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Search Results: 1 - 10 of 2433 matches for " Quevedo-Hidalgo Balkys "
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Inmovilización de hongos ligninolíticos para la remoción del colorante negro reactivo 5
Fernández,Jorge Andrés; Henao,Lina María; Pedroza-Rodríguez,Aura Marina; Quevedo-Hidalgo,Balkys;
Revista Colombiana de Biotecnología , 2009,
Abstract: waste water from the textile industry represents a major environmental and health problem because it contains azo dyes whose carcirogenic effect has been tested in research. biological treatment represents a valuable alternative for removing these dyes. the effect of trametes versicolor, pleurotus ostreatus and phanerochaete chrysosporium rot fungi on decoloration of water containing reactive black five (nr5) textile dye was evaluated in this work. immobilising the fungi on polyurethane foam and luffa sponge (luffa cylindrica) supports was studied in order to select the best support and the fungi having the best decolorisation. both supports were equally effective; however, the luffa sponge was selected as being a natural product. trametes versicolor produced the highest decolorisation percentages in four days (96%, 98% and 98% for 300 ppm, 150 ppm and 75 ppm nr5 concentrations, respectively) while lacase enzyme activity was 8 ul-1, 7 ul-1 and 5 ul-1 for each of them.
Evaluación de la capacidad probiótica "in vitro" de una cepa nativa de Saccharomyces cerevisiae
Ortiz,ángela; Reuto,Joanna; Fajardo,Erika; Sarmiento,Sandra; Aguirre,Andrea; Arbeláez,Gustavo; Gómez,David; Quevedo-Hidalgo,Balkys;
Universitas Scientiarum , 2008,
Abstract: the in vitro probiotic capacity of a native strain of saccharomyces cerevisiae (a) was evaluated and compared with a commercial strain (b) used as a probiotic. the effect of the concentration of sugarcane molasses (10, 20 and 30% (w/v)) on the biomass production was investigated and kinetic parameters were determined. the best molasses concentration was 20% (w/v) and differences in biomass production on molasses medium between strain a (28 g/l) and control strain b (3 g/l) were observed. in vitro tests such as tolerance to bile salts, ph and gastric juices were carried out, and no differences in growth between strain a and b were found. cholesterol reduction on presence of bile salts after 12 hours of incubation was of 54% for strain a and 58% for strain b. both strains showed adherence to caco-2 cells. results reveal that strain a possesses in vitro probiotic properties that can be verified with further in vivo studies to confirm its suitability as probiotic in animal nutrition.
Inmovilización de hongos ligninolíticos para la remoción del colorante negro reactivo 5
Fernández Jorge Andrés,Henao Lina María,Pedroza-Rodríguez Aura Marina,Quevedo-Hidalgo Balkys
Revista Colombiana de Biotecnología , 2009,
Abstract: Immobilising lignilolytic fungus for removing reactive black 5 dye Resumen: La presencia de colorantes azoicos en aguas residuales de la industria textil es un problema ambiental y sanitario, porque muchos de estos compuestos son cancerígenos. Los tratamientos biológicos son una alternativa para la remoción de ese tipo de colorantes. En el presente trabajo se evaluó el efecto de tres hongos de podredumbre blanca, Trametes versicolor, Pleurotus ostreatus y Phanerochaete chrysosporium sobre la decoloración de un agua que contiene colorante negro reactivo 5 (NR5), ampliamente usado en la industria textil. Se estudió la inmovilización de estos hongos en dos soportes, espuma de poliuretano y estropajo (L. cylíndrica) para seleccionar el mejor soporte y el hongo con mayor capacidad para la decoloración. Ambos soportes fueron igualmente efectivos, pero se seleccionó estropajo por ser un producto natural. El hongo que generó los mayores porcentajes de decoloración en 4 días fue Trametes versicolor, con 96%, 98% y 98% para agua con concentración de NR5 300 ppm, 150 ppm y 75 ppm, respectivamente. La actividad lacasa para cada concentración de NR5 fue 8 U L-1, 7 U L-1 y 5 U L-1. Palabras clave: , Trametes versicolor; Pleurotus ostreatus y Phanerochaete chrysosporium Luffa cylindrinca; espuma de poliuretano y negro reactivo 5. Abstract: Waste water from the textile industry represents a major environmental and health problem because it contains azo dyes whose carcirogenic effect has been tested in research. Biological treatment represents a valuable alternative for removing these dyes. The effect of Trametes versicolor, Pleurotus ostreatus and Phanerochaete chrysosporium rot fungi on decoloration of water containing reactive black five (NR5) textile dye was evaluated in this work. Immobilising the fungi on polyurethane foam and luffa sponge (Luffa cylindrica) supports was studied in order to select the best support and the fungi having the best decolorisation. Both supports were equally effective; however, the luffa sponge was selected as being a natural product. Trametes versicolor produced the highest decolorisation percentages in four days (96%, 98% and 98% for 300 ppm, 150 ppm and 75 ppm NR5 concentrations, respectively) while lacase enzyme activity was 8 UL-1, 7 UL-1 and 5 UL-1 for each of them. Key words: Trametes versicolor; Pleurotus ostreatus; Phanerochaete chrysosporium Luffa cylindrinca; polyurethane foam; reactive black 5 dye.
