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Search Results: 1 - 10 of 3214 matches for " Rahasya Mani Mishra "
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Homozygous hemoglobin D with alpha thalassemia: case report
Sanjay Pandey,Rahasya Mani Mishra,Sweta Pandey,Renu Saxena
Open Journal of Hematology , 2011,
Abstract: Hb D is a clinically silent condition, but co-inheritance of Hb D with sickle cell or thalassemia produces clinically significant conditions like sickle cell anemia or thalassemia intermedia and chronic hemolytic anemia of moderate severity. Here we present a case of homozygous Hb D with alpha 3.7kb deletion and phenotypic effect on patients. Diagnosis of Hb D patient was performed by high performance liquid chromatography (HPLC) and complete blood count was measured by automated cell analyzer. Molecular study for common alpha deletions done by Gap-PCR. A homozygous Hb D patient with alpha thalassemia was present mild clinical manifestations with normal reticulocytes and red cell indices. Thus observed case conclude the co-existence of alpha 3.7 deletions with homozygous Hb D present mild clinical – hematological picture.
Sanjay Pandey,Sweta Pandey,Rahasya Mani Mishra,Renu Saxena
Mediterranean Journal of Hematology and Infectious Diseases , 2012, DOI: 10.4084/mjhid.2012.
Abstract: Xmn-1 polymorphism is a known factor, which increases fetal haemoglobin production. Among the inherited disorders of blood, thalassaemia and SCD constitutes a major bulk of genetic diseases in India. Our aim was to verify the role of the Xmn I polymorphism as a modulating factor in sickle cell patients and frequency of the polymorphism in Indian sickle cell patients. Subjects were 60 sickles homozygous and 75 sickle beta thalassemia patients. 5 ml blood samples collected from patients. Screening of sickle patients done by HPLC. An automated cell analyzer SYSMEX (K-4500 Model) used to analyze the CBC of patients.Xmn1 polymorphism analysis done by PCR-RFLP and Statistical analysis was performed on GraphPad static’s software. t test applied to compare the means amongst group. Among the sickle homozygous 27 were heterozygous (+/-) and 19 were homozygous (+/+) while 30 were heterozygous and 24 were homozygous in sickle β-thalassemia patients. Extremely significant differences (p-value <0.001) of hematological parameters seen among patient with xmn-1 carrier and without the xmn-1 carrier. In our cases the clinical symptom less frequent and higher HbF level with Xmn-1 carriers. Presence of Xmn-1 polymorphism in sickle patients with higher HbF that improve phenotypic presentation in the sickle cell patients. We conclude that the phenotype of Indian sickle cell patients influenced by Xmn-1 polymorphism.
Prevalence of glutathione S-transferase gene deletions and their effect on sickle cell patients
Sanjay, Pandey;Mani, Mishra Rahasya;Sweta, Pandey;Vineet, Shah;Kumar, Ahuja Rajesh;Renu, Saxena;
Revista Brasileira de Hematologia e Hemoterapia , 2012, DOI: 10.5581/1516-8484.20120030
Abstract: background: glutathione s-transferase gene deletions are known detoxification agents and cause oxidative damage. due to the different pathophysiology of anemia in thalassemia and sickle cell disease, there are significant differences in the pathophysiology of iron overload and iron-related complications in these disorders. objective: the aim of this study was to estimate the frequency of the gstm1 and gstt1 genotypes in sickle cell disease patients and their effect on iron status. methods: forty sickle cell anemia and sixty sickle ?-thalassemia patients and 100 controls were evaluated to determine the frequency of gst gene deletions. complete blood counts were performed by an automated cell analyzer. hemoglobin f, hemoglobin a, hemoglobin a2 and hemoglobin s were measured and diagnosis of patients was achieved by high performance liquid chromatography with dna extraction by the phenol-chloroform method. the gst null genotype was determined using multiplex polymerase chain reaction and serum ferritin was measured using an elisa kit. statistical analysis was by epiinfo and graphpad statistics software. results: an increased frequency of the gstt1 null genotype (p-value = 0.05) was seen in the patients. the mean serum ferritin level was higher in patients with the gst genotypes than in controls; this was statistically significant for all genotypes except gstm1, however the higher levels of serum ferritin were due to blood transfusions in patients. conclusion: gst deletions do not play a direct role in iron overload of sickle cell patients.
