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Search Results: 1 - 10 of 223838 matches for " R. Saghiri "
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Evaluation of Serum Levels of Essential Trace Elements in Patients with Pulmonary Tuberculosis Before and After Treatment by Age and Gender
F. Pourfallah,S. Javadian,Z. Zamani,R. Saghiri
Pakistan Journal of Biological Sciences , 2011,
Abstract: The purpose of this study was to evaluate the levels of Zinc, Copper, Iron and Copper/Zinc ratio in the serum of adult patients with pulmonary tuberculosis in Iran. Serum levels of Zinc and Copper were determined by flame atomic absorption spectrophotometer and serum iron concentration was measured by using an Auto Analyzer. The study group consisted of 50 pulmonary tuberculosis patients before treatment and after 6 months of anti-tubercular therapy. Levels of serum Zn (p<0.001) and Fe (p<0.001) in TB patients were significantly increased after 6 months of anti-tubercular therapy. However, serum Cu concentration (p<0.01) and Cu/Zn ratio (p<0.05) were decreased after 6 months of anti-tubercular therapy. Some studies indicated a strong association of Zn, Cu, Fe and the Cu/Zn ratio with TB. In this study, we found remarkable change in Cu/Zn ratio. Some researchers mentioned that serum Cu/Zn ratio could be used as an important laboratory marker for diagnosis and treatment of tuberculosis. They also mentioned that trace element levels must be closely monitored during the process of disease.
Evaluation of Serum Levels of Zinc, Copper, Iron, and Zinc/Copper Ratio in Cutaneous Leishmaniasis
F Pourfallah,S Javadian,Z Zamani,R Saghiri
Iranian Journal of Arthropod-Borne Diseases , 2009,
Abstract: "nBackground: The purpose of this study was to evaluate the levels of zinc (Zn), copper (Cu), iron (Fe) and zinc/ cop-per ratio in the serum of patients with cutaneous leishmaniasis in Qom Province, center of Iran."nMethods: Serum levels of zinc and copper were determined by flame atomic absorption spectrophotometer and serum iron concentration was measured by using an Auto Analyzer. The study group consisted of 60 patients with cutaneous leishmaniasis and the control group of 100 healthy volunteers from the same area who were not exposed to cutane-ous leishmaniasis."nResult: There were no statistically significant differences in age and body mass index between the two groups. Se-rum Zn (P< 0.001) and Fe (P< 0.05) levels were lower in patients with cutaneous leishmaniasis than the control group. We also found serum Cu concentration (P< 0.05) in the patient group was significantly higher than that of the control group. However, zinc/ copper ratio (P< 0.001) was lower in patients with cutaneous leishmaniasis than in the control group."nConclusion: Our data indicated that Zn/Cu ratio was significantly lower in patients with CL as compared to the con-trols. Earlier reports suggest that, this ratio imbalance could be a useful marker for immune dysfunction in leishma-niasis. There was also strong association of Zn, Cu and Fe with CL. It suggests the use of blood zinc, copper, iron concentration and the copper/zinc ratio (Zn/Cu), as a means for estimating the prognosis of CL. Keywords: Cutaneous leishmaniasis, Zn, Cu, Fe. Zn/Cu ratio, Iran
Evidences on the existence of a new potassium channel in the rough endoplasmic reticulum (RER) of rat hepatocytes
Sajjad Salari,AfsanehAfsaneh Eliassi,Reza Saghiri
Physiology and Pharmacology , 2011,
Abstract: Introduction: we have recently reported the presence of two potassium currents with 598 and 368 pS conductance in the rough endoplasmic reticulum (RER) membrane. The 598 pS channel was voltage dependent and ATP sensitive. However, the 368 pS channel was rarely observed and its identity remained obscure. Since cationic channels in intracellular organelles such as mitochondria and RER play important roles in intracellular signaling and cellular protection, studying their biophysical and pharmacological properties is important. Methods: we used channel incorporation into bilayer lipid membrane method. L-α-Phosphatidylcholine (PC), a membrane lipid, was extracted from fresh egg yolk. Bilayer lipid membrane was formed in a 150 μm diameter hole. Rough endoplasmic reticulum vesicles were obtained from liver after homogenization and several centrifugations. All recordings were filtered at 1 kHz and stored at a sampling rate of 10 kHz for offline analysis by PClamp9. Statistical analysis was performed based on Markov noise free single channel analysis. Results: A 364 pS potassium channel was identified which was voltage independent in +50 to -50 mV voltages. In all voltages, open probability (Po) was over 0.9. Nonspecific inhibitor of K+ channels, 4-aminopyridine (4-AP, 20 mM), inhibited the channel activity. However, intracellular nucleotide like ATP had no effect on channel gating. Conclusion: We observed a new potassium channel with 346 pS conductance in RER membrane that unlike the 598 pS K channel was not ATP sensitive. This channel may play an important role in Ca2+ homeostasis and cell protection.
