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Search Results: 1 - 10 of 227048 matches for " Renata Cássia-Pires "
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Distinct Leishmania Species Infecting Wild Caviomorph Rodents (Rodentia: Hystricognathi) from Brazil
Renata Cássia-Pires,Mariana C. Boité,Paulo S. D'Andrea,Heitor M. Herrera,Elisa Cupolillo,Ana Maria Jansen,André Luiz R. Roque
PLOS Neglected Tropical Diseases , 2014, DOI: 10.1371/journal.pntd.0003389
Abstract: Background Caviomorph rodents, some of the oldest Leishmania spp. hosts, are widely dispersed in Brazil. Despite both experimental and field studies having suggested that these rodents are potential reservoirs of Leishmania parasites, not more than 88 specimens were analyzed in the few studies of natural infection. Our hypothesis was that caviomorph rodents are inserted in the transmission cycles of Leishmania in different regions, more so than is currently recognized. Methodology We investigated the Leishmania infection in spleen fragments of 373 caviomorph rodents from 20 different species collected in five Brazilian biomes in a period of 13 years. PCR reactions targeting kDNA of Leishmania sp. were used to diagnose infection, while Leishmania species identification was performed by DNA sequencing of the amplified products obtained in the HSP70 (234) targeting. Serology by IFAT was performed on the available serum of these rodents. Principal findings In 13 caviomorph rodents, DNA sequencing analyses allowed the identification of 4 species of the subgenus L. (Viannia): L. shawi, L. guyanensis, L. naiffi, and L. braziliensis; and 1 species of the subgenus L. (Leishmania): L. infantum. These include the description of parasite species in areas not previously included in their known distribution: L. shawi in Thrichomys inermis from Northeastern Brazil and L. naiffi in T. fosteri from Western Brazil. From the four other positive rodents, two were positive for HSP70 (234) targeting but did not generate sequences that enabled the species identification, and another two were positive only in kDNA targeting. Conclusions/Significance The infection rate demonstrated by the serology (51.3%) points out that the natural Leishmania infection in caviomorph rodents is much higher than that observed in the molecular diagnosis (4.6%), highlighting that, in terms of the host species responsible for maintaining Leishmania species in the wild, our current knowledge represents only the “tip of the iceberg.”
Chemical Fingerprinting of Counterfeits of Viagra and Cialis Tablets and Analogues via Electrospray Ionization Mass Spectrometry  [PDF]
Rafael S. Ortiz, Kristiane de Cássia Mariotti, Wanderson Rom?o, Marcos N. Eberlin, Renata P. Limberger, Paulo Mayorga
American Journal of Analytical Chemistry (AJAC) , 2011, DOI: 10.4236/ajac.2011.28106
Abstract: Direct infusion electrospray ionization mass spectrometry (ESI-MS) is shown to provide an efficient tech- nique for the investigation of polar composition of forensic tablets for male erectile dysfunction. ESI-MS fingerprinting of 41 commercial sildenafil samples (Viagra®, Cialis®, Lazar®, Libiden®, , Maxfil®, , Plenovit®, Potent 75®, Rigix®, V-50®, Vimax®, Pramil 75® and Pramil®) and 56 counterfeit samples (Viagra and Cialis) were obtained. The spectra for the authentic Viagra® tablets showed abundant ions exclusively corresponding to the sildenafil (SLD) molecule: [SLD + H]+ of m/z 475; [SLD + Na]+ of m/z 497; and [2SLD + H]+ of m/z 949. The spectra for com-mercial sildenafil samples also showed predominat SLD ions. Tablets of authentic Cialis® showed mainly ions of m/z 343, 365 and 707 from the lactose molecule (the excipient); as well as a minor ion of m/z 390 corresponding to the active ingredient tadalafil (TAD) in its protonated form [TAD + H]+. For counterfeit Cialis samples, how-ever, normally TAD ions of much high abundances was observed, together with ions corresponding to sildenafil analogues such as those of m/z 489 (homosildenafil) and 505 (hydroxyhomosildenafil). Principal component analysis was applied to ESI-MS fingerprint data, placing samples according to their contents of active ingredients hence authentic and counterfeit samples are easily recognized.