Design and Development of Virtual Objects to be Used with Haptic Device for Motor Rehabilitation  [PDF]
Jatin B. Maniya, Pushparaj Mani Pathak, Bhanu Kumar Mishra
Journal of Software Engineering and Applications (JSEA) , 2010, DOI: 10.4236/jsea.2010.310116
Abstract: This paper presents design and development of virtual objects to be used with haptic device PHANTOM Omni for motor rehabilitation by incorporating visual and haptic feedback. Developed predominantly for augmentation of motor skill of patient, the objects could also be used for teaching and enhancing writing skill of children. The virtual objects include English alphabets, numbers, string and mazes, and games. The scheme has been evaluated on children from 11 to 14 years age. The experiment gives good results.
Cosmic Ray Associated with Coronal Index and Solar Flare Index during Solar Cycle 22 - 23  [PDF]
Prithvi Raj Singh, Shabir Ahmad, A. C. Pandey, Ajay Kumar Saxena, Chandra Mani Tiwari, A. P. Mishra
International Journal of Astronomy and Astrophysics (IJAA) , 2017, DOI: 10.4236/ijaa.2017.73013
Abstract: We study the relation between monthly average counting rates of the cosmic ray intensity (CRI) observed at Moscow Neutron Monitoring Station, solar flare index (SFI) and coronal index during the solar cycles 22 and 23, for the period 1986-2008. The long-term behaviour of various solar activity parameters: sunspot numbers (SSN), solar flare index (Hα flare index), coronal index (CI) in relation to the duration of solar cycles 22 and 23 is examined. We find that the correlation coefficient of CRI with the coronal index as well as Hα flare index is relatively large anti-correlation during solar cycle 22. However, the monthly mean values of sunspot number, Hα flare index, and coronal index are well positively correlated with each other. We have analyzed the statistical analysis of the above parameters using of linear model and second order polynomial fits model.
Culture conditions for the production of a-galactosidase by Aspergillus parasiticus MTCC-2796: a novel source
Shivam,Kumar; Tripathi,Chandra Prakash Mani; Mishra,Sarad Kumar;
Electronic Journal of Biotechnology , 2009,
Abstract: aspergillus parasiticus microbial type culture collection (mtcc)-2796, a new source of a-galactosidase is an efficient producer of enzyme in basic medium under submerged fermentation conditions. maximum a-galactosidase production (156.25 uml-1) was obtained when the basic medium is supplemented with galactose (0.5% w/v) and raffinose (0.5% w/v) as carbon source and yeast extract as nitrogen source. enzyme production was also enhanced considerably in the presence of wheat bran (1.0% w/v). enzyme secretion was strongly inhibited by the presence of hg2+, cu2+, and co2+ in the medium and to some extent by zn2+ and ni2+, while marginal increase in the enzyme production was observed when mg2+ and mn2+ were added in the medium. among amino acids checked (aparagine, cysteine, glutamine, leucine and proline), glutamine (1 mm) was found to be an enhancer for the enzyme production. the temperature and ph range for the production of enzyme were 25oc to 35oc and 6.5 to 7.5, respectively with maximum activity (50 uml-1) at 30oc and ph 6.5 under static fermentation condition.
Molecular tissue typing in renal transplantation: Initial experience from a tertiary care Naval Hospital
Mishra Mahendra,Mani Haresh,Saxena Vinod,Gupta Mahendra
Indian Journal of Human Genetics , 2006,
Abstract: Background:During the last five years the proportion of living unrelated kidney transplants has increased and DNA tissue-typing methods have become popular in India. This study was carried out to compare the results of tissue - typing by serology and sequence specific primers (SSP) and study the usefulness of ′episode′ allograft biopsies for diagnosis of acute graft dysfunction . Materials and Methods:DNA was extracted from whole blood using Qiagen kit. Samples from 60 individuals including thirty patients and their donors were typed by serology and SSP. Fifteen allograft biopsies were performed for suspected acute rejection (AR) cases. Results: Both alleles of HLA - A, B and DR antigen could be determined in 86, 65 and 90% of samples by SSP respectively. There was a discrepancy of 16-40% between SSP and serology. Acute rejection was confirmed in 8/15 biopsies. Graft survival rates were 83 and 76% at one and two years respectively. Neither the graft survival nor the number of AR episodes showed any correlation with the extent of HLA mismatch. SSP was useful in defining AFNx0168, AFNx0166, AFNx0169 and AFNx0133 alleles at private level and AFNx0136, AFNx0174 and AFNx0103 alleles which were blank on serology. Conclusions: SSP has become popular in India due to its simplicity, superior results especially for class II HLA alleles: and episode allograft biopsy is adequate for follow-up of kidney recipients.
