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Search Results: 1 - 10 of 160189 matches for " Rafael F. Fonseca "
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Phytotoxin produced by Bipolaris euphorbiae in-vitro is effective against the weed Euphorbia heterophylla
Barbosa Aneli M.,Souza Cristina G. M.,Dekker Robert F. H.,Fonseca Rafael C.
Brazilian Archives of Biology and Technology , 2002,
Abstract: Four virulent strain isolates of the fungus, Bipolaris euphorbiae (previously identified as a Helminthosporium sp.), isolated from host plants in four states within Brazil were screened for the production of phytotoxins that promoted wilting and defoliation of the Brazilian weed, Euphorbia heterophylla, commonly found growing among soyabean crops. Only one isolate, B. euphorbiae Strain I (EUPH petropar from Mato Grosso state), produced phytotoxin in-vitro when grown in stationary culture for 7 d at 28 ° C on minimum salts medium supplemented with 1.5 % glucose as the sole carbon source. Phytotoxin was also produced when the fungal strain was grown on fructose, galactose, mannose, xylose and sucrose. The addition of nitrogen source (yeast extract, peptone or malt extract) to the culture medium did not influence phytotoxin production. The phytotoxin produced by Strain I was most active at pH 6.0, stable between pH 3-9, and was highly thermostable, remaining fully active when heated at 90 ° C for 1 h.
Evaluation of immunogenicity elicited from two DNA vaccine candidates that expresses the prM and E genes of the dengue-3 virus  [PDF]
Sérgio O. de Paula, Rafael F. O. Fran?a, Danielle M. Lima, Nina R. Dutra, Marília B. de Paula, Michelle D. de Oliveira, Leandro L. de Oliveira, Benedito A. L. da Fonseca
Health (Health) , 2010, DOI: 10.4236/health.2010.211193
Abstract: In this work, we report the evaluation of two DNA vaccines against dengue-3 virus (DENV-3). The first construction, called pVAC3DEN3, was engineered inserting the pre-membrane (prM) and envelope (E) gene of DENV-3 truncated with a restriction site between them, as previously described. The second construction was developed cloning the full gene sequence of prM and E from DENV-3 virus in pCI plasmid for mammalian expression and was denominated pVAC1WDEN3. The results showed that both constructions were capable of expressing the prM and E proteins, as demonstrated by ELISA and immunoblotting detection in cell culture transfected with the plasmids. After positive “in vitro” results, the vaccine candidates were used to immunize BALB/c mice and the elicited response was investigated. After immunization by intramuscular inoculation with three doses of each vaccinal clone the animals were sacrificed, the cytokine levels and T cell response were analyzed in the spleens, after three days of culture with stimulus, our analysis showed that the two constructions elicited T cell responses mea- sured by BrdU incorporation assay and high levels of IFN-γ, detected in the supernatant of the cultures. Moreover, both constructions induced detectable titers of neutralizing antibodies in mice. And finally the survival rate of the immunized animals after intracerebral challenge was analyzed, showing a better result in the pVAC3DEN3 group with an 80% survival compared with a 50% survival of the pVAC1 WDEN3. Thus, these data showed that our two constructions were able to induce specific immune response and protects mice against a lethal challenge with DENV-3, and these vaccine candidates can be employed to develop a viable dengue vaccine.
Comparative pollen preferences by africanized honeybees Apis mellifera L. of two colonies in Pará de Minas, Minas Gerais, Brazil
Luz, Cynthia F.P. da;Bacha Junior, Gabriel L.;Fonseca, Rafael L.S. e;Sousa, Priscila R. de;
Anais da Academia Brasileira de Ciências , 2010, DOI: 10.1590/S0001-37652010000200005
Abstract: the aim of this study was to investigate the polliniferous floral sources used by apis mellifera (l.) (africanized) in an apiary situated in pará de minas, minas gerais state, and evaluate the pollen prefences among the beehives. two beehives of langstroth type with frontal pollen trap collectors were used. the harvest was made from september 2007 to march 2008, with three samples of pollen pellets colected per month per beehive. the subsamples of 2 grams each were prepared according to the european standard melissopalynological method. a total of 56 pollen types were observed, identifying 43 genus and 32 families. the families that showed the major richness of pollen types were: mimosaceae (8), asteraceae (6), fabaceae (3), arecaceae (3), euphorbiaceae (3), rubiaceae (3), caesalpiniaceae (2), moraceae (2) and myrtaceae (2). the most frequent pollen types (> 45%) were mimosa scabrella, myrcia and sorocea. the results demonstrated a similarity regarding the preferences of floral sources during the major part of the time. there was a distinct utilization of floral sources among the pollen types of minor frequency. in spite of the strong antropic influence, the region showed a great polliniferous variety, which was an indicative of the potential for monofloral as well as heterofloral pollen production.