Characterization of biophysical properties of single chloride channel in rat brain mitochondrial inner membrane by channel incorporation into bilayer lipid membrane
Javad Fahanik babaei,Afsaneh Eliassi,Reza Saghiri
Physiology and Pharmacology , 2011,
Abstract: Introduction: Recent studies have shown the presence of Cl- channels in heart and liver mitochondrial membranes. In this work, we have characterized the functional profile of a Cl- channel from rat brain mitochondria. Methods: After removing and homogenizing the rat brain, the supernatant was separately centrifuged in MSEdigitonin, H2O and Na2CO3 and mitochondrial inner membrane vesicles were obtained in MSE solution. L-α- Phosphatidylcholine (membrane lipid) was extracted from fresh egg yolk. Bilayer lipid membranes were formed in a 150 μm diameter hole. All recordings were filtered at 1 kHz and stored at a sampling rate of 10 kHz for offline analysis by PClamp10. Statistical analysis was performed based on Markov noise free single channel analysis. Results: Brain mitochondrial inner membrane preparations were subjected to SDS-PAGE analysis and channel protein reconstitution into planar lipid bilayers. Western blotting and antibodies directed against various cellular proteins revealed that the mitochondrial inner membrane fractions did not contain specific proteins of the other subcellular compartments except a very small fraction of endoplasmic reticulum. Channel incorporation into the planar lipid bilayers revealed an anion selective channel with a conductance of 301 pS in 200 mM KCl cis/50 mM KCl trans. The channel open probability appeared to be voltage dependent and the channel was active between the voltages of -40 and +20 mV. Adding 10 μM DIDS to the side corresponding to the cell internal medium caused a strong inhibition of the channel activity. Conclusion: This channel is likely to be involved in maintaining proper pH, membrane potential, ATP synthesis, and cell protection.
Biophysical and electropharmacological properties of single mitoKATP channel in rat brain mitochondrial inner membrane
Javad Fahanik babaei,Afsaneh Eliassi,Reza Saghiri
Physiology and Pharmacology , 2012,
Abstract: Introduction: Different ATP-sensitive potassium channels have been detected in the mitochondrial inner membrane of cells. They are suggested to be involved in cell processes including cell protection. Here, we characterized the biophysical and electropharmacological properties of a KATP channel in the brain mitochondrial inner membranes. Methods: After removing and homogenizing the rat brain, the supernatant was separately centrifuged in MSE digitonin, H2O and Na2CO3 and mitochondrial inner membrane vesicles were obtained in MSE solution. L-α- Phosphatidylcholine (membrane lipid) was extracted from fresh egg yolk. Bilayer lipid membranes were formed in a 150 μm diameter hole. All recordings were filtered at 1 kHz and stored at a sampling rate of 10 kHz for offline analysis by PClamp10. Results: Single channel recordings revealed a channel with a slope conductance of 143 ± 7pS in voltage dependent 200 mM KCl cis/50 mM KCl trans. The closed dwell time distributions indicated one and at least two exponential components at positive and negative potentials, respectively. The open dwell time events were fitted to two exponential functions. The block by ATP and glibenclamide characterized this channel as the KATP channel. We also showed that 4- AP and TEA inhibited the channel activities. The activity of channel was not influenced by iberiotoxin, a BK channel blocker, and 5-hydroxydecanoic acid, an inhibitor of mitochondrial ATP-sensitive potassium channels. Western blotting with antibodies directed against Kir6.1 and SUR2B subunits recognized a blocking-peptide-sensitive with a molecular mass of ~55-64kDa and ~120kDa, respectively. Conclusion: This channel is likely to be involved in maintaining proper homeostasis in brain mitochondria and cell processes.
