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Search Results: 1 - 10 of 325334 matches for " Ranjna S. Cheema "
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Relationship of Naturally Occurring Antisperm Antibodies in Blood Serum and Seminal Plasma of Cattle Bulls with Sperm Function and Fertility Tests  [PDF]
V. Zodinsanga, Ranjna S. Cheema, P. S. Mavi
Open Journal of Animal Sciences (OJAS) , 2015, DOI: 10.4236/ojas.2015.52014
Abstract: The study was planned with an objective to assess the level of antisperm antibodies (ASA) in the blood serum and seminal plasma of breeding cow bulls and their relationship with sperm function and fertility tests. ASA was analyzed in blood serum and seminal plasma by SpermMar test, Immuno peroxidase assay (IPA) and Enzyme linked immunoabsorbant assay (ELISA). In SpermMar test, about 54% bulls were with >40% IgG in blood serum against sperm surface antigens, whereas none of the bulls were with >10% IgG in seminal plasma. More than 20% and >10% IgA against sperm surface antigens were detected in the blood serum and seminal plasma of 65.8% and 37% bulls, respectively. Out of 26 bulls, seminal plasma of 21 bulls reacted with spermatozoa both in IPA and IgA latex particles and that of only 12 bulls reacted with IgG. In IPA, about 50% of the bulls had >40% ASA against head surface antigens, whereas, there were 23% bulls with >10% ASA in seminal plasma. Also ELISA indicated a higher antibody titre in blood serum (3200 - 6400) and seminal plasma (40 - 80) of 50% and 42% bulls, respectively. There were 11 bulls with low values of HOST/in vitro acrosome reaction/cervical mucus penetration assay and higher level of either serum or seminal plasma ASA. Our study revealed that a significant level of ASA in serum or seminal plasma may have effect on the fertility of bulls by affecting the sperm function.
Immuno-contraceptive potential of sperm specific LDHC4 and SPAM-1 (PH-20) sub units in dog  [PDF]
Ranjna S. Cheema, Nisha Vashishat, Amrit K. Bansal, Gaurav Bakhri, Vinod Kumar Gandotra
Open Journal of Animal Sciences (OJAS) , 2012, DOI: 10.4236/ojas.2012.24037
Abstract: PH-20 (Spam-1) antigen appears to be a bi-functional sperm plasma membrane protein with hyaluronidase activity and its role is related to cumulus penetration as well as zona binding. (LDH-C4) is a key enzyme, distributed specifically in testis and is highly immunogenic, function of which relates to energy metabolism and capacitation of the sperm. Therefore, in this study, we observed the effect of purified native PH-20 and LDH-C4 antigens on sperm function and morphology in dog. Native PH-20 (46, 32 kDa) and LDH-C4 (36, 30, 28) sub units, purified from dog sperm SDS- extracts were used to test as immunocontraceptive candidates in stray dog. Dogs 1, 2, 3, 4, 5/6 and 7/8 were immunized with 46, 32, 36, 30/28, 46/32, 36/30/28 kDa sub units respectively by i/m route and semen was analysed at weekly intervals. High titre of 3200 - 6400 was achieved in all dogs after three boosters. Immune response in treated dogs was confirmed by increase in Ig level in blood serum and reaction of partially purified serum Ig of immunized dogs with respective antigens in double immune diffusion and immunoblots. Immunization of dogs either with individual PH-20/ LDHC sub units or pooled PH-20/LDHC4 sub units showed a significant effect on sperm count, percent motility, viability, HOST and sperm morphology. All the sperm parameters declined to a significant level (p < 0.05) between 45 - 115 DPI and remained low than that of the recommended values for fertile dog semen till 175 DPI. The incubation of spermatozoa in HOS solution in the presence of anti-PH-20 and anti-LDHC Ig significantly reduced the percent HOS positive spermatozoa. A significant decline (p ≤ 0.05) in acrylamide penetration assay was also observed in the presence of anti 46 and 30/28 kDa Ig. Since percent motility, sperm count, HOST and percent normal spermatozoa in immunized dogs were significantly less than that of recommended fertile dog semen, it can be concluded that PH-20 (46/32 kDa) and LDHC4 (36/30/28 kDa) sub units had an immunocontraceptive potential in dog.
