OALib Journal期刊

ISSN: 2333-9721



匹配条件: “Renesto” ,找到相关结果约32条。
Reproductive strategies and genetic variability in tropical freshwater fish
Lassala, Maria Dolores Peres;Renesto, Erasmo;
Genetics and Molecular Biology , 2007, DOI: 10.1590/S1415-47572007000400030
Abstract: we estimated the genetic variability of nine fish species from the brazilian upper paraná river floodplain (astyanax altiparanae, hoplias malabaricus, leporinus lacustris, loricariichthys platymetopon, parauchenipterus galeatus, pimelodus maculatus, rhaphiodon vulpinus, roeboides paranensis and serrasalmus marginatus) based on data for 36 putative allozyme loci obtained using corn starch gel electrophoresis of 13 enzymatic systems: aspartate aminotransferase (ec, acid phosphatase (ec, esterase (ec, glycerol-3-phosphate dehydrogenase (ec, glucose-6-phosphate dehydrogenase (ec, glucose-6-phosphate isomerase (ec, iditol dehydrogenase (ec, isocitrate dehydrogenase - nadp+ (ec, l-lactate dehydrogenase (ec, malate dehydrogenase (ec, malate dehydrogenase-nadp+ (ec, phosphoglucomutase (ec and superoxide dismutase, (ec the mean expected heterozygosity varied from zero to 0.147. when data from the literature for 75 species of tropical fish were added to the nine species of this study, the heterozygosity values differed significantly among the groups of different reproductive strategies. the highest mean heterozygosity was for the non-migratory without parental care, followed by the long-distance migratory, and the lowest mean was for the non-migratory with parental care or internal fecundation.
Validation of a Marker of Atrial Contraction in the SonR Signal  [PDF]
Florent Broussous, Adonis Kobeissi, Jér?me Dumont, Fabrizio Renesto, Jacques Mansourati
World Journal of Cardiovascular Diseases (WJCD) , 2015, DOI: 10.4236/wjcd.2015.53008
Abstract: Introduction: The main component of the endocardial acceleration signal (SonR) is today used for cardiac resynchronization therapy (CRT) optimization. This prospective, single center pilot study focuses on another signal component, SonR4 that may provide further information on the atrial activity. Methods and Results: SonR signal and ECG tracings were recorded simultaneously during a CRT-D optimization procedure in 15 patients (12 men, 68 ± 9.5 years, ischemic heart disease 53%) indicated for CRT. Correlation between SonR4 signal, recorded using SonR and atrial contraction, identified by Echo Doppler was evaluated by Pearson and Student’s t tests under different Atrio-Ventricular (AV) delay programming. From 15 consecutive screened patients, 9 had concomitant analyzable SonR4 and ECG recordings and were included in the study population. The presence of the SonR4 component was systematically correlated to the presence of the A wave. A significant correlation was observed between SonR4 and A wave timings (r = 0.75, p = 0.02) according to different AV delays, with a high reproducibility in SonR4 assessment. Conclusion: A strong correlation between SonR4 and atrial contraction timings was observed, further suggesting that SonR4 is a marker of the atrial contraction. Additional assessments in larger populations are required to confirm these results and build further applications.
Genetic variability in three species of Gymnotus Linnaeus, 1758 (Gymnotiformes: Gymnotidae) from Caracu stream of the upper Paraná River basin, Brazil
Rezende, Judy Ruiz;Renesto, Erasmo;Zawadzki, Cláudio Henrique;
Neotropical Ichthyology , 2009, DOI: 10.1590/S1679-62252009000400010
Abstract: the genetic variability of three gymnotus species from the caracu stream, a small tributary of the left margin of paraná river (brazilian upper paraná river floodplain), was estimated with data of 17 putative allozyme loci, which were obtained by using corn starch gel electrophoresis of 10 enzymatic systems: aspartate aminotransferase (e. c., alcohol dehydrogenase (e. c., esterase (e. c., glucose dehydrogenase (e. c., glycerol-3-phosphate dehydrogenase (e. c., isocitrate dehydrogenase (e. c., l-lactate dehydrogenase (e. c., malate dehydrogenase (e. c., superoxide dismutase (e. c. and sorbitol dehydrogenase (e. c. the genetic diversity was estimated as he = 0.3458 for g. pantanal, he = 0.2481 for g. inaequilabiatus, and he = 0.3152 for g. sylvius. the most divergent species were g. sylvius and g. pantanal (d = 0.117), and the most similar were g. inaequilabiatus and g. pantanal (d = 0.051). the data indicates that the observed genetic variability was very low and the expected variability estimated for these three species is very high, and the genetic differences among them are small. the data suggest that the process of speciation which produced these three species is recent.
