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Search Results: 1 - 10 of 213303 matches for " Raisa; Qui?ones Pérez "
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Evaluación del medio cromogénico CromoCen? ENT para el diagnóstico clínico de Enterococcus
Díaz Pérez,Marilyn; Iglesias Torrens,Yaidelys; Zhurbenko,Raisa; Quiones Pérez,Dianelys;
Revista Cubana de Medicina Tropical , 2012,
Abstract: introduction: the frequent incidence of enterococci at hospitals and their growing antimicrobial resistance worldwide make the in-hospital surveillance and control a pressing need; consequently, it is indispensable to avail of more sensitive and accurate diagnostic means. objective: to broaden the evaluation of functionality of cromocen? ent chromogenic medium for the isolation and identification of enterococcus spp. from clinical samples. methods: one hundred and fifty clinical samples were analyzed (urine, blood, feces, vaginal smears, skin lesion exudates and exudates from catheters) in the january-april period, 2010 by using the chromogenic medium and the corresponding conventional culture media as controls; the incidence of enterococcus spp was evaluated. the isolations were identified with 12 biochemical tests. from the biochemical identification data, it was possible to determine the quality indicators for both cromocen? ent and the reference media. results: the chromogenic medium encouraged the growth of enterococcus species in 24 hours, allowing their easy recognition due to the pink coloration of the colonies. the diagnostic quality indicator values were over 95 %. the highest percentage of isolates was observed in the urine samples. enterococcus faecalis was the mostly found species. conclusions: cromocen? ent allowed quick and accurate identification of enterococcus spp. from various clinical samples.
Evaluación del medio cromogénico CromoCen ENT para el diagnóstico clínico de Enterococcus Assessment of the CromoCen ENT chromogenic medium for clinical diagnosis of Enterococcus species
Marilyn Díaz Pérez,Yaidelys Iglesias Torrens,Raisa Zhurbenko,Dianelys Quiones Pérez
Revista Cubana de Medicina Tropical , 2012,
Abstract: Introducción: la frecuente incidencia de Enterococcus en los hospitales y su creciente resistencia antimicrobiana a nivel mundial, ha incrementado la necesidad de su vigilancia y control intrahospitalario, por lo que resulta imprescindible contar con medios diagnósticos más sensibles y exactos. Objetivo: ampliar la evaluación de la funcionalidad del medio cromogénico CromoCen ENT para el aislamiento e identificación de Enterococcus spp. procedentes de muestras clínicas. Métodos: se analizaron 150 muestras clínicas (orina, sangre, fecales, exudados vaginales, exudados de lesiones de piel y de catéteres) desde enero hasta abril de 2010, empleando el medio cromogénico y los medios convencionales correspondientes como controles, se evaluó la incidencia de Enterococcus spp. Se identificaron los aislamientos con un conjunto de 12 pruebas bioquímicas. A partir de los datos de la identificación bioquímica se determinaron los indicadores de calidad tanto para el medio CromoCen ENT como para los medios de referencia. Resultados: el medio cromogénico promovió el crecimiento de Enterococcus spp. en solo 24 h, lo cual permitió su fácil reconocimiento por la coloración rosada de las colonias. Los indicadores de calidad diagnóstico mostraron valores superiores a 95 %. El mayor porcentaje de aislamientos se obtuvo en las muestras de orina. Enterococcus faecalis resultó la especie mayormente encontrada en el total de las muestras. Conclusiones: CromoCen ENT permitió la correcta y rápida identificación de Enterococcus spp. procedentes de diversas muestras clínicas. Introduction: the frequent incidence of Enterococci at hospitals and their growing antimicrobial resistance worldwide make the in-hospital surveillance and control a pressing need; consequently, it is indispensable to avail of more sensitive and accurate diagnostic means. Objective: to broaden the evaluation of functionality of CromoCen ENT chromogenic medium for the isolation and identification of Enterococcus spp. from clinical samples. Methods: one hundred and fifty clinical samples were analyzed (urine, blood, feces, vaginal smears, skin lesion exudates and exudates from catheters) in the January-April period, 2010 by using the chromogenic medium and the corresponding conventional culture media as controls; the incidence of Enterococcus spp was evaluated. The isolations were identified with 12 biochemical tests. From the biochemical identification data, it was possible to determine the quality indicators for both CromoCen ENT and the reference media. Results: the chromogenic medium encouraged the grow
La caries dental como urgencia y su asociación con algunos factores de riesgo en ni?os
Pérez Quiones,José Alberto; Mayor Hernández,Félix; Pérez Padrón,Alejandro;
Revista M??dica Electr?3nica , 2010,
Abstract: the disorders produced by dental caries and other oral affections are multiple, making the patient assist to the urgency service. the purpose of this work was evaluating the relations among the sreptococcus mutans and lactobacillus and some demographic variables with dental caries in children, registered in five medical consulting rooms of contreras area, municipality and province of matanzas, for which we carried out an analytic study of the cases kind and controls. the sample was obtained by means of a polistaged, stratified, probabilistic design, and was formed by 900 6-12 years-old children (300 cases and 600 controls). there was a predominance of 6- 8 years-old children both in the cases group and in the control group, and there were not significant differences between the groups. there was prevalence of female children in controls and of male in cases. children from the cases group had a high infection level by streptococcus mutans and lactobacillus, and there was stated a positive association of dental caries with these variables. we designed an educative strategy to evaluate and increase the information level studied children had on this disease.