Manganese Provides Antioxidant Protection for Sperm Cryopreservation that May Offer New Consideration for Clinical Fertility
Ranjna S. Cheema,Amrit K. Bansal,Gurmail Singh Bilaspuri
Oxidative Medicine and Cellular Longevity , 2009, DOI: 10.4161/oxim.2.3.8804
Abstract: Reactive oxygen species (ROS) are generated by sperm metabolism. While, ROS are required for maturation, capacitation and acrosome reaction, they also modify many peroxidable cellular compounds. There is production of ROS during cryopreservation and frozen spermatozoa are highly sensitive to lipid peroxidation (LPO). Antioxidants exert a protective effect on the plasma membrane of frozen bovine sperm preserving both metabolic activity and cellular viability. Manganese (Mn
Antioxidant effect of Mn2+ on capacitation and acrosome reaction of fresh and chilled cattle bull semen
Amrit Kaur Bansal,Ranjna Sundhey Cheema,Vinod Kumar Gandotra
Veterinary Science Development , 2011, DOI: 10.4081/vsd.2011.e18
Abstract: The aim of this paper was to investigate the antioxidant effect of Mn2+ (200 mM) on the sperm capacitation and acrosome reaction of fresh and chilled cattle bull semen. It has been found that Mn2+ supplementation improves (P≤0.05) the motility at 0, 2, 4 and 6 h of incubation. MDA (malondialdehyde), end product of lipid peroxidation, decreases significantly (P≤0.05) with the supplementation of manganese at 0- and 6-hr of incubation both in fresh and chilled semen. Manganese also increases acrosome reaction significantly (P≤0.05) both in fresh and chilled semen at 0, 4 and 6 h of incubation. Therefore, our findings suggest the role of Mn2+supplementation in improving the quality of cattle bull semen by its scavenging property i.e. reduction in the production of reactive oxygen species during its storage at 4°C or incubation at 37°C for capacitation.
Gross Morphology and Localization of Adenohypophyseal Cells in Camel (Camelus dromedarius) Using A New Combination of Stains
S. A. S. Jaspal, Z. U. Rahman* and A. M. Cheema
Pakistan Veterinary Journal , 2011,
Abstract: Thirty normal camels (Camelus dromedarius) were selected for gross morphological and modified staining of anterior pituitary. Camels were divided in three age groups viz 2-4, 5-10 and above 10 years. Pituitary weight, length, width and circumference were recorded before preservation and at midsegittal cutting. Pituitary weight increased significantly as these animals grew older. Male had heavier pituitary as compared to female. Higher pituitary weight was observed in old as compared to young camel. Sections (4 m) of camel pituitary gland were stained with “Phosphotungstic acid haematoxylin-Orange G-Acid fuchsin-Light green” combination of dyes. This combination of acidic and basic dyes showed affinity to their respective adenohypophyseal cells and proved a suitable combination for differentiation of adenohypophyseal cells and architectural pattern of pituitary gland. Use of Lugol’s Iodine and sodium thiosulphate solution caused mercury fixation which ultimately enhanced the staining of camel adenohypophysis. The whole pituitary presented a brilliant appearance of clarity, enabling cell counts to be performed easily, purely with reference to the colors of adenohypophyseal cell types. This method can be applied for differential staining of adenohypophysis and with good cytology results to the hypophysis of many mammals. The method also provides a sharp contrast between cellular and connective tissue components. With this staining technique, the quantitative and qualitative characteristics of different adenohypophyseal cell types at various functional and hormonal stages, under certain physiological and pathological conditions can also be studied.
Response of Cotton Genotypes to Time of Sowing
M. Akhtar,M. S. Cheema,Moazzam Jamil,Shaukat Ali Shahid
Asian Journal of Plant Sciences , 2002,
Abstract: Study was conducted to evaluate the appropriate sowing time of cotton varieties/cultivars under Bahawalpur conditions. The experiment included four sowing dates started from Ist may up to 16th June, with equal intervals of fifteen days and six varieties viz., V.SLS-1, V.FVH-53, V.BH-118, V.CIM-443, V.CIM-448 with NIAB-Karishma as standard one. Regardless of the varieties/cultivars, the best results were obtained when crop was sown on 16th may. However, cotton varieties NIAB Karishma and BH-118 gave significantly higher weights in a wide range of sowing peirod i.e., from 1st May to 1st June. The highest mean seed cotton yield of 2403 kg ha-1 was obtained when crop was sown on (D2)16th may. Sowing of cotton crop befor or after this sowing date decreased yield of seed cotton significantly.