Evaluación de actividades enzimáticas de Fusarium spp., aislados de lesiones en humanos, animales y plantas
María Fernanda Valencia-Guerrero,Balkys Quevedo-Hidalgo,Marcela Franco-Correa,Hugo Díez-Ortega
Universitas Scientiarum , 2011,
Abstract: Assessment of enzymatic characterization of Fusarium spp. isolated from human, animal, and plant wounds. Objective. Todetermine amylolytic, cellulolytic, lipolytic, pectinolytic and proteolytic activities in 32 Fusarium spp. isolates from humans, animals andplants. Materials and methods. Qualitative determination of enzymatic activities was done by measuring hydrolysis halos in agar plateswith their corresponding substrate. Quantitative determination was done by colorimetric techniques, using liquid culture supernatantsto determine the respective substrate degradation. Results. All isolates showed enzymatic activities from a qualitative point of view,except amylolytic and lipolytic. Quantitative determination was possible for all the evaluated enzymes except lipases. Conclusion.The determination of amylolytic, cellulolytic, pectinolytic and proteolytic enzymatic profiles of each of the Fusarium isolates assessed suggests their capacity to degrade these substrates, irrespectively of their origin.
Escuela de la muerte. Una mirada desde la antropología forense
Quevedo-Hidalgo,Helka Alejandra;
Universitas Humanística , 2008,
Abstract: through exercising anthropology in a legal context, i was able to observe the existence and consequences of cruel and violent acts carried out by non-governmental armed groups - in this case paramilitary - which due to their structure, methodology and organization allow me to draw a comparison of these acts with what i have called a "school of death" with the functionality of an academic school - for life. i reflect about the position of the victim, the aggressor and mine as a forensic expert and a person that is interfering with this school of death. the former, based on the description and study of a forensic case that represents one of several judicial diligences carried out by state forensic teams before law 975 of 2005 took effect, known as the "law of justice and peace", which, according to the legal text, "has as an objective to facilitate the peace processes and the individual or collective reincorporation into civil life of members of armed groups at the margins of the law, guaranteeing the right of the victims for truth, justice and reparation."
Escuela de la muerte. Una mirada desde la antropología forense
Helka Alejandra Quevedo-Hidalgo
Universitas Humanística , 2008,
Abstract: Through exercising anthropology in a legal context, I was able to observe the existence and consequences of cruel and violent acts carried out by non-governmental armed groups – in this case paramilitary – which due to their structure, methodology and organization allow me to draw a comparison of these acts with what I have called a “school of death” with the functionality of an academic school – for life. I reflect about the position of the victim, the aggressor and mine as a forensic expert and a person that is interfering with this School of Death. The former, based on the description and study of a forensic case that represents one of several judicial diligences carried out by state forensic teams before Law 975 of 2005 took effect, known as the “Law of Justice and Peace”, which, according to the legal text, “has as an objective to facilitate the peace processes and the individual or collective reincorporation into civil life of members of armed groups at the margins of the law, guaranteeing the right of the victims for truth, justice and reparation.”
Inmovilización de Bacillus licheniformis y Saccharomyces cerevisiae para la producción de etanol a partir de almidón de papa
Sossa-Urrego,Diana; Navarro-Acevedo,María Angélica; Matiz Villamil,Adriana; Mercado-Reyes,Marcela; Quevedo-Hidalgo,Balkys; Pedroza-Rodríguez,Aura Marina;
Universitas Scientiarum , 2008,
Abstract: we evaluated a sequential discontinuous system composed by bacillus licheniformis and saccharomyces cerevisiae for ethanol production. for the second phase of the process potato starch hydrolyzed were used, which was obtained from b. licheniformis cells. both microorganisms were immobilized in a calcium alginate matrix of 3,2% and 2,5% (w/v), where was observed that these concentrations retained the majority of the cells (26x106 and 10x107 ufc/g) and allowed dissemination of its products, gaining 3.3 g/l of reducing sugars and 642 au/l (units amylolytic) for b. licheniformis and 0,866% (v/v) ethanol with s. cerevisiae. by means of a 22 factorial design were selected operating conditions at a reactor scale for production of hydrolyzed, finding that by cultivating b. licheniformis with 3 v.v.m. and 150 r.p.m. there were 3.7 g/l of reducing sugars and 669 au/l after 4 hours of the process. the hydrolyzed was characterized using hplc chromatography, which determined that it is rich in oligomers and dextrin, and it has low concentration of glucose and maltose. the use of hydrolyzed for ethanol production, generated low percentages (0,47% and 0,74% v/v) in free and immobilized cells respectively.
