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Search Results: 1 - 10 of 49654 matches for " REN Sui-zhou "
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Isolation and Identification of petroleum degrading strains and the diversity of microbes in petroleum-contaminated soils
石油降解菌的分离鉴定及石油污染土壤的细菌多样性

REN Sui-Zhou,
任随周

生态学报 , 2005,
Abstract: Four petroleum-contaminated soil samples at different degree were collected and used for isolation of petroleum-degrading bacterium.28 petroleum-degrading strains were screened and identified as Brevibacterium,Pseudomonas,Micrococcus and Plesiomonas,respectively.There are more petroleum-degrading bacteria in heavy petroleum-contaminated soil than that in light contaminated soil,and Brevibacterium and Pseudomonas are dominant petroleum-degrading bacteria.A culture-independent molecular biological method was ...
The microbial population in an anaerobic baffled reactor for the treatment of dye wastewater
处理印染废水的厌氧折流板反应器中的微生物种群组成及分布规律

REN Sui-Zhou,
任随周

生态学报 , 2005,
Abstract: An anaerobic baffled reactor (ABR) provides spatial separation between acidogenic and methanogenic populations, and thus allows different bacterial groups to develop under their optimal conditions. It essentially behaves as a two-phase system. Most of the past studies on microbial populations had focused on methanogenesis. The most common observation is a shift between the two acetoclastic methanogens Methanosarcina sp. and Methanosaeta sp. However, little has been done on the population of acidogenesis. Besides, there are very few examples of using ABR to treat printing and dyeing wastewater although it is known that anaerobic process is a promising alternative to decolorize the dye waste. In this study a 4L ABR containing 12 compartments was used to treat high strength, dark printing and dyeing wastewater. It has internal dimensions of 1200 mm long, 100 mm wide and 400 mm deep. The objective of this study was to identify and quantity the dominant populations in the sludge of each compartment, as well as to analyze the distribution of the physiologically important population members: decolorizing bacteria and aniline-degrading strains. The genus of Bacillus,Acinetobacter,Comamonas,Pseudomonas and Aquaspirillum are the dominant population. Bacillus is the most dominant, and followed by Acinetobacter and Comamonas. In fact, the population of each genus is different various compartments. At the front end, the microbial quantity is higher, but the main population decreases in number toward the end of ABR. The populations of anaerobes in each compartment were analyzed by both plate count under anaerobic station and measurement of methane production rate. The anaerobic populations increase firstly, and then decrease. More than 547 strains, including 174 dyes degrading and 231 aniline-degrading strains, were screened and analyzed for their decolorization and aniline-degrading abilities. The results indicate that a higher decolorizing population existed in the front than in the rear compartments. On the other hand, the aniline-degrading strains were less in the front and more in the rear compartments. The aniline content and its color density in different compartments were also analyzed. The results show that the aniline concentration increased with the degradation of azo dyes by the decolorizing strains in the treatment process. This allowed more aniline-degrading strains to develop, resulting in more aniline degradation under the anaerobic condition prevailing in the second half of ABR. The concentration of aniline was the highest when wastewater was being decolorized,then it gradually decreased. These results provide a useful basis for the ABR process optimization in high strength, dark color printing and dyeing wastewater.
Over-expression of Highly Active Triphenylmethane Dyes Decolorization Enzyme (TpmD) Induced by Lactose Instead of IPTG in Escherichia coli BL21 (DE3)
乳糖替代IPTG诱导脱色酶TpmD基因在大肠杆菌中的高效表达

CHEN Liang,REN Sui-Zhou,XU Mei-Ying,SUN Guo-Ping,
陈 亮
,任随周,许玫英,孙国萍

微生物学通报 , 2009,
Abstract: 本文考察了乳糖代替IPTG诱导三苯基甲烷类染料脱色酶TpmD在大肠杆菌BL21(DE3)中表达的可行性, 分别对用乳糖作为诱导剂时的诱导时机、乳糖浓度、诱导持续时间和添加方式进行优化并与IPTG诱导的差异等方面进行了比较分析, 确定了乳糖诱导的最佳条件。结果表明, 在工程菌对数生长中期(OD600约为0.8)添加终浓度为0.4 mmol/L的乳糖诱导6 h的条件下能获得最大量的目的蛋白和菌体量。由于乳糖可以作为碳源被菌体利用, 分批添加乳糖效果优于一次性添加。乳糖诱导条件下目的蛋白表达量占总蛋白的35.62%, 与IPTG诱导条件下的35.03%无明显差异。乳糖诱导后外源蛋白的表达时间有所滞后, 但收获的菌体量高于IPTG诱导, 显示出了乳糖同样是一种T7启动子的廉价高效诱导剂, 可以代替昂贵的IPTG用于脱色酶TpmD的规模化发酵, 同时也为其他重组蛋白的生产提供了有益的参考和借鉴。
Properties of a triphenylmethane dyes decolorization enzyme (TpmD) from Aeromonas hydrophila strain DN322
细菌脱色酶TpmD的酶学特性研究

