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Search Results: 1 - 10 of 489576 matches for " Rafael; Martínez Antu?a "
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Diagnóstico de linfomas cutáneos mediante detección de clonalidad por PCR-heterodúplex
Amor Vigil,Ana M.; Matos Borges,Rafael; Martínez Antua,Gisela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2008,
Abstract: the clonicity detection in the lymphoproliferative syndromes by studying the rearrangement of the immunoglobulin genes and of the t-receptor cells is used to make clear if a proliferation or infiltrate of lymphocytes is malignant or not. this type of study is particularly useful in the presence of cutaneous lesions whose lymphoid or dermatological origin is difficult to define. by the pcr-heteroduplex technique, the genes of the immunoglobulin heavy chain and of the t-cell receptor chain were studied in 10 patients that presented dermatological manifestations attributable to some kind of cutaneous lymphoma. clonal rearrangement was observed in 7 patients, which allowed to confirm the diagnosis of mycosis fungoides and other types of cutaneous lymphomas. it was not possible to confirm a lymphoid process of malignant character by this technique in 3 patients who did not show clonal rearrangement. the usefulness of the study was proved when in the presence of a skin affection, it was difficult to differentiate a dermatological process from a proliferative syndrome with cutaneous manifestations.
Diagnóstico de linfomas cutáneos mediante detección de clonalidad por PCR-heterodúplex Diagnosis of cutaneous lymphomas by clonicity detection through PCR-heteroduplex technique
Ana M. Amor Vigil,Rafael Matos Borges,Gisela Martínez Antua
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2008,
Abstract: La detección de clonalidad en los síndromes linfoproliferativos mediante el estudio del reordenamiento de los genes de las inmuglobulinas y del receptor de células T, es utilizada para esclarecer si una proliferación o infiltrado de linfocitos es maligno o no. Este tipo de estudio es de particular utilidad en presencia de lesiones cutáneas cuyo origen linfoide o dermatológico resulta difícil de definir. Mediante la técnica de PCR-heterodúplex se estudiaron los genes de la cadena pesada de las inmunoglobulinas y de la cadena gamma del receptor de las células T, en 10 pacientes que presentaban manifestaciones dermatológicas atribuibles a algún tipo de linfoma cutáneo. Se observó reordenamiento clonal en 7 pacientes, lo cual permitió confirmar el diagnóstico de micosis fungoide y otros tipos de linfomas cutáneos. En 3 pacientes que no mostraron reordenamiento clonal, no fue posible confirmar por esta técnica un proceso linfoide de carácter maligno. Se demostró la utilidad del estudio cuando en presencia de una afección en la piel, es difícil diferenciar un proceso dermatológico de un síndrome linfoproliferativo con manifestaciones en piel. The clonicity detection in the lymphoproliferative syndromes by studying the rearrangement of the immunoglobulin genes and of the T-receptor cells is used to make clear if a proliferation or infiltrate of lymphocytes is malignant or not. This type of study is particularly useful in the presence of cutaneous lesions whose lymphoid or dermatological origin is difficult to define. By the PCR-heteroduplex technique, the genes of the immunoglobulin heavy chain and of the T-cell receptor chain were studied in 10 patients that presented dermatological manifestations attributable to some kind of cutaneous lymphoma. Clonal rearrangement was observed in 7 patients, which allowed to confirm the diagnosis of mycosis fungoides and other types of cutaneous lymphomas. It was not possible to confirm a lymphoid process of malignant character by this technique in 3 patients who did not show clonal rearrangement. The usefulness of the study was proved when in the presence of a skin affection, it was difficult to differentiate a dermatological process from a proliferative syndrome with cutaneous manifestations.
