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Search Results: 1 - 10 of 223917 matches for " R; Padula "
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Ecología y evolución de hantavirus en el Cono Sur de América
Murua,R; Padula,P;
Archivos de medicina veterinaria , 2004, DOI: 10.4067/S0301-732X2004000100001
Abstract: hantavirus are associated with a single primary rodent host of the familiy muridae in three sub families, two of them murinae and arvicolinae distributed in the paleartic region (europa, asia, china) and the sub family sigmodontinae in north, central and south america besides an arvicolinae genus (microtus) in north america. studies on the host and virus phylogeny show close similarities when are compared which implies that hantavirus are very ancient infectious agents which have coevolved with the rodent host. the history of earth, tectonic movements and climatic changes which affected the continents in the past are relevant to understand the host reservoir and its micro parasites current geographic distribution. this review provides historical biogeography of the sigmodontine rodents, phylogentic analyses of hantavirus, its molecular epidemiology and its geographical distribution in south america in order to sustain the proposal that the virus- rodent interaction has coevolved in the neartic before the family muridae was detached in subfamilies and before the sigmodontines rodents spread into the south american continent. it is described the existence of a large number of hantavirus lineages with small differences which make difficult to establish so far, well define species of hantavirus. an analisis between similarities and differences in the ecology and pathogenesis of two virus which have produced an important number of human cases in north america (sin nombre) and in the southern cone of america (virus andes) is discussed.
Ecología y evolución de hantavirus en el Cono Sur de América Ecology and evolution of hantavirus in the Southern Cone of America
R Murua,P Padula
Archivos de medicina veterinaria , 2004,
Abstract: Los hantavirus tienen huéspedes especie específicos pertenecientes a una familia común Muridae con tres sub familias, dos de ellas Murinae y Arvicolinae que se distribuyen en áreas geográficas de Europa Asia y Oceanía con un género Arvicolinae en América del Norte y la Sub familia Sigmodontinae en Centro América y Sudamérica. Estudios de la filogenia del huésped y el virus muestran fuertes similitudes al ser comparados, lo que sugiere una asociación de mucha más larga data con un proceso de coevolución entre el agente infeccioso y sus huéspedes roedores. La historia de la tierra y los procesos tectónicos y climáticos que afectaron al continente en épocas pretéritas son relevantes para comprender la actual distribución de los reservorios huéspedes y sus parásitos. Se entregan antecedentes biogeográficos de los roedores con la distribución geográfica de los hantavirus en Sudamérica, análisis filogenético de los virus, epidemiología molecular que sustentan la propuesta que el virus y el roedor han coevolucionado antes del momento de separarse la Familia Muridae en subfamilias (Murinae, Arvicolinae y Sigmodontinae) y anterior al ingreso de los roedores sigmodontinos al continente sudamericano. Se discute la dificultad en demarcar especie nueva de hantavirus y la existencia de varios linajes con diferencias peque as entre si para ser consideradas como especies virales. Se describen diferencias y similitudes entre las dos especies de hantavirus que más casos han producido en América del Norte (virus Sin Nombre) y en el Cono Sur de de América (Virus Andes). Hantavirus are associated with a single primary rodent host of the familiy Muridae in three sub families, two of them Murinae and Arvicolinae distributed in the Paleartic Region (Europa, Asia, China) and the sub family Sigmodontinae in North, Central and South America besides an Arvicolinae genus (Microtus) in North America. Studies on the host and virus phylogeny show close similarities when are compared which implies that hantavirus are very ancient infectious agents which have coevolved with the rodent host. The history of earth, tectonic movements and climatic changes which affected the continents in the past are relevant to understand the host reservoir and its micro parasites current geographic distribution. This review provides historical biogeography of the sigmodontine rodents, phylogentic analyses of hantavirus, its molecular epidemiology and its geographical distribution in South America in order to sustain the proposal that the virus- rodent interaction has coevolved in the Neartic before the Fa
