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OALib Journal期刊

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O Papel do Horm nio do Crescimento, da Insulina e dos Fatores de Crescimento na Fisiopatologia da Síndrome dos Ovários Policísticos
Premoli Ana Cristina Gomes
Revista Brasileira de Ginecologia e Obstetrícia , 2001,
Abstract:
Respuestas ecofisiológicas adaptativas y plásticas en ambientes secos de monta?a: Nothofagus pumilio, el árbol que acaparó los Andes australes
Premoli,Andrea C; Mathiasen,Paula;
Ecolog?-a austral , 2011,
Abstract: mountain areas impose variable selection pressures on plant species in relation with heterogeneous conditions of the physical environment along altitudinal gradients. these together with the regional climate influence widespread species inhabiting such gradients which may develop complex ecophysiological responses. nothofagus pumilio is a deciduous native forest species with the largest distributional range in patagonia. itis the dominant species of high-elevation forests where most often occurs in pure stands. at its northern range, inhabits climates of mediterranean regime, with wet winters and dry summers. we reviewed a series of previous studies on dry forests of n. pumilio at its northern range to provide a synthesis on ecophysiological responses under natural conditions in the field, common gardens, and reciprocal transplants. we evaluated the extent to which ecophysiological and functional traits of n. pumilio result from distinct selection pressures with elevation by means of plasticity or genetically-based adaptation. photosynthetic rates were higher in plants from the upper altitudinal limit under field conditions which were maintained in the common garden. this suggests a genetic control on net photosynthesis and also that no shortage for carbon assimilation exist at high elevation. therefore, photosynthetic responses and morphological traits are probably related to nitrogen economy and a shorter growing season at high elevations. in contrast, conductance and stomatal density showed plastic responses which will be advantageous for a deciduous species like n. pumilio given that the growing season coincides with drought. additionally, plants from contrasting elevations had significant differences in terms of architectural features of individuals, as well as leaf morphology and phenology under the common gardens suggesting strong genetic control. reciprocal transplants between contrasting elevations indicated that plants of low-elevation origin, which in tur
Dispersión asimétrica de semillas de Embothrium coccineum (Proteaceae) en el bosque templado de Chiloé, Chile
Rovere,Adriana E; Premoli,Andrea C;
Ecolog?-a austral , 2005,
Abstract: spatial patterns of seed dispersal are an important determinant of the spatial structure of plant populations, and their study may allow to infer the advance of a given species throughout the landscape. this is particularly relevant in a pioneer tree species like embothrium coccineum (proteaceae) which grows in disturbed environments of the temperate forest in southern south america. we evaluated spatial patterns of anemochorous seed dispersal of e. coccineum at a fragmented forest site located in northern chiloé island, chile. seed traps were placed around three isolated trees in an open forest area following the four cardinal directions. results showed that the density of dispersed seeds declined with increasing distance to the tree, thus fitting a leptokurtic dispersal distribution. about a 95% of the seeds were delivered within a 5 m radius around the mother tree, while the longest detected distance of primary dispersal was 20 m. seed dispersal from a parent plant reached different distances at each cardinal point, which resulted in an asymmetrical spatial pattern of seed distribution produced by the prevailing winds in the study area.
Detección de la actividad de la telomerasa en lesiones cervicales, mediante el Protocolo de Amplificación de Repeticiones Teloméricas (PART) no radioactivo
Pinto-Tang,Joanna; Castro,Thaís; Premoli,Gloria;
Investigación Clínica , 2005,
Abstract: worldwide, the cervical cancer is recognized as the second most common type or malignancy in women. the implementation of the papanicolaou smear has contributed notably to diminish its morbi-mortality. however, this test has a sensibility of just 80%. the detection of telomerase activity (ta) has been proposed as a diagnostic and prognostic maker of a wide variety of tumors and their precursor lesions. in this study the detection of ta was made from samples of cervical exfoliates and its association with cervical lesions and oncogenic human papillomavirus (hpv) types 16 and 18 was evaluated. a total of 32 samples of cervical exfoliates were analyzed, 15 from patients with normal cervices, 9 with squamous intraepithelial lesions (sil) and 8 with cervical cancer. in this last group the ta, detected by the non-radioactive telomeric repeat amplification protocol (trap), was present in 88% of the cases and in 11% of the cases with sil. none of the 15 samples of normal cervices was positive for the detection of the telomerase. 75% of the cervical exfoliates with ta showed amplifications of hpv type 16, hpv type 18 or both. these results are consistent with studies that point out a narrow relationship between oncogenic hpv and the presence of ta in human keratinocytes and consolidate the detection potential of this enzyme in the discovery of cervical alterations, especially when the technique is applied as a complementary tool of the clinical-citologic exam.