Sodium and health: New proposal of distribution for major meals  [PDF]
Renata Puppin Zandonadi, Raquel B. A. Botelho, Ver?nica C. Ginani, Rita de Cássia C. A. Akutsu, Karin Eleonora de Oliveira Savio, Wilma M. C. Araújo
Health (Health) , 2014, DOI: 10.4236/health.2014.63029
Abstract: Sodium ingestion in regular diet exceeds individuals’ physiological needs, and it is etiologically related to various diseases. Studies estimate that the worldwide average individual intake of sodium reaches almost double the maximum limit of ingestion for the day only in the main meals: lunch and dinner. Culturally, sodium is highly used in meals to enhance the flavor of preparations. Then, the purposes of this research are to evaluate sodium use in meals prepared in Popular Brazilian Restaurants and to suggest the distribution of sodium for each kind of preparation of major meals. This is an exploratory cross-sectional research based on direct documentation subdivided in six steps: determination of samples of foodservices and menus; development of the technical preparation files offered in the menus; determination of the weight of average portions offered; determination of the average amount of sodium in the portions offered; estimation of sodium distribution regarding two parameters; and determination of the sodium distribution among preparations of major meals by the two proposed parameters. For results’ analysis, portions were calculated as well as the mean contribution of the preparation in the plate weight in percentage and the mean sodium contribution. Standard deviation is also presented. We verified through the study the possibility of using the distribution of sodium by two parameters: the first one considers the ratio of components’ weight in the menu in relation to the total weight of the meal; the second one considers the distribution of sodium in preparations in relation to the total amount of sodium in the meal. We expect the results of this research to improve menu planning in restaurants as to fulfill the actual clients’ needs in order to prevent diseases.
Efeitos do thidiazuron e do ácido giberélico nas características dos cachos e bagas de uvas 'niagara rosada' na regi?o de Jundiaí-SP
Botelho, Renato Vasconcelos;Pires, Erasmo José Paioli;Terra, Maurilo Monteiro;Carvalho, Cássia Regina Limonta;
Revista Brasileira de Fruticultura , 2003, DOI: 10.1590/S0100-29452003000100028
Abstract: two trials were carried out in vineyards located in jundiaí-sp, with the objective of studying the effects of growth regulators applications on 'niagara rosada' grapes. in the first trial, it was used thidiazuron (tdz) at 5 or 10mg.l-1, and/or gibbellic acid at 100mg.l-1. in the second experiment, the following doses of thidiazuron were used: 0; 2.5, 5.0, 7.5, 10.0, 12.5 and 15.0mg.l-1. the growth regulators were applied by dipping the cluster 14 days after full blooming. it was evaluated weight, length and width of clusters and berries; rachis weight; number of berries; number of seeds; total soluble solutes content and total titratable acidity of juice. in the first experiment, the highest weight, length and width of berries were verified to the treatments with thidiazuron associated to gibberellic acid. treatments with only gibberellic acid was not effective in increasing berry size and reduced number of berries per cluster. in the second trial, thidiazuron applications increased linearly weight of rachis and cluster; and weight, length and width of berries. there weren't significant differences for total soluble solutes content, total titratable acidity, number of seeds and cluster width.
The attitudes of nurses from an intensive care unit in the face of errors: an approach in light of bioethics
Coli, Rita de Cássia Pires;Anjos, Marcio Fabri dos;Pereira, Luciane Lucio;
Revista Latino-Americana de Enfermagem , 2010, DOI: 10.1590/S0104-11692010000300005
Abstract: this study analyzed the attitudes of nurses concerning the occurrence of errors in nursing procedures carried out in an intensive care unit (icu) based on the bioethics framework. this descriptive study with qualitative approach was carried out with 14 nurses from a private hospital in the city of s?o paulo, brazil. results were analyzed according to bardin’s proposal of content analysis. the resulting themes were: acknowledging one’s fallibility; acknowledging and reporting errors; hiding errors. the nurses’ reports are based on considerations through the lens of bioethics: taking responsibility for an error implies acknowledging one’s own vulnerabilities; acknowledging an error with responsibility implies ethical conditions in the relationships among those involved; and errors are in the context of a particular environment. this study enables re-thinking nursing practice based on bioethics, resorting to the analysis of errors focusing on the relationships between those involved.
Efeitos do thidiazuron e do ácido giberélico nas características dos cachos e bagas de uvas 'niagara rosada' na regi o de Jundiaí-SP
Botelho Renato Vasconcelos,Pires Erasmo José Paioli,Terra Maurilo Monteiro,Carvalho Cássia Regina Limonta
Revista Brasileira de Fruticultura , 2003,
Abstract: Dois experimentos foram conduzidos em vinhedo localizado na regi o de Jundiaí-SP, com o objetivo de estudar-se os efeitos de aplica es de fitorreguladores em uvas 'Niagara Rosada'. No primeiro ensaio, utilizou-se de thidiazuron na concentra o de 5 ou 10 mg.L-1, e/ou ácido giberélico na concentra o de 100 mg.L-1. No segundo experimento, as doses de thidiazuron foram: 0; 2,5; 5,0; 7,5; 10,0; 12,5 e 15,0 mg.L-1. Os fitorreguladores foram aplicados mediante única imers o dos cachos em solu o, 14 dias após a plena flora o. Foram avaliados massa, largura e comprimento dos cachos e bagas; massa dos enga os; número de bagas; número de sementes; teor de sólidos solúveis totais e acidez total titulável do mosto. No primeiro ensaio, os maiores valores de massa, comprimento e largura das bagas foram verificados para os tratamentos com thidiazuron associado a ácido giberélico. O tratamento isolado de ácido giberélico n o foi efetivo para aumentar o tamanho das bagas e reduziu o número de bagas por cacho. No segundo experimento, aplica es de thidiazuron aumentaram linearmente a massa de cachos e enga os, e a massa, largura e comprimento das bagas. As variáveis teor de sólidos solúveis totais, acidez total titulável, número de sementes e largura de cachos n o foram influenciadas pelo TDZ e pelo AG3.
