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What makes a yeast nuclear pore?
Elena Porro
Genome Biology , 2000, DOI: 10.1186/gb-2000-1-2-reports0046
Abstract: An established protocol was used to obtain nuclear fractions of yeast highly enriched in NPCs. Proteins contained in these fractions were separated by high performance liquid chromatography, and column fractions were separated by SDS gel electrophoresis and protein staining. Each distinct band was excised from the gel, digested with trypsin and analyzed by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) or MALDI ion trap tandem mass spectroscopy, or by microsequencing. Measured masses or sequences of peptides were used to identify corresponding open reading frames (ORFs) unambiguously from the yeast genome database. The authors claim that, by using large amounts of material and oversampling, they have identified all the proteins in the NPC fraction. To find the nups among the 174 identified proteins, the genes were first genomically epitope-tagged by insertion of a sequence within the genomic DNA that encodes a protein tag that can be identified later with an antibody. This ensured endogenous levels of expression and standardized detection in the subsequent characterization. Three criteria were used to determine whether the tagged proteins localized to the NPC: immunofluorescence localization to the nuclear rim; co-localization to the NPC in an NPC clustering assay; and biochemical fractionation to the nuclear envelope. This analysis identified 40 NPC proteins - 29 nups and 11 transport proteins, including three previously uncharacterized ORFs and four known proteins that had not been previously classified as nups. No motor proteins, ATPases, GTPases, or potential gate proteins were found. Further characterization of the nups localized the tagged proteins within the NPC by immunoelectron microscopic mapping onto a higher-resolution structure. Most nups were found on both the cytoplasmic and nuclear faces of the NPC. Two were exclusively nucleoplasmic, three exclusively cytoplasmic and four were found on both sides but showed a consistent bias
A Amaz?nia indígena de George Catlin: imagens e relatos de viagem desconhecidos
Porro, Antonio;
Boletim do Museu Paraense Emílio Goeldi. Ciências Humanas , 2010, DOI: 10.1590/S1981-81222010000300006
Abstract: first presentation and brazilian ethnohistory focused discussion of the 1852 amazonian travels and pictures of george catlin (1796-1872), and of their contribution to the knowledge of several brazilian indian tribes. the fame of catlin in the u. s. depended on his previous north american hundreds of paintings, with no consideration of his later south american pictures and memoirs here described. furthermore, catlin is not even mentioned in brazilian studies of xixth century traveler artists. the relevant passages of his memoirs are here translated and some of his many amazonian scenes and indians portraits are reproduced.
Arte e simbolismo xamanico na Amaz?nia
Porro, Antonio;
Boletim do Museu Paraense Emílio Goeldi. Ciências Humanas , 2010, DOI: 10.1590/S1981-81222010000100009
Abstract: although known for over a century, the so-called amazonian stone idols still represent a category of indigenous artifacts without archaeological, historical and ethnographic context. this article represents the first formal assessment of the known samples and presents hypotheses about their function and purpose. the style, symbolic patterns, and functional details of paired holes existing in all samples suggest their association to the ritual snuff of paricá (yakee) and other hallucinogenic substances by ancient amazonian shamans.
Uma cr?nica ignorada: Anselm Eckart e a Amaz?nia setecentista
Porro, Antonio;
Boletim do Museu Paraense Emílio Goeldi. Ciências Humanas , 2011, DOI: 10.1590/S1981-81222011000300007
Abstract: the almost unknown "additional notes on the description of the lands of brazil", by german jesuit anselm eckart (1721-1809), deserves great interest for amazonian studies. eckart, who joined for five years (1753-1757) the maranh?o and gr?o-pará missions, published his own observations appended to another author's book on amazonian natural and social world. the "additional notes" chapters concerning indian tribes' customs and situation under colonial rule are here translated by thekla hartmann and are commented by the author.
Notas sobre o antigo povoamento indígena do alto Trombetas e Mapuera
Porro, Antonio;
Boletim do Museu Paraense Emílio Goeldi. Ciências Humanas , 2008, DOI: 10.1590/S1981-81222008000300008
Abstract: this is the first geographical and ethnohistorical analysis of the 1728 narrative of friar francisco de s?o man?os on the first expedition to the trombetas and mapuera rivers in northwestern pará. the relevance of this document consists in the naming and approximate location of near 50 indian 'nations', most of them not mentioned by later historical and ethnographical sources, including many names of their villages and local chiefs. the reconstruction of the expedition's course showed that s?o man?os, known as the "trombetas' discoverer", had in fact traveled mostly through the mapuera, its western tributary, up to the guyana highlands. outstanding in the author's scarce ethnographic information, there are references to a two levels political chieftainship, and to war as means to capture slaves targeted to trade for goods.
Il corpo, lo sport, le immagini
Nicola Porro
[email protected]@ , 2009,
Abstract: E’ stato per primo Norbert Elias, nella sua critica al riduzionismo sociologico, a lamentare la difficoltà delle scienze sociali a misurarsi con le problematiche dello sport, così come delle emozioni, della sessualità, del gioco e della guerra. Attività umane che convergono in quell’autentico luogo sociale che è il corpo. Nel caso che ci interessa, si tratta di connettere il corpo, nella sua dimensione biologica, psichica e sociale, con quell’aspetto cruciale della civilizzazione che Elias definisce sportivizzazione.
Por qué los estudiantes de secundaria no eligen química como carrera universitaria y qué podría hacerse desde la universidad?
Silvia Porro
Química Viva , 2007,
Abstract:
The native Amazonia of George Catlin: little known pictures and travel accounts
Antonio Porro
Boletim do Museu Paraense Emílio Goeldi. Ciências Humanas , 2010,
Abstract: First presentation and Brazilian ethnohistory focused discussion of the 1852 Amazonian travels and pictures of George Catlin (1796-1872), and of their contribution to the knowledge of several Brazilian Indian tribes. The fame of Catlin in the U. S. depended on his previous North American hundreds of paintings, with no consideration of his later South American pictures and memoirs here described. Furthermore, Catlin is not even mentioned in Brazilian studies of XIXth century traveler artists. The relevant passages of his memoirs are here translated and some of his many Amazonian scenes and Indians portraits are reproduced.
Art and Shamanistic symbols in the Amazon
Antonio Porro
Boletim do Museu Paraense Emílio Goeldi. Ciências Humanas , 2010,
Abstract: Although known for over a century, the so-called Amazonian stone idols still represent a category of indigenous artifacts without archaeological, historical and ethnographic context. This article represents the first formal assessment of the known samples and presents hypotheses about their function and purpose. The style, symbolic patterns, and functional details of paired holes existing in all samples suggest their association to the ritual snuff of paricá (yakee) and other hallucinogenic substances by ancient Amazonian shamans.
The unpublished 1719 Report of Jacinto de Carvalho on Amazonian ethnography
Antonio Porro
Boletim do Museu Paraense Emílio Goeldi. Ciências Humanas , 2012,
Abstract: After working for thirteen years at the Amazonian Jesuitic missions, the Portuguese Jacinto de Carvalho (1677-1744) sent to the Superior of his order a report on the country and on some of its native tribes' customs. This report, the main ethnographic source of early 18th Century Amazonia, is known only through a coeval Italian translation still unpublished. In this paper, the parts on ethnographic matters have been commented and translated to Portuguese.
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