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Search Results: 1 - 10 of 49563 matches for " QIAO Jian-fang "
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THE ULTRASTRUCTURE OF EGGSHELL OF Otis undalata macqueenii AND Otis tarda tarda

QIAO Jian-Fang,GAO Xing-Yi,YANG Wei-Kang,

动物学研究 , 2000,
Abstract: 利用扫描电镜对波斑鸨(Otis undulata macqueenii)和大鸨(Otis tarda tarda)卵壳的壳膜层、锥体层、海绵层和表层的超微结构进行了比较研究。结果显示,作为同属鸟类,它们的外壳表面具有相似性,即均具有突起及大小不等的气孔,壳膜纤维交错成网状、海绵层分布有较多蜂窝小孔等。但作为不同的种,二者又具有显著差异,这种结构上的差异与种的特异性、成鸟的本重、卵的大小及生存环境密
Diurnal behaviors time budgets and activity rhythms of Gazella subgutturosa in winter.

XIA Can-jun,QIAO Jian-fang,YANG Wei-kang,XU Wen-xuan,LIU Wei,LI Ying,

生态学杂志 , 2009,
Abstract: 2007年12月,采用目标动物取样法在卡拉麦里山有蹄类自然保护区观察鹅喉羚冬季昼间行为。将鹅喉羚的行为分为采食、警戒、休息、移动和“其他”5种类型,各类行为所占比例雌羊为68.0%、7.0%、19.6%、5.0%、0.4%,采食行为消耗的时间最多,“其他”行为消耗时间最短,雄羊为29.6%、19.2%、29.3%、20.6%、1.3%,采食行为所消耗时间仍占最多,但较雌羊已大幅减少。无论雌雄,各行为在10 min观察期内所占据平均时间均存在极显著差异(P<0.01);除“其他”行为在10 min观察期内所占据平均时间性别间差异不显著外,雌雄其余4类行为间差异均极显著(P<0.01)。雌羊采食行为存在3个高峰,分别在11:00—12:00、13:00—14:00和17:00—18:00;移动高峰出现在13:00—15:00和18:00—19:00;警戒最高峰出现在13:00—14:00;休息行为表现为双峰形,分别在12:00—13:00和16:00—17:00。雄羊采食行为也有3个高峰,分别在10:00—11:00、13:00—14:00和17:00—18:00,但不如雌性明显;移动行为在13:00—14:00和18:00—19:00有2个高峰;警戒行为在13:00—14:00和18:00—19:00出现小的高峰;休息在15:00—16:00达到最高峰。雌性鹅喉羚采食、移动、警戒、休息行为在各个时段差异均显著,而雄性则是采食和警戒存在显著差异。
Cooperation of Mtmr8 with PI3K Regulates Actin Filament Modeling and Muscle Development in Zebrafish
Jie Mei, Zhi Li, Jian-Fang Gui
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0004979
Abstract: Background It has been shown that mutations in at least four myotubularin family genes (MTM1, MTMR1, 2 and 13) are causative for human neuromuscular disorders. However, the pathway and regulative mechanism remain unknown. Methodology/Principal Findings Here, we reported a new role for Mtmr8 in neuromuscular development of zebrafish. Firstly, we cloned and characterized zebrafish Mtmr8, and revealed the expression pattern predominantly in the eye field and somites during early somitogenesis. Using morpholino knockdown, then, we observed that loss-of-function of Mtmr8 led to defects in somitogenesis. Subsequently, the possible underlying mechanism and signal pathway were examined. We first checked the Akt phosphorylation, and observed an increase of Akt phosphorylation in the morphant embryos. Furthermore, we studied the PH/G domain function within Mtmr8. Although the PH/G domain deletion by itself did not result in embryonic defect, addition of PI3K inhibitor LY294002 did give a defective phenotype in the PH/G deletion morphants, indicating that the PH/G domain was essential for Mtmr8's function. Moreover, we investigated the cooperation of Mtmr8 with PI3K in actin filament modeling and muscle development, and found that both Mtmr8-MO1 and Mtmr8-MO2+LY294002 led to the disorganization of the actin cytoskeleton. In addition, we revealed a possible participation of Mtmr8 in the Hedgehog pathway, and cell transplantation experiments showed that Mtmr8 worked in a non-cell autonomous manner in actin modeling. Conclusion/Significance The above data indicate that a conserved functional cooperation of Mtmr8 with PI3K regulates actin filament modeling and muscle development in zebrafish, and reveal a possible participation of Mtmr8 in the Hedgehog pathway. Therefore, this work provides a new clue to study the physiological function of MTM family members.
Fish-Specific Duplicated dmrt2b Contributes to a Divergent Function through Hedgehog Pathway and Maintains Left-Right Asymmetry Establishment Function
Sha Liu, Zhi Li, Jian-Fang Gui
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0007261
Abstract: Gene duplication is thought to provide raw material for functional divergence and innovation. Fish-specific dmrt2b has been identified as a duplicated gene of the dmrt2a/terra in fish genomes, but its function has remained unclear. Here we reveal that Dmrt2b knockdown zebrafish embryos display a downward tail curvature and have U-shaped somites. Then, we demonstrate that Dmrt2b contributes to a divergent function in somitogenesis through Hedgehog pathway, because Dmrt2b knockdown reduces target gene expression of Hedgehog signaling, and also impairs slow muscle development and neural tube patterning through Hedgehog signaling. Moreover, the Dmrt2b morphants display defects in heart and visceral organ asymmetry, and, some lateral-plate mesoderm (LPM) markers expressed in left side are randomized. Together, these data indicate that fish-specific duplicated dmrt2b contributes to a divergent function in somitogenesis through Hedgehog pathway and maintains the common function for left-right asymmetry establishment.
