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Search Results: 1 - 10 of 233 matches for " RATIH DEWI HASTUTI "
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Endophytic Streptomyces spp. as Biocontrol Agents of Rice Bacterial Leaf Blight Pathogen (Xanthomonas oryzae pv. oryzae)
RATIH DEWI HASTUTI,YULIN LESTARI,ANTONIUS SUWANTO,RASTI SARASWATI
HAYATI Journal of Biosciences , 2012,
Abstract: Xanthomonas oryzae pv. oryzae (Xoo), a causal agent of bacterial leaf blight (BLB), is one of the most important pathogens of rice. The effectiveness of ten Streptomyces spp. isolates in suppressing Xoo disease was assessed in planta and in vitro. In planta experiments were carried out in a greenhouse and arranged in a randomized completely block design (RCBD) with three replications. Twenty treatments were tested which included plants inoculated with both Streptomyces spp. and Xoo, and plants inoculated with only Streptomyces spp. Plants inoculated with Xoo and sprayed with a chemical bactericide, and plants inoculated with only Xoo served as positive controls, whereas plants not inoculated with either Streptomyces spp. or Xoo were used as negative controls. The results showed that the effect of endophytic Streptomyces spp. on BLB disease expressed as area under disease progress curve (AUDPC) was not significantly different to that on control plants (P > 0.05). However, plants inoculated with endophytic Streptomyces spp. were significantly taller and produced higher tiller number than control plants (P < 0.05). Streptomyces spp. isolate AB131-1 gave the highest plant height. In vitro studies on biocontrol mechanisms of selected Streptomyces spp. isolates showed that isolate LBR02 gave the highest inhibition activity on Xoo growth, followed by AB131-1 and AB131-2. Two isolates (AB131-1 and LBR02) were able to produce chitinase, phosphatase, and siderophore which included biocontrol characteristics. Morphological and colonization studies under SEM and light microscopy confirmed that the three isolates were endophytic Streptomyces spp. from different species. These studies found that the paddy plant which was inoculated with endophytic Streptomyces spp. AB131-1 and infected by Xoo could increase the height of plant and number of tillers.
TERITORIALITAS PERMUKIMAN TEPI SUNGAI CODE
Hastuti Saptorini
Dimensi : Journal of Architecture and Built Environment , 2006,
Abstract: Spontaneous settlement of waterfront Code River as unplanned development have demonstrated the spacial behaviour phenomena. The occupants have utilised the expansion lands for domestic, economic, and social activities, both individually and communally. These phenomena have implicated to the numerous senses of territory by signing the area. These spasial responses which contain territorial development meaning have special fenomenal studies which have encouraged architectural meaning. The case study data adopted from author's research group with Arif Budi Solihah, ST in 2004 by the title 'Typology and Morphology Study of Waterfront Housing Caracters; Code River Housing Case Study which funded by High Education General Directorate, National Education Department numbered 199/P4T/DPPM/DM,SKW,SOSAg/ III/2004, have indicated some findings. Authorizing of "independent land" mechanism as research findings show that housing expansion to public space towards the river have utilised in accommodating domestic, social and economic activities. These were signed by some hanging tools and landscape development which are built by two reasons. First, the land status that is encouraging people use riverbank walkway as part of their houses territory. Secondly, the expansion process, both individually and communally, represents the cultural, economic and social demand. Abstract in Bahasa Indonesia : Permukiman spontan di tepi sungai Code merupakan manifestasi perilaku spasial yang diwujudkan melalui ekspansi lahan "kepemilikan" untuk kepentingan kegiatan domestic, ekonomi, dan social, baik yang bersifat individu maupun komunal. Respons perilaku ini telah menggejala melalui "penguasaan" lahan yang bersifat teritoris dalam bentuk pemanfaatan area untuk variasi kegiatan dengan berbagai "penandaan". Sehingga, bentuk pengolahan lahan yang mengandung makna "pembangunan" teritori tersebut merupakan kajian fenomena spasial yang memperkaya referens makna arsitektural. Melalui data yang dianalisis kembali dari penelitian kelompok bersama Arif Budi Sholihah, ST tahun 2004 dengan judul Studi Tipologi Morfologi Karakter Permukiman Tepian Sungai; studi Kasus permukiman Tepi Sungai Code Yogyakarta, yang didanai oleh Direktorat Jenderal Pendidikan Tinggi Depdiknas bernomor 199/P4T/DPPM/ DM,SKW,SOSAg/III/2004, mengindikasikan beberapa temuan. Penguasaan lahan yang ditandai dengan alat/bahan yang dipasang di tempat kegiatan dan pembangunan batas-batas area kegiatan, kolam, dan penanaman vegetasi, disebabkan oleh 2 kondisi. Pertama, karena status lahan yang bersifat "independent" sehingga memu
