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Search Results: 1 - 10 of 6403 matches for " Putcha Venkata Lakshmana Rao "
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Characterization of Chikungunya Virus Induced Host Response in a Mouse Model of Viral Myositis
Rekha Dhanwani, Mohsin Khan, Vinay Lomash, Putcha Venkata Lakshmana Rao, Hinh Ly, Manmohan Parida
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0092813
Abstract: While a number of studies have documented the persistent presence of chikungunya virus (CHIKV) in muscle tissue with primary fibroblast as the preferable cell target, little is known regarding the alterations that take place in muscle tissue in response to CHIKV infection. Hence, in the present study a permissive mouse model of CHIKV infection was established and characterized in order to understand the pathophysiology of the disease. The two dimensional electrophoresis of muscle proteome performed for differential analysis indicated a drastic reprogramming of the proteins from various classes like stress, inflammation, cytoskeletal, energy and lipid metabolism. The roles of the affected proteins were explained in relation to virus induced myopathy which was further supported by the histopathological and behavioural experiments proving the lack of hind limb coordination and other loco-motor abnormalities in the infected mice. Also, the level of various pro-inflammatory mediators like IL-6, MCP-1, Rantes and TNF-α was significantly elevated in muscles of infected mice. Altogether this comprehensive study of characterizing CHIKV induced mouse myopathy provides many potential targets for further evaluation and biomarker study.
Evidence of Experimental Vertical Transmission of Emerging Novel ECSA Genotype of Chikungunya Virus in Aedes aegypti
Ankita Agarwal,Paban Kumar Dash ,Anil Kumar Singh,Shashi Sharma,Natarajan Gopalan,Putcha Venkata Lakshmana Rao,Man Mohan Parida,Paul Reiter
PLOS Neglected Tropical Diseases , 2014, DOI: 10.1371/journal.pntd.0002990
Abstract: Background Chikungunya virus (CHIKV) has emerged as one of the most important arboviruses of public health significance in the past decade. The virus is mainly maintained through human-mosquito-human cycle. Other routes of transmission and the mechanism of maintenance of the virus in nature are not clearly known. Vertical transmission may be a mechanism of sustaining the virus during inter-epidemic periods. Laboratory experiments were conducted to determine whether Aedes aegypti, a principal vector, is capable of vertically transmitting CHIKV or not. Methodology/Principal Findings Female Ae. aegypti were orally infected with a novel ECSA genotype of CHIKV in the 2nd gonotrophic cycle. On day 10 post infection, a non-infectious blood meal was provided to obtain another cycle of eggs. Larvae and adults developed from the eggs obtained following both infectious and non-infectious blood meal were tested for the presence of CHIKV specific RNA through real time RT-PCR. The results revealed that the larvae and adults developed from eggs derived from the infectious blood meal (2nd gonotrophic cycle) were negative for CHIKV RNA. However, the larvae and adults developed after subsequent non-infectious blood meal (3rd gonotrophic cycle) were positive with minimum filial infection rates of 28.2 (1:35.5) and 20.2 (1:49.5) respectively. Conclusion/Significance This study is the first to confirm experimental vertical transmission of emerging novel ECSA genotype of CHIKV in Ae. aegypti from India, indicating the possibilities of occurrence of this phenomenon in nature. This evidence may have important consequence for survival of CHIKV during adverse climatic conditions and inter-epidemic periods.
Built Environment Factors, Psychosocial Factors and Diabetes Mellitus: A South Indian Study
Gumpeny R Sridhar, Pasala Sudhir Kumar, Putcha Venkata, Appa Rao Allam, Vijay Kishore Durai, Madhu Kosuri, Manda R Narasinga Rao, V.K. Kumar, Zibin Jiang and Gumpeny Lakshmi
Indian Journal of Clinical Medicine , 2012, DOI: 10.4137/IJCM.S4710
Abstract: We assessed the contribution of selected built environment factors to body weight in a pilot study in urban Visakhapatnam, South India. Participants were 123 men and 60 women (age 16 to 69 years; BMI 17.3–30.5) who had lived in the area for at least 3 years. Individuals with lower BMI tended to be (a) working people (non-home based—working away from home), (b) non-vegetarians, (c) physically active (activity mostly related to work), and (d) taking afternoon siestas. Psychological stress, quality of life and wellbeing data were used from an earlier study of individuals with diabetes mellitus. The measures included were depression, anxiety, energy, positive wellbeing, satisfaction, impact, and social worry and diabetes worry (Diabetes quality of life). Guttman’s Smallest Space Analysis (SSA) suggested the relationships among the psychosocial measures can be accounted for by one facet with three axial sets of variables (a) positive wellbeing and energy, (b) satisfaction, impact, and social worry and diabetes worry, and (c) anxiety and depression. SSAs on male participants suggested that fasting blood glucose and weight were most closely associated with anxiety and energy levels. In female participants, weight and fasting glucose were most closely associated with energy and to a somewhat lesser extent with anxiety. In both sexes, age was closely associated with positive wellbeing. Also in both sexes, age, weight, and fasting glucose levels were closely associated with each other. The results support the importance of understanding the impact of built environment and psychosocial factors on body weight in diabetic individuals for designing prevention strategies.