Dielectronic Recombination (via N=2 --> N'=2 Core Excitations) and Radiative Recombination of Fe XX: Laboratory Measurements and Theoretical Calculations
D. W. Savin,E. Behar,S. M. Kahn,G. Gwinner,A. A. Saghiri,M. Schmitt,M. Grieser,R. Repnow,D. Schwalm,A. Wolf,T. Bartsch,A. Mueller,S. Schippers,N. R. Badnell,M. H. Chen,T. W. Gorczyca
Physics , 2001, DOI: 10.1086/323388
Abstract: We have measured the resonance strengths and energies for dielectronic recombination (DR) of Fe XX forming Fe XIX via N=2 --> N'=2 (Delta_N=0) core excitations. We have also calculated the DR resonance strengths and energies using AUTOSTRUCTURE, HULLAC, MCDF, and R-matrix methods, four different state-of-the-art theoretical techniques. On average the theoretical resonance strengths agree to within <~10% with experiment. However, the 1 sigma standard deviation for the ratios of the theoretical-to-experimental resonance strengths is >~30% which is significantly larger than the estimated relative experimental uncertainty of <~10%. This suggests that similar errors exist in the calculated level populations and line emission spectrum of the recombined ion. We confirm that theoretical methods based on inverse-photoionization calculations (e.g., undamped R-matrix methods) will severely overestimate the strength of the DR process unless they include the effects of radiation damping. We also find that the coupling between the DR and radiative recombination (RR) channels is small. We have used our experimental and theoretical results to produce Maxwellian-averaged rate coefficients for Delta_N=0 DR of Fe XX. For kT>~1 eV, which includes the predicted formation temperatures for Fe XX in an optically thin, low-density photoionized plasma with cosmic abundances, our experimental and theoretical results are in good agreement. We have also used our R-matrix results, topped off using AUTOSTRUCTURE for RR into J>=25 levels, to calculate the rate coefficient for RR of Fe XX. Our RR results are in good agreement with previously published calculations.
Dielectronic Recombination in Photoionized Gas. II. Laboratory Measurements for Fe XVIII and Fe XIX
D. W. Savin,S. M. Kahn,J. Linkemann,A. A. Saghiri,M. Schmitt,M. Grieser,R. Repnow,D. Schwalm,A. Wolf,T. Bartsch,C. Brandau,A. Hoffknecht,A. Mueller,S. Schippers,M. H. Chen,N. R. Badnell
Physics , 1999, DOI: 10.1086/313247
Abstract: In photoionized gases with cosmic abundances, dielectronic recombination (DR) proceeds primarily via nlj --> nl'j' core excitations (Dn=0 DR). We have measured the resonance strengths and energies for Fe XVIII to Fe XVII and Fe XIX to Fe XVIII Dn=0 DR. Using our measurements, we have calculated the Fe XVIII and Fe XIX Dn=0 DR DR rate coefficients. Significant discrepancies exist between our inferred rates and those of published calculations. These calculations overestimate the DR rates by factors of ~2 or underestimate it by factors of ~2 to orders of magnitude, but none are in good agreement with our results. Almost all published DR rates for modeling cosmic plasmas are computed using the same theoretical techniques as the above-mentioned calculations. Hence, our measurements call into question all theoretical Dn=0 DR rates used for ionization balance calculations of cosmic plasmas. At temperatures where the Fe XVIII and Fe XIX fractional abundances are predicted to peak in photoionized gases of cosmic abundances, the theoretical rates underestimate the Fe XVIII DR rate by a factor of ~2 and overestimate the Fe XIX DR rate by a factor of ~1.6. We have carried out new multiconfiguration Dirac-Fock and multiconfiguration Breit-Pauli calculations which agree with our measured resonance strengths and rate coefficients to within typically better than <~30%. We provide a fit to our inferred rate coefficients for use in plasma modeling. Using our DR measurements, we infer a factor of ~2 error in the Fe XX through Fe XXIV Dn=0 DR rates. We investigate the effects of this estimated error for the well-known thermal instability of photoionized gas. We find that errors in these rates cannot remove the instability, but they do dramatically affect the range in parameter space over which it forms.