Optimization of a divided wall column for the separation of C4-C6 normal paraffin mixture using Box-Behnken design
Sangal Vikas K.,Kumar Vineet,Mishra Mani Indra
Chemical Industry and Chemical Engineering Quarterly , 2013, DOI: 10.2298/ciceq121019047s
Abstract: In the present study, simulation of a divided wall column (DWC) was carried out to study the product quality and energy efficiency as a function of reflux rate, liquid spilt and vapour split for the separation of C4-C6 normal paraffin ternary mixture. Rigorous simulation of the DWC was carried out using Multifrac model of ASPEN Plus software. Box-Behnken design (BBD) was used for the optimization of parameters and to evaluate the effects and interaction of the process parameters such as reflux rate (r), liquid split (l) and vapour split (v). It was found that the number of simulation runs reduced significantly for the optimization of DWC by BBD. Optimization by BBD under response surface methodology (RSM) vividly underscores interactions between variables and their effects. The predictions agree well with the results of the rigorous simulation.
First Principles Calculations to Study the Equilibrium Configuration of Ozone Molecule
Laxman Mainali,Devendra Raj Mishra,Mukunda Mani Aryal
Physics , 2011,
Abstract: The present work describes the equilibrium configuration of the ozone molecule studied using the Hartree-Fock (HF), Moller-Plesset second order (MP2), Configuration interaction (CI), and Density functional theory (DFT) calculations. With the MP2 calculations, the total energy for the singlet state of ozone molecule has been estimated to be -224.97820 a.u., which is lower than that of the triplet state by 2.01 eV and for the singlet state the bond length and the bond angle have been estimated to be 1.282 {\AA} and 116.850 respectively. Calculations have also been performed to obtain the total energy of the isomeric excited state of ozone molecule and it is seen that the ground state energy of the ozone molecule is lower than that of the isomeric excited state by 1.79 eV. Furthermore, the binding energy of the ozone molecule has been estimated to be 586.89 kJ/mol. The bond length, bond angle and the binding energy estimated with HF, MP2, CI and DFT are compared with the previously reported experimental values.
Rapid and accurate detection of Cyanobacterial toxin microcystin-LR using fiber-optic long-period grating based immunosensor
Saurabh Mani Tripathi,Wojtek J. Bock,Predrag Mikulic,Garima Mishra
Physics , 2014,
Abstract: In this paper we present a label-free, stable and highly sensitive fiber-optic sensor for detection of environmental toxin microcystin-LR (MC-LR). Thiolated MC-LR-targeting aptamer covalently immobilized on dual-resonance long-period fiber gratings (DR-LPFG) is used as MC-LR recognition element and the spectral response of the DR-LPFG is monitored for various concentrations of MC-LR. Sensitivity of the DR-LPFG is optimized by coating an appropriate thickness of gold layer to the fiber surface. Gold coating serves two purposes, first it locally supports a surface-Plasmon mode associated with the modified cladding modes and second it helps covalently immobilize the MC-LR targeting aptamer to the sensor surface. Monitoring both the resonance wavelengths of our ultra sensitive DR-LPFGs (3891.5 nm/RIU) we study the aptamer and MC-LR binding to the sensor surface. Real-time, efficient MC-LR molecular binding to the sensor surface is also confirmed using atomic force microscopy. The detection limit of our sensor, limited by the molecule size of MC-LR, its fractional surface coverage of the sensor, and sensor length, is 5 ng/mL, which can be further improved by increasing the fractional MC-LR coverage on the sensor surface by (i) reducing the LPFG length (ii) increasing grating strength, (iii) increasing the LPFG sensitivity by coupling to even higher order cladding modes by reducing the grating period.
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