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Search Results: 1 - 10 of 13698 matches for " Rafael Llanes Caballero "
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Uretritis por Haemophilus influenzae serotipo b: Reporte de un caso
LLANES CABALLERO,RAFAEL; CABRERA CANTELAR,NEREYDA; PALMA MONROY,SARA;
Revista Cubana de Medicina Tropical , 1996,
Abstract: a case of a patient with schistosomiasis haematobium history, from mozambique, is presented. this patient was admitted in the institute of tropical medicine for having urination troubles and purulent urethral secretion. serotype b haemophilus influenzae, biotype iv of the urethra, was isolated. the strain was sensitive to ampicillin, chloramphenicol, ceftraxione, and norfloxacin, and resistant to tetracycline and erythromycin. the patient got better after receiving treatment with norfloxacin. a comment is made on the role of this microorganism as a sexual transmission pathogen.
Uretritis por Haemophilus influenzae serotipo b: Reporte de un caso
RAFAEL LLANES CABALLERO,NEREYDA CABRERA CANTELAR,SARA PALMA MONROY
Revista Cubana de Medicina Tropical , 1996,
Abstract: Se presenta el caso de un paciente con antecedentes de esquistosomiasis haematobium, procedente de Mozambique, que ingresa en el Instituto de Medicina Tropical "Pedro Kourí" por presentar molestias al orinar y secresión uretral purulenta. Se aisló Haemophilus influenzae serotipo b, biotipo IV, de la uretra. La cepa resultó ser sensible a la ampicilina, cloranfenicol, ceftriaxona y norfloxacina y resistente a la tetraciclina y eritromicina. El paciente evolución hacia la curación luego de recibir tratamiento con norfloxacina. Se realiza un comentario sobre el papel de este microorganismo como patógeno de transmisión sexual. A case of a patient with schistosomiasis haematobium history, from Mozambique, is presented. This patient was admitted in the Institute of Tropical Medicine for having urination troubles and purulent urethral secretion. Serotype B Haemophilus influenzae, biotype IV of the urethra, was isolated. The strain was sensitive to ampicillin, chloramphenicol, ceftraxione, and norfloxacin, and resistant to tetracycline and erythromycin. The patient got better after receiving treatment with norfloxacin. A comment is made on the role of this microorganism as a sexual transmission pathogen.
Susceptibilidad antimicrobiana de cepas de Neisseria gonorrhoeae por difusión con discos
Rafael Llanes Caballero,Juan C. Acosta Giraldo,Jorge Sosa Puente,Daymi Guzmán Hernández
Revista Cubana de Medicina Tropical , 1999,
Abstract: Se realizó en el laboratorio de Gonococo del Insituto de Medicina Tropical "Pedro Kourí" un estudio de la susceptibilidad in vitro de Neisseria gonorrhoeae a la penicilina, tetraciclina, cefuroxima, ceftriaxona, cefotaxina y ciprofloxacina, por el método de difusión con discos, utilizando el medio de agar base GC más suplemento. En una primera etapa se estandarizó el método y se utilizó la cepa de referencia de N. gonorrhoeae ATCC 49226. En la segunda etapa se estudiaron 50 cepas de gonococo aisladas de 8 provincias de Cuba durante 1995 y 1996. Los resultados de la estandarización demostraron que los valores de susceptibilidad antimicrobiana estaban dentro de los límites aceptados. El 52 y el 34 % de las cepas fueron resistentes a la penicilina y la tetraciclina respectivamente y todas resultaron susceptibles al resto de los antimicrobianos evaluados. Recomendamos el uso del método de difusión con discos para la vigilancia de la sensibilidad del gonococo a estas drogas en nuestro país. The Gonoccocus Laboratory of "Pedro Kourí" Tropical Medicine Institute carried out a study of in vitro susceptibility of Neisseria gonorrhoeae to penicillin, tetracycline, cefuroxime ceftriaxone, cefotaxine and ciprofoxacin by means of a disk diffusion method with the culture medium agar base GC plus supplement. In the first phase, the method was standardized and the reference N. gonorrhoeae ATCC 49226 strain was used whereas in the second phase, 50 gonococcal strains isolated in 8 provinces during 1995 and 1996 were examined. The results of such standardization confirmed that the antimicrobial susceptibility values were within the allowable limits. 52 and 34 % of strains were resistant to penicillin and tetracycline respectively and all of them showed susceptibility to the rest of evaluated antimicrobial drugs. We recommend the use of the disk diffusion method for surveillance of gonococci resistance to these drugs in our country.