REN Sui-zhou,GUO Jun,CEN Ying-hua,SUN Guo-ping,
任随周
,郭俊,王亚丽,岑英华,孙国萍

微生物学报 , 2006,
Abstract: A novel bacterial enzyme for decolorization of triphenylmethane dyes from Aeromonas hydrophila strain DN322 was purified and named TpmD.The basic properties of this enzyme including molecular weight,isoelectric point Km as well as the optimum temperature and pH were determined and the enzyme was identified as an NADH/NADPH-dependent oxygenase in previous research.Based on previous results,the effect of different inhibitor including Vc,metyrapone,rotenone,antimycin A and NaN-3 as well as the effect of FAD and FMN on the activity of TpmD were measured.The results indicated that the activity of the decolorization enzyme was inhibited by Vc and metyrapone in a concentration-dependent manner,but wasn't inhibited by rotenone,antimycin A and NaN-3.The activity of the decolorization enzyme was not enhanced by addition of FAD or FMN.The solution of the enzyme protein displayed only a single peak at 408nm in the Soret region,a characteristic peak of porphyrin,but did not show the characteristic peak of the cytochrome P450 proteins at 450nm in sodium dithionite(DTN)-reduced enzyme solution after treatment with carbon monoxide.The amino acid sequence of N-terminal of TpmD provided further evidence that the enzyme is an oxygenase.All these results suggest that decolorization enzyme TpmD is a new hemo-containing oxygenase.The decolorization enzyme would be a good material for further research of the enzymological mechanism of triphenylmethane dyes decolorization by bacteria.
Recent Advances in Microbial Decolorization of Triphenylmethane Dyes
微生物对三苯基甲烷类染料脱色的研究进展

ZHANG Pei-Pei,REN Sui-Zhou,XU Mei-Ying,SUN Guo-Ping,
张培培
,任随周,许玫英,孙国萍

微生物学通报 , 2009,
Abstract: Triphenylmethane dyes are widely used in textile dyeing, medicine, biological staining, paper, leather, food and cosmetic industries. Their special stable chemical structures make them difficult to be degradated and decolorized. The biodecolorization research of triphenylmethane dyes will provide the fundamental knowledge for the treatment of dye-containing wastewater and bioremediation of dye-contaminated environment. In this paper, the recent research progress in biodegradation and decolorization of triphenylmethane dyes by microorganisms, such as bacteria, actinomycetes, fungi and alga, as well as the functioning enzymes and their coding genes, were reviewed. The decolorization mechanisms and degradation pathways of triphenylmethane dyes were discussed based on the decolorizing intermediate products of different microbes. The development and application of triphenylmethane dyes biodecolorization and biodegradation were also prospected.
Properties of a triphenylmethane dyes decolorization enzyme TpmD from Aeromonas hydrophila strain DN322
细菌脱色酶TpmD对三苯基甲烷类染料脱色的酶学特性研究

REN Sui-zhou,GUO Jun,WANG Ya-li,CEN Ying-hua,SUN Guo-ping,
任随周
,郭俊,王亚丽,岑英华,孙国萍

微生物学报 , 2006,
Abstract: A novel bacterial decolorization enzyme for triphenylmethane dyes from Aeromonas hydrophila strain DN322 was purified, named TpmD. The purified enzyme catalyzes the decolorization of several triphenylmethane dyes, i.e., crystal violet, basic fuchsin, brilliant green and malachite green. The enzyme was identified by the clear transparent band development of zymogram stained with crystal violet, basic fuchsin, brilliant green and malachite green after polyacrylamide gel electrophoresis (PAGE) respectively. The decolorization enzyme was enzymologically characterized. The results showed that the molecular weight of TpmD is 29.4kDa and its isoelectric point (pI) is 5.6. The maximal activity of TpmD for above four triphenylmethane dyes was observed at 50 degrees C - 55 degrees C and pH 7.4 - 8.0. The temperature for losing half of the activity (t1/2) within 4h is 62 degrees C. The activities of decolorization enzyme are relatively stable at pH range of 5.5 - 9.0. The K(m) and V(max) of TpmD for decolorizing crystal violet, basic fuchsin, brilliant green and malachite green are 24.3, 40.6, 54.2, 68.5 micromol/L respectively, V(max) are 19.6, 74.1, 82.8, 115.6 micromol x L(-1) x s(-1) respectively. Both NADH/NADPH and molecular oxygen are necessary for the enzyme to decolorize triphenylmethane dyes, indicate the enzyme is an NADH/NADPH-dependent oxygenase.
Isolation and Characterization of a Facultative Anaerobic Aniline-Degrading Bacterium
兼性厌氧苯胺降解菌的分离鉴定及其特性

ZENG Guo-qu,REN Sui-zhou,CAO Wei,HU Jin-cai,LIN Lu-jing,SUN Guo-ping,
曾国驱
,任随周,曹渭,胡锦财,林璐菁,孙国萍