Dielectronic Recombination of Ground-State and Metastable Li+ Ions
A. A. Saghiri,J. Linkemann,M. Schmitt,D. Schwalm,A. Wolf,T. Bartsch,A. Hoffknecht,A. Mueller,W. G. Graham,A. D. Price,N. R. Badnell,T. W. Gorczyca,J. A. Tanis
Physics , 1999, DOI: 10.1103/PhysRevA.60.R3350
Abstract: Dielectronic recombination has been investigated for Delta-n = 1 resonances of ground-state Li+(1s^2) and for Delta-n = 0 resonances of metastable Li+(1s2s ^3S). The ground-state spectrum shows three prominent transitions between 53 and 64 eV, while the metastable spectrum exhibits many transitions with energies < 3.2 eV. Reasonably good agreement of R-matrix, LS coupling calculations with the measured recombination rate coefficient is obtained. The time dependence of the recombination rate yields a radiative lifetime of 52.2 +- 5.0 s for the 2 ^3S level of Li+.
Comparison of rat connective tissue reaction to two types of foreign and Iranian white Mineral Trioxide Aggregate
Vosough Hosseini S.,Lotfi M.,Saghiri MA.,Fatemi A.
Journal of Dental Medicine , 2008,
Abstract: "nBackground and Aim: Three Dimensional obturation of root canal is one of the main goals of root canal therapy to preserve health or reach the regeneration or healing of periapical tissues. Root end filling materials are used in numerous situations to reach the mentioned goals. One of the common root end- filling materials is mineral trioxide aggregate (MTA) which the foreign and Iranian ones are different in their prices. The aim of this study was to compare the rat connective tissue reaction to Iranian and foreign MTA. "nMaterials and Methods: This was an animal study in which 40 rats were divided into 5 groups of each 8. The polyethylene tubes filled with foreign (Pro Root MTA) and Iranian (Root MTA) white MTA and were implanted in subcutaneous connective tissue. Similarly, the empty tubes were inserted in subcutaneous connective tissue as control group. The samples were examined histologically after 7, 14, 30, 60 and 90 days and were scored as followings: 0, was characterized to samples without inflammatory cells; without inflammatory reaction 1, for samples with less than 25 inflammatory cells; mild inflammatory reaction. 2, for samples with 25 to 125 inflammatory cells; moderate inflammatory reaction and 3, for ones with more than 125 inflammatory cells; severe inflammatory reaction. The data were analyzed using Kruskal-Wallis test and p<0.05 was considered as the level of significance. "nResults: In general, inflammatory reactions were reduced in all groups. Experimental groups had moderate to severe inflammation in the 7th day which had significant difference with the control group having mild to moderate inflammation (p=0.04). There was not any significant differences between experimental and control group in 14th, 30th, 60th and 90th days (p>0.05). "nConclusion: Based on the findings of this investigation, inflammatory subcutaneous connective tissue reaction to Iranian (Root MTA) and foreign (Pro Root MTA) MTA was the same.
A Comparative Study of Measurement Accuracy of Cyber Space Analysis Software with Manual Method in Mixed Dentition
Sheibani Nia A.,Namvar B.,Saghiri MA.,Emami S.
Journal of Dentistry , 2011,
Abstract: Statement of Problem: One of the considerations regarding the space analysis in study casts is the issue of speed and precision in analysis.Purpose: This research aimed to design a software to conduct space analysis and evaluate its accuracy compared to manual space analysis.Materials and Method: This reasearch was conducted in two stages: Exploratory and Cross- Sectional. The subjects were selected randomly from patients between 7 to 11 years of age referring to the orthodontics clinic at the dentistry school of the Islamic Azad University. About 30 study models (15 pairs) were randomly selected. Space analysis with manual method was performed .Space analysis with the aid of Cyber Space Analysis software was also carried out by locating the required landmarks on digital images of dental casts. The accuracy of this software in comparison with the manual method in space analysis for mixed dentition was evaluated using T student test.Results: The average time required for space analysis using the software was 3.4 minutes as compared to the manual method which took 7.81 minutes on average. The results from software-assisted space analysis showed no significant difference with that obtained from the manual method ( p < 0.0001).Conclusion: The results from software-assisted space analysis were similar to those of the manual method. However, software-assisted space analysis is carried out more quickly than the manual method.
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