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Search Results: 1 - 10 of 4608 matches for " Raquel Logullo "
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Immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitis
Bertevello, Pedro L.;Logullo, ?ngela Flávia;Nonogaki, Sueli;Campos, Fabio M.;Chiferi, Valcir;Alves, Claudia C.;Torrinhas, Raquel S.;Gama-Rodrigues, Joaquim José;Waitzberg, Dan L.;
Clinics , 2005, DOI: 10.1590/S1807-59322005000400004
Abstract: experimental colitis induced by acetic acid has been used extensively as a model for intestinal inflammatory disease. colonic tissue lesions of intestinal inflammatory disease patients seem to be related to the increased local production of pro-inflammatory cytokines (il-1, il-6, tnf-a, and ifn-g). purpose: to assess the cytokine expression pattern identified through immunohistochemistry in colonic mucosa after experimental colitis induced by acetic acid and establish the relationship between this pattern and the presence of macroscopic lesions. materials and methods: adult male wistar rats (n = 39) were divided at random into 4 groups: nc45 and nc24 (control without colitis; sacrificed at 45 minutes and 24 hours, respectively); and wc45 and wc24 (with experimental colitis induced by acetic acid; sacrificed at 45 minutes and 24 hours, respectively). macroscopic and microscopic alterations in colonic tissue were evaluated, and cytokine expression was assessed through immunohistochemistry. results: after 24 hours, il-1 expression was greater in the groups with colitis when compared to the groups without colitis. il-4 expression was higher in the wc45 group. there was an increase in both inf-g and il-6 related to the presence of necrosis of the colonic mucosa in the groups with colitis for both periods evaluated. conclusion: the immunohistochemical technique was efficient for the analysis of various cytokine expressions in the colonic tissue. there was an increase in the il-1 pro-inflammatory cytokines as well as in il-6 and ifn-g associated with the presence of colonic necrosis. experimental colitis induced by acetic acid is a useful model for the development of studies assessing the role of cytokines in the inflammation of mucosa as well as anti-cytokine therapies.
Economic Viability of Semi-Confined and Confined Milk Production Systems in Free-Stall and Compost Barn  [PDF]
Raquel Breitenbach
Food and Nutrition Sciences (FNS) , 2018, DOI: 10.4236/fns.2018.95046
Abstract: The objective of this research was to survey the productivity, costs, agricultural income and economic viability of Semi-Confined and confined milk production systems in Free-Stall and Compost Barn in Rio Grande do Sul, Brazil. In order to perform the research, four milk production units were selected in each of the three systems studied. Compared to the others, the Semi-Confined system had greater return of capital invested per year, as well as higher agricultural income per animal and per area, while the confined system in Compost Barn had higher farm incomes per man work unit and total farm incomes due to greater productive scale of properties. The Free-Stall system had the worst economic results. It was not possible to define an ideal system for the state, since the specificities of each property should be analyzed for choosing the system, especially the availability of the factors of production land, capital and labor.
The changes on intimate partner violence against women in the health system in Portugal  [PDF]
Raquel Cardoso, José Ornelas
Health (Health) , 2013, DOI: 10.4236/health.2013.57A4010

In Portugal, over the last 20 years, we have seen great strides in addressing intimate partner violence against women by their male partners. Measuring and understanding progress, and achieving sustainable systems change is a major challenge and to achieve this aim we need to identify events, persons and settings that contribute for the critical factors that create long term transformations in the systems and the creation of new resources [1]. Since the healthcare system is one of the main actors of intervention with survivors, the goal of this project was to interview key stakeholders from the healthcare system in order to understand the main changes they perceive have happened over the past two decades. Based on interviews with key stakeholders, our study explores the evidences of the changes that occurred in the healthcare system, the intersections with other systems and their visions for the future.

Epigenetics and Depression: A Rabbit Hole of Discovery  [PDF]
Raquel Pace, Renald Blundell
Open Journal of Genetics (OJGen) , 2018, DOI: 10.4236/ojgen.2018.83007
Abstract: “After this, there is no turning back. You take the blue pill—the story ends, you wake up in your bed and believe whatever you want to believe. You take the red pill—you stay in Wonderland, and I show you how deep the rabbit hole goes. Remember: all I’m offering is the truth.” Epigenetics is a bit like the red pillthe more it is researched the further down the rabbit hole we are goingthe realisation that my choices today as my parents’ and grandparents’ choices yesterday influence who I am now and who I am going to be tomorrow. Through the study of gene influencers, scientists are discovering that it is not only the DNA that makes us but which portion of is selected for use that tells us who we are. In the field of depression, epigenetics is still in its infancy, nonetheless significant connections have been uncovered. This paper gives a brief overview on epigenetics and the different mechanisms correlated with it. It also discusses factors affecting an individual’s epigenetic patterns and the effect of epigenetics itself. It specifically delves into the epigenetic effects on psychiatry, mainly depression, depressive symptoms and antidepressant treatments and the mediation of mechanism of action of the epigenetic modifications involved.