Proteina c reactiva y su relacion con la enfermedad periodontal y aterosclerosis
Premoli,Gloria; Villarreal A,Juana; González B,Anajulia;
Acta Odontológica Venezolana , 2008,
Abstract: the cardiovascular diseases as the atherosclerosis have some relation with the periodontal disease; specially, a great interest about the implication of the inflammatory process. otherwise, the periodontal disease and the arteriosclerosis is? of high prevalence in many countries is?? one of the main problems of public health to world-wide level and constitutes one of the causes of greater morbidity and mortality. the advance in molecular biology has been able to identify different molecules in patients with periodontal diseases with the production of different alterations as in the c-reactive protein. this protein is a?? precise marker in detection of inflammatory in the infectious processes with the production of high level of the c-reactive protein, also it is involve in the?? proinflammatory effects.? in the last decade it is?? catalogued as a factor for the cardiovascular risk by the america associations of the heart. this revision is bases on different aspects the c-reactive protein as a marker of inflammatory process with? the periodontal disease and aterosclerosis.
A system for the detection and typing of Human Papillomavirus of the lower genital tract: In situ hybridization screening and polymerase chain reaction confirmation
Premoli,Gloria; Galindo-Castro,Ivan; Ramírez,José Luis;
Investigación Clínica , 2005,
Abstract: we developed a simplified and non-isotopic in situ hybridization procedure for the detection of human papillomavirus (hpv) in routine papanicolaou cervical smears. the assay involves one oligonucleotide (malignant probe) which recognizes high risk hpv 16 and 18, and another which detects hpv 6 and 11 (benign probe). we adapted the system to fulfill the requirements of gynecologists and cytologists, assimilating their protocols and simplifying the in situ hybridization assay. when we compared the detection levels reached by the in situ hybridization versus a ladder pcr assay in 156 clinical samples, original designed for this work, the kappa coefficient between both methods is 0.945, indicating a strong agreement between the ish and the pcr assays
Evidencia morfológica e isoenzimática de hibridación natural entre Nothofagus antarctica y N. pumilio en el noroeste patagónico
QUIROGA,PAULA; VIDAL RUSSELL,ROMINA; PREMOLI,ANDREA C;
Bosque (Valdivia) , 2005, DOI: 10.4067/S0717-92002005000200004
Abstract: we studied the possible hybridization between n. pumilio and n. antarctica by analyzing morphological and isozyme characteristics of a high altitude population at laguna negra in northwestern patagonia, argentina. samples collected from this population for morphological and genetic analyses were compared to pure populations of n. pumilio and n. antarctica. pure species were statistically different from each other in leaf characteristics. a multivariate analysis indicated greater similarity between the hybrids and n. antarctica. polymorphism at laguna negra for 13 loci was 61% which was higher than that for n. antarctica and n. pumilio (58 and 43%, respectively). we found diagnostic alleles of each pure species and the hybrids resulted similar to n. antarctica. these results indicate that natural hybridization between n. antarctica and n. pumilio, and introgression into n. antarctica take place in laguna negra.
