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Search Results: 1 - 10 of 346287 matches for " R.Q. Wang "
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Anticoccidial Activity of Eupatorium adenophorum Extracts Against Chicken Coccidian Oocysts
J.F. Yang,R.Q. Wang,R.Q. Lv,D.H. Zhou,G. Duan,F.C. Zou
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.1255.1257
Abstract: The potential anticoccidial activity of Eupatorium adenophorum extracts was evaluated against coccidian oocysts which are common parasites in poultry farms. Fresh unsporulated coccidian oocysts were exposed to 2, 1.5, 1 or 0.5% of E. adenophorum extracts and the sporulation rate was measured after 72 h. A total of 80 chickens were inoculated with those attenuated oocysts. After 1 week, the birds were challenged with 1x105 untreated fresh sporulated oocysts and the survival rate, Oocyst Per Gram feces (OPG), cecum lesion score, body weight gain rate and Anticoccidal Index (ACI) were measured and calculated on the 8th day after the challenge infection. The extracts of E. adenophorum were found to be significantly effective against coccidian oocyst activity (p<0.05). These results indicate that E. adenophorum may potentially be a new alternative for effective and environmentally-friendly control of coccidiosis. The results also have important implications for the development of attenuated coccidiosis vaccine.
Fertility Status of Cultivated Land in Azad Kashmir
M. Almas,R.Q. Saeed
Pakistan Journal of Biological Sciences , 2000,
Abstract: Not Available
Prevalence of Anisakid Larvae in Maricultured Sea Fish Sold in Guangzhou, China
Q. Chen,H. Zhang,H.Q. Song,H.Q. Yu,R.Q. Lin,X.Q. Zhu
Journal of Animal and Veterinary Advances , 2012,
Abstract: The prevalence of anisakid larvae in maricultured sea fish sold in Guangzhou, China were investigated in November 2006. A total of 208 individuals representing 48 fish species were obtained from a wholesale market. They were examined for the presence of anisakid larvae by examination of the viscera and whole body cavity. Eight species of fish (16.7, 8/48), namely Pseudosciaena crocea, Muraenesox cinereus, Evynnis cardinalis, Plectorhynchus diagrammus, Scomberomorus koreanus, Plectorhynchus nigrus, Nemipterus virgatus and Siganus fuscescens, were found to be infected with anisakid larvae, with an overall infection rate of 7.7% (16/208). The results of present investigation demonstrated the risk of human infection with anisakid larvae in Guangzhou, China, which have implications for implementing control strategies against anisakid infection in human in China.
Characterisation of Angiostrongylus cantonensis Isolates from China by Sequences of Internal Transcribed Spacers of Nuclear Ribosomal DNA
C.Y. Liu,R.L. Zhang,M.X. Chen,J. Li,L. Ai,C.Y. Wu,X.Q. Zhu,R.Q. Lin
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2011.593.596
Abstract: The present study examined sequence variations in the Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) among Angiostrongylus cantonensis isolates from Shenzhen, Qingyuan, Jiangmen and Wenzhou in China. The ITS of nuclear rDNA was amplified from individual A. cantonensis by Polymerase Chain Reaction (PCR) and the representative amplicons were cloned and sequenced. The length of the ITS sequences was 1593-1614 bp for all Chinese A. cantonensis specimens and these sequences were composed of complete ITS-1 sequence of 712-720 bp, complete 5.8 S sequence of 153 bp, complete ITS-2 sequence of 633-650 bp and partial 28 S sequence of 70 bp. The intra-specifc sequence variation in A. cantonensis was 0.1-1.0% for ITS-1 and 0.0-1.3% for ITS-2 whereas sequence comparison revealed that the inter-specifc sequence differences were higher: 15.0-34.6% for ITS-1 and 22.7-24.2% for ITS-2 between A. cantonensis and other Angiostrongylus sp. The results showed that the ITS sequences were conserved among the A. cantonensis isolates however, they were quite different from that of other Angiostrongylus species. Therefore, ITS sequences could provide useful genetic markers for the specific identification and genetic characterization of Angiostrongylus sp.