Anti-VP1 and anti-VP2 antibodies detected by immunofluorescence assays in patients with acute human parvovirus B19 infection
Pereira, Renata FA;Paula, Walquiria NS de;Cubel, Rita de Cássia N;Nascimento, Jussara P;
Memórias do Instituto Oswaldo Cruz , 2001, DOI: 10.1590/S0074-02762001000400011
Abstract: acute human parvovirus b19 infection is followed by an antibody response to the structural proteins of the viral capsid (vp1 and vp2). we used 80 sera collected from 58 erythema infectiosum and 6 transient aplastic crisis patients to test igm and igg antibodies against these two proteins in an immunofluorescence assay (ifa) using sf9 cells infected with recombinant baculovirus expressing either vp1 or vp2 antigen. although less sensitive than igm capture enzyme immunoassay using native antigen (maceia), we could detect anti-vp1 or anti-vp2 igm antibodies by ifa in 49 patients with acute infection (76.6%). detection of igg anti-vp1 and anti-vp2 by ifa, however, was as sensitive as igg detection by indirect enzyme immunoassay. by applying igg avidity ifa to sera of the 15 igm ifa negative patients we were able to confirm acute infection in further 12 cases by ifa. overall, acute infection was confirmed by ifa in 61 (95.3%) of the 64 patients.
Determina??o de carbamato de etila em aguardentes de cana por CG-EM
Labanca, Renata Adriana;Glória, Maria Beatriz Abreu;Afonso, Robson José de Cássia Franco;
Química Nova , 2008, DOI: 10.1590/S0100-40422008000700044
Abstract: a simple, specific and sensitive gc-ms procedure with ion m/z 62 was validated for the determination of ethyl carbamate (ec) in spirits. it exhibited linearity over the concentration of 30 to 600 μg/l with 30 μg/l limit of quantification. ec was detected in 70 of the 71 samples analyzed with levels from 33 to 2609 μg/l (mean level = 893 μg/l). 35% of the samples contained 500 to 1000 μg/l and 23% contained 150 to 500 and 1000 to 1500 μg/l. no significant correlation was found between ec and the levels of copper, ph and alcohol content of the samples.
Visita domiciliária: um olhar da enfermagem psiquiátrica
Labate, Renata Curi;Galera, Sueli Aparecida Frari;Avanci, Rita de Cássia;
Revista Brasileira de Enfermagem , 2004, DOI: 10.1590/S0034-71672004000500024
Abstract: the home visit is a matter of concern in diverse areas of nursing. with the implementation of psychiatric services in the community, the need for visits has become evident. for one year, we were involved with this activity, requesting it or providing it for patients assisted in the núcleo de saúde mental (mental health center). the aim of this paper is to describe our experience, detailing the following aspects: the public and private life of the family and the collaboration between professionals and resources in the community. home visits bring the team closer to the diseased person's context and favor articulation among him/her, his/her family, the health center and community resources.
Influência do alimento na produ??o e qualidade do leite de cabra
Costa, Roberto Germano;Queiroga, Rita de Cássia R. E.;Pereira, Renata A. G.;
Revista Brasileira de Zootecnia , 2009, DOI: 10.1590/S1516-35982009001300031
Abstract: goat milk is considered one of the most complete foods, since it holds several elements which are important for human nutrition, and necessary for the constitution of tissues and blood. this revision aims to assess the variations of the chemical composition and physical-chemical properties of the milk which occur due to some factors, such as: climate, breed, the individual, lactation stage and food. feed management has been considered a key factor in the manipulation of milk components. there is a general understanding that among the components of the milk fat is the most influenced by food. the use of molecular biological techniques might contribute to the understanding of the mechanism of utilization of food in rumen, establishing a new possibility for ruminal microbiology, which can be different from what has been studied so far.
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