Serotipos de Streptococcus pneumoniae en Cuba y progresión de la resistencia a la penicilina Streptococcus pneumoniae serotypes in Cuba and progression of resistance to penicilina
Gilda T. Tora?o Peraza,Rafael Llanes Caballero,Lisania M. Pías Solis,Miriam Abreu Capote
Revista Cubana de Medicina Tropical , 2010,
Abstract: INTRODUCCIóN: en Cuba, a partir de 2006, ha sido difícil establecer la incidencia real de las meningitis bacterianas y otras enfermedades invasivas por Streptococcus pneumoniae, como consecuencia del bajo porcentaje de aislamiento e identificación de este agente en la red de laboratorios de microbiología del país. OBJETIVO: alertar sobre la necesidad de revitalizar la vigilancia activa de la enfermedad neumocócica invasiva, epidemiológica y microbiológica, fundamentalmente las meningitis y neumonías, en la etapa previa a la vacunación. MéTODOS: se compararon la circulación de los serogrupos/serotipos capsulares y la susceptibilidad a penicilina de los aislamientos de S. pneumoniae responsables de infecciones invasivas, en dos períodos de la vigilancia: a os 1994-2006 y 2007-2009. RESULTADOS: los serogrupos más frecuentes fueron: 3, 6, 7, 9, 14, 17, 18, 19, 20 y 23, pero se constató durante el segundo período de estudio, la circulación de otros no contenidos en las vacunas conjugadas disponibles comercialmente (serogrupo 15) y asociados a resistencia a antimicrobianos (serotipo 5). Por otra parte, se puso de manifiesto un incremento de la resistencia a la penicilina. CONCLUSIONES: para garantizar una alta efectividad con la inclusión en el calendario de vacunación infantil en Cuba, de alguna de las vacunas conjugadas contra S. pneumoniae, se impone el estudio de un número más representativo de aislamientos. Esto también permitirá hacer una correcta evaluación de la tendencia al incremento de la resistencia a la penicilina y adoptar guías de tratamiento adecuadas en el medio cubano. INTRODUCTION: since 2006 in Cuba, there has been difficult to determine the actual incidente of bacterial meningitis and other invasive diseases caused by Streptococcus pneumoniae, as a result of the low percentage of isolation and identification of this agent in the domestic microbiology lab network. OBJECTIVE: to warn of the need of revitalizing the active surveillance of invasive, epidemiological and microbiological pneumoccocic diseases, mainly meningitis and pneumoniae in the phase prior to vaccination. METHODS: a comparison was made on the circulation of capsulary serogroups/serotypes and the invasive infection-causing S. pneumoniae isolates susceptibility to penicilline in two periods: 1994-2006 and 2007-2009. RESULTS: the most frequent serogroups were 3, 6, 7, 9, 14, 17, 18, 19, 20 and 23; however, in the second period, it was observed that others not contained in the available conjugate vaccines (serogrup 15) and associated to antimicrobial resistance (serotype 5) wer
Primer reporte de Neisseria polysaccharea en Cuba
Guzmán Hernández,Daymi; Llanes Caballero,Rafael; Martínez Motas,Isabel; Valdés Ramos,Eduardo A.; Sosa Puente,Jorge;
Revista Cubana de Medicina Tropical , 1999,
Abstract: the national laboratory of reference of pathogenic neisserias of ?pedro kourí? tropical medicine institute studied 58 strains which had been previously identified as neisseria meningiditis and isolated from nasopharyngeal carriers from 1985-1998. they were applied the knapp detection scheme plus the glutamiltransferase production test. the latter and the polyscharide production based on 5 % sacarose allowed to make a differential diagnosis between n. meningitidis and neisseria polyccharea. it was possible to identify a n. polysaccharia strain, a species that is reported for the first time in cuba.
