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Search Results: 1 - 10 of 355780 matches for " Putative virulent genes<br>猪链球菌2型 "
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Found of Putative Virulent Genes of Streptotoccus suis Type 2 Strains
猪链球菌2型可能的毒力基因的发现

TIAN Yun~,
田云
,Frank,M,Aarestrup,陆承平

微生物学报 , 2004,
Abstract: 猪链球菌2型(SS2)感染已成为影响全世界养猪业的重要问题之一。SS2菌株可分为毒力株、弱毒力株和无毒力株,但目前尚无区分此3类菌株的快速、有效的检测方法。为了获得毒力株特异的基因序列,对毒力株HA9801及无毒力株12^#进行了抑制性差减杂交(SSH)实验,获得了5个可能的新的毒力基因片段,分别是转录调节子、氨基酸通透酶、ABC转运子及表面锚定蛋白,在国内外尚属首次报道。这一发现将有助于区分SS2型菌株的毒力类型,并为SS2毒力株检测方法的建立奠定基础。
Production and Epitope Studying of Monoclonal Antibody Against IMPDH in SS2
猪链球菌2型次黄嘌呤核苷酸脱氢酶单克隆抗体的制备及其识别表位分析

ZHOU Jun-Ming,HE Kong-Wang,ZHANG Xue-Han,NI Yan-Xiu,LU Cheng-Ping,
周俊明
,何孔旺,张雪寒,倪艳秀,陆承平

微生物学通报 , 2008,
Abstract: 用纯化的硫氧还蛋白-IMPDH融合蛋白免疫BALB/c小鼠,取其脾细胞与SP2/0骨髓瘤细胞融合,对杂交瘤细胞及时筛选,阳性孔经4次有限稀释法克隆,成功获得1A8、1F2、2D2和2D12共4株能稳定传代并分泌抗IMPDH的单克隆抗体(McAb)的杂交瘤细胞株.4株腹水型单克隆抗体间接ELISA效价分别为100x211、100x211、100x210和100x28,经Western-blot分析表明,4株单抗与硫氧还蛋白-IMPDH融合蛋白均具有特异性反应,并且通过4种IMPDH全基因分片段缺失表达的融合蛋白,分析了4株单抗所识别抗原决定簇的差异性,发现1A8、1F2,2D2识别表位的编码基因集中在IMPDH基因片段的627 bp~790 bp之间,2D12识别表位的编码基因则集中在IMPDH基因片段的411 bp~790 bp之间.猪链球菌2型中IMPDH单克隆抗体的获得及相应表位分析为研究IMPDH蛋白的生物学活性及免疫学活性奠定了基础.
Cloning and characterization of the gene encoding IMPDH of Streptococcus suis serotype 2
猪链球菌2型次黄嘌呤核苷酸脱氢酶基因的克隆与鉴定

DUAN Zhi-tao,HE Kong-wang,ZHANG Xue-han,NI Yan-xiu,LU Cheng-ping,
段志涛
,何孔旺,张雪寒,倪艳秀,陆承平

微生物学报 , 2006,
Abstract: Given the lack of effective vaccines to control Streptococcus suis infection and the lack of a rapid and reliable molecular diagnostic assay to detect its infection, S. suis serotype 2 was sequenced partly in an effort to identify important virulence factors. Two new open reading frames were found located between orf2 and mrp. One of new open reading frame (2738 - 3694) that encoded a polypeptide of 319 amino acid residues with a calculated molecular mass of 33.5kDa was identified by Western blot. GenBank database search revealed that the derived amino acid sequence shared low homology with sequences of known function from other genes. Second structure was analyzed by InterPro, PHD, DNAstar software, the deduced protein had functional domains typical of IMP dehydrogenase (IMPDH). The PCR product of the open reading frame was transformed into E. coli BL21 and the fusion protein of 48kDa was expressed. The recombinant protein was reactive with serum from pigs experimentally infected with virulent strains of S. suis type 2, suggesting that the protein is immunogenic. IMPDH activity staining confirmed that the protein has IMPDH function and can catalyze the rate-limiting reaction of GTP biosynthesis, the NAD-dependent reduction of IMP into XMP. Flow cytometry (FCM) revealed that the protein had apparent effect on HEp-2 cell cycle.
2型猪链球菌溶血素多克隆和单克隆抗体制备及鉴定
Preparation and identification of polyclonal and monoclonal antibody of Suilysin in highly virulent strains of Streptococcus suis serotype 2

