions in organs such as lungs, liver and spleen. The participation of other animal species in the fungus eco-epidemiology in not well understood. The aim of this study was to evaluate the infection by P. brasiliensis in horses from the Northern Region of Paraná State. The serum samples from 100 horses were assayed by ELISA and Immunodiffusion test, using gp43 and exoantigen as antigens, respectively. A seropositivity of 30% was observed by ELISA test, although all samples were negative by immunodiffusion test. These results suggest that horses can be indicator of fungus presence in the ambient. A paracoccidioidomicose, causada pelo fungo dimórfico Paracoccidioides brasiliensis, é a micose sistêmica de maior prevalência em humanos na América Latina. A paracoccidioidomicose afeta principalmente trabalhadores rurais do sexo masculino, provocando les es granulomatosas em órg os como pulm es, fígado e ba o. A participa o de outras espécies de animais na eco-epidemiologia do fungo n o é bem compreendida. Este trabalho teve como objetivo avaliar a infec o por P. brasiliensis em cavalos da Regi o Norte do Estado do Paraná. Foram analisadas 100 amostras de soros de cavalos por ELISA e por Imunodifus o em gel de agar, utilizando como antígenos a gp43 e o exoantígeno de P. brasiliensis, respectivamente. A soropositividade observada foi de 30% por ELISA enquanto que por imunodifus o todos os soros foram negativos. Os resultados obtidos sugerem que cavalos podem ser indicadores da presen a do fungo no meio ambiente.
Occurrence of antibodies to Toxoplasma gondii in sheep from the Londrina Region of the Paraná State, Brazil/ Ocorrência de anticorpos anti-Toxoplasma gondii em ovinos da regi o de Londrina no Estado do Paraná
Liza Ogawa,Italmar Teodorico Navarro,Roberta Lemos Freire,Rosangela Claret de Oliveira
Semina : Ciências Agrárias , 2003,
Abstract: Three hundred and thirty nine serum samples were taken from sheep from Londrina region of the Paraná state, Brazil. Samples were analyzed by indirect immunofluorescence assay (IFA) for the detection of anti-Toxoplasma gondii IgG antibodies. Titers higher or equal to 64 were considered positives. One hundred and eighty five (54,6%) of the 339 samples tested were seropositive, with titres ranging from 64 to 65.536. All farms had positives animals. There was a statistical significant difference between seroprevalence to T. gondii and age. No significant difference was observed in the association between seropreva Londrina region of the Paraná state, Brazil. Samples were analyzed by indirect immunofluorescence assay (IFA) for the detection of anti-Toxoplasma gondii IgG antibodies. Titers higher or equal to 64 were considered positives. One hundred and eighty five (54,6%) of the 339 samples tested were seropositive, with titres ranging from 64 to 65.536. All farms had positives animals. There was a statistical significant difference between seroprevalence to T. gondii and age. No significant difference was observed in the association between seroprevalence and sex. It was not possible to associated T. gondiiseroprevalenceand presence of housed or stray cats, because these animals were present in all farms. The present work showed that T. gondii has a wide distribution in sheep from this region. Amostras de 339 soros de ovinos da microrregi o de Londrina no Estado do Paraná, foram analisadas através da imunofluorescência indireta para pesquisa de anticorpos anti-Toxoplasma gondii (IFI-IgG), adotando-se como ponto de corte a dilui o 1:64. Foram observados 185 (54,6%) ovinos sororeagentes ao T. gondii, com títulos variando de 64 a 65.536. Todas as propriedades apresentaram ovinos sororeagentes. Animais com idade igual ou maior a dois anos, apresentaram um maior número de sororeagentes, e n o foi observada diferen a estatística significativa quanto ao sexo. N o foi possível associar a soropositividade para T. gondii com a presen a de gatos domiciliados e/ou errantes, uma vez que foi constatada a presen a desses animais em todas as propriedades. A partir dos dados obtidos neste estudo, conclui-se que a toxoplasmose está presente no rebanho ovino da microrregi o de Londrina no Estado do Paraná.