Method Development and Validation for Simultaneous Estimation of Montelukast Sodium and Desloratadine by RP-HPLC  [PDF]
Bonthu Mohan Gandhi, Atmakuri Lakshmana Rao, Jangala Venkateswara Rao
American Journal of Analytical Chemistry (AJAC) , 2015, DOI: 10.4236/ajac.2015.68063
Abstract: A novel, precise, accurate, rapid and cost effective isocratic reverse-phase high performance liquid chromatographic (RP-HPLC) method was developed, optimized and validated for the simultaneous estimation of Montelukast Sodium (MON) and Desloratadine (DES) in pharmaceutical dosage forms. The drugs were estimated using Hypersil BDS C18 (250 mm × 4.6 mm I.D., 5 μ particle size) column. The mobile phase composed of orthophosphoric acid and water in the ratio of 20:80 v/v, at a flow rate of 1.0 ml/min was used for the separation. Detection was carried out at 280 nm. The linearity range obtained was 10 - 30 μg/ml for MON and 5 - 15 μg/ml for DES with retention times of 2.929 min and 4.439 min for MON and DES respectively. The correlation coefficient values were found to be 0.999. Precision studies showed % RSD values less than 2% for both the drugs in all the selected concentrations. The percentage recoveries of MON and DES were in the range of 99.59% - 99.82% and 99.60% - 99.80% respectively. The limit of detection (LOD) and limit of quantification (LOQ) were 0.176 μg/ml, 0.587 μg/ml for MON and 0.087 μg/ml, 0.292 μg/ml for DES respectively. The method was validated as per the International Conference on Harmonization (ICH) guidelines. The proposed validated method was successfully used for the quantitative analysis of commercially available tablet dosage forms.
Unilateral linear pansclerotic morphea affecting face and limbs
Padmavathy L,Rao Lakshmana
Indian Journal of Dermatology, Venereology and Leprology , 2005,
Abstract: Disabling pansclerotic morphea is a rare atrophying and sclerosing disorder of the subcutaneous tissue, muscle and bone. It is characterized by atrophy of the skin, subcutaneous fat, muscle and bone involving half of the face. In some patients the atrophic lesions extend to involve the ipsilateral or contralateral upper and lower limbs with radiological evidence of hemiatrophy. The patients may present with arthralgia, convulsions or cramps. We report a case of a woman with deformity of face, and left upper and lower limbs that had started as an indurated plaque on the left half of forehead at the age of 5 years and had gradually enlarged, followed by the development of atrophic changes in left eye. The case is being reported in view of its rare occurrence.
Ulcerative lupus vulgaris
Padmavathy L,Rao Lakshmana
Indian Journal of Dermatology, Venereology and Leprology , 2005,
Polarizabilities and Orientational Order Parameter in N-(p-n-Ethoxybenzylidene)- p-n-Alkoxy Anilines, 2O.Om LC Compounds  [PDF]
Putcha Srinivasa Sastry, Chintalapati Srinivas, Pokkunuri Pardhasaradhi, Venkata Gopala Krishna Murthy Pisipati
Journal of Surface Engineered Materials and Advanced Technology (JSEMAT) , 2016, DOI: 10.4236/jsemat.2016.63013
Abstract: n.m, n.Om, nO.m and nO.Om compounds play an important role in the fundamental and applied aspects. As a part of our systematic studies of the above homologous series regarding the synthesis, characterization and phase transition studies, here the authors report the polarizabilities and orientational order parameter, S in N-(p-n-ethoxybenzylidene)-p-n-alkoxy anilines, 2O.Om with m = 3 to 10 liquid crystalline compounds except with m = 5 which has been already published. The rest of the compounds exhibit the nematic LC phase except with m = 10 which shows the smectic-C phase along the nematic phase which is originally not detected by Godzwon et al. But the author’s observations with TM and differential scanning calorimeter have shown the existence on SmC phase in addition to nematic phase. Further, SmC is clarified using the histogram technique whose details are given below and are published elsewhere. The orientstonal order parameter, S is estimated using different methods and compared with the value obtained from birefringence, δn = (ne no) where no field model is used. It is observed in these compounds case as in other LC compounds Vuks isotropic model is favored compared to that of anisotropic model proposed by Neugebauer.