Evaluación de actividades enzimáticas de Fusarium spp., aislados de lesiones en humanos, animales y plantas
Valencia-Guerrero,María Fernanda; Quevedo-Hidalgo,Balkys; Franco-Corrrea,Marcela; Díez-Ortega,Hugo; Parra-Giraldo,Claudia Marcela; Rodríguez-Bocanegra,María Ximena;
Universitas Scientiarum , 2011,
Abstract: objective. to determine amylolytic, cellulolytic, lipolytic, pectinolytic and proteolytic activities in 32 fusarium spp. isolates from humans, animals and plants. materials and methods. qualitative determination of enzymatic activities was done by measuring hydrolysis halos in agar plates with their corresponding substrate. quantitative determination was done by colorimetric techniques, using liquid culture supernatants to determine the respective substrate degradation. results. all isolates showed enzymatic activities from a qualitative point of view, except amylolytic and lipolytic. quantitative determination was possible for all the evaluated enzymes except lipases. conclusion. the determination of amylolytic, cellulolytic, pectinolytic and proteolytic enzymatic profiles of each of the fusarium isolates assessed suggests their capacity to degrade these substrates, irrespectively of their origin.
Efecto del sustrato y la exposición a la luz en la producción de una cepa de Trichoderma sp.
Chávez-García,Mónica; Monta?a-Lara,José Salvador; Martínez-Salgado,María Mercedes; Mercado-Reyes,Marcela; Rodríguez,María Ximena; Quevedo-Hidalgo,Balkys;
Universitas Scientiarum , 2008,
Abstract: in order to evaluate the effect of temperature and light conditions on biomass production of a trichoderma sp. strain, three culture media were tested: rice 53% (w/w), rice 53% (w/w) -molasses 3% (w/w) and rice 53% (w/w)-molasses 10% (w/w) in distilled water. incubation conditions were: 25°c, constant light and a photoperiod of 24 h light/24 h darkness during 8 days. the evaluated parameters were population density (conidia/ml), spore germination after 24 hours and purity percentage. the results showed that solid fermentation using rice - distilled water as substrate at 25°c and constant light, allowed the highest conidia yield (45x1018 conidia/ml), 96% germination after 24 hours, and 92.1% purity. the liquid fermentation rendered a purity of 76.8% and conidia germination of 91.2% after 24 hours, showing a disadvantageous lower purity percentage compared to solid fermentation.
Evaluación de tres hongos lignolíticos y de Aspergillus niger como alternativa para el tratamiento de aguas residuales del curtido de pieles
GóMEZ-BERTEL, Sandra;AMAYA-BULLA, Diana;MALDONADO-SAAVEDRA, Claudia;MARTíNEZ-SALGADO, María Mercedes;QUEVEDO-HIDALGO, Balkys;SOTO-GUZMáN, Ana Bertha;PEDROZA-RODRíGUEZ, Aura Marina;
Revista internacional de contaminación ambiental , 2008,
Abstract: in order to evaluate the capacity of chromium and color removal of the white rot fungus pleurotus ostreatus, phanerochaete chrysosporium, trametes versicolor and the deuteromycete aspergillus niger, using chromium salts and tanning wastewater, the tolerance to two chromium salts using the inhibitory minimum concentration test (imc) was studied. according to the results, significant differences (p <0.0001) demonstrate that p. chrysosporium was the most tolerant microorganism with imc of 30,000 mg l-1 for chromium sulfate and 7,500 mg l-1 for dichromate of potassium. the effect of the dilution of residual on the capacity of biodecoloration and the formation of biomass were evaluated using a 22 experimental design. p. chrysosporium displayed greater capacity of decoloration associated with the amount of biomass with values of 39% and 16 mg l-1 in 10 days of evaluation. when supplementing this microorganism with carbon and nitrogen sources it was observed that both nutritional conditions could be used without significant differences (p>0.0001) between treatments. from these results the microorganisms were immobilized in polyurethane foam to carry out the experiments of treatment capacity of residual to the 100% by 10 days at 25° c, observing that the immobilized viable biomass removed 97% of crt, 97% of cr(iii), 85% cr(vi), 12% dqo and 67% of color. these parameters were correlated positively with the amount of biomass immobilized and the enzymatic activity type lip (0.2 u l-1) and mnp (7 u l-1). the immobilized and inactivated biomasses, and the foam as unique support, were also efficient concerning the previous parameters but in smaller proportion. micro edx analysis indicated that both the viable and inactive biomasses present important adsorptive characteristics that favored the expressed total chromium removal in atomic percentage.
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