孙雯,郑峰
- , 2017,
Abstract: 目的 制备并鉴定2型猪链球菌(Streptococcus suis serotype 2,S.suis 2)四川分离菌株 05ZYH33 的溶血素(Suilysin,SLY)多克隆和单克隆抗体。方法 利用重组 SLY 蛋白制备 SLY 兔多抗,优化间接 ELISA 体系检测多抗效价,Western blot 分析 SLY 多抗特异性,检测 SLY 兔多抗对人外周血杀菌作用的影响。利用杂交瘤细胞融合技术制备 SLY 单抗,筛选杂交瘤细胞株并制备小鼠腹水,间接 ELISA 和 Western blot 鉴定 SLY 单抗的效价和特异性。结果 间接 ELISA 显示 SLY 多抗效价高达1∶204 800,Western blot 显示 SLY 多抗有较高的特异性和免疫反应性,人外周血杀菌试验显示,SLY 多抗能加强人外周血对 05ZYH33 的杀伤作用。间接 ELISA 检测杂交瘤细胞 2B6 和 5F7 培养上清效价分别是1∶800和1∶3 200,单抗效价都为1∶204 800。间接 ELISA 和 Western blot 鉴定显示,单抗 2B6 和 5F7 具有很强的特异性和免疫反应性。结论 成功制备了效价高、特异性强的 S.suis 2 05ZYH33 SLY 多克隆和单克隆抗体。
浦东地区部分猪场猪链球菌2型的分离鉴定及耐药性分析
Identification and Drug Resistance Analysis of Streptococcus suis Type 2 from the Pudong Area

韩少鹏,俞向前,叶承荣,朱建国
- , 2015,
Abstract: 年03月至08月期间,对来自浦东地区患关节炎的150份病猪和死猪内脏器官及关节液进行细菌分离,对分离细菌进行革兰氏染色、PCR鉴定、药敏实验和多重PCR检测链球菌毒力因子分布(荚膜多糖cps、溶菌酶释放蛋白基因mrp、胞外因子ef、溶血素sly、毒力相关基因orf2、谷氨酸脱氢酶基因gdh、甘油醛-3-磷酸脱氢酶基因gapdh),并对链球菌2型cps基因序列进行进化树分析。结果,从分离到的120株细菌中检测到21株链球菌,分离率为17.5%,2型链球菌分离率为11.67%;链球菌的毒力型呈-/+cps2J-/+mrp-ef-sly-gdh-/+gadph -/+orf2;分离株呈多重耐药性,耐药性为:大环内酯类<硝基呋喃类、氯霉素类、多肽类、氨基糖苷类、四环素类<青霉素类、头孢菌素类、亏诺酮类<林可胺类、磺胺类;cps基因与GenBank上已公布的链球菌2型荚膜多糖基因的核苷酸同源性达94%~99%。
From Mar.to Aug.2014,sick or dead pigs affected with arthrophlogosis from the Pudong area were sampled.The 120 strains of bacteria,isolated from 150 internal organs and joint fluid,were tested by gram stain and multiple PCR methods of detecting virulence factors which include genes about mrp,ef,sly,orf2,gdh and fbps.In addition,the sequence of cps gene of Streptococcus suis type 2 were compared with those of GenBank and a susceptibility testing of isolates were taken.The results showed that the virulence type of 21 strains of Streptococcus (17.5%) and 14 strains of Streptococcus suis type 2 (11.6%) was -/+cps2J-/+mrp-ef-sly-gdh-/+gadph -/+orf2.The 21 strains of Streptococcus showed multi-drug resistance and the strength of drug resistance indicated the following results:Macrolides < Nitrofurans,Fenicols,Methylmercadone,Aminoglycosides,Tetracyclines < Penicillins,Cephalosporins,Quinolones < Lincosamide,Sulfonamides.The identification homology of capsular polysaccharide gene of Streptococcus suis type 2 was 94%~99%.
Molecular cloning and immunological characterization of enolase from Streptococcus suis 2
猪链球菌2型烯醇化酶的分子克隆与免疫学特性

SUN Wen,PAN Xiu-Zhen,WANG Chang-Jun,ZHENG Feng,TANG Jia-Qi,
孙 雯
,潘秀珍,王长军,郑 峰,唐家琪

微生物学报 , 2008,
Abstract: To understand the enolase (eno) gene and its product in Streptococcus suis serotype 2 (S. suis 2), bioinformatics was adopted to analyze the whole genome sequence of the Chinese strain 05ZYH33 of S. suis 2. A highly homologous eno gene was unveiled by the genome-wide mining. A pair of specific primers was designed for the eno, and the target DNA fragment of 1.3 kb was successfully amplified using the genomic template of 05ZYH33. Subsequently, eno gene was inserted into pMD18-T vector, and then subcloned into prokaryotic expression vector pET32a, generating a recombinant expression plasmid pET32a::eno. The resulting plasmid was confirmed by direct DNA sequencing and transformed into E. coli BL21 (DE3) competent cells. Protein expression analysis showed that a 75 kD protein can be observed in 12% SDS-PAGE, indicating that the recombinant 6His-fused ENO protein can be produced in E. coli under the induction of IPTG. Western-blot experiment demonstrated clearly it shares strong specific antigenicity. Moreover, ELISA result suggested that ENO can occur on the surface of 05ZYH33 strain. Together, our data supported that ENO can function as a novel antigen, and may play pivotal roles in the severe infection of S. suis 2.
Construction and Characterization of Pilus Backbone Gene Knock-out Mutant of Streptococcus suis Serotype 2
猪链球菌2型菌毛骨架蛋白编码基因SSU2101敲除突变株的构建及其生物学功能