La caries dental como urgencia y su asociación con algunos factores de riesgo en ni os Dental caries as urgency and its association with some risk facts in children
José Alberto Pérez Quiones,Félix Mayor Hernández,Alejandro Pérez Padrón
Revista M??dica Electr?3nica , 2010,
Abstract: Las dolencias producidas por la caries dental y otras afecciones bucales son múltiples, lo que hace al paciente acudir a los servicios de urgencia. El propósito de este trabajo fue evaluar la relación del estreptococo mutans y lactobacilo y algunas variables demográficas con la caries dental en ni os, pertenecientes a cinco consultorios del área de Contreras, del municipio de Matanzas, para lo cual se realizó un estudio analítico del tipo de casos y controles. La muestra se obtuvo mediante un dise o muestral probabilístico estratificado polietápico y quedó conformada por 900 ni os (300 casos y 600 controles) de 6 a 12 a os de edad. Hubo un predominio de ni os en las edades de 6 a 8 a os, tanto en los casos como en el grupo control, y no se observaron diferencias significativas entre ambos grupos. El sexo femenino prevaleció en los controles, y el masculino, en los casos. Los ni os pertenecientes al grupo de casos, tenían un alto grado de infección por estreptococo mutans y lactobacilos, y se demostró asociación positiva de la caries dental con estas variables. Se dise ó una estrategia educativa para evaluar y elevar el nivel de información que sobre esta enfermedad tenían los ni os objetosde estudio. The disorders produced by dental caries and other oral affections are multiple, making the patient assist to the urgency service. The purpose of this work was evaluating the relations among the sreptococcus mutans and lactobacillus and some demographic variables with dental caries in children, registered in five medical consulting rooms of Contreras area, municipality and province of Matanzas, for which we carried out an analytic study of the cases kind and controls. The sample was obtained by means of a polistaged, stratified, probabilistic design, and was formed by 900 6-12 years-old children (300 cases and 600 controls). There was a predominance of 6- 8 years-old children both in the cases group and in the control group, and there were not significant differences between the groups. There was prevalence of female children in controls and of male in cases. Children from the cases group had a high infection level by streptococcus mutans and lactobacillus, and there was stated a positive association of dental caries with these variables. We designed an educative strategy to evaluate and increase the information level studied children had on this disease.
Asociación del Estreptococos mutans y lactobacilos con la caries dental en ni?os
Pérez Quiones,José Alberto; Duque de Estrada Riverón,Johany; Hidalgo Gato- Fuentes,Iliana;
Revista Cubana de Estomatolog?-a , 2007,
Abstract: dental caries is a disease widely spread in the world, therefore, it has been and it is still the most frequent disease of modern man. due to the high incidence of this oral disease, and to the great need to prevent it, it was decided to conduct this investigation in order to evaluate the connection of the cariogenic microorganisms and some demographic variables with dental caries in children. a case-control analytical study was undertaken in the municipality of matanzas, province of matanzas, during the academic course 2004-2005. the study group was composed of 11 311 children aged 6-12. a randomized sample of 900 children (300 cases and 600 controls) was selected. the highest percentage of white children (62 %) were affected by caries, 91.3 % and 40 % of the children from the case group had an elevated degree of infection caused by streptococcus mutans y lactobacilli, respectively, which showed a positive association between dental caries and these variables.
