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Search Results: 1 - 10 of 224175 matches for " R. Felice "
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Detection of New Genomic Landmarks in the Maltese Goat Using Rapd PCR
R. Blundell,A.E. Felice
Journal of Animal and Veterinary Advances , 2012,
Abstract: Since no information of the Maltese goat genome is available, RAPD technique has been used to identify a number of DNA landmarks. Genome Landmarks have been obtained from the DNA of 66 Maltese goats which were studied with Random Amplification of Polymorphic DNA (RAPD). Eleven (11) reproducible RAPD polymorphic zones were identified. For sequencing, the RAPD zones were cloned into the Puc 18 vector utilising E. coli and then sequenced using both the forward (universal) and reverse primers specific for the Puc 18 vector. After sequencing a total of nine new markers (AF078170, AF078171, AF078172, AF078173, AF078174, AF078175, AF078176, AF078177, AF078178) amounting to approximately 5,500 bp of sequence from the goat genome were identified and their homologies with known nucleic acid and protein databases were described. The new sequences could provide useful anchors for more extensive mapping and sequencing and for genomics assisted breeding of the Maltese goat.
R Mercogliano,A De Felice,C Chirollo,R Marrone
Italian Journal of Food Safety , 2010, DOI: 10.4081/ijfs.2010.8.45
Abstract: Polycyclic aromatic hydrocarbons (PAHs), mainly formed by anthropogenic activities, are ubiquitous environmental contaminants. Due to environmental contamination and their chemical properties they migrate through the human food chain. Aim of this study was the evaluation of PAHs in ricotta and mozzarella di bufala cheese, produced by milk of buffalo collected in three farms, located in a high contaminated area in Campania because of a waste treatment plant and illegal waste incineration. 11 PAHs were identified both in milk and dairy products. Carcinogenic hydrocarbon benzo(a)pyrene were found in a range including 0.42- 12.96 μg/kg and dibenzo(ah)anthracene 0.21-10.08 μg/kg. Anthracene showed higher concentrations than the other PAHs (45.23-436.85 μg/kg).
R. Mercogliano,R. D’Ambrosio,A. De Felice,C. Chirollo
Italian Journal of Food Safety , 2008, DOI: 10.4081/ijfs.2008.2.45
Abstract: “Colatura d’alici”is a speciality made in Cetara, a clear ,amber-coloured liquid that has sharp taste, prepared with the juice coming from the anchovy salting process. The anchovies are caught in the Gulf of Salerno between the mounths of March and July. The colatura is ready at the beginning of December. Histamine poisoning, a food-borne chemical intoxication caused by the consumption of food containing toxic levels of histamine, is a syndrome commonly associated with the consumption of seafood beloging to the Engraulidae and some fish families. Histamine and biogenic amine levels were determined in “colatura di alici di Cetara”. The amine was separated in a HPLC/FL system. Histamine level were lower than the law limits only in one of the examinated products. A reduction of the histamine and amine levels in colatura cetarese can be obtained using a determinate salt concentrations and changing way of seasoning.
Telomere shortening may be associated with human keloids
Bruna De Felice, Robert R Wilson, Massimo Nacca
BMC Medical Genetics , 2009, DOI: 10.1186/1471-2350-10-110
Abstract: We analyzed sample tissues were obtained from 20 patients with keloid skin lesions and normal skin was obtained from 20 healthy donors. The telomeres were measured by Terminal Restriction Fragment (TRF) analysis and Real-Time PCR assay. Quantitative Real-Time RT-PCR analysis of hTERT gene expression was performed and intracellular ROS generation was measured.In this study, we determined whether telomeric shortening and the expression of human telomerase reverse transcriptase (hTERT) occurs in keloid patients. Using Terminal Restriction Fragment (TRF) analysis and Real-Time PCR assay, we detected a significant telomere shortening of 30% in keloid specimens compared to normal skin. Using quantitative Real-Time RT-PCR, telomerase activity was found absent in the keloid tissues. Moreover, an increase in ROS generation was detected in fibroblasts cell cultures from keloid specimens as more time elapsed compared to fibroblasts from normal skin.Telomere shortening has been reported in several metabolic and cardiovascular diseases. We found that telomere shortening can also be associated with human keloids. Chronic oxidative stress plays a major role in the pathophysiology of several chronic inflammatory diseases. Here we found increased ROS generation in fibroblasts from keloid fibroblasts cell cultures when compared to normal skin fibroblasts. Hence we conclude that oxidative stress might be an important modulator of telomere loss in keloid because of the absence of active telomerase that counteracts telomere shortening.Keloids (OMIM 148100) are benign skin tumors occurring during wound healing in genetically predisposed patients. The pattern of inheritance observed in 14 pedigrees with familial keloids has been consistent with an autosomal dominant mode with incomplete clinical penetrance and variable expression [1]. Keloids are characterized by the proliferation of dermal fibroblasts, overproduction of extracellular matrix components (ECM), an increased infiltration of
R. Mercogliano,A. De Felice,G. Pezone,V. Raffone
Italian Journal of Food Safety , 2011, DOI: 10.4081/ijfs.2011.1s.67
Abstract: Polycyclic aromatic hydrocarbons (PAHs), mainly formed by anthropogenic activities, are ubiquitous environmental contaminants. Due to environmental contamination and their chemical properties they migrate through the human food chain, and can be transferred from mother to infant via breastfeeding. The presence of PAHs in breast milk collected from 16 lactating women living in urban and rural areas of Campania (Italy) was investigated. Results showed the presence of 14 hydrocabons. Carcinogenic benzo(a)pyrene and potential carcinogenic dibenzo(ah)anthracene were found at concentrations rancing from 0.3 to 37.6 μg/kg and from 0.5 to 47.0, respectively.