An Apo-14 Promoter-Driven Transgenic Zebrafish That Marks Liver Organogenesis
Rui Wang,Zhi Li,Yang Wang,Jian-Fang Gui
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0022555
Abstract: Several transgenic zebrafish lines for liver development studies had been obtained in the first decade of this century, but not any transgenic GFP zebrafish lines that mark the through liver development and organogenesis were reported. In this study, we analyzed expression pattern of endogenous Apo-14 in zebrafish embryogenesis by whole-mount in situ hybridization, and revealed its expression in liver primordium and in the following liver development. Subsequently, we isolated zebrafish Apo-14 promoter of 1763 bp 5′-flanking sequence, and developed an Apo-14 promoter-driven transgenic zebrafish Tg(Apo14: GFP). And, maternal expression and post-fertilization translocation of Apo-14 promoter-driven GFP were observed in the transgenic zebrafish line. Moreover, we traced onset expression of Apo-14 promoter-driven GFP and developmental behavior of the expressed cells in early heterozygous embryos by out-crossing the Tg(Apo14: GFP) male to the wild type female. Significantly, the Apo-14 promoter-driven GFP is initially expressed around YSL beneath the embryo body at 10 hpf when the embryos develop to tail bud prominence. In about 14-somite embryos at 16–17 hpf, a typical “salt-and-pepper” expression pattern is clearly observed in YSL around the yolk sac. Then, a green fluorescence dot begins to appear between the notochord and the yolk sac adjacent to otic vesicle at about 20 hpf, which is later demonstrated to be liver primordium that gives rise to liver. Furthermore, we investigated dynamic progression of liver organogenesis in the Tg(Apo14: GFP) zebrafish, because the Apo-14 promoter-driven GFP is sustainably expressed from hepatoblasts and liver progenitor cells in liver primordium to hepatocytes in the larval and adult liver. Additionally, we observed similar morphology between the liver progenitor cells and the GFP-positive nuclei on the YSL, suggesting that they might originate from the same progenitor cells in early embryos. Overall, the current study provides a transgenic zebrafish line that marks the through liver organogenesis.
Mtmr8 is essential for vasculature development in zebrafish embryos
Jie Mei, Sha Liu, Zhi Li, Jian-Fang Gui
BMC Developmental Biology , 2010, DOI: 10.1186/1471-213x-10-96
Abstract: During early stage of somitogenesis, mtmr8 expression was detected in both somitic mesodem and ventral mesoderm. Knockdown of mtmr8 by morpholino impairs arterial endothelial marker expression, and results in endothelial cell reduction and vasculogenesis defects, such as retardation in intersegmental vessel development and interruption of trunk dorsal aorta. Moreover, mtmr8 morphants show loss of arterial endothelial cell identity in dorsal aorta, which is effectively rescued by low concentration of PI3K inhibitor, and by over-expression of dnPKA mRNA or vegf mRNA. Interestingly, mtmr8 expression is up-regulated when zebrafish embryos are treated with specific inhibitor of Hedgehog pathway that abolishes arterial marker expression.These data indicate that Mtmr8 is essential for vasculature development in zebrafish embryos, and may play a role in arterial specification through repressing PI3K activity. It is suggested that Mtmr8 should represent a novel element of the Hedgehog/PI3K/VEGF signaling cascade that controls arterial specification.MTM (myotubularin myopathy) family factors are members of the growing class of dual-specificity phosphatases (DSPs) including PTEN, which can dephosphorylate the products of phosphoinositide 3-kinases (PI3K), and are negative regulators of the PI3K/Akt signaling pathways[1]. A potential function for PI3K and PTEN has been suggested in both angiogenic signaling[2,3] and various models of muscle defects[4]. And, dual-specific phosphatase-5 (Dusp-5) has been identified to play a functional role in vascular development through counteracting the function of Snrk-1, a serine threonine kinase in angioblast development[5]. However, functions of MTM family members in these processes are not clear.In vertebrates, vascular and muscular systems are tightly connected. And, the cells in dorsal aorta and myotomal muscle are both derived from mesoderm. Endothelial cells (ECs) forming intersegmental vessel (SE) migrate following a path initially a
A Desolate Soul Lives a Dignified Life: The Theme of Hemingway''''s A Clean, Well-Lighted Place
孤寂的心灵 自尊的生活——论海明威《一个干净明亮的地方》的主题