Effect of Copper Doping on Structural, Dielectric and DC Electrical Resistivity Properties of BaTiO3  [PDF]
Moganti Venkata Someswara Rao, Kocharlakota Venkata Ramesh, Majeti Naga Venkata Ramesh, Bonthula Srinivasa Rao
Advances in Materials Physics and Chemistry (AMPC) , 2013, DOI: 10.4236/ampc.2013.31012

The modified BaTiO3 ferroelectric materials are suitable for pyroelectric applications. This paper reports the structural, dielectric and electrical properties on copper influence in BaTiO3 when it was substituted site “A” of perovskite structure of BaTiO3. Copper has been chosen for modified BaTiO3 with different concentrations with stoichiometry Ba1-xCuxTiO3, where x = 0.01%, 0.02%, 0.03% and 0.04%. The X-ray diffraction patterns of the samples doped with different composition of CuO are found to be that the positions and intensities of the diffraction peaks are similar and no secondary phases were observed. The Curie’s temperature (Tc) for all CuO doped BaTiO3 with were found to be in the range of 120°C to 125°C. The frequency dependence of relative permittivity (εr) and dielectric loss (Tanδ) of Ba1-xCuxTiO3 samples at room temperature were reported in the range 100 KHz - 1 MHz. The temperature dependence of D.C electrical resistivity studies were reported for all samples indicating that the participation of Cu

Vertical flow of a multiphase mixture in a channel
Mehrdad Massoudi,C. Lakshmana Rao
Mathematical Problems in Engineering , 2001, DOI: 10.1155/s1024123x00001459
Abstract: The flow of a multiphase mixture consisting of a viscous fluid and solid particles between two vertical plates is studied. The theory of interacting continua or mixture theory is used. Constitutive relations for the stress tensor of the granular materials and the interaction force are presented and discussed. The flow of interest is an ideal one where we assume the flow to be steady and fully developed; the mixture is flowing between two long vertical plates. The non-linear boundary value problem is solved numerically, and the results are presented for the dimensionless velocity profiles and the volume fraction as functions of various dimensionless numbers.
Transcriptomic profile of host response in Japanese encephalitis virus infection
Nimesh Gupta, PV Lakshmana Rao
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-92
Abstract: The comparison between viral replication efficiency and disease progression confirms the active role of host response in immunopathology and disease severity. The histopathological analysis confirms the severe damage in the brain in a time dependent manner. Interestingly, the transcription profile reveals significant and differential expression of various pattern recognition receptors, chemotactic genes and the activation of inflammasome. The increased leukocyte infiltration and aggravated CNS inflammation may be the cause of disease severity.This is the first report that provides a detailed picture of the host transcriptional response in a natural route of exposure and opens up new avenues for potential therapeutic and prophylactic strategies against Japanese encephalitis virus.The host response to infection is central to the effective control and ultimate clearance of invading pathogens or removal of infected cells. Infection of host with a viral pathogen marks the onset of changes in the host cell's microenvironment. Such changes in host gene expression could be a cellular antivirus response, a virus induced response that facilitate its own replication and spread or a non-specific response that neither promotes nor prevents virus infection. This alteration of expression of many cellular genes can be identified using cDNA microarray [1].Defining the transcriptional regulation of host genes on virus infection can be used as a tool to obtain an elaborate insight into mechanisms of host-virus interactions and to unravel the molecular basis of disease pathogenesis. Viruses from several families can infect neurons in the CNS (Central Nervous System) and the study of gene expression changes in the CNS during virus infection can lead to identification of new genes whose function is essential either for the promotion or prevention of virus infection [2,3].Japanese Encephalitis is one of the most dreaded mosquito borne encephalitis virus causing acute encephalitis in human
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