Phytotoxin produced by Bipolaris euphorbiae in-vitro is effective against the weed Euphorbia heterophylla
Barbosa, Aneli M.;Souza, Cristina G. M.;Dekker, Robert F. H.;Fonseca, Rafael C.;Ferreira, Dalva T.;
Brazilian Archives of Biology and Technology , 2002, DOI: 10.1590/S1516-89132002000200016
Abstract: four virulent strain isolates of the fungus, bipolaris euphorbiae (previously identified as a helminthosporium sp.), isolated from host plants in four states within brazil were screened for the production of phytotoxins that promoted wilting and defoliation of the brazilian weed, euphorbia heterophylla, commonly found growing among soyabean crops. only one isolate, b. euphorbiae strain i (euph petropar from mato grosso state), produced phytotoxin in-vitro when grown in stationary culture for 7 d at 28 ° c on minimum salts medium supplemented with 1.5 % glucose as the sole carbon source. phytotoxin was also produced when the fungal strain was grown on fructose, galactose, mannose, xylose and sucrose. the addition of nitrogen source (yeast extract, peptone or malt extract) to the culture medium did not influence phytotoxin production. the phytotoxin produced by strain i was most active at ph 6.0, stable between ph 3-9, and was highly thermostable, remaining fully active when heated at 90 ° c for 1 h.
Indica??es de colonoscopia versus achado de pólipos e neoplasias colorretais
Batista, Rodrigo Rocha;Lima, Rafael Ferreira Correia;Fonseca, Marcus Fábio Magalh?es;Todinov, Lilian Ramos;Formiga, Galdino José Sitonio;
Revista Brasileira de Coloproctologia , 2011, DOI: 10.1590/S0101-98802011000100009
Abstract: objective: to analyze colonoscopy indications and the finding of polyps and colorectal neoplasms. methods: a retrospective study of colonoscopies reports that were performed between january, 2009 and march, 2010. the analyzed variables included: age, gender, indication for examination, and found of polyp or tumor. the statistical analysis was accomplished with the chi-square test, with significance at p<0.05. results: four hundred and ninety-three colonoscopy reports of patients between 18 and 94 years, mean age 56.7 years old, and 54% female, were reviewed. the examinations had one or more changes in 47.3%, 17.4% with tumor and 14.8% with polyps. the incomplete colonoscopies corresponded to 24.3%, due to tumor obstruction, fixed kink, poor preparation, stenosis, and agitation. the statistically significant indications for increased finding of colorectal tumor were gastrointestinal bleeding, anemia, chronic wasting syndrome, palpable abdominal tumor, polyposis, and elevation of cea in the postoperative. there were no significant differences between polyps and males or advancing age, neither between the finding of polyps and risk of associated tumor. conclusion: patients with gastrointestinal bleeding, anemia, chronic wasting syndrome, palpable abdominal tumor, polyposis, and increased postoperative cea of colorectal cancer should be prioritized for the colonoscopy study.
Quality of water resources in the Amazon region- Rio Tapajós: assessing the case for chemical elements and physical-chemical parameters
Rafaella Galv?o Miranda,Simone de Fátima Pinheiro Pereira,Danila Teresa Valeriano Alves,Geiso Rafael Fonseca Oliveira
Ambiente e água : An Interdisciplinary Journal of Applied Science , 2009,
Abstract: The Tapajós river is the main rivers of the Pará State and part of the Amazon basin is the largest world river basin however, the population growth and development of agriculture in the region has generated the input of contaminants in this important river. The objective of this study was to evaluate the water quality of the Tapajós river in the presence of metals and physico-chemical parameters based on the CONAMA 357-05 resolution. Seven sampling stations were selected along the river in the surface layer, intermediate and bottom in low rainfall periods. The elements Ca, Mg, Al, Fe, Ba, Mn, Sr, Ti, Ag, Be, Cd, Co, Cr, Cu, Mo, Ni, Pb and Zn were determined using the inductively coupled plasma atomic emission spectrometry (ICP-AES), As was determined by ICP-AES with hydride generation, Hg was determined by cold vapor atomic absorption and the physico-chemical parameters were evaluated using various techniques. In almost all respects the elements Al and Fe are not presented in accordance with the values allowed by Brazilian legislation, with average values of 280.01 ± 294.69 g/L and 564.89 ± 326.5 g/L, respectively. There were excellent correlations between Ca and Mg, Ca and Sr, Mg and Sr, Mn and Ba, Sr and OD, Mg and OD. The Tapajós River showed high levels of Al and Fe that compromises the quality of this important river of the Amazon and can cause problems for the health of the population.