Complex bioclimatic and soil gradients shape leaf trait variation in Embothrium coccineum (Proteaceae) among austral forests in Patagonia
SOUTO,CINTIA P; PREMOLI,ANDREA C; REICH,PETER B;
Revista chilena de historia natural , 2009, DOI: 10.4067/S0716-078X2009000200004
Abstract: patterns of trait variation may be adaptive when vary in relation to an environmental gradient. in particular, leaf traits can affect productivity and competitive ability. we identify patterns of leaf size and shape variation with environmental heterogeneity in one of the most widespread tree species within temperate south america: embothrium coccineum (proteaceae). we collected leaf specimens and composite soil samples from 35 populations between 38° and 55° s latitude in patagonia, covering a wide range of mean annual precipitation (map) and mean annual temperature (mat). at each location, we measured nine morphological traits, some of which were cross correlated hence we focus on a smaller number of representative traits. we hypothesized that leaf area (la), dry mass (dm), and specific leaf area (sla) would increase, and that leaf shape (sf) would be more elongated, with increasing temperature, precipitation, and soil nutrient availability. we also expected growing season climate to be more closely associated with leaf traits than mean annual metrics. we used bivariate and backward stepwise multiple regressions to analyse the dependence of morphological traits with climatic and edaphic metrics. la and dm increased with increasing summer rainfall or winter temperature, as hypothesized. opposite to our hypothesis, la and dm decreased with increasing summer temperature suggesting that in terms of leaf size, e. coccineum may sense summer conditions largely as an increasing aridity stressful gradient. surprisingly, sla increased with increasingly warm or dry summers. sf was related positively to mat and negatively to map, suggesting that under more benign western climate regimes e. coccineum leaves tend to be elongated. across sites, la and dm increased with soil organic carbon and available phosphorus, and decreased with soil nitrogen and exchangeable cations. the opposite pattern was observed for sla. biologically meaningful climate metrics and soil nutrient conditi
Evaluación del Hidróxido de Calcio en la Prevención del Dolor Endodóntico Intercitas en Pulpas Necróticas
Máiquez,Silvia; Premoli de Percoco,Gloria; González,Anajulia;
Revista Cubana de Estomatolog?-a , 2001,
Abstract: postoperative pain as a result of the canalicular therapy is a very frequent side effect that may last a few hours to several days in the endodontic treatment due to the inflammatory reaction in the periradicular area. since the efficacy of calcium hydroxide in preventing pain in necrotic dental pulps is so controversial, then a double blind study was carried out with80 patients who required endodontic treatment in non-vital teeth. it was observed that the frequency of postoperative pain exacerbation was relatively low, with significance index of 0,00018. this allows us to reach the conclusion that calcium hydroxide as a treatment in the prevention of pain in necrotic pulps interappointments does not avoid postoperative symptomatology because there are other factors that should be taken into account and although they are under control within the treatment, it is possible that they influence over the occurrence of interappointment pain.
Complex bioclimatic and soil gradients shape leaf trait variation in Embothrium coccineum (Proteaceae) among austral forests in Patagonia Gradientes bioclimáticos y edáficos modelan la variación en caracteres foliares de Embothrium coccineum (Proteaceae) en los bosques australes de la Patagonia
CINTIA P SOUTO,ANDREA C PREMOLI,PETER B REICH
Revista chilena de historia natural , 2009,
Abstract: Patterns of trait variation may be adaptive when vary in relation to an environmental gradient. In particular, leaf traits can affect productivity and competitive ability. We identify patterns of leaf size and shape variation with environmental heterogeneity in one of the most widespread tree species within temperate South America: Embothrium coccineum (Proteaceae). We collected leaf specimens and composite soil samples from 35 populations between 38° and 55° S latitude in Patagonia, covering a wide range of mean annual precipitation (MAP) and mean annual temperature (MAT). At each location, we measured nine morphological traits, some of which were cross correlated hence we focus on a smaller number of representative traits. We hypothesized that leaf area (LA), dry mass (DM), and specific leaf area (SLA) would increase, and that leaf shape (SF) would be more elongated, with increasing temperature, precipitation, and soil nutrient availability. We also expected growing season climate to be more closely associated with leaf traits than mean annual metrics. We used bivariate and backward stepwise multiple regressions to analyse the dependence of morphological traits with climatic and edaphic metrics. LA and DM increased with increasing summer rainfall or winter temperature, as hypothesized. Opposite to our hypothesis, LA and DM decreased with increasing summer temperature suggesting that in terms of leaf size, E. coccineum may sense summer conditions largely as an increasing aridity stressful gradient. Surprisingly, SLA increased with increasingly warm or dry summers. SF was related positively to MAT and negatively to MAP, suggesting that under more benign western climate regimes E. coccineum leaves tend to be elongated. Across sites, LA and DM increased with soil organic carbon and available phosphorus, and decreased with soil nitrogen and exchangeable cations. The opposite pattern was observed for SLA. Biologically meaningful climate metrics and soil nutrient conditions are useful predictors for leaf size and structure in the widespread E. coccineum. The SLA patterns probably resulted from lower values in long lasting leaves, in addition to increasing soil nitrogen, so leaves in the south are thicker. Alternatively, it could be consequence from non-isometrical scaling of LA and DM, so larger leaves such as those under oceanic western climates have lower SLA. Patterns of multiple leaf trait variation along complex environmental gradients may become uncoupled from each other, differing from what is suggested in the literature for traits that vary along simp
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