La caries dental: Algunos de los factores relacionados con su formación en ni?os
Hidalgo Gato- Fuentes,Iliana; Duque de Estrada Riverón,Johany; Pérez Quiones,José Alberto;
Revista Cubana de Estomatolog?-a , 2008,
Abstract: the knowledge of the factors related to the appearance of dental caries is necessary due to the fact that it is a common health problem in the population of all ages. a case-control study was conducted in matanzas during the academic course 2004-2005, aimed at determining the association of some of these risk factors with the appearance of dental caries. the study group was composed of 11 311 children aged 6-12. a sample of 900 was selected from them by a polystage sample design. the high capacity of resistance to the acid attack in the control group (57.2 %), compared with that of the cases (20.3 %), was underlined. the positive association of dental caries with the enamel resistance, its remineralization capacity, as well as with the white lesions and the enamel abnormalities, was established.
Asociación del Estreptococos mutans y lactobacilos con la caries dental en ni os Association of Streptococcus mutans and lactobacilli with dental caries in children
José Alberto Pérez Quiones,Johany Duque de Estrada Riverón,Iliana Hidalgo Gato- Fuentes
Revista Cubana de Estomatolog?-a , 2007,
Abstract: La caries dental es una enfermedad ampliamente extendida en el mundo, por lo que ha sido y sigue siendo la enfermedad más frecuente del hombre moderno. Dada la alta incidencia que presenta esta enfermedad bucal, así como la gran necesidad de prevenirla, decidimos realizar esta investigación, con el objetivo de evaluar la relación de los microorganismos cariogénicos y algunas variables demográficas con la caries dental en ni os. Se realizó un estudio analítico del tipo de casos y controles en el municipio de Matanzas, provincia de Matanzas, en el período comprendido por el curso académico 2004-2005. El universo estuvo constituido por 11 311 ni os entre 6 y 12 a os de edad, determinándose una muestra aleatoria de 900 ni os (300 casos y 600 controles). Se obtuvo que el mayor porcentaje (62 %) de ni os de piel blanca estaba afectados por caries, el 91,3 % y el 40 % de los ni os pertenecientes al grupo de casos, tenían un alto grado de infección por Estreptococo mutans y lactobacilos, respectivamente, lo que demuestra una asociación positiva entre la caries dental y estas variables. Dental caries is a disease widely spread in the world, therefore, it has been and it is still the most frequent disease of modern man. Due to the high incidence of this oral disease, and to the great need to prevent it, it was decided to conduct this investigation in order to evaluate the connection of the cariogenic microorganisms and some demographic variables with dental caries in children. A case-control analytical study was undertaken in the municipality of Matanzas, province of Matanzas, during the academic course 2004-2005. The study group was composed of 11 311 children aged 6-12. A randomized sample of 900 children (300 cases and 600 controls) was selected. The highest percentage of white children (62 %) were affected by caries, 91.3 % and 40 % of the children from the case group had an elevated degree of infection caused by Streptococcus mutans y lactobacilli, respectively, which showed a positive association between dental caries and these variables.
La caries dental: Algunos de los factores relacionados con su formación en ni os Dental caries: Some factors related to their appearance in children
Iliana Hidalgo Gato- Fuentes,Johany Duque de Estrada Riverón,José Alberto Pérez Quiones
Revista Cubana de Estomatolog?-a , 2008,
Abstract: El conocimiento de los factores relacionados con la formación de la caries dental resulta necesario debido a lo extendido que se observa este problema de salud entre la población de todas las edades. Se efectuó un estudio del tipo casos y controles en el municipio de Matanzas, en el período comprendido en el curso académico 2004-2005, con el objetivo de determinar la asociación de algunos de estos factores de riesgo con la aparición de la caries dental. El universo estuvo integrado por 11 311 ni os comprendidos entre las edades de 6 y 12 a os, del cual se extrajo una muestra de 900 ni os mediante un dise o muestral polietápico. Se destacó la alta capacidad de resistencia al ataque ácido en el grupo control (57,2 %) con respecto al de casos (20,3 %), y se estableció la asociación positiva de la caries dental con la resistencia del esmalte, su capacidad de remineralización, así como con las lesiones blancas y las anomalías del esmalte. The knowledge of the factors related to the appearance of dental caries is necessary due to the fact that it is a common health problem in the population of all ages. A case-control study was conducted in Matanzas during the academic course 2004-2005, aimed at determining the association of some of these risk factors with the appearance of dental caries. The study group was composed of 11 311 children aged 6-12. A sample of 900 was selected from them by a polystage sample design. The high capacity of resistance to the acid attack in the control group (57.2 %), compared with that of the cases (20.3 %), was underlined. The positive association of dental caries with the enamel resistance, its remineralization capacity, as well as with the white lesions and the enamel abnormalities, was established.