Embalagens alternativas para café solúvel
ALVES, Rosa M. Vercelino;MILANEZ, Cibele R.;PADULA, Marisa;
Ciência e Tecnologia de Alimentos , 2000, DOI: 10.1590/S0101-20612000000200014
Abstract: the objective of this paper was to estimate the instant coffee shelf life in new options of plastic pots through a mathematic model, which relates the product moisture increase with the moisture barrier of the packaging. the plastic pots were characterized by the dimensions, the volumetric capacity, the water vapor transmission rate (wtvr) and the removal torque. the two differents types of instant coffee (aglomerated and powder) were characterized by the initial moisture and by the moisture sorption isotherm. the results presented that the plastic pots of polyester (pp) are about ten times more permeated than the polypropylene (pp) ones. the aglomeration was observed in the products with the moisture value of 7% (d.b.), but the critic moisture value was considerated 5% (d.b.), that is the tenor fixed by the legislation. the results showed that in the polypropylene (pp) pots the shelf life periods for aglomerated instant coffee and powder instant coffee was higher than 1,8 and 2,5 years, respectively, at 30oc/80%rh. however, the use of the polyester (pet) pots didn't show to be viable because of the lower shelf life periods estimated at 30oc/80%rh (lower than 4 months).
Embalagens alternativas para café solúvel
ALVES Rosa M. Vercelino,MILANEZ Cibele R.,PADULA Marisa
Ciência e Tecnologia de Alimentos , 2000,
Abstract: O objetivo deste trabalho foi estimar a vida útil de café solúvel em novas op es de potes plásticos através de modelo matemático, que relaciona o aumento de umidade do produto, com a barreira à umidade da embalagem. Os potes plásticos foram caracterizados quanto às dimens es, à capacidade volumétrica, à taxa de permeabilidade ao vapor d'água (TPVA) e ao torque de abertura. Caracterizou-se os dois tipos de café solúvel (aglomerado e em pó) quanto à umidade inicial e crítica e à isoterma de sor o de umidade. Os potes plásticos de poliéster (PET) mostraram-se cerca de dez vezes mais permeáveis que os de polipropileno (PP). Observou-se aglomera o nos produtos com 7,0% b.s. de umidade a 30oC, mas a umidade crítica foi considerada como sendo de 5,0% b.s., que é o teor fixado pela legisla o. Os resultados indicaram que, nos potes de PP, os períodos de vida útil dos cafés solúveis aglomerado e em pó foram superiores a 1,8 anos e 2,5 anos, respectivamente, a 30oC/80%UR. Entretanto, o uso de potes de poliéster (PET) n o se mostrou viável devido aos baixos períodos de vida útil estimados a 30oC/80%UR (inferiores a 4 meses).
Estratégias competitivas e de desenvolvimento de produtos lácteos funcionais: estudos de caso em empresas agroindustriais da regi?o Sul do Brasil
Lima, Mateus Silva de;Révillion, Jean Philippe Palma;Padula, Antonio Domingos;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782009005000134
Abstract: this multi-case study searched to understand why and how food processing industries develop new products and market orientates to compete in the brazilian functional dairy market. it also examines the regulatory framework role in this process. we use case studies as an approach to give evidence of the systemic relations and the existent trade-offs among factors related to innovation. we chose a research design with one unity of analysis (food processors that recently launched new products in the functional dairy market) and multiple case studies (three brazilian dairies of the southern region). the results reveal that the enterprises adopt a second-mover strategy vis-à-vis the multinational competitors. traditionally, this strategy is considered safer and requires lower r&d and marketing investments. however, it erodes branding and also limits financial returns from innovations. the second-mover behavior is consequence of poor market orientation and restricted cooperation with external partners. this "vicious circle" is also enhanced by barriers established by the regulatory framework.
