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An attempt to reduce impacts of limestone quarries through biodiversity assessment and translocation: A case study at the Holcim Limestone Quarry Site in Puttalam, Sri Lanka
A. Kumarasinghe,W.A.A.D.G. Pradeep,P.I.K. Peabotuwage,R.G.A.T.S. Wickramaarachchi
Asian Journal of Conservation Biology , 2013,
Abstract: A conservation project was implemented at a commercial limestone quarry site in Sri Lanka managed by Holcim Lanka (Pvt.) Ltd. The project intended to assess the biodiversity of a proposed excavation site and to translocate fauna that will be affected by quarry operations such as forest clearance and blasting. The biodiversity of the area was surveyed using a rapid assessment technique, prior to the initiation of forest clearance and blasting. A total of 41 floral species and 220 faunal species were recorded from the project site. Around 90 % of the fauna were amphibians, reptiles and butterflies. Among these species, one endemic tree, a theraposid spider and 20 endemic vertebrates. Among the vertebrates documented, 9 species are categorized as nationally threatened. A total of 141 vertebrates and 85 arthropods and mollusks including endemics threatened species were captured and translocated to Sethtavilluwa area. This project is the first ever initiative in Sri Lanka aimed at reducing impacts of quarry operation on biota through rehabilitation and rescue operations. Such projects are invaluable as they will, at least in part assist in safeguarding biota that will be vulnerable to local extinction as a result of developmental projects.
Molecular Detection and Partial Characterization of Tomato Yellow Leaf Curl Virus in Sri Lanka
S.A.M.C. Samarakoon,A. Balasuriya,R.G.A.S. Rajapaksha,W.A.R.T. Wickramarachchi
Pakistan Journal of Biological Sciences , 2012,
Abstract: Tomato Yellow Leaf Curl Virus (TYLCV) is an important plant virus on one of the economically most important vegetable crops; tomato (Lycopersicon esculentum Mill.). This had not been molecularly detected before, in Sri Lanka. TYLCV-GN-SL was isolated from apparently infected tomato plants using modified Cetyltrimethyl Ammonium Bromide (CTAB) method in Gannoruwa. Associated Begomoviruses were detected using Deng 541/Deng 540 and AV 494/AC 1048 primer pairs. TYLCV was detected for the first time in tomato in Sri Lanka using P1V/P4C, TYLCV specific primer pair. Nucleotide sequence of coat protein of isolated TYLCV-GN-SL proved that the Indian strain of ToLC virus was closely related to Tomato Leaf Curl Sri Lanka Virus (TLCV-SL: 97%) and Tomato leaf curl Geminivirus (TLCGV: 93%) through direct sequencing data. TLCV-SL was confirmed as TYLCV isolate. TYLCV was molecularly detected from major tomato growing districts like Badulla, Nuwara-Eliya, Kandy and Matale in Sri Lanka.
Lactation curve of cross-bred buffalo under two production systems in the Amazonian region of Brazil
S.B.P. Barbosa,R.G.A. Pereira,K.R. Santoro,A.M.V. Batista
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2007.s2.1075
Abstract: This study was conducted at the Agroforestry Research Center of EMBRAPA (CPAFRO), Rond nia, Brazil, to evaluate the lactation curve of crosss-bred buffalo [Murrah (M), Mediterranean (Me) and Jafarabadi(J)] under two production systems. Production system one (PS1) corresponded to the period of 1984 to 1998 where animals (4471 observation) were milked once a day and received only pasture without supplementation. Production system two (PS2) corresponded to the period of 1999 to 2002 where animals (458 observations) were milked twice daily with concentrate supplementation to pasture. Eight mathematical functions were used: Inverse Polynomial, Linear Hyperbolic, Incomplete Gamma, Logarithmic, Logarithmic Quadratic, Linear, Quadratic and Jenkins & Ferrel. Statistical analysis was conducted using PROC NLIN of SAS (2005). Results showed that for both production systems, Incomplete Gama was the best function to describe the lactation curve. Values of coefficient of determination, standard-deviation, coefficient of variation and standard-error were 95%, 0.068, 7.20, and 0.003, respectively. The corresponding values for PS2 were 96%, 0.200, 2.12, and 0.003. Values of the lactation curve parameters (a, b, and c) for PS1 and PS2, respectively, were (a) 7.0035923 and 10.9209, (b) -0.1080043 and -0.1614882, (c) 0.0434868 and 0.0679365.