Caracterización de cepas de Haemophilus influenzae mediante fermentación de azúcares
LLANES CABALLERO,RAFAEL; AZAHARES ROMERO,LUIS E.; PéREZ MONRAS,MIRIAM F.; MARTíNEZ DíAZ,ALICIA;
Revista Cubana de Medicina Tropical , 1997,
Abstract: 64 haemophilus influenzae strains circulating in havana city during a year were characterized by the carbohidrate fermentation method for the first time in cuba. the fermentative pattern d was the most frequently found. patterns d and g together were 72 % of the total of strains studied. the combination of the carbohidrate fermentation with serotypping and biotyping allowed a greater differentiation of strains (14 groups). patterns a, b, c and f appeared in children over 6 months of age, and pattern g in the group from 6 to 18. patterns d and g predominated in the bacterial meningoencephalitis. a higher heterogeneity was observed among the strains isolated from acute respiratory infections. some of the advantages of the heaemophilus influenzae strains subtyping method are stressed, such as: simplicity, easiness to be applied and interpreted, and the fact that it is not necessary a qualified personnel or a specialized laboratory for its implementation.
Primer reporte de Neisseria polysaccharea en Cuba
Daymi Guzmán Hernández,Rafael Llanes Caballero,Isabel Martínez Motas,Eduardo A. Valdés Ramos
Revista Cubana de Medicina Tropical , 1999,
Abstract: En el Laboratorio Nacional de Referencia de Neisserias Patógenas del Instituto de Medicina Tropical "Pedro Kourí" se estudiaron 58 cepas identificadas previamente como Neisseria meningitidis, aisladas de portadores nasofaríngeos, en el período 1985-1988. A éstas se les aplicó el esquema de identificación de Knapp, a adiéndole la prueba de la producción de gamma -glutamiltransferasa, esta última y la producción de polisacárido a partir de 5 % de sacarosa permitieron realizar el diagnóstico diferencial entre N. meningitidis y Neisseria polysaccharea. Se logró identificar una cepa de N. polysaccharea, lo que constituye el primer reporte de esta especie en Cuba. The National Laboratory of Reference of Pathogenic Neisserias of Pedro Kourí Tropical Medicine Institute studied 58 strains which had been previously identified as Neisseria meningiditis and isolated from nasopharyngeal carriers from 1985-1998. They were applied the knapp detection scheme plus the glutamiltransferase production test. The latter and the polyscharide production based on 5 % sacarose allowed to make a differential diagnosis between N. meningitidis and Neisseria polyccharea. It was possible to identify a N. polysaccharia strain, a species that is reported for the first time in Cuba.