Intravenous Immunoglobulin and Mycophenolate Mofetil for Long-Standing Sensory Neuronopathy in Sj?gren's Syndrome
Maria Giovanna Danieli,Lucia Pettinari,Ramona Morariu,Fernando Monteforte,Francesco Logullo
Case Reports in Immunology , 2012, DOI: 10.1155/2012/186320
Abstract: Sensory neuronopathy is described in association with the Sjögren's syndrome (SS). We studied a 55-year-old woman with a 4-year history of progressive asymmetric numbness, distal tingling, and burning sensation in upper and lower limbs. In a few months, she developed ataxia with increased hypoanaesthesia. Electrodiagnostic tests revealed undetectable distal and proximal sensory nerve action potential in upper and lower limbs. Cervical spine magnetic resonance showed a signal hyperintensity of posterior columns. Previous treatment with high-dose glucocorticoids and azathioprine was ineffective. A combined treatment with intravenous immunoglobulin and mycophenolate mofetil was followed by a progressive and persistent improvement. This case documented the efficacy and the safety of the coadministration of intravenous immunoglobulin and mycophenolate mofetil in sensory neuronopathy associated with SS refractory to conventional immunosuppressive therapy.
Germ band retraction as a landmark in glucose metabolism during Aedes aegypti embryogenesis
Wagner Vital, Gustavo Rezende, Leonardo Abreu, Jorge Moraes, Francisco JA Lemos, Itabajara Vaz, Carlos Logullo
BMC Developmental Biology , 2010, DOI: 10.1186/1471-213x-10-25
Abstract: Glucose metabolism was investigated throughout Aedes aegypti (Diptera) embryonic development. Both cellular blastoderm formation (CBf, 5 h after egg laying - HAE) and germ band retraction (GBr, 24 HAE) may be considered landmarks regarding glucose 6-phosphate (G6P) destination. We observed high levels of glucose 6-phosphate dehydrogenase (G6PDH) activity at the very beginning of embryogenesis, which nevertheless decreased up to 5 HAE. This activity is correlated with the need for nucleotide precursors generated by the pentose phosphate pathway (PPP), of which G6PDH is the key enzyme. We suggest the synchronism of egg metabolism with carbohydrate distribution based on the decreasing levels of phosphoenolpyruvate carboxykinase (PEPCK) activity and on the elevation observed in protein content up to 24 HAE. Concomitantly, increasing levels of hexokinase (HK) and pyruvate kinase (PK) activity were observed, and PEPCK reached a peak around 48 HAE. Glycogen synthase kinase (GSK3) activity was also monitored and shown to be inversely correlated with glycogen distribution during embryogenesis.The results herein support the hypothesis that glucose metabolic fate changes according to developmental embryonic stages. Germ band retraction is a moment that was characterized as a landmark in glucose metabolism during Aedes aegypti embryogenesis. Furthermore, the results also suggest a role for GSK3 in glycogen balance/distribution during morphological modifications.The mosquito Aedes aegypti is vector of urban yellow fever and also the main dengue vector [1]. One of the major problems involving dengue transmission is that A. aegypti embryos enter dormancy at the end of embryogenesis, surviving and remaining viable for several months inside the egg [2-4]. This extended viability is possible due to the acquisition of embryonic desiccation resistance, a biological mechanism that is believed to involve the formation and maturation of serosal cuticle, a layer covering the embryo [5]. De
Assessment of angiogenesis expression and its relationship with prognosis of colorectal cancer by conventional and computer-assisted histopathological image analysis
Leme, Marcelo Betim Paes;Waitzberg, ?ngela Flavia Logullo;Artigiani Neto, Ricardo;Linhares, Marcelo Moura;Matos, Delcio;
Acta Cirurgica Brasileira , 2006, DOI: 10.1590/S0102-86502006000600007
Abstract: purpose: to quantify the degree of angiogenesis by conventional method (microvessel density, mvd) and computerized method (endothelial area, ea), and to evaluate their relationships with the prognosis of patients operated on for colorectal adenocarcinoma. methods: tumoral angiogenesis was studied by means of an immunohistochemical technique, using cd 34, on 126 patients; to quantify the angiogenesis, mvd (defined as number of microvessels per mm2) and ea measurement (defined as the area occupied by ea in the microscope field). a computerized method, imagelab software was utilized to quantify endothelial area. results: the mean number of microvessels was 128.6 mv/mm2 (sd = 44.5) and the mean ea was 4.3% (sd = 2.1). the pearson method demonstrated a low correlation coefficient between mvd and ea (r = 0.429). no relationship between mvd and ea was observed with regard to relapse-free interval and overall survival. conclusion: the histological analysis of angiogenesis expression in patients with colorectal adenocarcinoma can be performed either by computer-assisted image analysis of endothelial area or by conventional microvessels counting. both methods did not show any significant relationship between these angiogenesis parameters with relapse-free interval and overall survival.