Milk yield of cross-bred buffalo under two production systems in the Amazonian region of Brazil
S.B.P. Barbosa,R.G.A. Pereira,K.R. Santoro,A.M.V. Batista
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2007.s2.1071
Abstract: This study was conducted at the Agroforestry Research Center of EMBRAPA (CPAFRO), Rond nia, Brazil, to determine the effects of various factors on milk yield (MY) of crosss-bred buffalo (Murrah, Mediterranean and Jafarabadi) under two production systems. Production system one (PS1) corresponded to the period from 1984 to 1998 where animals (4471 observation) were milked once a day and received only pasture without supplementation. Production system two (PS2) corresponded to the period from 1999 to 2002 where animals (458 observations) were milked once or twice daily with concentrate supplementation to pasture. Fixed variables were contemporary group (CG), genetic group (GG), Sire (S) and Dam (D). Age (days) at calving (AC), weight at calving (WC) and tast day (TD) were included as linear covariates. Data were analyzed using PROC GLM (SAS, 2005). Results showed that average milk yield for PS1 and PS2 were 3.24 and 4.31 kg/day, respectively. With the exception of WC, in PS2, all other factors significantly affected milk yield in buffalo on two production systems. In PS1, the highest milk yield was for the genetic group 7/8M and the lowest for genetic group 1/2M. For PS2, the highest milk yield was for genetic group 3/4M and the lowest for genetic group 7/8M. Using the significant covariates, the following regression equations were developed to estimate milk yield for buffalo under the two production systems: MY(PS1) = 1.99719578 + 0.00409672WC - 0.00012372AC - 0.00616303DC; MY(PS2) = 3.650977 + 0.000462760AC - 0.010300571DC.
The protective effect of cilostazol on isolated rabbit femoral arteries under conditions of ischemia and reperfusion: the role of the nitric oxide pathway
Santos, Mariana R.G.A.;Celotto, Andréa C.;Capellini, Verena K.;Evora, Paulo R. B.;Piccinato, Carlos E.;Joviliano, Edwaldo E.;
Clinics , 2012, DOI: 10.6061/clinics/2012(02)13
Abstract: objectives: the clinical significance of ischemia/reperfusion of the lower extremities demands further investigation to enable the development of more effective therapeutic alternatives. this study investigated the changes in the vascular reactivity of the rabbit femoral artery and nitric oxide metabolites under partial ischemia/ reperfusion conditions following cilostazol administration. methods: ischemia was induced using infrarenal aortic clamping. the animals were randomly divided into seven groups: control 90 minutes, ischemia/reperfusion 90/60 minutes, control 120 minutes, ischemia/reperfusion 120/90 minutes, cilostazol, cilostazol before ischemia/reperfusion 120/90 minutes, and ischemia 120 minutes/cilostazol/ reperfusion 90 minutes. dose-response curves for sodium nitroprusside, acetylcholine, and the calcium ionophore a23187 were obtained in isolated femoral arteries. the levels of nitrites and nitrates in the plasma and skeletal muscle were determined using chemiluminescence. results: acetylcholine-and a23187-induced relaxation was reduced in the ischemia/reperfusion 120/90 group, and treatment with cilostazol partially prevented this ischemia/reperfusion-induced endothelium impairment. only cilostazol treatment increased plasma levels of nitrites and nitrates. an elevation in the levels of nitrites and nitrates was observed in muscle tissues in the ischemia/reperfusion 120/90, cilostazol/ischemia/reperfusion, and ischemia/ cilostazol/reperfusion groups. conclusion: hind limb ischemia/reperfusion yielded an impaired endothelium-dependent relaxation of the femoral artery. furthermore, cilostazol administration prior to ischemia exerted a protective effect on endotheliumdependent vascular reactivity under ischemia/reperfusion conditions.
Detec??o da resistência de capim-arroz (Echinochloa sp.) ao herbicida quinclorac em regi?es orizícolas do sul do Brasil
Andres, A.;Concen?o, G.;Melo, P.T.B.S.;Schmidt, M.;Resende, R.G.;
Planta Daninha , 2007, DOI: 10.1590/S0100-83582007000100025
Abstract: the monitoring of echinochloa sp. plants resistant to quinclorac was conducted in rio grande do sul and santa catarina, brazil to determine the origin of resistance and its dissemination, as well as to detect practices or climatic conditions related to the selection and geographic distribution of the resistant biotype. the seeds were collected, purified and standardized, and trials conducted under growth chamber and greenhouse conditions. in the growth chamber, a standard germination test was conducted with the seeds being submitted to rates of 0x, 1x, 2x, 6x, 16x and 32x and to the recommended rate of quinclorac, followed by germination curve and seedlings survival evaluation 14 days after sowing (das); the same herbicide rates were evaluated under greenhouse conditions, applied over the plants 20 days after emergence (dae). dry weight and control were evaluated 35 dae, and biotypes with ri above four were considered resistant. a map of both states was elaborated where distribution of resistance to quinclorac was showed graphically, followed by herbicide profoxydim test as an alternative for the resistant biotype control.