Caracterización de cepas de Haemophilus influenzae mediante fermentación de azúcares
RAFAEL LLANES CABALLERO,LUIS E. AZAHARES ROMERO,MIRIAM F. PéREZ MONRAS,ALICIA MARTíNEZ DíAZ
Revista Cubana de Medicina Tropical , 1997,
Abstract: Se caracterizaron 64 cepas de Haemophilus influenzae circulantes en Ciudad de La Habana durante 1 a, por el método de fermentación de azúcares, por primera vez en Cuba. El patrón fermentativo D fue el más frecuentemente encontrado. Los patrones D y G en conjunto constituyeron el 72 % del total de cepas estudiadas. La combinación de la fermentación de azúcares con el serotipaje y el biotipaje permitió una mayor diferenciación de las cepas (14 grupos). Los patrones A, B, C y F aparecieron en ni os mayores de 6 meses y el G en el grupo de 6 a 18 meses. En la meningoencefalitis bacteriana predominaron los patrones D y G, existió una mayor heterogeneidad en las cepas aisladas de infecciones respiratorias agudas. Se se alan algunas ventajas de este método de subtipificación de cepas de Haemophilus influenzae como son: la sencillez, facilidad de realizar e interpretar y no requerir de un personal calificado ni de un laboratorio especializado para su ejecución. 64 Haemophilus influenzae strains circulating in Havana City during a year were characterized by the Carbohidrate fermentation method for the first time in Cuba. The fermentative pattern D was the most frequently found. Patterns D and G together were 72 % of the total of strains studied. The combination of the Carbohidrate fermentation with serotypping and biotyping allowed a greater differentiation of strains (14 groups). Patterns A, B, C and F appeared in children over 6 months of age, and pattern G in the group from 6 to 18. Patterns D and G predominated in the bacterial meningoencephalitis. A higher heterogeneity was observed among the strains isolated from acute respiratory infections. Some of the advantages of the Heaemophilus influenzae strains subtyping method are stressed, such as: simplicity, easiness to be applied and interpreted, and the fact that it is not necessary a qualified personnel or a specialized laboratory for its implementation.
Serotipos de Streptococcus pneumoniae en Cuba y progresión de la resistencia a la penicilina
Tora?o Peraza,Gilda T.; Llanes Caballero,Rafael; Pías Solis,Lisania M.; Abreu Capote,Miriam; Valcárcel Sánchez,Marlén;
Revista Cubana de Medicina Tropical , 2010,
Abstract: introduction: since 2006 in cuba, there has been difficult to determine the actual incidente of bacterial meningitis and other invasive diseases caused by streptococcus pneumoniae, as a result of the low percentage of isolation and identification of this agent in the domestic microbiology lab network. objective: to warn of the need of revitalizing the active surveillance of invasive, epidemiological and microbiological pneumoccocic diseases, mainly meningitis and pneumoniae in the phase prior to vaccination. methods: a comparison was made on the circulation of capsulary serogroups/serotypes and the invasive infection-causing s. pneumoniae isolates susceptibility to penicilline in two periods: 1994-2006 and 2007-2009. results: the most frequent serogroups were 3, 6, 7, 9, 14, 17, 18, 19, 20 and 23; however, in the second period, it was observed that others not contained in the available conjugate vaccines (serogrup 15) and associated to antimicrobial resistance (serotype 5) were circulating. on the other hand, increased resistance to penicilline was evident. conclusions: it is required to study a more representative number of isolates in order to assure high effectiveness with the incorporation of some s. pneumoniae conjugate vaccines in the cuban infant vaccination schedule. this will also allow duly assessing increased resistance to penicilline and adopting therapeutical guidelines suited to the cuban setting.
Susceptibilidad antimicrobiana de cepas de Neisseria gonorrhoeae por difusión con discos
Llanes Caballero,Rafael; Acosta Giraldo,Juan C.; Sosa Puente,Jorge; Guzmán Hernández,Daymi; Gutiérrez González,Oderay; Llop Hernández,Alina;
Revista Cubana de Medicina Tropical , 1999,
Abstract: the gonoccocus laboratory of "pedro kourí" tropical medicine institute carried out a study of in vitro susceptibility of neisseria gonorrhoeae to penicillin, tetracycline, cefuroxime ceftriaxone, cefotaxine and ciprofoxacin by means of a disk diffusion method with the culture medium agar base gc plus supplement. in the first phase, the method was standardized and the reference n. gonorrhoeae atcc 49226 strain was used whereas in the second phase, 50 gonococcal strains isolated in 8 provinces during 1995 and 1996 were examined. the results of such standardization confirmed that the antimicrobial susceptibility values were within the allowable limits. 52 and 34 % of strains were resistant to penicillin and tetracycline respectively and all of them showed susceptibility to the rest of evaluated antimicrobial drugs. we recommend the use of the disk diffusion method for surveillance of gonococci resistance to these drugs in our country.
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