Correlation between Floor Space and Sanitation of Cage with Mastitis Disease Occurrence on The Dairy C
Sri Hastuti
Journal of Animal Production , 2000,
Abstract: The objective of research was to know the correlation between floor space and sanitation of cage with mastitis disease occurance on the dairy cattle. Sixtiy infected mastitis disease dairy cows were used in the research in the Banyumas regency. Survey was applied in this experiment and Linier Multiple Regression was used. The result showed that there were correlations between floor space and sanitation of cage with mastitis which follow the regression line Yi = 15,355 + 1,087 X1 – 0,249 X2 (Animal Production 2(1): 9-12Key Words: mastitis, floor space, and sanitation
PENELUSURAN REGISTRASI PENDERITA KANKER SERVIKS DI DKI JAKARTA YANG MENDAPAT PENGOBATAN DI RSCM PADA TAHUN 1990
Ratih Oemyati
Media of Health Research and Development , 2012,
Abstract: Saat ini dari setiap 100.000 penduduk Indonesia terdapat kurang lebih 100 penderita baru kanker setiap tahun. Di RSCM, kanker serviks merupakan penyebab kematian terbanyak diantara kematian kanker ginekologis yaitu 66%. Sampai saat ini data/informasi mengenai jumlah kematian maupun survival pasien kanker khususnya kanker serviks belum diketahui dengan baik. Oleh karena itu, perlu dilakukan survei alamat penderita kanker serviks yang telah mendapat pengobatan. Tujuan tinjauan ini secara umum memperoleh data gambaran pasien (yang masih hidup) setelah lima tahun yang lalu mendapat pengobatan dari kanker serviks dan secara khusus untuk mengetahui faktor-faktor hambatan penelusuran alamat pasien kanker serviks. Sampel yang diambil adalah pasien kanker serviks yang masih hidup dan berobat di RSCM pada tahun 1990 dan beralamat di Jakarta, mengingat survival pasien kanker adalah lima tahun. Kemudian dari catatan medik penderita kanker serviks akab ditelusuri alamatnya.
KAJIAN EPIDEMIOLOGIS PENYAKIT PARU OBSTRUKTIF KRONIK (PPOK)
Ratih Oemiati
Media of Health Research and Development , 2013,
Abstract: Abstrak Penyakit Paru Obstruktif Kronis (PPOK) sangat kurang dikenal di masyarakat. Di Amerika Serikat pada tahun 1991 diperkirakan terdapat 14 juta orang menderita PPOK, meningkat 41,5% dibandingkan tahun 1982, sedangkan mortalitas menduduki peringkat IV penyebab terbanyak yaitu 18,6 per 100.000 penduduk pada tahun 1991 dan angka kematian ini meningkat 32,9% dari tahun 1979 sampai 1991. WHO menyebutkan PPOK merupakan penyebab kematian keempat didunia yaitu akan menyebabkan kematian pada 2,75 juta orang atau setara dengan 4,8%. Selain itu WHO juga menyebutkan bahwa sekitar 80 juta orang akan menderita PPOK dan 3 juta meninggal karena PPOK pada tahun 2005. Kajian ini bertujuan untuk mengukur prevalensi PPOK, tingkat keparahan, serta untuk mengidentifikasi tipe PPOK, faktor risiko, morbiditas dan mortalitas, dampak PPOK dan biaya pengobatan. Penelitian ini merupakan review PPOK berdasarkan data kepustakaan dan jurnal dengan fokus penulisan PPOK, yang meliputi; gejala, klasifikasi, prevalensi, faktor risiko, morbiditas dan mortalitas, dampak PPOK, pengobatan dan biaya pengobatan PPOK. Berdasarkan kajian tipe PPOK ada dua yaitu bronchitis kronik dan emphysema. Di Asia Tenggara diperkirakan prevalensi PPOK sebesar 6,3% dengan prevalensi tertinggi ada di negara Vietnam (6,7%)dan RRC (6,5%). Faktor risiko antara lain merokok; polusi indoor, outdoor, dan polusi di tempat kerja; genetik; riwayat infeksi saluran napas berulang. Ada 4 indikator tingkat keparahan berdasarkan ATS (American Thoracic Society). Keterbatasan aktivitas pada pasien PPOK, penurunan berat badan, peningkatan risiko penyakit kardiovaskuler, osteoporosis dan depresi merupakan akibat PPOK.Dibutuhkan sekitar $ 18 miliar biaya langsung dan biaya tidak langsung sekitar $14.1 miliar dalam penanggulangan PPOK di Eropa. Kata Kunci: PPOK, faktor risiko, mortalitas Abstract