Café torrado e moído em embalagens inertizadas
Alves, Rosa M. Vercelino;Mori, Emília E.;Milanez, Cibele R.;Padula, Marisa;
Ciência e Tecnologia de Alimentos , 2003, DOI: 10.1590/S0101-20612003000400005
Abstract: in this paper it was evaluated the effect of the barrier properties of packaging materials and filling conditions in vertical flow pack equipment (without and with nitrogen injection), on the shelf-life of roasted and ground coffee. three types of packaging materials made by s laminate of metallized polyester (petmet) and linear low density polyethylene (lldpe), that had a different metallization layer (otr of 0.5; 2.9 and 5.5 cm3(stp)/m2/day at 25oc, 1 atm in dry conditions) were evaluated. after formation, filling and heatsealing of the packages, the samples were stored at 25±2oc/65±3%rh. a decrease of the residual oxygen concentration of the headspace was observed during the storage, probably caused by consumption of that gas in oxidation reactions. it caused sensorial changes in the product and limited its shelf-life. the results indicated that the shelf-life to the coffee was at least 6 months at 25oc/65%rh in nitrogen gas flushing packages (2 to 3% of residual oxygen in a headspace average of 680ml at 25oc and 0.92atm), while in the system without nitrogen injection the shelf-life was 3 months. it was observed that packaging materials with oxygen transmission rates closer to the tested ones offer the same protection to roasted and ground coffee until 6 months of storage in nitrogen gas flushing packages.
Diversifica??o produtiva e as possibilidades de desenvolvimento: um estudo da fruticultura na regi?o da Campanha no RS
Rathmann, Régis;Hoff, Debora Nayar;Santos, Omar Inácio Benedetti;Padula, Ant?nio Domingos;
Revista de Economia e Sociologia Rural , 2008, DOI: 10.1590/S0103-20032008000200003
Abstract: studies indicate the existence of a situation of underdevelopment in the southern half of rio grande do sul, directly correlated with the farming and rice culture crises. the later suffers from the macroeconomic policies linked to the opening of the economy and with the pressures of the increase of competitiveness. this process has made the region present inferior performances than state averages when indicators such as gdp and per capita income are analyzed. seeking to reduce these inequalities, governmental sectors have been elaborating development strategies through the utilization of the regional agricultural vocation as well as the insertion of differentiated chains in local agribusiness. one of these experiences is the program for the development of irrigated fruit culture in the southern half/rs. within this context, this article has the objective of presenting the results observed in the gdp and hdi of two municipalities located in the campanha region of rio grande do sul, and also to present the effects observed on the life quality of the producers after the implementation of fruit culture in their properties. based on the data collected and the methodology chosen, it can be said that the productive diversification of rural properties contributes in the improvement of the producers' life quality, having positive effects on the per capita gdp and hdi of the municipalities studied.