Biochemical comparison of two Hypostomus populations (Siluriformes, Loricariidae) from the Atlantico Stream of the upper Paraná River basin, Brazil
Ito, Kennya F.;Renesto, Erasmo;Zawadzki, Cláudio H.;
Genetics and Molecular Biology , 2009, DOI: 10.1590/S1415-47572009000100008
Abstract: two syntopic morphotypes of the genus hypostomus - h. nigromaculatus and h. cf. nigromaculatus (atlantico stream, paraná state) - were compared through the allozyme electrophoresis technique. twelve enzymatic systems (aat, adh, est, gcdh, g3pdh, gpi, idh, ldh, mdh, me, pgm and sod) were analyzed, attributing the score of 20 loci, with a total of 30 alleles. six loci were diagnostic (aat-2, gcdh-1, gpi-a, idh-1, ldh-a and mdh-a), indicating the presence of interjacent reproductive isolation. the occurrence of few polymorphic loci acknowledge two morphotypes, with heterozygosity values he = 0.0291 for h. nigromaculatus and he = 0.0346 for h. cf. nigromaculatus. fis statistics demonstrated fixation of the alleles in the two morphotypes. genetic identity (i) and distance (d) of nei (1978) values were i = 0.6515 and d = 0.4285. the data indicate that these two morphotypes from the atlantico stream belong to different species.
Genetic variability of Hypostomus (Teleostei, Loricariidae) from the Ribeir?o Maringá, a stream of the upper Rio Paraná basin, Brazil
Paiva, Suzana de;Renesto, Erasmo;Zawadzki, Cláudio Henrique;
Genetics and Molecular Biology , 2005, DOI: 10.1590/S1415-47572005000300005
Abstract: hypostomus strigaticeps and two morphotypes of hypostomus were collected from ribeir?o maringá, a small tributary of the rio pirapó, an effluent of the upper rio paraná. the three populations were analyzed by allozyme electrophoresis that allowed the scoring of 25 loci from 14 enzyme systems. heterozygosity values (he) were 0.028 in h. strigaticeps, 0.027 in hypostomus sp. 1 and zero in hypostomus sp. 2. several diagnostic loci and fixed differences were observed for each population at loci acp-a, gcdh-a and mdhp-a. thus, all populations were genetically distinct, although there were many common alleles. the unbiased genetic identities of nei (i) were estimated as 0.780 for hypostomus sp. 1 and h. strigaticeps, 0.357 for h. strigaticeps and hypostomus sp. 2 and 0.322 for hypostomus sp. 1 and hypostomus sp. 2. the data indicate that the two morphotypes are distinct species from hypostomus strigaticeps.
Allozyme comparison of two populations of Rineloricaria (Siluriformes, Loricariidae) from the Ivaí River, upper Paraná River basin, Brazil
Limeira, Daniel M.;Renesto, Erasmo;Zawadzki, Cláudio H.;
Genetics and Molecular Biology , 2009, DOI: 10.1590/S1415-47572009005000022
Abstract: two allopatric morphotypes of the genus rinelocaria were compared through the allozyme electrophoresis technique: one morphotype, r. pentamaculata, from the keller river in the middle stretch of the ivaí river basin and the other, r. aff. pentamaculata, from the s?o jo?o river in the upper portion of the ivaí river basin. the morphotype from the s?o jo?o river was collected upstream from the s?o jo?o waterfall, which is about 80 m deep. twelve enzymatic systems (aat, adh, est, gcdh, g3pdh, gpi, idh, ldh, mdh, me, pgm and sod) were analyzed, which allowed to score 22 loci. only loci aat-2, est-3 and mdh-c showed polymorphism. the two samples differed in allele frequencies at the three polymorphic loci. the average expected heterozygosity for all loci was 0.0806 ± 0.0447 in the keller river sample. for the s?o jo?o river morphotype, this value was 0.0489 ± 0.0350. nei' s genetic identity and distance between the two populations were respectively 0.9789 and 0.0213. wright's fis, fit and fstover all loci were estimated as 0.3121, 0.4021 and 0.1309, respectively. we consider that the two morphotypes represent species in statu nascendi.
Genetic variability in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the Upper Paraná River basin, Brazil
Peres, Maria Dolores;Vasconcelos, Marcio dos Santos;Renesto, Erasmo;
Genetics and Molecular Biology , 2005, DOI: 10.1590/S1415-47572005000500011
Abstract: allozyme data was used to assess the genetic diversity astyanax altiparanae populations from the floodplain of the upper paraná river (pr). specimens were collected in the southern brazilian state of paraná from pr in porto rico municipality and ribeir?o ficha (rf) in ubirat? municipality. the authors used 15% (w/v) corn starch gel electrophoresis to identify 21 putative loci for 13 enzymatic systems: aspartate aminotransferase, (aat), acid phosphatase, (acp), esterase, (est), glycerol-3-phosphate dehydrogenase, (g3pdh), glucose-6-phosphate dehydrogenase, (g6pdh), glucose-6-phosphate isomerase, (gpi), iditol dehydrogenase, (iddh), isocitrate dehydrogenase - nadp+, (idh), l-lactate dehydrogenase, (ldh), malate dehydrogenase, (mdh), malate dehydrogenase - nadp+, (mdhp), phosphoglucomutase, (pgm), and superoxide dismutase, (sod). the proportion of polymorphic loci were estimated as 52.38% in the pr population and 38.10% in the rf population. expected estimated heterozygosities were 0.1518 ± 0.0493 for the pr population and 0.0905 ± 0.0464 for the rf population. the a. altiparanae heterozygosity data were similar to previous estimates for other pr basin characid species. allele frequencies were significantly different between the pr and rf populations in respect to some loci (acp-1, g3pdh-1, gpi-a, iddh-1, mdhp-1 and mdhp-2). wright?s statistics for all loci were estimated as fis = 0.3919, fit = 0.4804 and fst = 0.1455. our results show that the a. altiparanae populations studied are genetically different and have a high degree of genetic variability.