Comparison of the serologic tests of Indirect Immunofluorescence, Rapid Conglutination, Indirect ELISA and Competition ELISA for detection of antibodies against Anaplasma marginale in cattle sera from different enzootic areas/ Compara o dos testes sorológicos de Imunofluorescência Indireta, Conglutina o Rápida, ELISA indireto e ELISA por competi o para a detec o de anticorpos contra o Anaplasma marginale em soros de bovinos de diferentes áreas enzoóticas
Elizabete Regina Marangoni Marana,Amauri A. Alfieri,Gisele Maria de Andrade,Roberta Lemos Freire
Semina : Ciências Agrárias , 2006,
Abstract: The serological techniques Rapid Conglutination Test (RCT), Indirect ELISA (iELISA) and Indirect Immunofluorescent Assay (IFA), using the competition ELISA (cELISA) as gold test, were comparatively evaluated to detect antibodies against Anaplasma marginale. A total of 453 sera from vaccinated and non vaccinated cattle and, collected from enzootic stability and instability areas were tested. iELISA, IFA and TCR presented kappa index = 0.77 (substantial); 0.57 and 0.49 (moderate), sensibility of 90.6%; 90.2% and 73.7% and specificity of 86.6%; 62.8%, and 79.3%, respectively. Therefore, iELISA presented better specificity than IFA and RCT, and can be indicated for more detailed serological investigations, detection of persistently infected animals in cattle herds and for monitorating of vaccination programs. IFA and TCR can be used in prevalence studies and to monitor cattle movement between different geographical regions. Os testes sorológicos de Conglutina o Rápida (TCR) Imunofluorescência Indireta (IFI) e Imunoenzimáticos Indireto (iELISA) utilizando ELISA por competi o (cELISA), como padr o ouro, foram avaliados comparativamente para a detec o de anticorpos contra o Anaplasma marginale. Foram utilizadas 453 amostras de soros sangüíneos de bovinos vacinados e n o-vacinados e de áreas de estabilidade e instabilidade enzoótica. O iELISA, IFI e TCR apresentaram respectivamente, índice kappa=0,77 (substancial), 0,57 e 0,49 (moderado), sensibilidade de 90,6%, 90,2% e 73,7% e especificidade de 86,6%, 62,8%, e 79,3%. O iELISA apresentou o melhor desempenho e maior especificidade, podendo ser indicado na avalia o do perfil sorológico de rebanhos, na detec o de animais persistentemente infectados e de animais submetidos a programas de vacina o. As técnicas de IFI e TCR, mesmo apresentando desempenho inferior, podem ser recomendadas para a realiza o de inquéritos epidemiológicos e para o monitoramento de animais em transito entre as diferentes regi es geográficas.
Prevalence of Toxoplasma gondii infection among slaughtered swines in Paraná State, Brazil/ Prevalência da infec o por Toxoplasma gondii em suínos abatidos no Estado do Paraná, Brasil
Regina Takasawa Carletti,Roberta Lemos Freire,Marina Tie Shimada,Bruno Bergamo Ruffolo
Semina : Ciências Agrárias , 2005,
Abstract: The aim of this study was to verify toxoplasmosis’s prevalence in swine from slaughterhouses with inspection service in Paraná State. 13 of the 114 abattoirs were randomized in accordance with each State region and with the minimum daily abate of 30 animals. 424 swine blood samples were collected from October 2002 to January 2003. The Indirect Immunofluorescence Test (IFAT) was realized to detect Toxoplasma gondii antibodies (IgG) and 17 (4,00%) were positive, considering titers ? 64. According to the animals categories, six from 29 sows (20,69%) and 11 from 395 finisher pigs (2,60%) were positives. This difference was significant (p=0,0004) and could be related with the sow’s period of exposition to toxoplasmosis risk factors. Comparing this research to previous ones in Paraná State, we could see a tendency of decrease in the toxoplasmosis prevalence in pig farms, maybe related to the swine industry improvements in the last 15 years. Objetivou-se determinar a prevalência da toxoplasmose em suínos abatidos em frigoríficos com inspe o sanitária do Estado do Paraná. Do total de 114 abatedouros foram selecionados aleatoriamente 13 com média diária de abate de 30 ou mais animais, de acordo com cada mesorregi o paranaense. Coletou-se 424 amostras de sangue no período de outubro de 2002 a janeiro de 2003. Os soros foram submetidos à pesquisa de anticorpos anti-T. gondii (IgG) pela imunofluorescência indireta e 17 (4,00%) foram positivos, considerando títulos ? 64. De acordo com as categorias zootécnicas, seis das 29 matrizes avaliadas (20,69%) e 11 dos 395 animais de termina o (2,60%) foram positivos.Tal diferen a foi estatisticamente significativa (p=0,0004) e pode estar relacionada ao maior tempo de exposi o das matrizes aos fatores de risco associados à toxoplasmose. Comparando-se este estudo a outros previamente realizados no Estado do Paraná, observou-se uma tendência de decréscimo da prevalência da toxoplasmose no rebanho suíno, provavelmente devido às melhorias sanitárias adotadas nos últimos 15 anos.