Influence of Selenium on Hepatic Antioxidant Capacity in Ducklings Intoxicated with Afatoxin B1
D.Y. Shi,S.Q. Liao,R.S. Su,R.Q. Lin,Y.C. Lin,Z.X. Tang,S.N. Guo
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.1052.1055
Abstract: To investigate the influence of selenium on hepatic antioxidant capacity in ducklings administrated with Afatoxin B1 (AFB1), ninety 7 days old ducklings were randomly divided into three groups (groups I-III). Group I was used as a blank control. Group II was administered with AFB1 (0.1 mg kg-1 body weight). Group III was administered with AFB1 (0.1 mg kg-1 body weight) plus selenium (sodium selenite, 1 mg kg-1 body weight). All treatments were given once daily for 21 days. The results showed that the activity of hepatic Superoxide Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GSH-Px) and Glutathione Reductase (GR) in group II ducklings significantly decreased when compared with group I (p<0.01). Furthermore, the content of hepatic Malondialdehyde (MDA) significantly increased (p<0.01). However, the activities of hepatic SOD, CAT, GSH-Px and GR in group III ducklings significantly increased when compared with group II (p<0.05). In addition, the content of hepatic MDA significantly decreased (p<0.01). These results revealed that AFB1 significantly induced hepatic antioxidant function dysfunction. However, selenium could significantly alleviat the negative effect induced by AFB1.
Increased expression of tissue factor and protease-activated receptor-1 does not correlate with thrombosis in human lung adenocarcinoma
de Meis, E.;Azambuja, D.;Ayres-Silva, J.P.;Zamboni, M.;Pinheiro, V.R.;Levy, R.A.;Monteiro, R.Q.;
Brazilian Journal of Medical and Biological Research , 2010, DOI: 10.1590/S0100-879X2010007500017
Abstract: a correlation between cancer and hypercoagulability has been described for more than a century. patients with cancer are at increased risk for thrombotic complications and the clotting initiator protein, tissue factor (tf), is possibly involved in this process. moreover, tf may promote angiogenesis and tumor growth. in addition to tf, thrombin seems to play a relevant role in tumor biology, mainly through activation of protease-activated receptor-1 (par-1). in the present study, we prospectively studied 39 lung adenocarcinoma patients in relation to the tumor expression levels of tf and par-1 and their correlation with thrombosis outcome and survival. immunohistochemical analysis showed tf positivity in 22 patients (56%), most of them in advanced stages (iii and iv). expression of par-1 was found in 15 patients (39%), most of them also in advanced stages (iii and iv). remarkably, no correlation was observed between the expression of tf or par-1 and risk for thrombosis development. on the other hand, patients who were positive for tf or par-1 tended to have decreased long-term survival. we conclude that immunolocalization of either tf or par-1 in lung adenocarcinoma may predict a poor prognosis although lacking correlation with thrombosis outcome.
Procoagulant properties of human MV3 melanoma cells
Geaquinto, D.L.A.;Fernandes, R.S.;Lima, L.G.;Barja-Fidalgo, C.;Monteiro, R.Q.;
Brazilian Journal of Medical and Biological Research , 2008, DOI: 10.1590/S0100-879X2008005000003
Abstract: a correlation between cancer and prothrombotic states has long been described. more recently, a number of studies have focused on the procoagulant mechanisms exhibited by tumor cells. in the present study, we dissected the molecular mechanisms responsible for the procoagulant activity of mv3, a highly aggressive human melanoma cell line. it was observed that tumor cells strongly accelerate plasma coagulation as a result of: i) expression of the blood clotting initiator protein, a tissue factor, as shown by flow cytometry and functional assays (factor xa formation in the presence of cells and factor viia), and ii) direct activation of prothrombin to thrombin by cells, as evidenced by hydrolysis of the synthetic substrate, s-2238, and the natural substrate, fibrinogen. this ability was highly potentiated by the addition of exogenous factor va, which functions as a co-factor for the enzyme factor xa. in contrast, prothrombin activation was not observed when cells were previously incubated with degr-factor xa, an inactive derivative of the enzyme. moreover, a monoclonal antibody against bovine factor xa reduced the prothrombin-converting activity of tumor cells. in conclusion, the data strongly suggest that mv3 cells recruit factor xa from the culture medium, triggering an uncommon procoagulant mechanism.