Chronic Obstructive Pulmonary Disease (COPD) was unknown diseases. It predicted 14 million COPD’s patient in 1991 in USA,in the other hand it raised to 41.5% compare with in 1982.Mortality rate have raised up 32.9% from 1979 to 1991. World Health Organization (WHO) assumed that COPD was in fourth ranks of cause of death in the world, would have 2.75 million mortality or equivalence with 4.8%. Otherwise WHO predicted 80 million people had COPD that 3 million among of them would be death in 2005. The aim of this study to measure COPD prevalenced, degree of severity, COPD types, risk factors, morbidity and mortality, impact of COPD and cost of health care in COPD. The data wasexplored of review COPD based on literature and journal that
INTERESTERIFIKASI ENZIMATIS PALM STEARIN DAN MINYAK IKAN LEMURU UNTUK MEMBUAT LEMAK MARGARIN [Enzymatic Interesterification of Palm Stearin and Sardine oil to Produce Margarine-fat]
Pudji Hastuti,Tyas Utami
Jurnal Teknologi dan Industri Pangan , 2003,
Abstract: Enzymatic interesterification of Palm Stearin (PS) and Sardine Oil (SO) as source of Eicosa Pentaenoic Acid (EPA) and Docosa Hexaenoic Acid (DHA) have been of interest to modify the physical properties of the triglyceride. An attempt to enzymatic-restructure PS and SO to form Structured Lipid (SL) which is suitable for margarine was investigated using immobilized lipase from Rhizomucor miehei and that from Candida antartica. The effect of reaction time course, ratio of PS/SO and ratio of enzyme/substrate were studied in the present study. At the end of interesterification, the enzyme was filtered from the reaction mixture through a filter paper. The Solid Fat Index (SFI) was determined by dillatometry. The Slip Melting Point (SMP) was determined by capillary tube method. Both of interesterification catalyzed by immobilized sn 1,3 specific lipase from R.miehei,and non specific lipase from C.antartica were found to decrease the SFI value at 10; 21.1 and 33.3°C. The SMP value was decrease from 58-50°C to 37-39°C. The change of these parameters were slightly faster in the reaction which catalyzed by lipase from R miehei than lipase from C.antartica . The more the utilization of the enzyme the faster the change were occurred, especially the increase of enzyme utilization from 2.5% to 5%, which decrease the SFI value at 33,30C. The decrease of the PS/SO ratio resulted in the decrease of SFI and SMP values. It was found that the most suitable SFI and SMP value for margarine fat is the SL formed by carrying out the enzymatic-interesterification of PS/SO with the ratio of 40/60 using enzyme 2.5% of the total fat, for 8 hours at 60°C.
Suplementasi β-glucan dari ragi roti (Saccharomyces cerevisiae) dalam pakan terhadap aktivitas fagositosis, aktivitas NBT, total protein plasma dan aktivitas aglutinasi darah ikan nila (Orechromis niloticus)
Sri Dwi Hastuti
Depik Jurnal , 2012,
Abstract: Tilapia is an important fisheries comodity which intesively farmed in Indonesia. However, an intensive farming system of aquatic organism often lead to disease outbreak. Ones the disease emerged, it will be difficult to combat. The curing method by using antibiotics in aquatic organism will bring negative impacts, such as causing bacteria resistance and decline of aquatic environments. Therefore control of disease in aquatic organism should be done through preventive methods such as the use of β-glucan from baker’s yeast (Saccharomyces cerevisiae). It is widely known that yeast’ cell wall contain of immunostimulant substance namely β-glucan. This research was aimed to find the effect of different dose of β-glucan in diet toward the phagocytic activity, respiratory burst which is indicated by Nitroblue Tetrazolium (NBT) activity, total of protein plasm and agglutination activity of tilapia blood. Method used in this study was experiment by complete random design using five treatments triplicate. Those treatments were β-glucan 0; 2,5; 5; 7,5 and 10 ppmkg-1 diet. Result showed that the different dose of β-glucan in diet gave a significantly effect on the phagocytic activity and total of protein plasm, but not on respiratory burst (NBT activity). The highest phagocytic activity occured in treatment E (β-glucan 10 ppmkg-1 diet) with the value of phagocytic activity was 37,67%, whereas the highest of total of protein plasm was occured in treatment B (β-glucan 2,5 ppmkg-1 diet) with the value of 123,58 mgml-1.