Tratamiento de la leucemia promielocítica con ácido transretinoico y quimioterapia intensiva: Evolución clínica y molecular Treatment of promyelocytic leukemia using transretinoic acid and intensive chemotherapy: Clinical and molecular progress
Porfirio Hernández Ramírez,Gisela Martínez Antua,Edgardo Espinosa Martínez,Rafael Losada Buchillón
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2002,
Abstract: Cuarenta y nueve pacientes con diagnóstico de leucemia promielocítica se trataron con ácido retinoico (45-50 mg/m2/día) durante la inducción, después se hizo consolidación con rubidomicina y arabinósido de citosina, seguida de un matenimiento con 6 mercaptopurina y metotrexate durante 2,5 a os. Se realizó un estudio evolutivo clínico hematológico y molecular. Cuarenta y tres pacientes lograron remisión completa (88 %); 9 (47 %) de 19 enfermos, y 18 (90 %) de 20, se encontraban en remisión molecular después de la inducción y la consolidación, respectivamente. La sobrevida global a los 5 a os fue de 65 % ± 8 % y la sobrevida libre de evento fue 63 % ± 7 %. La sobrevida libre de enfermedad fue de 71 % ± 7 % en igual período Forty nine patients diagnosed with promyelocytic leukemia were treated with retinoic acid (45-50 mg/m2/day) during induction; afterwards, a consolidation therapy was applied with daunorubicin and arabinosylcitosine, followed by a maintenance therapy with 6 mercaptopurine and methotrexate for 2 years and a half. An evolving clinical, hematological and molecular study was performed. Forty three patients achieved complete remission (88%); 9 (47%) of 19 patients and 18(90%) of 20 patients had reached molecular remission after induction and consolidation respectively. Global survival rate at 5 years was 65%±8% and event-free survival rate was 63%±7%. Disease-free survival rate was 71%± 7% in the same period
Bases moleculares de las leucemias agudas
G. Martínez Antua
Iatreia , 2006,
Abstract: El gran desarrollo de la biología molecular en los últimos a os ha contribuido a un importante avance en los conocimientos relacionados con las bases moleculares de las leucemias agudas (LA). Ademas de profundizar en la biología de estas enfermedades y conocer las bases moleculares, ha renido también gran impacto en mejorar el resultado de los tratamientos y disminuir la toxicidad de las terapias.
Tratamiento de la leucemia promielocítica con ácido transretinoico y quimioterapia intensiva: Evolución clínica y molecular
Hernández Ramírez,Porfirio; Martínez Antua,Gisela; Espinosa Martínez,Edgardo; Losada Buchillón,Rafael; González Otero,Alejandro; Machín García,Sergio; Dorticós Balea,Elvira; Milanés Roldán,María Teresa; Cayado,Niubis; Gramatges Ortiz,Anissa; Bravo Regueiro,Jenny; Carnot Uría,José;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2002,
Abstract: forty nine patients diagnosed with promyelocytic leukemia were treated with retinoic acid (45-50 mg/m2/day) during induction; afterwards, a consolidation therapy was applied with daunorubicin and arabinosylcitosine, followed by a maintenance therapy with 6 mercaptopurine and methotrexate for 2 years and a half. an evolving clinical, hematological and molecular study was performed. forty three patients achieved complete remission (88%); 9 (47%) of 19 patients and 18(90%) of 20 patients had reached molecular remission after induction and consolidation respectively. global survival rate at 5 years was 65%±8% and event-free survival rate was 63%±7%. disease-free survival rate was 71%± 7% in the same period
Importancia del estudio del quimerismo en el trasplante alogénico de médula ósea
Amor Vigil,Ana M; Martínez Antua,Gisela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2003,
Abstract: since the hematopoietic transplantation was performed for the first time, the importance of knowing chimerism was underlined. the present paper updates the concepts of chimerism, its classification and the different ways to determine it. the most recommended is the highly polymorphic area amplification in the dna using the polymerase chain reaction technique. this technique has allowed carrying out studies on the evolution of chimera, to relate the set level of chimerism with the graft behaviour and the graft-versus-host disease under different conditioning. it has also made it possible to early detect relapse of transplanted patients and the prompt administration of additional immunotherapy. finally, the paper presents the recommendations of the american society of blood and bone marrow transplantation for standardizing the study of chimerism in the therapy centers
Importancia del estudio del quimerismo en el trasplante alogénico de médula ósea IMPORTANCE OF THE STUDY OF CHIMERISM IN THE ALLOGENEIC BONE MARROW TRANSPLANTATION
Ana M Amor Vigil,Gisela Martínez Antua
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2003,
Abstract: Desde que se comenzó a realizar el trasplante hematopoyético se reconoció la importancia de conocer el establecimiento de quimerismo. El presente trabajo actualiza los conceptos de quimerismo, su clasificación y las diferentes vías para su determinación. Se destaca como la más recomendada, la amplificación de zonas altamente polimórficas en el ADN por la técnica de la reacción en cadena de la polimerasa. La utilización de esta técnica ha permitido realizar estudios de la evolución de la quimera, relacionar el grado de quimerismo establecido con el comportamiento del injerto y de la enfermedad de injerto contra hospedero en los diferentes regímenes de acondicionamiento. También ha posibilitado la detección precoz de la recaída en los pacientes trasplantados y la administración oportuna de inmunoterapia adicional. Finalmente, se presentan las recomendaciones de la Sociedad Americana de Trasplante de Sangre y Médula ósea para estandarizar el estudio del quimerismo en los diferentes centros de tratamiento Since the hematopoietic transplantation was performed for the first time, the importance of knowing chimerism was underlined. The present paper updates the concepts of chimerism, its classification and the different ways to determine it. The most recommended is the highly polymorphic area amplification in the DNA using the polymerase chain reaction technique. This technique has allowed carrying out studies on the evolution of chimera, to relate the set level of chimerism with the graft behaviour and the graft-versus-host disease under different conditioning. It has also made it possible to early detect relapse of transplanted patients and the prompt administration of additional immunotherapy. Finally, the paper presents the recommendations of the American Society of Blood and Bone Marrow Transplantation for standardizing the study of chimerism in the therapy centers
Negotiating Darwin. The Vatican Confronts Evolution (1877-1902)
Rafael A. Martínez
Anuario de Historia de la Iglesia , 2009,
Abstract: A finales del siglo xix la teoría de la evolución encontró una actitud crítica por parte de la Iglesia Católica pero, quizás sorprendentemente, no hubo ninguna condena oficial por parte de las autoridades de la Santa Sede. La documentación de los Archivos del Santo Oficio y del índice han permitido reconstruir la actuación de las Congregaciones Romanas. En este estudio se examina el resultado de esta investigación, pero también sus límites y las perspectivas que puede abrir para una mayor comprensión de las relaciones entre ciencia y fe.