环境科学 , 2006,
Abstract: An aniline-degrading bacterium (designated strain AN29) was isolated from dyeing wastewater process (anaerobic baffled reactor, ABR) with the capability of utilizing aniline as sole carbon source and nitrogen source. It was identified as Pseudomonas sp. based upon the phenotypic properties and a partial analysis of the 16S rDNA. The strain could degrade aniline under the aerobic and anaerobic conditions, the optimal initial pH 6.5 - 8.0, a temperature of 37 degrees C, and initial aniline concentrations of 500 - 2 000 mg/L with maximum concentration of 4 000 mg/L respectively.
Isolation and Characterization of two Aniline-degrading Strains and Compare of Functional Genes
2株苯胺降解的分离鉴定及其降解特性研究

Ren Sui-zhou,Guo Jun,Zeng Guo-qu,Cen Ying-hua,Sun Guo-ping,
任随周
,郭俊,曾国驱,岑英华,孙国萍

环境科学 , 2006,
Abstract: Two aniline-degrading bacterial strains,AN30 and DN425,were isolated from activated sludge of textile-printing wastewater treatment plant and identified as Pseudomonas sp.and Shewanella sp.,respectively.Under shaking condition,250(mg/L) aniline was removed 96.1% and 13.8% within 72h by strains AN30 and DN425,respectively.Under static condition,their degrading rates were 39.6% and 8.6%,respectively.Under static condition,the decolorizing rate of strain DN425 reached 96% for azo dye Great Red GR in 4h,exhibiting a remarkable color removal capability.However,strain AN30 was not capable of decolorizing Great Red GR.With two sets of specific primers for tdnQ gene and fre gene, the two genes were detected by PCR amplification.The results indicated the two strains possess both tdnQ gene and fre gene.
Study on Decolorization of Triphenylmethane Dyes by DTT
DTT对三苯基甲烷染料脱色的研究

PAN Tao,LIU Da-wei,REN Sui-zhou,GUO Jun,SUN Guo-ping,
潘涛
,刘大伟,任随周,郭俊,孙国萍

环境科学 , 2012,
Abstract: Decolorization of triphenylmethane dyes by DTT was researched. For malachite green, content of DTT in reaction system was optimized to investigate the quantitative relation between DTT and malachite green and the decolorization capacity of DTT was confirmed. Effect of pH of reaction system on reducibility of DTT was explored. The results indicated 4 mol malachite green could be decolorized by 1 mol DTT averagely within 1 min, when pH of the reaction system was above 5.The decolorization rate was up to 97%. Decolorization product of malachite mreen was corroborated to be its leuco form by HPLC analysis. Some insoluble compounds, which could be the complex products of leuco malachite green with DTT, were formed during the decolorization reaction. Decolorization of crystal violet, brilliant green and basic fuchsin by DTT was tested further, and the decolorization rates were all above 85%, which suggested DTT was a broad-spectrum decolorization agent for triphenylmethane dyes.
胶东玲珑金矿田构造蚀变岩测量与成矿预测

武际春, 吕古贤, 霍庆龙, 杨美汪, 刘维民, 朱随洲
WU Ji-Chun
, LV Gu-Xian, HE Qiang-Long, YANG Mei-Hong, LIU Wei-Min, SHU Sui-Zhou

- , 2015, DOI: 10.13745/j.esf.2015.04.010
Abstract: 对几十年来地质找矿工作进行总结,支撑深部找矿的重大难题,经过多年的实际观测和分析研究,完成了“山东省招远市玲珑金矿构造蚀变成矿图”。玲珑金矿田发育玲珑焦家式金矿——陆内岩浆期后热液蚀变剪切带硫化物石英脉黄铁绢英岩蚀变破碎岩型金矿。该类矿床受断裂破碎带影响,成矿地质体是黄铁绢英岩质蚀变岩带。研究应用构造蚀变岩的地质测量和研究方法,实测了大量的构造岩相剖面,开展了1∶2 000和1∶10 000的填图。结果显示:(1)矿田处于滦家河岩体外接触带,赋矿围岩主要是玲珑似片麻状黑云母花岗岩。花岗岩的似片麻理走向显示北西西至南东东向分布,带有胶东基底的构造痕迹。(2)断裂蚀变矿化带控制了金矿的分布规律,它们受到后期构造的改造。(3)图中从西到东、从北到南,流体交代蚀变岩的规模都逐渐增大,因此,通过断裂蚀变岩的发育情况,可知较大矿床集中在矿田的东南部。东南部构造及蚀变带规模大,矿体埋藏深,有良好的成矿远景。“山东省招远市玲珑金矿构造蚀变成矿图”的编制,反映了区内控矿构造的新资料。关于矿田找矿预测的靶区得到了探矿工程的验证,并有力地支持了今后一个阶段的深部找矿问题。以成矿地质事件为基础的大比例尺构造岩相地质填图及找矿方法,具有重要的成矿研究和深部外围找矿意义,值得进一步深化和推广。
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