Performance of Oxfendazole in Nematode-Infected Post-Weaning Lamb on Pasture
Sri Hastuti,M Samsi
Journal of Animal Production , 2010,
Abstract: A study was conducted to determine the effectiveness of drug anthelmintic intensity oxfendazole against gastrointestinal nematode worm infections in weaned lambs. Experiments conducted on 18 lambs weaned off, randomly divided into 3 groups namely P0: grazed sheep, untreated as controls, P1: grazed sheep treated with oxfendazole, and P2: un-grazed sheep, not treated with oxfendazole. The total worm eggs per gram (TEG) in the feces until 9 weeks before treatment 9 with no significant differences (P<0.05) and up to week 12 was not significantly different (P>0.05), means that the grazing treatment oxofendale treatment have real impact on the intensity of infection at week 9 post-treatment. This is related to the maturing of young worms and the intensity of infection depends on the influence of time outside the body of sheep. Of total plasma protein (TPP) and packed cell volume (PCV) until week 12 showed that antibiotic treatment measures and grazing is very real effect (P<0.01), between the treatment group P1 with different P0 is very real (P<0,01), between P2 with different P0 very real (P<0.01) and between P1 and P2 is not significantly different (P>0.05). Means treatment with oxfendazole helminthic very real effect on total plasma protein and packed cell volume in both the shepherd and the sheep who did not shepherd. (Animal Production 12(1): 39-43 (2010)Key Words: oxfendazole, grazing and gastrointestinale nematod
Viral and Bacterial Diseases in Broiler Chicken Farms at the Area of Banyumas District
Sri Hastuti,Endro Yuwono
Journal of Animal Production , 2011,
Abstract: Efforts to control viral and bacterial diseases in poultry broiler should always be done by various efforts. Maintenance of strict management, implementation and administration of vaccines and bio security program are some efforts that are often done by farmers in order to eliminate viral and bacterial diseases. The purpose of this study was to determine the type of pathogenic viruses and bacteria that often infect broiler chickens and how to make efforts to control the broiler chicken farms in the subdistricts of Kedungbanteng and Baturraden, district of Banyumas. The research method was survey on broiler chicken farmers in the areas of Baturraden and Kedungbanteng of Banyumas district. Gradual cluster sampling was used in this study. 11 broiler chicken farms with various breeds were involved. The current study found that types of bacterial disease that infected broiler chicken farms in the subdistricts of Kedungbanteng and Baturraden were Chronic Respiratory Disease (CRD) and Colibacilosis, whereas viral diseases that infected were the Infectious Bursal Disease (IBD/Gumboro) and New Castle Disease (ND), although the prevalence rate was low. The efforts to control the main virus diseases, IBD and ND, succeeded in pressing the two diseases sould be implemented.Key Words: viral disease, bacterial disease, broiler chicken farm
Law Enforcement Against Fighting Group By Native In West Papua
Irna Indira Ratih
Pattimura Law Journal , 2016, DOI: -
Abstract: The fundamental research conducted by looking at the phenomenon of group fights and how ethnocentrism and conflict influence each other indigenous ethnic groups in West Papua. This study used descriptive qualitative method, through field studies (in-depth interviews) and literature study. The results showed that with a low level of understanding of the history of the conflict supported ever experienced by the perpetrators of communication, there is a trend of negative information transformation process to be effective. So as to absorb the negative information, aspects of ethnocentrism group members appear. The emergence of these aspects lead to the awareness and solidarity groups to join forces in-group. Other findings also showed that the factors causing conflict among indigenous ethnic groups in West Papua can be summarized in two points, namely; The first tendency puts another group at a level lower social interaction, and the second is the attempt appointment of existence itself by an ethnic group seeking recognition coupled behavior (respect) from inside and outside the group by scapegoating other groups. In the indigenous ethnic group communication, attitude and behavior aspects play a role in the formation of a negative message, based on the main factors. The tendency of formation of the group because of its frequency along the occurring hereditary. Related conflict and ethnocentrism, can be drawn that the results of the analysis found the substance also major causal relationship. Where, a communal conflict can result from the manifestation of ethnocentrism aspects into forms of behavior conflict. In contrast with the presence of conflict can also strengthen or weaken ethnocentrism.
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