Neospora caninum in dairy cattle in Paraná State, Brazil: histological and immunohistochemical analys is in fetuses/ Neospora caninum em vacas leiteiras no Estado do Paraná, Brasil: análise histológica e imuno-histoquímica em fetos
Ana Paula Millet Evangelista dos Santos,Italmar Teodorico Navarro,Roberta Lemos Freire,Odilon Vidotto
Semina : Ciências Agrárias , 2005,
Abstract: Thirty-four bovine abortions (fetuses) from Paraná State were examined histologically and immunohistochemically. Eight fetuses had non-suppurative encephalitis and miocarditis, and Neospora caninum were detected by immunohistochemistry. Four cows were seropositive to N. caninum. It was the first detection of congenital neosporosis in aborted bovines fetuses, in Paraná State, Brazil. Trinta e quatro fetos bovinos abortados, no Estado do Paraná, foram examinados através de análise histológica e imuno-histoquímica. O Neospora caninum foi detectado, pela imuno-histoquímica, em oito fetos que apresentavam miocardite e encefalite n o supurativa. Quatro vacas foram soropositivas para o Neospora caninum. Este é o primeiro relato de neosporose congênita em fetos bovinos abortados, no Estado do Paraná, Brasil.
Seroepidemiology of leptospirosis in pet dogs in the urban area of the municipality of Jataizinho, Paraná Soroepidemiologia da leptospirose em c es domiciliados na área urbana do município de Jataizinho, Paraná
Aline do Nascimento Benitez,Daniela Dib Gon?alves,Roberta Lemos Freire,Wagner Borges Rodrigues
Semina : Ciências Agrárias , 2012,
Abstract: The study of the prevalence of anti-leptospiral antibodies in pet dogs in urban areas and the variables associated with the risk of infection can aid in the identification of the serovars present in the region and indicated the reservoirs involved in this important zoonosis. The aim of the present study was to determine the prevalence of anti-leptospiral antibodies in 653 dogs from 369 households distributed throughout the urban area of the municipality of Jataizinho, Paraná (PR), and to investigate the variables associated with the risk of leptospiral infection in these animals. Serum samples were subjected to microscopic agglutination tests (MATs) against 23 serovars of pathogenic leptospires. Among the 132 (20.21%) reactive samples, 15 (11.36%) were reactive for more than one serovar, and the most likely serovar could not be determined. Among the 117 (88.73%) samples that reacted to a single serovar, the serovar Canicola was considered to be the most likely in 89 cases (76.07%). Variables associated with the risk of disease were investigated using an epidemiological questionnaire administered to each owner, and the data obtained were analyzed using the statistical software EpiInfo. The significant variables (p ? 0.05) associated with seropositivity for leptospirosis in pet dogs in the urban area of the municipality of Jataizinho, PR, were the presence of rats in the household, adult or elderly dogs, free access of the dog to the street and contact with stray dogs. The results indicate a high prevalence of pet dogs in the urban area of Jataizinho, PR, with anti-leptospiral antibodies and suggest the need to educate the population of the municipality about the vaccination of dogs, as well as, the epidemiological role of the dog in urban leptospirosis. O estudo da prevalência de c es domiciliados em áreas urbanas com anticorpos antileptospiras e de variáveis associadas ao risco de infec o, pode auxiliar na identifica o dos sorovares presentes na regi o e sugerir os reservatórios envolvidos nesta importante zoonose. O objetivo deste trabalho foi determinar a prevalência de anticorpos antileptospiras em 653 c es de 369 domicílios distribuídos na área urbana do município de Jataizinho, PR, e estudar variáveis associadas ao risco de infec o por leptospiras nesses animais. As amostras de soro foram submetida a prova de soroaglutina o microscópica (SAM) frente a 23 sorovares de leptospiras patogênicas. Das 132 (20,21%) amostras de c es reagentes, 15 (11,36%) reagiram para mais de um sorovar, n o sendo possível determinar o sorovar mais provável, e das 117
