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Search Results: 1 - 10 of 401603 matches for " Priscilla M. Mfombep "
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Enzymatic activities and kinetic properties of β-glucosidase from selected white rot fungi  [PDF]
Priscilla M. Mfombep, Zachary N. Senwo, Omoanghe S. Isikhuemhen
Advances in Biological Chemistry (ABC) , 2013, DOI: 10.4236/abc.2013.32025
Abstract:

Beta-glucosidase is among the suite of enzymes produced by white rot fungi (WRF) to biodegrade plant biomass. This study investigated the enzymatic activities and kinetic properties of β-glucosidase from seventeen WRF comprised of the following species from various geographical locations: Pleurotus ostreatus, Auricularia auricular, Polyporus squamosus, Trametes versicolor, Lentinula edodes, and Grifola frondosa. All the WRF studied showed β-glucosidase activities. Significant variations in protein and carbohydrate contents were also recorded. Beta-glucosidase activities after 30 min of incubation ranged from 6.4 μg (T. versicolor) to 225 μg (G. frondosa). The calculated kinetic constant (Km) ranged from 0.47 μM (A. auricular-1120) to 719 μM (L. edodes-

Soil maltase activity by a glucose oxidase–perioxidase system
Priscilla M. Mfombep,Zachary N. Senwo
3 Biotech , 2012, DOI: 10.1007/s13205-012-0050-z
Abstract: The enzyme maltase (glucoinvertase; glucosidosucrase; maltase-glucoamylase; α-glucopyranosidase; glucosidoinvertase; α-d-glucosidase; α-glucoside hydrolase; α-1,4-glucosidase EC 3.2.1.20), is involved in the exo-hydrolysis of 1,4-α-glucosidic linkages and certain oligosaccharides into glucose which is an important energy source for soil microbes. This enzyme originates from different sources, which include plants, seaweeds, protozoa, fungi, bacteria, vertebrates, and invertebrates. The assay of soil maltase using maltose as substrate and the released glucose determined using a glucose oxidase–peroxidase system has not been explored or investigated to the best of our knowledge. A simple assay protocol using this system is proposed to evaluate and characterize maltase activity in soils. The protocol involves the release of glucose (determined using a glucose oxidase–peroxidase colorimetric approach) when 1 g soil is treated with toluene and incubated with 5 mM maltose in 67 mM sodium acetate buffer (pH 5.0) at 37 °C for 1 h. The optimal activity using this procedure was at pH 5.0 and decreased at temperatures above 70 °C. The calculated K m values ranged from 0.8 to 6.5 mM, and are comparable to those of enzymes purified from microorganisms. The Arrhenius equation plots for the activity in the four soils were linear between 20 and 70 °C. The activation energy values ranged from 34.1 to 57.2 kJ mol 1, the temperature coefficients (Q 10) ranged from 1.5 to 1.9 (avg. = 1.7), and the coefficients of variation (CV) of the proposed assay protocol for the soils used was <6%. While we recognize the availability of established assay protocols to determine soil α-glucosidase (referred in other literature as maltase) activity based on the p-nitrophenol (artificial product) released from p-nitrophenyl-α-d-glucopyranoside (artificial substrate), our interest was to assay its activity by determining the glucose (natural product) released from maltose (natural substrate).
Integrated Soil Fertility Management: Impact of Mucuna and Tithonia Biomass on Tomato (Lycopersicon esculentum L.) Performance in Smallholder Farming Systems  [PDF]
Christopher Ngosong, Priscilla M. Mfombep, Akeme C. Njume, Aaron S. Tening
Agricultural Sciences (AS) , 2015, DOI: 10.4236/as.2015.610112
Abstract: Many views, paradigms and concepts have been advocated in recent decades on soil fertility and soil conservation across the globe in order to provide sustainable solutions to the rising food and nutrition insecurity while preserving the natural resource base. Meanwhile, food and nutrition security in Sub-Saharan Africa (SSA) is mainly achieved through smallholder farming systems that are characterized by poor and declining soil fertility, which often leads to low crop yields and low income. Hence, a field trial was established to evaluate the impact of integrated soil fertility management (ISFM) practices on tomato yield and the farm-scale income in smallholder farming systems. The ISFM trial comprised a control with no input, mineral fertilizer, and organic treatments comprising sole Mucuna and Tithonia biomasses as well as their combination (Mucuna + Tithonia). Generally, tomato performance was better with organic plant biomass amendments, with significantly higher (P < 0.001) tomato yield for Mucuna + Tithonia and sole Tithonia, followed by sole Mucuna and mineral fertilizer compared to the control. Meanwhile in comparison to the control, Mucuna + Tithonia and sole Tithonia recorded 3.5 and 3.4 t ha-1 more yield, respectively, which was about twice the additional yield for sole Mucuna and mineral fertilizer with 1.8 and 1.5 t ha-1, respectively (Tukey’s HSD, P < 0.05). Similarly, the farm-scale income increased significantly (P < 0.001) for organic plant biomass, and it was most pronounced for Mucuna + Tithonia and sole Tithonia, followed by sole Mucuna and mineral fertilizer, as compared to the control (Tukey’s HSD, P < 0.05). A positive Spearman Rank Correlation was recorded between the ISFM treatments and tomato yield or the farm-scale income (r = 0.76, P < 0.05), and between tomato yield and the farm-scale income (r = 0.99, P < 0.05). These results imply that smallholder farmers in SSA can effectively use the combination of Mucuna + Tithonia biomass materials or their sole applications as basal mulch to improve tomato production. Thus, these organic amendments could be an alternative and sustainable integrated soil fertility management strategy to boost tomato production and farm-scale income without jeopardizing the sustainability of the environment. However, this
