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Search Results: 1 - 10 of 133331 matches for " Ricardo Rom?o Guerra "
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Análises macro e microscópicas de enxertos cutaneos por semeadura após laserterapia de baixa intensidade Macro and microscopic analysis of island skin grafts after low-level laser therapy
Elisangela Barboza da Silva,Cintia Lúcia Maniscalco,Greyson Victor Zanatta ésper,Ricardo Romo Guerra
Revista do Colégio Brasileiro de Cirurgi?es , 2013,
Abstract: OBJETIVO: observar se a laserterapia de baixa intensidade acelera o processo inflamatório, a cicatriza o e epiteliza o de enxertos cutaneos por semeadura. MéTODOS: vinte ratos foram submetidos a esta técnica de enxertia e divididos em dois grupos iguais, um tratado com laser e outro controle. RESULTADOS: houve menor tempo de rea o inflamatória, maior velocidade de cicatriza o, epiteliza o e queratiniza o nos animais tratados com laser em rela o aos n o tratados. CONCLUS O: a laserterapia de baixa intensidade é efetiva no auxílio ao tratamento de enxertos por semeadura. OBJECTIVE: To observe the effects of low intensity laser therapy in inflammation, wound healing and epithelialization of island skin grafts. METHODS: Twenty rats were subjected to this grafting technique and divided subsequently into two equal groups, one treated with laser and the other control. RESULTS: there was less inflammation, faster healing, epithelialization and keratinization in the laser-treated animals when compared to the untreated. CONCLUSION: Low intensity laser therapy is helpful to island skin grafting.
Experimental models for induction of liver cirrhosis in animals: a review
Cristiane Carlin Passos,Amanda Olivotti Ferreira,Francisco Javier Hernandez Blazquez,Ricardo Romo Guerra
Biotemas , 2010,
Abstract: The liver plays a key role in the homeostatic balance of many biological processes. Cirrhosis is a syndrome in which chronic liver diseases converge, leading to hepatocellular injury, the exacerbated deposition of fibrous tissue, and eventually the disruption of the tissue architecture. The liver is subject to potential injury by a large quantity of pharmacological agents, toxic and/or microbiological. For the study of possible treatments for cirrhosis, it is necessary to establish animal models of induction of cirrhosis, especially in laboratory rodents which mimic the cirrhotic process found in animals and humans, that have high reproducibility and uniformity, with a low mortality rate. Thus, the induction of liver cirrhosis becomes essential to the investigation of chronic liver diseases, as well as to test possible therapeutic treatments for subsequent use in human and veterinary clinics. Currently, experimental studies have been conducted to collect data about the various hepatotoxic drug effects. Carbon tetrachloride -CCl4, Thioacetamide –TAA and dimethylnitrosamine -DMN were the drugs of choice for cirrhosis induction in experimental models in this study. The model using cirrhotic TAA seems to be the best model for the reason that it produces a histological pattern closest to that of human cirrhosis, leading to lower mortality with higher reproducibility and security, despite the longer period of induction (14 weeks).
Efeito da fotoperiodicidade na taxa de prenhez em ratos isogênicos (F344)
Baptista, Alexandre Bastos;Morita, Erika Lissa;Passos, Cristiane Carlin;Ferreira, Amanda Olivotti;Alves, Flávio Ribeiro;Alves, Alexandre José;Miglino, Maria Angélica;Guerra, Ricardo Romo;
Ciência Rural , 2011, DOI: 10.1590/S0103-84782011000100028
Abstract: the use of isogenic animals presents great experimental advantages, as phenotypic and genotypic uniformity (reducing the number of experimental animals) and histocompatibility, thus allowing, the accumulation of information, and the repeatability of the experiments. the isogenic strain of rattus norvegicus fischer 344 has existed for 90 years, however the reasons of its low reproductive index are not knew. the present study has demonstrated that fischer f344 rats are photoresponsive regarding reproduction, having improved its pregnancy index with the increase of the photoperiod. the best indexes were achieved when the males had been submitted to 14 hours of light and females to 16 hours of light, indicating sexual dimorphism in photoresponsivity.
Morfologia e desenvolvimento ultraestrutural do sistema renal de embri?es bovinos com idade gestacional entre 10 e 50 dias
Cagnoto, Daniela Gomes;Guerra, Ricardo Romo;Alberto, Miryan Vila;Ambrósio, Carlos Eduardo;Santos, José Manuel;Miglino, Maria Angélica;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782009005000155
Abstract: the aim of this study was to describe the bovine renal system development, during the early embryonic period (10-50 days). bovine embryos collected in abattoir were photographed and measured by crow-rump (cr) method to estimate the gestational age. the embryos destined to optical microscopy were fixed in bouin solution for development evaluations of renal system, as well as its structures. some embryos also were fixed in glutaraldeheyde 2.5% and destined to transmission electron microscopy for ultrastructural study of renal system cells. in bovine embryos with gestational age of 14 to 15 days (e14-15) the presence of pronephro were not observed, however the mesonephro was found, as well as morphologic indications of it is functional activity. mesonephro presented, in the interior of its tubular cells, innumerable mitochondrias and interdigitations, indicating high activity of ionic transportation. the metanephro, or definitive kidney, initiates its development in e23-24. the study demonstrated that the involution of mesonephro happened simultaneously with the metanephrogenic differentiation. in e45-46, the embryos already initiated the fetal phase, and the metanephro possess the filter units (nephrons), with its respective glomeruli, proximal and distal convoluted tubules, and the loop of henle.
Morfologia e funcionalidade do pneumócito tipo II e sua rela??o e varia??o com a idade gestacional em bovinos
Toquetti, Rita de Cássia;Guerra, Ricardo Romo;Ambrosio, Carlos Eduardo;Santos, José Manoel;Favaron, Phelipe Oliveira;Franciolli, André Luiz Rezende;Alberto, Miryan Vilia;Meirelles, Flávio Vieira;Miglino, Maria Angelica;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782009000800031
Abstract: the aim of this study was to characterize the presence of type ii pneumocyte and the beginning of the surfactant protein production in bovines to the correlated gestational age. lungs of fetuses at 4 months gestational age were found to be in the canalicular development phase, without presence of type ii pneumocytes or appearance of electrophoretic bands compatible with the presence of the surfactant protein. in fetuses at 5 months of gestational age, the lungs were at the terminal sac phase, with the presence of primitive alveolus, formed by cubical epithelium and areas formed by pneumocytes i and pneumocytes ii. no electrophoretic bands compatible to the surfactants proteins were identified. in contrast, lungs of fetuses at 6 months of gestational age showed, development of terminal sac phase, with the presence of type i and type ii pneumocytes. in this phase, proteic determination for the sds - page analysis showed the presence of bands between 26 to 36kda, demonstrating sp - a production, which is the surfactant protein found in highest amount. from the 7th gestational month on, the phase of terminal sac is more evident and complex, with intense sprouting vascularization. the type i pneumocyte had a more squamous aspect and type ii pneumocyte were more globular. in the sds - page analysis of the bronchial - alveolar wash, surfactant protein bands had been observed with a profile similar to that of the newborn animals. in newborn animals, lungs in alveolar phase showed the development of type i and ii pneumocytes. the profile of the bronchial - alveolar wash from the newborn was similar to an adult animal, presenting the same bands in triplets. these results suggest that early born fetuses, from 7 months of pregnancy could have a guarantee of surviving due to possible functionality of respiratory system because the lung presented all necessary features for surphactant protein syntheses. however, new clinical studies related to respiratory system functionality ope
Impact of Chronic Pelvic Pain on Female Sexual Function  [PDF]
Adriana Peterson Mariano Salata Romo, Ricardo Gorayeb, Gustavo Salata Romo, Omero Benedicto Poli-Neto, Antonio Alberto Nogueira
International Journal of Clinical Medicine (IJCM) , 2013, DOI: 10.4236/ijcm.2013.43031