Toxoplasma gondii infection in pregnancy
Lopes, Fabiana Maria Ruiz;Gon?alves, Daniela Dib;Mitsuka-Breganó, Regina;Freire, Roberta Lemos;Navarro, Italmar Teodorico;
Brazilian Journal of Infectious Diseases , 2007, DOI: 10.1590/S1413-86702007000500011
Abstract: toxoplasmosis is caused by an intracellular protozoan, toxoplasma gondii, which has a wide geographical distribution. the main infection routes are ingestion of cysts from raw or badly-cooked meat, ingestion of oocysts from substrates contaminated with the feces of infected felines and congenital transmission by tachyzoites. the congenital form results in a severe systemic disease, because if the mother is infected for the first time during gestation, she can present a temporary parasitemia that will infect the fetus. many of the clinical symptoms are seen in congenitally-infected children, from a mild disease to serious signs, such as mental retardation. early diagnosis during the pregnancy is highly desirable, allowing prompt intervention in cases of infection, through treatment of pregnant women, reducing the probability of fetal infection and consequent substantial damage to the fetus. conventional tests for establishment of a fetal diagnosis of toxoplasmosis include options from serology to pcr. prevention of human toxoplasmosis is based on care to avoid infection, understanding the disease and serological exams during gestation. pregnant women should be tested serologically from three months gestation, until one month after childbirth. inclusion of serology for congenital toxoplasmosis along with the basic guthrie test for pku is of fundamental importance for early diagnosis of infection and so that treatment is initiated, in order to avoid possible sequels in the infant.
Soroprevalência de Anaplasma marginale em bovinos da regi?o Centro-Sul do estado do Paraná, Brasil, por um teste imunoenzimático competitivo utilizando proteína recombinante MSP5-PR1
Marana, Elizabete Regina Marangoni;Dias, Juliana Alves;Freire, Roberta Lemos;Vicentini, Josy Campanh?;Vidotto, Marilda Carlos;Vidotto, Odilon;
Revista Brasileira de Parasitologia Veterinária , 2009, DOI: 10.4322/rbpv.01801004
Abstract: anaplasma marginale prevalence was determined in 223 sera samples in 2-year old or older cattle, from the center- southern region of the paraná state, including ponta grossa, guarapuava and laranjeiras do sul municipalities. a survey of antibodies igg class against anaplasma marginale was performed through a competitive immune absorbent assay (celisa-pr1). from the 223 sera examined, 130 (58.74%) reacted to celisa-pr1 test, suggesting an region of enzootic instability, with a significant percentage of animals susceptible to infection by a. marginale and potentially in risk to develop anaplasmosis. the kind of exploration in the property, the breeding and handling system, the presence of other animals (ovine/caprine, horses, wild animals), and means of commercialization of animals were analyzed. the statistical analysis showed that there were no significant differences among the analyzed variables.
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