G-quartet biomolecular nanowires
A. Calzolari,R. Di Felice,E. Molinari,A. Garbesi
Physics , 2002, DOI: 10.1063/1.1476700
Abstract: We present a first-principle investigation of quadruple helix nanowires, consisting of stacked planar hydrogen-bonded guanine tetramers. Our results show that long wires form and are stable in potassium-rich conditions. We present their electronic bandstructure and discuss the interpretation in terms of effective wide-bandgap semiconductors. The microscopic structural and electronic properties of the guanine quadruple helices make them suitable candidates for molecular nanoelectronics.
Self-assembled guanine ribbons as wide-bandgap semiconductors
A. Calzolari,R. Di Felice,E. Molinari,a. Garbesi
Physics , 2001, DOI: 10.1016/S1386-9477(02)00343-0
Abstract: We present a first principle study about the stability and the electronic properties of a new biomolecular solid-state material, obtained by the self-assembling of guanine (G) molecules. We consider hydrogen-bonded planar ribbons in isolated and stacked configurations. These aggregates present electronic properties similar to inorganic wide-bandgap semiconductors. The formation of Bloch-type orbitals is observed along the stacking direction, while it is negligible in the ribbon plane. Global band-like conduction may be affected by a dipole-field which spontaneously arises along the ribbon axis. Our results indicate that G-ribbon assemblies are promising materials for biomolecular nanodevices, consistently with recent experimental results.
Ab-initio study of model guanine assemblies: The role of pi-pi coupling and band transport
R. Di Felice,A. Calzolari,E. Molinari,A. Garbesi
Physics , 2001, DOI: 10.1103/PhysRevB.65.045104
Abstract: Several assemblies of guanine molecules are investigated by means of first-principle calculations. Such structures include stacked and hydrogen-bonded dimers, as well as vertical columns and planar ribbons, respectively, obtained by periodically replicating the dimers. Our results are in good agreement with experimental data for isolated molecules, isolated dimers, and periodic ribbons. For stacked dimers and columns, the stability is affected by the relative charge distribution of the pi orbitals in adjacent guanine molecules. pi-pi coupling in some stacked columns induces dispersive energy bands, while no dispersion is identified in the planar ribbons along the connections of hydrogen bonds. The implications for different materials comprised of guanine aggregates are discussed. The bandstructure of dispersive configurations may justify a contribution of band transport (Bloch type) in the conduction mechanism of deoxyguanosine fibres, while in DNA-like configurations band transport should be negligible.
Intelligence, school, marks and pupils: A rejoinder to Raety and Snellman's (1995) 'On the social fabric of intelligence'
Carugati, Felice
Papers on Social Representations , 1996,
Beyond the intercultural: the challenges of co-inclusion The intercultural: enthusiasm, litotes and euphemism
Felice Dassetto
Revista CIDOB d'Afers Internacionals , 2004,
Abstract: In the context of new plural, multicultural societies, Dassetto aims to demonstrate that the term intercultural is a kind of euphemism used to cautiously express the uneasiness caused by the new postures related to the current processes of cultural encounter in Europe.These postures make reference, on the one hand, to the form of intercultural processes and, on the other hand, to the questions that are posed in the face of new social realities, like that of young adolescents, who work with different cultural and identity codes and,above all, who are unadapted to contemporary societies. To this, one must add an encounter with Islam, which goes beyond the simple cultural encounter and involves deeper layers of the collective organisation, that is, civilisations. Thus, Dassetto aims to make the terms that underlie “interculturality” in Europe explicit with relation to immigrant populations, and to question, in short, the representations relating to interculturality.
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