Genetic evidence for two species of the genus Pimelodus Lacépède, 1803 (Siluriformes, Pimelodidae) in the Igua?u River (Brazil)
Renesto, Erasmo;Zawadzki, Cláudio Henrique;Revaldaves, Eloísa;
Genetics and Molecular Biology , 2000, DOI: 10.1590/S1415-47572000000400017
Abstract: the existence of reproductive isolation between two morphs of catfish, endemic to the igua?u river (brazil), was examined by enzyme starch gel electrophoresis. tissues of 19 catfish (pimelodus ortmanni) and 15 of a similar morph (pimelodus sp.), which differs from p. ortmanni by presenting larger and more scattered dusky spots on its skin, were analyzed. a nei's (1978) genetic identity of 0.551 was determined by the analysis of 22 enzyme loci. the loci est*1, est*2, gdh*1, gpi*1, gpi*2, idh*1, mdh*1, mdh*2, and pgm*1 were fixed for different alleles in each morph, that is, no heterozygote was found for these loci. the enzymatic patterns observed for the two morphs indicate both that the taxa are reproductively isolated and that they in fact represent separate species.
Cross-linking e segmento de anel corneano intraestromal
Renesto, Adimara da Candelaria;Sartori, Marta;Campos, Mauro;
Arquivos Brasileiros de Oftalmologia , 2011, DOI: 10.1590/S0004-27492011000100017
Abstract: corneal cross-linking is a procedure used for stabilizing the cornea in patients with progressive keratoconus by increasing corneal rigidity, and it is also used in corneal inflammatory melting process. the intrastromal corneal ring segments act by flatting the center of the cornea. originally designed for the correction of mild myopia, the segments are now being used for reduction of keratoconus in order to improve the uncorrected visual acuity, the best spectacle corrected visual acuity, to allow good tolerance to the use of contact lenses and delay the need for corneal grafting procedures. the present text presents a review of corneal cross-linking and insertion of intrastromal corneal ring segments, emphasizing their indications, results and complications related until now.
Impression cytologic analysis after corneal cross-linking and insertion of corneal ring segments for keratoconus: two-year results
Renesto, Adimara da Candelaria;Barros, Jeison de Nadai;Campos, Mauro;
Arquivos Brasileiros de Oftalmologia , 2012, DOI: 10.1590/S0004-27492012000200006
Abstract: purpose: to report the impression cytologic results after corneal cross-linking and insertion of intrastromal corneal ring segments for keratoconus. methods: thirty-nine eyes were distributed into two groups: 1) cross-linking group (patients underwent corneal cross-linking procedure), and 2) riboflavin eyedrops group (patients received 0.1% riboflavin (w/v) eyedrops in 20% dextran solution for 1 month). after 3 months, all patients underwent insertion of intrastromal corneal ring segments. impression cytologic specimens were obtained from all eyes at baseline, at 1 month and 3 months after cross-linking or riboflavin eyedrops, and again at 6 months, 1 year, and 2 years after intrastromal corneal ring segment insertion. results: patients in the cross-linking group demonstrated improvement in the cell-to-cell contact of epithelial cells and the nucleus-to-cytoplasm ratio on the temporal conjunctiva after treatment (p=0.008 and p=0.047), respectively. on the superior conjunctiva, increases in goblet cell density (p=0.037) and level of organization of nuclear chromatin (p=0.010) after treatment were noted. patients in the riboflavin eyedrops group demonstrated improvement in the cell-to-cell contact of epithelial cells on the superior conjunctiva after treatment (p=0.021). on the temporal conjunctiva, an improvement in the cell-to-cell contact of epithelial cells (p<0.001) and increases in the nucleus-to-cytoplasm ratio (p<0.001), goblet cell density (p=0.001), and less keratinization (p=0.011) were noted. no changes were identified on the cornea for either group. fisher's exact test comparison of the impression cytologic total scores after treatment revealed no difference between groups. conclusion: despite changes in some conjunctival parameters (e.g., cell-to-cell contact of epithelial cells, nucleus-to-cytoplasm ratio, level of organization of nuclear chromatin, goblet cell density, and keratinization), comparison of the total impression cytologic scores revealed no

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