Determina??o espectrofotométrica do arsênio em solo da cidade de Santana-AP usando o método do dietilditiocarbamato de prata (SDDC) modificado
Pereira, Simone de Fátima Pinheiro;Oliveira, Geiso Rafael Fonseca;Oliveira, Johny da Silva;Silva, Juliane da Silva e;Sousa Junior, Pedro Moreira de;
Acta Amazonica , 2009, DOI: 10.1590/S0044-59672009000400023
Abstract: several tons of manganese residues containing arsenic, generated by a mining company, were used as streets landfill in santana-ap city. the possibility of exposure of people living in these villages has led to the study of quantification of total arsenic in the soil. after digestion, the levels of arsenic were quantified by molecular absorption spectrophotometry using a hydrides generation automatic system (hg-mas), dicyclohexilamine/chcl3 as silver diethyldithiocarbamate (sddc) solvent and kbh4 as reducer. the method presented good results with sensitivity (ε) of 1.10 104 l.mol-1.cm-1, stability of 2.96 % and other advantages in relation to the official method. the method was applied to the soil standard samples with recoveries of the 98.82 % (n=10). the soil analysis showed that 94.74 % samples showed arsenic concentrations above the value published by cetesb for residential land (50 mg.kg-1) with the average value of 682.96 mg.kg-1, ranging of 48.08 mg.kg-1 at 1,713.00 mg.kg-1 shows that the soil arsenic contamination.
Correla??o entre a resistência a pirazinamida e a resistência a outros fármacos antituberculose em cepas de Mycobacterium tuberculosis isoladas em um hospital de referência
Fonseca, Leila de Souza;Marsico, Anna Grazia;Vieira, Gisele Betzler de Oliveira;Duarte, Rafael da Silva;Saad, Maria Helena Féres;Mello, Fernanda de Carvalho Queiroz;
Jornal Brasileiro de Pneumologia , 2012, DOI: 10.1590/S1806-37132012000500013
Abstract: the correlation between resistance to pyrazinamide (pza) and resistance to other first-line antituberculosis drugs was investigated in 395 mycobacterium tuberculosis strains isolated from clinical specimens, representing 14% of the overall number of m. tuberculosis isolates obtained between 2003 and 2008 at the laboratory of a referral university hospital for tuberculosis. a high correlation was found between resistance to pza and multidrug resistance, as well as between pza resistance and resistance to rifampin, isoniazid, and ethambutol (p < 0.01 for all). these results highlight the importance of performing pza susceptibility testing prior to the prescription of this drug in order to treat drug-resistant and multidrug-resistant tuberculosis.
Validation of HPLC, DPPH? and nitrosation methods for mesalamine determination in pharmaceutical dosage forms
Rafael, Janice Aparecida;Jabor, José Roberto;Casagrande, Rúbia;Georgetti, Sandra Regina;Borin, Maria de Fátima;Fonseca, Maria José Vieira;
Revista Brasileira de Ciências Farmacêuticas , 2007, DOI: 10.1590/S1516-93322007000100012
Abstract: mesalamine (5-aminosalicylic acid, 5-asa) is used because of its local effects in the treatment of inflammatory bowel disease. therefore, the aims of this work were to compare and validate three analytical methods for the quality control of commercial coated tablets containing 5-asa: high performance liquid chromatography (hplc), 1,1-diphenyl-2-picrylhydrazyl radicals (dpph?) and nitrosation. the parameters linearity, precision and accuracy were studied in this work. hplc with ultraviolet detection at 254 nm was carried out with a c18 column and a mobile phase constituted of 30 mmol/l monobasic phosphate buffer (ph 7.0) and methanol (70:30; v/v), with 25% tetrabutylammonium hydrogen sulphate. the dpph? method was performed at 517 nm and using 100 mmol/l acetate buffer, ph 5.5, ethanol and 250 μmol/l ethanolic solution of dpph?. the nitrosation method was accomplished by using a platinum electrode and standard 0.1 mol/l sodium nitrite as titrant solution. repeatability (intra-day) and intermediate precision (inter-day), expressed as rsd, were lower than 3%. the experimental recoveries were between 72.5 and 99.9%. statistical analysis by one-way anova, followed by the multiple comparison test of bonferroni showed no significant difference among the three methods. all proposed methods can be used for the reliable quantitation of 5-asa in pharmaceutical dosage forms.
Production of Biomass-Degrading Multienzyme Complexes under Solid-State Fermentation of Soybean Meal Using a Bioreactor
Gabriela L. Vitcosque,Rafael F. Fonseca,Ursula Fabiola Rodríguez-Zúñiga,Victor Bertucci Neto
Enzyme Research , 2012, DOI: 10.1155/2012/248983
Abstract:
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