Analysis of the correlation between p53 and bcl-2 expression with staging and prognosis of the colorectal adenocarcinoma
Lustosa, Suzana Angelica Silva;Logullo, Angela;Artigiani, Ricardo;Saad, Sarhan Sydney;Goldenberg, Alberto;Matos, Delcio;
Acta Cirurgica Brasileira , 2005, DOI: 10.1590/S0102-86502005000500003
Abstract: purpose: to analyze the correlation between p53 and bcl-2 expression and colorectal adenocarcinoma staging and prognosis. methods: this was a retrospective series of 125 colorectal adenocarcinoma patients (67 women and 58 men; ages 30-87 years) who underwent surgery with curative intent. the mean follow-up was 28.5 months (range: 2-96 months). tnm staging, tumor recurrence, survival and cancer-related mortality were analyzed. immunoreactivity was evaluated using do7 (dako) for p53 and k492 (dako) for bcl-2. tumors with accumulation of staining for cytoplasmic bcl-2 or nuclear p53 in more than 10% of cells were considered positive. statistical analysis utilized pearson chi-squared, log-rank and wilcoxon tests, and kaplan-meier survival estimation (significance level: p<0.05). results: p53+ was found in 11.8% (14/118), bcl-2+ in 50% (58/116) and associated p53+/bcl-2+ in 6.4% (7/109) of the tumors. there was no significant correlation between expression of these biomarkers and tnm i, ii, iii and iv staging (p=0.385 for p53; p=0.461 for bcl-2). for tumor recurrence, p53+ was found in 9.5% (2/21), bcl-2+ in 50% (11/22), and associated p53+/bcl-2+ in 5.2% (1/19) of the tumors (p=0.714, p=1.000 and p=0.960, respectively). for survival analysis, p53+: 57 months (45.0-68.0), bcl-2+: 78 (37.0-89.0), and p53+/bcl-2+: 62 (56.0-68.0) (p=0.319). for cancer-related mortality, p53+: 8.3% (3/36), bcl-2+: 47.2% (17/36), and p53+/bcl-2+: 5.9% (2/36) of the patients (p=0.432, p=0.688 and p=0.907, respectively). conclusion: no correlation was found between tumor expression of p53 and bcl-2 and the tnm staging, recurrence, survival and cancer-related mortality in colorectal adenocarcinoma.
Ki-67 expression score correlates to survival rate in gastrointestinal stromal tumors (GIST)
Artigiani Neto, Ricardo;Logullo, Angela Flavia;Stávale, Jo?o Norberto;Louren?o, Laércio Gomes;
Acta Cirurgica Brasileira , 2012, DOI: 10.1590/S0102-86502012000500007
Abstract: purpose: to evaluate the immunohistochemical expression of p16, ki-67, p53 and bcl-2 proteins in gastrointestinal stromal tumors (gist); to assess the possible association between these variables and clinical and histopathological factors of cancer; and to check for prognostic value of these variables (survival and recurrence). methods: a sample of 55 patients treated surgically for gist in three hospitals was studied. the surgically excised tumors were confirmed as gist by kit, vimentin, desmin s100 protein, cd117, 1a4 and cd34 assessment in paraffin blocks. results: only 9 (16%) cases of gist were positive for p53, p16 was positive among 43.6%; 80% of gists showed staining for bcl-2. the proliferative index (expressed as the proportion of positive cells) assessed by immunohistochemical expression of ki-67 was high in 49% of cases. elevated ki-67 scores were associated to high histological grade (p=0.0026) and mitosis index, mi (p=0.0001). high ki-67 index was associated to death. expression of p53, p16 and bcl-2 did not correlate to morphological or clinical variables. conclusions: ki-67 immunohistochemical evaluation should be included in preoperative evaluation of gist biopsies or surgical specimens as a prognostic tool for clinical staging; and all other proteins studied (bcl-2, p53 and p16) did not play a role in gist metabolic or carcinogenic process, remaining without prognostic value.
Factors affecting compliance with the measles vaccination schedule in a Brazilian city
Logullo, Patricia;Carvalho, Heráclito Barbosa de;Saconi, Renata;Massad, Eduardo;
Sao Paulo Medical Journal , 2008, DOI: 10.1590/S1516-31802008000300006
Abstract: context and objective: the success of vaccination campaigns depends on the degree of adherence to immunization initiatives and schedules. risk factors associated with children's failure to receive the measles vaccine at the correct age were studied in the city of s?o paulo, brazil. design and setting: case-control and exploratory study, in the metropolitan area of s?o paulo. methods: the caregivers of 122 children were interviewed regarding their perceptions and understanding about the measles vaccination and the disease. results: the results showed that age, region of residence, marital status and education level were unrelated to taking measles vaccines adequately. most individuals remembered being informed about the last annual vaccination campaign by television, but no communication channel was significantly associated with vaccination status. the answers to questions about knowledge of the disease or the vaccine, when analyzed alone, were not associated with taking measles vaccinations at the time indicated by health agencies. the results showed that, when parents felt sorry for their children who were going to receive shots, they delayed the vaccination. most of the children did not take the measles vaccination on the exactly recommended date, but delayed or anticipated the shots. conclusion: it is clear that there is no compliance with the government's recommended measles vaccination schedule (i.e. first dose at nine and second at 15 months of age, as recommended in 1999 and 2000). feeling sorry for the children receiving shots can delay vaccination taking.
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