Effect of Plant Density on Four Short Statured Cotton Varieties
M. Akhtar,M. S. Cheema,Moazzam Jamil,M. Rashad Farooq
Asian Journal of Plant Sciences , 2002,
Abstract: The experiment was conducted to evaluate the maximum yield potential of cotton as effected by plant population for four short statured varieties viz., NIAB-Karishma, NIAB-78, CIM-443 and CIM-448 planted at spacings 10, 20, and 30 cm with rows at 75 cm apart. Maximum seed cotton yield 2657 kg ha -1 was recorded when plant spacing was 30 cm for all the varieties closely followed by 2640 kg ha -1 for the treatment, where plant spacing was 20 cm. The minimum values were obtained at plant spacing of 10 cm.
Sowing Date Cum Varietal Trial on Wheat
M. Akhtar,M. S. Cheema,Liaquat Ali,Moazzam Jamil
Asian Journal of Plant Sciences , 2002,
Abstract: The study was conducted to evaluate the appropriate sowing time of newly evolved wheat varieties/cultivars under Bahawalpur conditions. The study included six sowing dates started from Ist November up to 15th January of year 1997-98 to 1998-99 with equal intervals of fifteen days and seven varieties/cultivars viz; Punjab 96, 95-IB-4173, 7061, MH.97, 92128, 92145 with Inqlab-91 as standard one. Regardless of the varieties /cultivars the best results were obtained when wheat was sown after 15th and before 30th November of year 1997 to 1998. However wheat varieties/cultivars Inqlab – 91 and V. 7061 gave significantly higher weights in a wide range of sowing period i.e; 15th November to 15th December. The highest mean grain yield of 4255 kg ha -1 was obtained when crop was sown on 15th November (D2) followed by 4212.84 kg ha -1 for sowing on 30th November (D3).
Effect of Seed Rate and NPK Fertilizer on Growth and Yield of Wheat Variety Punjnad-1
M.S. Cheema,M. Akhtar,Liaquat Ali
Journal of Agronomy , 2003,
Abstract: The study was conducted for two consecutive crop seasons at Agronomic Research Station, Bahawalpur during Rabi 2000-2001 to 2001-2002 to evaluate maximum yield potential of wheat variety punjnad-1 as affected by various seeding rates 75, 100, 125 and 150 kg ha-1 and NPK levels 0-0-0, 100-50-0, 150-100-50 and 200-100-50 kg ha-1. Maximum wheat grain yield of 4293 kg ha-1 was recorded with fertilizer, level of 150-100-50 NPK kg ha-1 with seeding rate of 125 kg ha-1. It was closely followed by same fertilizer level with seeding rate of 100 kg ha-1 by giving 4287 kg grain yield ha-1 which showed that equally good results were obtained with fertilizer dose of 150-100-50 kg ha-1. It showed that higher dose of NPK fertilizer induce more tillering and grain weight in wheat plants under low seed rate which compansate reduction in grin yield. In both seasons increasing fertilizer levels NPK upto 150-100-50 kg ha-1 with seeding rate of 75, 100 and 125 kg ha-1 significantly and linearly enhance wheat grain yield while reduction in yield was obtained beyond this fertilizer dose and seeding rate.
Response of Wheat Varieties to Sowing Dates
M. Aslam,Manzoor Hussain,M. Akhtar,M.S. Cheema
Journal of Agronomy , 2003,
Abstract: The study was conducted to evaluate the appropriate sowing time of newly evolved wheat varieties/cultivars under Bahawalpur conditions. The study included 6 sowing dates started form Ist November up to 15th January of year 1999-2000 to 2000-2001 with equal intervals of fifteen days and five varieties/cultivars viz., 2236, 2098, 2219 punjnad-1 with inqlab, 91 as standard one. Regardless of varieties/cultivars the best results were obtained when wheat was sown after Ist and before 15th November of year 1999 to 2000. However wheat variety punjnad-1 gave significantly higher weights in a wide range of sowing period i.e., Ist to 30th November. The highest mean grain yield of 5315 kg ha-1 was obtained when crop was sown on 15th November (D2) followed by 5268 kg ha-1 for sowing on Ist November (D1).
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