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Search Results: 1 - 7 of 7 matches for " RONDANG ROEMIATI SOEGIANTO "
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Isolation and Characterization of Chelonia mydas Myoglobin
MOHAMAD SADIKIN,RINI PUSPITANINGRUM,SEPTELIA INAWATI WANANDI,RONDANG ROEMIATI SOEGIANTO
HAYATI Journal of Biosciences , 2012,
Abstract: Green sea turtle – Chelonia mydas is a lung respiration animal that is able to dive and stay uder sea water for hours without needing to surface for oxygen. As oxygen supply in muscles is assured by myoglobin, we propose to study some characteristic of this muscle protein in green sea turtles. To achieve this objective, pure green sea turtle myoglobin has to be made available. Therefore, our first task is to purify this muscle protein from green sea turtles. Skeletal muscles from 3 green sea turtle hatchlings were studied icroscopically and biochemically. Microscopy observation showed a general structure of striated muscle. Biochemical studies revealed that green sea turtle myoglobin could be more purely isolated to a certain degree by ephadex G-75 gel filtration and purified by immunoaffinity gel chromatography rather than direct purification by DEAE-Sepharose ion exchange chromatography technique. SDS-PAGE analysis showed that green sea turtle myoglobin ran together with horse myoglobin as 17 kDa molecular weight proteins.
Blood Gasses Contents of Green Turtle (Chelonia mydas) Hatch Treated by Different Temperatures
RINI PUSPITANINGRUM,SEPTELIA INAWATI WANANDI,RONDANG ROEMIATI SOEGIANTO,MOHAMAD SADIKIN
HAYATI Journal of Biosciences , 2011,
Abstract: The aim of this research was to gain the profile of blood gasses of green turtle (Chelonia mydas) hatch. Blood gas of the green turtle was analysed after exposuring them at 28 oC and 50% of humidity for 24 hours in a pvc tube and at 34 oC under sunlight exposured with 47% of humidity for 30 minutes. The result showed the different values of blood gas contents. This result showed indication of metabolism activities and poikilothermic adaptation of green turtle hatch. This information can be used to support for turtle hatchery in Indonesia.
Myoglobin Expression in Chelonia mydas Brain, Heart and Liver Tissues
RINI PUSPITANINGRUM,SEPTELIA INAWATI WANANDI,RONDANG ROEMIATI SOEGIANTO,MOHAMAD SADIKIN
HAYATI Journal of Biosciences , 2010,
Abstract: An understanding of the underpinning physiology and biochemistry of animals is essential to properly understand the impact of anthropogenic changes and natural catastrophes upon the conservation of endangered species. An observation on the tissue location of the key respiratory protein, myoglobin, now opens up new opportunities for understanding how hypoxia tolerance impacts on diving lifestyle in turtles. The respiratory protein, myoglobin has functions other than oxygen binding which are involved in hypoxia tolerance, including metabolism of reactive oxygen species and of the vascular function by metabolism of nitric oxide. Our work aims to determine whether myoglobin expression in the green turtle exists in multiple non muscle tissues and to confirm the hypothesis that reptiles also have a distributed myoglobin expression which is linked to the hypoxiatolerant trait. This initial work in turtle hatch Chelonia mydas confirms the presence of myoglobin transcriptin brain, heart and liver tissues. Furthermore, it will serve as a tool for completing the sequence and generating an in situ hybridization probe for verifying of cell location in expressing tissues.