Susceptibilidad antimicrobiana y factores de virulencia en especies de Enterococcus causantes de infecciones pediátricas en Cuba Antimicrobial susceptibility and virulence factors found in Enterococcus species causing pediatric infections in Cuba
Dianelys Quiones Pérez,Deisy Marrero,Bárbara Falero,Isis Tamargo
Revista Cubana de Medicina Tropical , 2008,
Abstract: Objetivos: conocer la prevalencia de especies de enterococos causantes de infecciones en ni o, su virulencia, susceptibilidad antimicrobiana e infecciones más frecuentes. Métodos: se estudiaron 156 aislamientos de enterococos causantes de infecciones en ni os ingresados en el Hospital "Octavio de la Concepción y La Pedraja", Holguín. Se llevó a cabo la identificación de especies y detección de factores de virulencia por métodos fenotípicos. Se determinó la concentración inhibitoria mínima para vancomicina, ciprofloxacina, ampicilina, gentamicina, estreptomicina, cloranfenicol, nitrofurantoína, eritromicina, tetraciclina, levofloxacina y norfloxacina, según las normas del Clinical Laboratory Standard Institute. Resultados: las cepas se aislaron de sangre, orina, heridas quirúrgicas, piel y tejido blando, oído medio, vulva, punta de catéter, líquido peritoneal, vagina, esperma, líquido cefalorraquídeo, y otras fuentes. La especie prevalente fue Enterococcus faecalis, seguido por Enterococcus faecium, Enterococcus gallinarum y Enterococcus raffinossus, Enterococcus casseliflavus y Enterococcus avium. La producción de proteasa y hemolisina se detectó solo en E. faecalis. E. faecium mostró porcentajes más altos de resistencia a ampicilina, ciprofloxacina, norfloxacina, levofloxacina, mientras que E. faecalis mostró más resistencia a eritromicina, cloranfenicol y tetraciclina. De E. faecalis 58 aislamientos y 12 de E. faecium presentaron altos niveles de resistencia a gentamicina, así como 45 y 32,3 % mostraron altos niveles de resistencia para estreptomicina, respectivamente. Se detectó una cepa de E. faecium resistente a vancomicina. Conclusiones: la circulación de cepas de enterococos virulentas y resistentes a diferentes antibióticos indica la necesidad del control de la infección enterocócica y la importancia del monitoreo de la susceptibilidad antimicrobiana, para prevenir la selección de cepas con estos rasgos dentro de las unidades hospitalarias y su posible expansión a la comunidad. Objectives: to find out the prevalence of Enterococcus species causing child infections, their virulence, antimicrobial susceptibility and most frequent infections. Methods: One hundred and fifty six isolations from Enterococcus causing infections in children, admitted to "Octavio de la Concepción y La Pedraja" hospital in Holguin, were studied. The phenotypical methods allowed identifying species and detecting virulence factors. Following Clinical Laboratory Standard Institute requirements, minimal inhibitory concentration was determined for vancomycin, ciprofloxacin, amp
THE COMPETENCE EVALUATION IN THE BACHELOR IN EDUCATION FORMATION. A PROPOSAL FOR PRIMARY EDUCATION / LA EVALUACIóN POR COMPETENCIAS EN LA FORMACIóN PEDAGóGICA SUPERIOR. UNA PROPUESTA PARA LA CARRERA DE EDUCACIóN PRIMARIA
Danilo A. Quiones Reyna,Zenaida ávila Pérez,Iris Rodríguez Zaldívar
Didasc@lia : Didáctica y Educación , 2011,
Abstract: In the formation of bachelors in education it is necessary that students and teachers get involved in the process directed to contrasting the purposes against the results and the comprehension mixed of what is expected (know how) and what could be defined as level of achievements, in correspondence to the aims established. In this work the procedures and the experiences obtained are exposed in a process of follow up to the evaluation by components in the pedagogical formation. Besides, it is determined as a problematic to be treated though the postgraduate formation in the mastership in education.
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