CONSUMO DE TABACO, UNA ENFERMEDAD SOCIAL
CHáVEZ DOMíNGUEZ, RAFAEL CéSAR;LóPEZ ANTU?ANO, FRANCISCO J.;REGALADO PINEDA, JUSTINO;ESPINOSA MARTíNEZ, MARLENE;
Revista del Instituto Nacional de Enfermedades Respiratorias , 2004,
Abstract: organized effort to control tobacco consumption and its malignant effects has not been enough. it is therefore convenient to focus on the strong social disease component it encloses. to stop this disease it must undergo a social maturing process. the dynamics of intervention should be fed back by their results. mortality, morbidity, trends and risks are good indicators of the degree of social involvement. today, there is a body of evidence about the interrelationship between individual behavior and the social environment. also, mexico has formally accepted the framework convention on tobacco control, which will help to increase social pressure. smoking requires individual and social care at all ages starting from elementary school. it is urgent to raise public awareness and encourage the concern for taking care. health professionals, who are the best witnesses of smoking consequences: diseases, miseries and deaths, should be more concerned and give the good example. other professions should also be involved. it is necessary to innovate strategies and continue looking for cost - effective trials. there is a need to modify the present concept with preventive implications such as: adjusting the present concept, considering tobacco consumption a consequence of a deviation of individual and social behavior; taking care of the new social disease risks; improving health and well-being promotion programs in a cultural environment. shared community actions are advantageous. a harmonic environment contributes to reach the goal.
Diagnóstico molecular de la leucemia aguda promielocítica: Resultados preliminares Molecular diagnosis of acute promyelocytic leukemia: preliminary results
Gisela Martínez Antua,Niubys Cayado Gutiérrez,Adriana Mu?iz Fernández,Edgardo Espinosa Martínez
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2000,
Abstract: La leucemia aguda promielocítica (LAP) se caracteriza por la presencia de la translocación recíproca t (15;17) que tiene como resultado la formación del gen híbrido PML-RARa . Como la LAP se considera una emergencia hematológica y además tiene hoy en día un tratamiento muy específico con ácido retinoico (ATRA), es muy importante hacer un diagnóstico rápido y preciso, que en muchos casos permite incluso salvar la vida del paciente. En la actualidad se han desarrollado métodos de RT-PCR para detectar el gen híbrido PML-RARa . Estas técnicas moleculares han sido extremadamente útiles en el diagnóstico de esta entidad. En este trabajo presentamos los resultados preliminares del diagnóstico molecular en 38 pacientes con LAP. En 36 pacientes se demostró la presencia del gen híbrido y 2 fueron negativos. Del total de enfermos con resultados positivos, 19 (55 %) fueron bcr 1, 2 (5 %) fueron bcr 2 y 14 (40 %) fueron bcr 3. Todos los pacientes con resultados positivos respondieron al tratamiento con ATRA. En 1 de los 2 pacientes con resultados negativos se demostró la presencia de la t(11;17). Ninguno de estos 2 enfermos respondió al tratamiento con ATRA Acute promyelocytic leukemia (APL) is characterized by the reciprocal translocation t(15; 17) that results in fusion gene PML-RARa formation. As APL is considered to be an hematological emergency and also is given a very specific treatment with retinoic acid (ATRA), then it is very important to diagnose quickly and accurately which makes it possible in many cases to save the patient's life. At present RT- PCR methods have been developed to detect PML-RARa gen. These molecular techniques have been extremely useful in diagnosing this entity. This paper sets forth the preliminary results of molecular diagnoses of APL in 38 patients. 36 cases presented the fusion gene and 2 did not. Of the total number of positive patients, 19(55%) were bcr 1, 2 (5%) were bcr 2 and 14(40%) bcr 3. All the patients with positive results were responsive to treatment with ATRA. One of the two patients with negative results showed the existence of t (11; 17). These two patients did not respond to treatment with ATRA
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