Coping, Personality Traits and Social Support in Severe Burn Survivors  [PDF]
Priscilla M. P. Frota, Daniela S. Zanini
Psychology (PSYCH) , 2013, DOI: 10.4236/psych.2013.412154
Abstract:

Objective: The present study aimed at investigating the correlations among coping, personality traits, and social support for burn survivors. Method: Participants were 48 patients from a reference burn hospital who attended group meetings at a non-governmental organization associated to that institution that offers support to burn survivors. Data were collected from February to May, 2010. The participants answered a socio-demographic questionnaire and three instruments: Coping Strategy Indicator (CSI), Personality Markers, and Social Support Questionnaire (MOS Scale). Results: The data obtained showed a significant correlation among the personality traits neuroticism and socialization and social support, as well as between socialization and coping; however, differently than expected, no significant correlation was established between social support and coping. Conclusion: Personality traits are related to the perception of support from others; however the perception of support does not influence the way burn survivors cope with their problems. These data were discussed in order to broaden the knowledge and stimulate new studies on the area, so that, based on this information, more efficient treatment plans and interventions can be designed to fulfill both the physical and emotional needs of these survivors with more competency and perceptiveness.

CALIDAD NUTRICIONAL DE LA CENA CONSUMIDA POR ADULTOS MAYORES HOSPEDADOS EN UN HOTEL DEL MUNICIPIO DE BALNEáRIO CAMBORIú, SANTA CATARINA, BRASIL
Reis F,Márcia; Vanhoni R,Priscilla;
Revista chilena de nutrición , 2009, DOI: 10.4067/S0717-75182009000200003
Abstract: objective: to evaluate the nutritional quality of the meals consumed by 105 elderly tourists in a hotel. method: to ensure the accuracy of the data, three portions of each option were collected and weighed. the three weights were then averaged to create a standard measure. the number of portions that each tourist took for their meal were recorded and later converted to grams using the standard measure. when evaluating the nutritional composition of the selected foods, calories, macronutrients, saturated fat, cholesterol, sodium and fiber content were all taken into consideration. results: most of the tourists were females (70%) from argentina (88%) between the ages of 60 and 85 (85%). it was noted that the males consumed foods with a higher caloric content (175% of recommended amounts), total fat (235%), and saturated fat, cholesterol (151%), proteins (170%) and sodium (133%). the quantities of carbohydrates (102%) and fiber (98%) were appropriate to meet dietary needs.
CALIDAD NUTRICIONAL DE LA CENA CONSUMIDA POR ADULTOS MAYORES HOSPEDADOS EN UN HOTEL DEL MUNICIPIO DE BALNEáRIO CAMBORIú, SANTA CATARINA, BRASIL NUTRITIONAL QUALITY OF THE DINNER CONSUMED BY ELDERLY PEOPLE STAYING IN A HOTEL IN BALNEáRIO CAMBORIú, BRAZIL
Márcia Reis F,Priscilla Vanhoni R
Revista Chilena de Nutricíon , 2009,
Abstract: Objetivo: evaluar la calidad nutritional de la cena consumida por 105 turistas de la tercera edad hospedados en un hotel. Método: para la estandarización previa del peso medio de los alimentos consumidos fueron pesadas tres porciones de cada una de las opciones ofrecidas. El consumo alimentario fue verificado por la observación directa de las porciones servidas en el plato, convertidas posteriormente en gramos. En la evaluación de la composición nutritional de la comida, se consideraron los datos sobre valor energético, macronutrientes, grasa saturada, colesterol, sodio y fibra. Resultados: la mayoría de los turistas era de Argentina (88%), del sexo femenino (70%), con edades entre 60 y 84 a os (85%). Se notó un alto consumo de energía (175%), grasas totales (235%), grasas saturadas (207%), colesterol (151%), proteína (170%) y sodio (133%). Se observó un consumo mayor de grasa saturada entre el sexo masculino. La ingestión fue adecuada para carbohidratos (102%) y fibras (98%). Objective: To evaluate the nutritional quality of the meals consumed by 105 elderly tourists in a hotel. Method: To ensure the accuracy of the data, three portions of each option were collected and weighed. The three weights were then averaged to create a standard measure. The number of portions that each tourist took for their meal were recorded and later converted to grams using the standard measure. When evaluating the nutritional composition of the selected foods, calories, macronutrients, saturated fat, cholesterol, sodium and fiber content were all taken into consideration. Results: Most of the tourists were females (70%) from Argentina (88%) between the ages of 60 and 85 (85%). It was noted that the males consumed foods with a higher caloric content (175% of recommended amounts), total fat (235%), and saturated fat, cholesterol (151%), proteins (170%) and sodium (133%). The quantities of carbohydrates (102%) and fiber (98%) were appropriate to meet dietary needs.