The objective of the present study was to determine the prevalence of sexual dysfunction and depression in women with chronic pelvic pain (CPP). A case-control study was conducted on 66 women, 36 of them with CPP and 30 without this diagnosis. Depression was evaluated using the Beck Depression Inventory (BDI) and sexual dysfunction was evaluated using the Female Sexual Function Index (FSFI). Data were analyzed statistically by the Mann-Whitney test, Fisher exact test, chisquare test, and Spearman correlation test. Regarding sociodemographic data, no significant differences were detected between populations with respect to the variables studied (age, schooling, number of children, income, salary, and marital status), indicating group homogeneity and thus increasing the reliability of the data. A cut-off of 26.55 points was used to calculate the total score for sexual function. In the group of women with CPP, 94.4% were at high risk for sexual dysfunction. Comparison of FSFI scores showed that the domains of sexual function, such as orgasm, lubrication and pain differed significantly between women with and without CPPP. Correlations were detected between the following items: orgasm × age (r = -0.01904), orgasm × number of children (r =-0. 00947), orgasm × body mass index (BMI) (r =-0.00 955), relationship × age (r = 0.03952), income × relationship (r =-0.014680), relationship × number of children (r =-0.03623), depression × relationship (r =-0.16091), desire × age (r = -0.45255), desire × number of children (r = -0.01824), lubrication × excitement (r = 0.04198), and lubrication × BMI (r = -0.01608). The prevalence of depression detected in the present study was 38.9% among women with pain and 3.3% among control women. It was observed that women with CPP suffer a negative interference regarding sexual function compared to controls. Thus, it can be seen that a specific approach related to sexuality is extremely important within the context of women with CPP. Depression was clearly associated with CPP and therefore an interdisciplinary approach is fundamental in order to solve this problem.