QIN Yue-Hong,WANG Chang-Jun,CHEN Hong-Na,PAN Xiu-Zhen,TANG Jia-Qi,
秦跃红
,王长军,陈红娜,潘秀珍,唐家琪

微生物学通报 , 2010,
Abstract: Based on the principle of homologous recombination, we construct gene knock-out mutant of the pilus backbone gene in Streptococcus suis serotype 2 virulent strain 05ZYH33. PCR analysis, Southern hybridization and RT-PCR analysis showed that the target gene was replaced by SpcR cassette. Analysis of biological characteristics showed that there were no differences in mycelial morphology, hemolytic activity and dyeing properties between the mutant and the wild type strain 05ZYH33. Virulence assays with murine model confirmed that the mutant is significantly less virulent than the wild type strain. The results suggest pili plays an important role in the pathogenesis, which laid the foundation for the study of the mechanisms of pilus assembly and biological function in the S. suis serotype 2.
Construction and Virulence Assays of the sortase F Gene Knock-out Mutant of Streptococcus suis 2
猪链球菌2型srtF基因敲除突变株的构建及其毒力

CHEN Hong-N,WANG Chang-Jun,PAN Xiu-Zhen,TANG Jia-Qi,
陈红娜
,王长军,潘秀珍,唐家琪

微生物学通报 , 2010,
Abstract: 利用同源重组基因敲除方法构建猪链球菌2型强毒株05ZYH33 srtF同源突变体。组合PCR、交叉酶切、RT-PCR结果均显示srtF突变体构建成功。突变株与强毒株的菌落形态、生长速率以及对小鼠的致病力均无显著性差异。小鼠竞争实验结果提示, 突变株在心脏的定殖及感染能力显著减弱。成功构建的srtF突变株为进一步研究srtF 的生物学功能奠定了基础。
Capsular saliva acid of Streptococcus suis 2 influences virulence and host inflammatory responses
猪链球菌2型荚膜唾液酸对细菌毒力和宿主炎症反应的影响

Jie Shi,Dan Hu,Jing Zhu,Xianyun Zhang,Tianqing Hou,Jingjing Guo,Xiuzhen Pan,Xianfu Li,Changjun Wang,
石洁
,胡丹,朱静,张先云,侯田青,郭静静,潘秀珍,李先富,王长军

微生物学报 , 2012,
Abstract: Objective] We clarified the pathogenic influence of the absence of Streptococcus suis type 2 capsular saliva acid on BLAB/c mice.Methods] The virulence of the experimental strains were compared;the distribution of strains in vivo was determined by quantitative plating.Histopathological analysis was used to qualitatively compare the different pathogenicity of wild strain and knockout strains.ELISA was used to test the levels of cytokine in whole blood cells for the stimulation of strains.Results] The virulence of mutant strains was significantly reduced,and when the genes were restored,toxicity levels were recovered to that of the wild type strain.The distribution in blood and in the brain between wild strain and knock out strains has significant difference,and Streptococcus suis type 2 strains can cause different degrees of brain damage.During the in vitro test,the mutant strains can stimulate the whole blood cells to secrete higher levels of MCP-1 and IL-6.Conclusion] Capsular saliva acid affects bacterial virulence and host cell inflammation response.As an important virulence factor of Streptococcus suis type 2,it can damage the blood brain barrier and cause meningitis.
Molecular cloning and immunological characterization of SSU2100 gene from srtBCD pilus island of Streptococcus suis 2
2型猪链球菌srtBCD菌毛岛SSU2100基因的克隆表达与免疫学特性

ZHANG Xian-Yun,WANG Chang-Jun,SHI Jie,ZHU Jing,PAN Xiu-Zhen,
张先云
,王长军,石洁,朱静,潘秀珍

微生物学通报 , 2012,
Abstract: Objective] To investigate the immunoprotection of pilus subunit SSU2100 which was encoded by srtBCD pilus island presented in the Chinese strain 05ZYH33 of S. suis 2. Methods] The SSU2100 gene was amplified by PCR, then cloned into prokaryotic expression plasmid pET28a, and expressed the recombinant SSU2100 in E. coli BL21. The SSU2100 protein was purified by affinity chromatography and analyzed by Western blotting. The mice anti-SSU2100 serum was prepared by immunizing mice with recombinant SSU2100 protein and analyzed by ELISA. Results] The high titer of the serum were obtained, Western blot result indicated that the recombinant protein can react with mice anti-SSU2100 serum. Animal test showed that vaccinating mice with recombinant SSU2100 protein conferred a significant protection. Conclusion] SSU2100 could serve as a vaccine candidate of S. suis 2, it provides the foundation for system-atically illustrating the role that srtBCD pilus island plays in pathogenicity of S. suis 2.
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