MAO Jian-fang,

浙江大学学报(人文社会科学版) , 2000,
Abstract: 本文通过分析探讨美国作家海明威的短篇小说《一个干净明亮的地方》的主题来揭示作者深刻的思想意蕴。海明威把孤独和空虚视为现代生活的主要悲剧,认为面对这个充满“虚无缥缈”的社会,人类需要光亮和秩序来重新确立生活的自尊,这种自尊就是生活的勇气,人类用它来战胜那可怕的精神危机。
[μ-(4S,5S,15S,16S)-10,21-Di-tert-butyl-4,5,15,16-tetraphenyl-3,6,14,17-tetraazatricyclo[,12]tetracosa-1(23),8,10,12(24),19,21-hexaene-23,24-diolato-κ8N3,N6,O23,O24:N14N17,O23,O24]bis[(acetato-κO)zinc(II)] ethanol disolvate
Li-Jing Fan,Jian-Fang Ma,Jie Liu
Acta Crystallographica Section E , 2009, DOI: 10.1107/s1600536809021990
Abstract: In the title compound, [Zn2(C36H42N4O2)(CH3COO)2]·2CH3CH2OH, a centrosymmetric dinuclear zinc macrocyclic complex is accompanied by two half-occupied ethanol solvent molecues resulting in a 1:2 macrocycle–solvent composition. The ZnII atom has a square-pyramidal geometry arising from an N2O3 donor set, being coordinated by two N atoms and two O atoms from the macrocyclic ligand in the equatorial sites and one O atom from an acetate anion in the apical site. The two ZnII atoms are linked by two phenolate O atoms, generating a four-membered Zn2O2 ring at the centre of the macrocycle. The tert-butyl group shows rotational disorder over two sets of sites in a 0.552 (12):0.448 (12) ratio. In the crystal, N—H...O and O—H...O hydrogen bonds are seen and a short intramolecular C—H...O contact occurs.
Li-Jing Fan,Jian-Fang Ma,Bo Liu
Acta Crystallographica Section E , 2009, DOI: 10.1107/s1600536809028530
Abstract: In the title centrosymmetric dinuclear zinc(II) complex, [Zn2(C36H42N4O2)(NO3)2], the ZnII atom has a distorted octahedral geometry, defined by two N atoms and two O atoms from the macrocyclic ligand and two O atoms from a chelating nitrate anion and are bridged by two phenolate O atoms, forming a four-membered Zn2O2 ring.
Hong-Ye Bai,Hua Wu,Jian-Fang Ma
Acta Crystallographica Section E , 2008, DOI: 10.1107/s1600536808033473
Abstract: The title compound {systematic name: 2,2′,2′′,2′′′-[3,7,11,15-tetraaza-1(1,4),5(1,3),9(1,4),13(1,3)-tetrabenzenacyclohexadecaphane-3,7,11,15-tetrayltetramethylene]tetrabenzonitrile}, C64H56N8, is a centrosymmetric macrocycle that is consolidated into the crystal structure by C—H...π interactions.
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