The Impact of Chronic Pelvic Pain in Women  [PDF]
Adriana P. M. S. Romo, Ricardo Gorayeb, Gustavo Salata Romo, Omero Benedicto Poli-Neto, Antonio Alberto Nogueira
International Journal of Clinical Medicine (IJCM) , 2013, DOI: 10.4236/ijcm.2013.410076

Chronic pelvic pain (CPP) is a prevalent condition with a significant impact on the personal, social, professional and marital life of women. It is a complex condition that may have no specific causal diagnosis or may be associated with multiple diagnoses, frequently involving treatment failure. The definition of health care strategies fundamentally depends on the way women live with this condition. Thus, the objective of the present study is to learn how women with CPP experience their diagnosis and the meaning they attribute to it. A qualitative study was conducted by interviewing a focus group of 11 women. The content of the interviews was recorded and fully transcribed, and the speeches were interpreted by Bardin’s content analysis. The topics most frequently dealt with in the interview were diagnosis, beginning of pain, worsening and improving factors, marital and interpersonal relationships, interference with daily activities, association with emotional aspects, and perspectives for the future. It could be perceived how much these women need to be better heard and how much the association between psychic and physical questions must be visualized by the professionals who provide care for them. The approach used by professionals from different areas, when properly structured, can minimize the problem of the division of a sick person into separate parts. Psychological care is very important, especially in relation to the discovery of more effective strategies for living with pain.

Avalia??o cintilográfica de diferentes dosagens de Tecnécio-99m na padroniza??o da perfus?o pulmonar em c?es da ra?a Rottweiller
Rodrigues, Gabriela Silva;Guerra, Ricardo Romo;Alves, Flávio Ribeiro;Franciolli, André Luís Rezende;Favaron, Phelipe Oliveira;Marques, Fabio Luiz Navarro;Cuyumjian, Patricia Russano;Miglino, Maria Angélica;Tommasi Junior, Horácio Luis;Buchpiguel, Carlos Alberto;Bombonato, Pedro Primo;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010005000022
Abstract: pulmonary function evaluation in dogs can be gotten by scintigraphy exams, which are obtained with radiopharmacs injections, followed by radiography. in this study it was used 10 healthful male dogs of rottweiler breed, radiographically normal and serum negative for dirofilariasis, with administration of varied doses of human serum albumin grouped marked with technetium-99m (maa). the results were evaluated qualitatively and quantitatively, considering the index of pulmonary perfusion and its homogeneity, being found an increase on the homogeneity of image directly related with the number of maa particles injected. this study defines the criteria of normality and the dosage of pulmonary perfusion in healthful dogs established with scintigraphy technique.
Stability of Soybean Genotypes and Their Classification into Relative Maturity Groups in Brazil  [PDF]
José Elzevir Cavassim, Jo?o Carlos Bespalhok Filho, Luis Fernando Alliprandini, Ricardo Augusto de Oliveira, Edelclaiton Daros, Edson Perez Guerra
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.411258

The stability of soybean genotypes is very important in breeding programs for not only the evaluation, selection, and production of cultivars but also the establishment of parameters required for the classification of genotypes into relative maturity groups (RMG). The aim of this study was to define stable genotypes for traits, such as days to flowering, days to maturity, and length of the reproductive period, and to classify them into RMG. For this purpose, 20 commercial soybean cultivars were evaluated in 12 environments distributed in the major producing regions of Brazil. Assessments according to the Eberhart and Russell method and the additive main effects and multiplicative interaction (AMMI) method were effective in the identification of stable genotypes and their classification into RMG. These methods can also be used collectively for this purpose. Our results showed that the AMMI method led to a better interpretation of genotype-environment interactions. Thus, RMG obtained on the basis of stable genotypes represented a good estimate of the relative maturity of soybean crops throughout Brazil.

Tripanosomatides like Trypanosoma theileri in the cattle tick Boophilus microplus
Martins, Jo?o Ricardo;Leite, Romário C.;Doyle, Rovaina L.;
Revista Brasileira de Parasitologia Veterinária , 2008, DOI: 10.1590/S1984-29612008000200010
Abstract: findings of epimastigotes forms of a tripanosomatide is reported in the hemolymph of the cattle tick boophilus microplus in the state of rio grande do sul, southern brazil. morphological evidences suggest they are similar to trypanosoma theileri, a species described as non pathogenic to cattle, and usually transmitted by tabanids.
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