ANTIFUNGAL ACTIVITY OF METHANOLIC EXTRACT OF CASSIA TORA LEAVES AGAINST CANDIDA ALBICANS
Pawar Harshal Ashok,D’mello Priscilla M
International Journal of Research in Ayurveda and Pharmacy , 2011,
Abstract: Fungal infections remain a significant cause of morbidity and mortality despite advances in medicine and the emergence of new antifungal agents. Treatment of these infections is a real challenge to health professionals. The available antifungal drugs produce many adverse effects, show recurrence or lead to development of resistance. To overcome these problems, the development of newer antifungal compound against new targets is the matter of urgency. The present investigation was aimed at evaluation of antifungal activity of methanolic extract of Cassia tora leaves. The extract was prepared by hot continuous percolation method in Soxhlet apparatus using 90% methanol as an extracting solvent. The cup plate method was used to investigate the antifungal activity. The methanolic extract of Cassia tora leaves demonstrated significant antifungal activity against Candida albicans.The minimum inhibitory concentration was found to be 2mg/ml.Amphotericine-B was used as standard to compare the activity.
A Kuramoto coupling of quasi-cycle oscillators
Priscilla E. Greenwood,Lawrence M. Ward
Physics , 2015,
Abstract: A family of stochastic processes has quasi-cycle oscillations if the oscillations are sustained by noise. For such a family we define a Kuramoto-type coupling of both phase and amplitude processes. We find that synchronization, as measured by the phase-locking index, increases with coupling strength, and appears, for larger network sizes, to have a critical value, at which the network moves relatively abruptly from incoherence to complete synchonization as in Kuramoto couplings of fixed amplitude oscillators. We compare several aspects of the dynamics of unsynchronized and highly synchronized networks. Our motivation comes from synchronization in neural networks.
Unexpected Dramatic Response of Pretreated Invasive Thymic Malignancies on Pemetrexed-Case Report and Review of Current Treatment Modalities  [PDF]
Priscilla Raman, Veerle Surmont
Open Journal of Respiratory Diseases (OJRD) , 2012, DOI: 10.4236/ojrd.2012.24016
Abstract: Thymomas are rare and usually slowly growing tumors, originating from the epithelial layer of the thymus. Prognosis depends on the extent of invasion of adjacent tissues whereby multimodality treatment including surgery with or without adjuvant chemoradiotherapy is the preferred approach for locally advanced thymomas. For metastatic thymomas, only few chemotherapeutic options are available. We report 2 cases of patients with metastatic thymic malignancies with a dramatic response on pemetrexed treatment. The choice for this antifolate therapy is based upon a small series. Because metastatic thymic neoplasm is a rare disease, large randomised trials are not feasible. Case reports on the treatment of these malignancies are very important and can provide readers with the opportunity to deal with rare dis- eases.
Determinants of Urban Worker Earnings in Ghana: The Role of Education  [PDF]
Priscilla Twumasi Baffour
Modern Economy (ME) , 2015, DOI: 10.4236/me.2015.612117
Abstract: The paper examines the role of education in earnings determination by using all three rounds of the Urban Worker Survey of Ghana for 2004-2006 conducted by CSAE. The analysis is begun by investigating and comparing heterogeneity in earnings determinants amongst self-employed (informal sector), private sector workers and public sector workers with particular focus on education. An examination of the role education, individual and household characteristics play in facilitating entry into employment sectors is further undertaken in a multinomial logit model of occupational attainment in addition to analyzing the pattern of returns to education along the earnings distribution. After addressing biases associated with estimating earnings equations, it is observed that education plays an important role in promoting access to lucrative formal sector jobs, especially employment in the public sector but has no direct impact on earnings within the sector.
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