Analysis of the chaos dynamics in(Xn,Xn+1)plane
S. Soegianto,The Houw Liong
Physics , 2002,
Abstract: in the last decade, studies of chaotic system are more often used for classical choatic system than for quantum chaotic system, there are many ways of observing the chaotic system such us analyzing the frequency with Fourier transform or analyzing initial condition distance with Liapunov Exponent, this paper explains dynamic chaotic process by observing trajectory of dynamic system in (Xn,Xn+1)
Blooming Stimulation of Microcystis in Sutami Reservoir Using Nutrients Nitrate and Phosphate in Different ratio
Catur Retnaningdyah,Suharjono,Agoes Soegianto,Bambang Irawan
Journal of Tropical Life Science , 2010,
Abstract: Microalgae Cyanobacteria from the genus Microcystis has the potential to bloom in Sutami reservoirs that located in Malang, Indonesia at a particular time. Microcystis is a notorious species because it can produce toxins that are dangerous to other organisms. The objective of this research is to determine the influence of different ratios of Nitrate and Phosphate on the growth of Microcystis in Sutami reservoir. The study was conducted from April to November 2009 and was carried out in situ in the reservoir Sutami with quasi experiment using a completely randomized design. Sutami reservoir water which already contained plankton community therein, including Microcystis were treated with five variations ratio of Nitrate and Phosphate (10, 20, 40, 80, and 160) and untreated water as control. Experiments carried out by using a transparent plastic bag diameter 1 m and 1.5 m long, inserted into the water and be immersed in the water by using bamboo. Calculation of Microcystis abundance and physic-chemical quality of water is done every three days for a month. The results showed that the higher concentration of orthophosphate in the media water of Sutami reservoir have the maximum abundance of Microcystis. Abundance of Microcystis grown in Sutami reservoirs have positively correlated with actual levels of nitrate, nitrite, total phosphate, conductivity, pH, temperature, ratio of Nitrate to Phosphate treatment and water KMnO4 value.
PENENTUAN KOMPOSISI BAHAN BAKU OPTIMAL PRODUK KECAP X DENGAN METODE TAGUCHI
Julianingsih Julianingsih,Debora Anne Yang Aysia,Donny Soegianto
Jurnal Teknik Industri , 2004,
Abstract: The research aimed at getting ingredient composition of soy sauces that preferred by consumers, by means of Taguchi method. The first organoleptik test was carried out to find out consumers' responses to tastes, colors, and thickness of X soy sauces relative to other soy sauces. The results were applied as a reference in pre-experiment. Experiment results indicated that soy sauce whose composition was 23332233 was the most preferable soy sauce by consumers. The proposed' soy sauce was subject to final organoleptik test to find out consumer preference and position of the proposed' soy sauces. The results indicated that 65.4% of respondents liked the proposed' soy sauces. Tastes and thickness of the proposed' soy sauces have been more preferable by consumers relative to other soy sauces. Abstract in Bahasa Indonesia : Penelitian ini bertujuan untuk mendapatkan komposisi bahan baku kecap yang disukai konsumen, dengan menggunakan metode Taguchi. Uji organoleptik pertama dilakukan untuk mengetahui respon konsumen terhadap rasa, warna dan kekentalan kecap X terhadap kecap pesaing. Hasilnya digunakan sebagai acuan pre eksperimen. Hasil eksperimen menunjukkan bahwa kecap dengan komposisi 23332233, paling disukai konsumen. Kecap usulan diuji organoleptik akhir untuk mengetahui kesukaan konsumen dan posisi kecap usulan terhadap kecap pesaing. Hasilnya, 65,4% responden menyukai kecap usulan. Untuk rasa dan kekentalan, kecap usulan lebih disukai daripada kecap pesaing. Kata kunci: Taguchi, Rasa, Warna, Kekentalan, Organoleptik
DROUGHT RESISTANT SELECTION ON SOYBEAN SOMACLONAL VARIANTS
Wahyu Widoretno,Estri Laras Arumingtyas,Nur Basuki,Andy Soegianto
Agrivita : Journal of Agricultural Science , 2012,
Abstract: This research was conducted to evaluate the yield potential of 19 somaclonal variants resulting from in vitro selection when planted under drought stress condition in the field. Field test was done by planting the variants, the parents, and checked varieties in the field during dry season, and was irrigated once a week for non-stress and once two weeks for drought stress treatment. Split-plot design arranged in a factorial (2 x 28) with three replications was used in this research. Obser-vations were done on yield and yield components. Analysis of variance was used to see the difference between treatments and then it was continued with analysis using Honestly Significant Difference test to find out the best treatments. There was no interaction between genotype and drought stress on seed yield. Different genotypes showed a significant difference on this character. It indicated that the yield potential of selected variants was not affected by drought stress treatment. This research gave 10 variants having the potential to be developed as drought resistant genotypes. However, these ten potential geno-types need to be tested further in field trial to find out the yield adaptability and stability and their resistance to drought stress.
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