Cinética de secagem de folhas de Solanum lycocarpum A. St.-Hil. (fruta-de-lobo)
Prates, M.F.O.;Reis, R.C.;Devilla, I.A.;Faria, R.Q.;Lima Junior, A.F.;
Revista Brasileira de Plantas Medicinais , 2012, DOI: 10.1590/S1516-05722012000300014
Abstract: the aims of this study were to determine the drying time and the shape of the drying curves of leaves of wolf apple (solanum lycocarpum a. st.-hil.) dried at temperatures of 40, 50 and 60oc in a thin layer fixed-bed dryer at air velocity of 0.81 m s-1, and to estimate the effective diffusion coefficient and activation energy. eight mathematical models were fit to the experimental data. the magnitude of the coefficient of determination (r2), the mean relative error (p) and the estimated standard error (se) were used to verify the fitting degree of the models. it was concluded that: a) the drying time decreased with increasing temperatures; b) the drying models of logarithm and of midilli et al. best fit to the experimental data; c) the values of the diffusion coefficient increased with the rising temperature of the drying air; d) the value of activation energy for the leaf of solanum lycocarpum a. st.-hil. was 44.60 kj mol-1.
Molecular Phylogeny and Taxonomic Status of Domestic Yak Inferred from Cytochrome B Gene Partial Sequences
S.P. Li,H. Chang,G.M. Song,G.L. Ma,H.Y. Chen,D.J. Ji,R.Q. Geng
Journal of Animal and Veterinary Advances , 2012,
Abstract: The domestic yak (Bos grunniens) is the most important domesticated bovine species in the Qinghai-Tibetan Plateau. In the present study, the cytochrome b (Cyt b) gene partial sequences (540 bp) of 27 domestic yaks (20 sequences determined in our study and 7 sequences cited in GenBank) were analyzed. Combined with the homologous fragments of other bovine Cyt b sequences in GenBank, the phylogenetic trees of Bovinae were reconstructed by Neighbor-Joining (NJ) methods with Ovis aries as outgroup. Sequence analysis showed that, among 540 sites compared for 27 domestic yaks, 9 variable sites (1.67% of 540 sites) and 5 different haplotypes were observed, showing very low mitochondrial genetic diversity in domestic yaks. The sequence divergence and divergence time between yak (Bos grunniens/Bos mutus) and American bison were 3.33% and 1.48-1.85 million years, respectively, lower and shorter than those between yak and genus Bos (Bos taurus/Bos indicus). Phylogenetic analysis showed that yak and American bison close clustered into one embranchment, while Bos taurus and Bos indicus independently clustered into another embranchment. Our results supported that the domestic yak was domesticated from a primitive yak different from the present wild yak and the yak should be classified as an independent genus Poephagus in Bovinae.
Seroprevalence of Toxoplasma gondii Infection in Slaughter Pigs in Sichuan, China
F.F. Shu,D.Y. Wu,Z.Y. Zhou,R.Q. Lv,J.F. Yang,K. Nie,G. Duan,F.C. Zou
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2011.1638.1639
Abstract: The seroprevalence of Toxoplasma gondii infection in slaughter pigs in Sichuan province, Southwestern China was investigated between May and November, 2010. A total of 803 serum samples from slaughter pigs were collected from seven different counties in Sichuan province and assayed for T. gondii antibodies by Indirect Haemagglutination (IHA) test using a commercially available kit. The overall seroprevalence was 29.51% (237/803), ranging from 9.3-37.91% among different sampling regions. The results of the present survey indicated that the seroprevalence of T. gondii infection in slaughter pigs in Sichuan province is relatively high and consumption of pork may be a risk factor for human infection with T. gondii. Therefore, improved integrated strategies for the control of the parasite should be implemented to reduce the prevalence levels in the surveyed areas.
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