Analysis of reporting of systematic reviews in physical therapy published in Portuguese Análise da apresenta o textual de revis es sistemáticas em fisioterapia publicadas no idioma português
Rosimeire S. Padula,Raquel S. Pires,Sandra R. Alouche,Luciana D. Chiavegato
Brazilian Journal of Physical Therapy , 2012,
Abstract: BACKGROUND: Systematic reviews are considered the best design to synthesize all existing information of a given research topic. To date, there is no study that investigated the quality of reporting of systematic reviews relevant to physical therapy published in Portuguese. Objective: To analyse the quality of reporting of systematic reviews in the field of physical therapy published in Portuguese by using the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) checklist. METHOD: All systematic reviews published in Portuguese that were indexed on PEDro database up to August 2011 were included. The quality of reporting of the eligible papers was analysed by using the PRISMA checklist. Each quality assessment was performed by two independent reviewers with arbitration of a third reviewer if necessary. RESULTS: A total of 37 systematic reviews were identified. These studies were published between 2003 and 2010. Less than 30% of the PRISMA checklist items were satisfied, being most of the items related to the introduction and discussion sections. No improvements over time were observed. CONCLUSIONS: Most of the studies did not satisfy the items from the PRISMA Checklist. It seems that most of authors did not know the existence of this checklist. The implementation of reporting statements such as the PRISMA statement by Portuguese-written journals is likely to help authors to write their systematic reviews in a more transparent and clear way. CONTEXTUALIZA O: As revis es sistemáticas s o consideradas a melhor forma de sintetizar toda a informa o existente sobre um determinado tópico, porém n o se conhece, até o momento, a qualidade da apresenta o textual das revis es sistemáticas em fisioterapia publicadas no idioma português. Objetivo: Analisar a apresenta o textual de revis es sistemáticas em fisioterapia publicadas no idioma português utilizando as recomenda es PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). MéTODO: Foram analisadas todas as revis es sistemáticas apresentadas na base de dados PEDro até o mês de agosto de 2011. Para a análise da descri o textual foi utilizada a lista de verifica o PRISMA. Cada revis o foi avaliada por pares de revisores independentes e, em caso de discordancia entre os pares, um terceiro avaliador fez a arbitragem final. RESULTADOS: Foram identificadas 37 revis es sistemáticas que foram publicadas entre os anos de 2003 e 2010. Menos de 30% dos itens da lista de verifica o PRISMA foram descritos pelos autores, sendo que a maioria dos itens satisfeitos se refere às
Deep Imaging: How Much of the Proteome Does Current Top-Down Technology Already Resolve?
Elise P. Wright, Kali A. G. Prasad, Matthew P. Padula, Jens R. Coorssen
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0086058
Abstract: Effective proteome analyses are based on interplay between resolution and detection. It had been claimed that resolution was the main factor limiting the use of two-dimensional gel electrophoresis. Improved protein detection now indicates that this is unlikely to be the case. Using a highly refined protocol, the rat brain proteome was extracted, resolved, and detected. In order to overcome the stain saturation threshold, high abundance protein species were excised from the gel following standard imaging. Gels were then imaged again using longer exposure times, enabling detection of lower abundance, less intensely stained protein species. This resulted in a significant enhancement in the detection of resolved proteins, and a slightly modified digestion protocol enabled effective identification by standard mass spectrometric methods. The data indicate that the resolution required for comprehensive proteome analyses is already available, can assess multiple samples in parallel, and preserve critical information concerning post-translational modifications. Further optimization of staining and detection methods promises additional improvements to this economical, widely accessible and effective top-down approach to proteome analysis.
Chromosomal effects of infections in malnourished and eutrophic children of Gran La Plata
Padula,G; Seoane,A;
BAG. Journal of basic and applied genetics , 2008,
Abstract: the aim of this study was to assess the induction of structural chromosomal aberrations in peripheral blood lymphocytes of malnourished and eutrophic children with bacterial infections. thirty-six infants concurrent to the hospital interzonal de agudos y crónicos dr. alejandro korn, la plata, argentina were included in this analysis; 11 infected and malnourished (im), 7 infected and eutrophic (ie) and 18 non-infected and eutrophic (ne). children aged 1-60 months. anthropometric and clinic evaluation were performed to assess nutritional condition. we scored structural chromosome aberrations (sca) in 100 metaphases per individual. statistical analysis was performed by the epi dat 3.0 (ops-oms, 2003), through ?test de diferencias entre dos proporciones muestrales? (p<0.05). total sce frequency was five times higher in im children than that of ie ones (15,1% vs. 3,33% p<0,001) and two times greater in ie than in ne children (3,33% vs. 1,88% p<0.05). results presented here showed an increase frequency of sca not only in relation with malnutrition but also with the presence of bacterial infections. it is difficult to discern whether structural chromosome aberrations are due to malnutrition per se, bacterial or viral infections, antibiotics or all of these factors acting on malnourished tissues. in conclusion, mutagenic factors cause chromosomal changes more easily in an altered environment.
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