Total intravenous anaesthesia with propofol-racemic ketamine and propofol-S-ketamine: a comparative study and haemodynamic evaluation in dogs undergoing ovariohysterectomy
Intelisano, Tatiana R.;Kitahara, Flávia R.;Otsuki, Denise A.;Fantoni, Denise T.;Auler Jr, José O.C.;Cortopassi, Sílvia R.G.;
Pesquisa Veterinária Brasileira , 2008, DOI: 10.1590/S0100-736X2008000400004
Abstract: total intravenous anaesthesia (tiva) with propofol and ketamine proved to be very satisfactory from a clinical point of view. this blind randomised controlled trial was designed to compare induction and maintenance of anaesthesia under continuous infusion of propofol-racemic ketamine (prk) with that of propofol-s-ketamine (psk) and evaluate their haemodynamic, metabolic and ventilatory effects. seven female dogs undergoing ovariohysterectomy were involved in each group. anaesthesia was induced: in group prk, with propofol (4.0mg kg-1) and racemic ketamine (2.0mg kg-1) intravenous (i.v.), followed by i.v. infusion of propofol (initial dose of 0.5mg kg-1 min-1) and racemic ketamine (0.2mg kg-1 min-1); in group psk, with propofol (4.0mg kg-1) and s-ketamine (1.0 mg kg1) i.v., followed by i.v. infusion of propofol (initial dose of 0.5mg kg-1 min-1) and s-ketamine (0.1mg kg-1 min-1). parameters were assessed before anaesthesia and in 6 time points after induction. in both groups, heart rate increased significantly at all time points. there was a slight decrease in systemic blood pressure, cardiac output and cardiac index in both groups. the systolic index decrease significantly and intense respiratory depression was observed in all groups, making assisted ventilation necessary.
Potencial forrageiro de cultivares de girassol produzidos na safrinha para ensilagem
Tomich, T.R.;Rodrigues, J.A.S.;Gon?alves, L.C.;Tomich, R.G.P.;Carvalho, A.U.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2003, DOI: 10.1590/S0102-09352003000600013
Abstract: the objective of this study was to evaluate some important agronomic traits for silage production of 12 hybrids and a variety of sunflower (helianthus annuus l.) participant of the national assay of sunflower, in the agricultural year of 1996/97. the experiment was conducted from february to june 1997. the experimental procedure followed a randomized block design and the means values were compared by student-newman-keuls test. significant differences among hybrids for fresh and dry matter productions, leaf, head and stem percentages, stem's circumference close the head, broken plants percentage and height of the plants were observed. no significant differences among hybrids for stem's circumference close the ground, diameter of the head and laying plants percentage were found. the agronomic traits indicated that some hybrids of sunflower present potential to be used for silage production in double-cropping system, or during the drought.
COMPOSI??O, PERFIL NITROGENADO E CARACTERíSTICAS DO LEITE CAPRINO (SAANEN): REGI?O SUDESTE, BRASIL
PRATA, L.F.;RIBEIRO, A.C.;REZENDE, K.T.;CARVALHO, M.R.B.;RIBEIRO, S.D.A.;COSTA, R.G.;
Ciência e Tecnologia de Alimentos , 1998, DOI: 10.1590/S0101-20611998000400014
Abstract: during a year, were collected and analysed 179 samples of milk from three goat?s herds, having between 44 and 56 milking animals, determining their chemical composition, their mainly nitrogen fractions and their physico-chemical properties. the chemical composition was: 3,27% for total nitrogen content, 3,74% for fat, 4,35% for lactose, 0,74% for ashes and 88,49% for water content. nitrogen fractions were: 2,97% for true protein (tp), 2,43% for casein and 0,84% for serum proteins including 0,30% for non protein nitrogen matter. the values for physico-chemical properties or milk characteristics were: 1,0324 for density at 15oc, 6,65 for ph at 25oc, 16,11od for acidity, 0,172g% for chloride content, -0,574oh for freezing point, 11,51% for total solids and 7,77% solids not-fat.
Assessing wear and surface roughness of different composite resins after toothbrushing
Chimello, D.T.;Dibb, R.G.P.;Corona, S.A.M.;Lara, E.H.G.;
Materials Research , 2001, DOI: 10.1590/S1516-14392001000400011
Abstract: the aim of this work was to compare the in vitro wear and roughness of different composite resins after toothbrushing. six resins were tested: revolution (kerr), natural flow (dfl), flow it! (jeneric-pentron), fill magic flow (vigodent) - flowable composites, silux plus (3m) - microfilled composite, and z100 (3m) - hybrid composite. eight disks were prepared for each group (n = 48), with 12 mm in diameter and 1mm thick. the specimens were stored in distilled water at 37 °c for 7 days, polished (super snap), weighed and submitted to the initial roughness test. each sample was fixed on plexiglass plates and subjected to simulated toothbrushing. after abrasion, the samples were removed from the plates, weighed and submitted to the post-abrasion roughness test. statistical analysis was performed by using anova and tukey's test. pearson's test was used to verify correlation between wear and roughness. data showed a similar resistance to wear of natural flow and z100, both presenting minimum mass loss and surface roughness. silux plus presented the roughest surface after toothbrushing.
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