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Bird Diversity Relative to Forest Types and Physical Factors at Tung Salang Luang National Park, Thailand
Auttpol Nakwa,Narit Sitasuwan,Araya Jatisatein,Porntip Chantaramongkol
Research Journal of Biological Sciences , 2008,
Abstract: A survey of bird diversity was carried out at Tung Salang Luang National Park in three forest types i.e. mixed forest (seasonal evergreen forest mixed with deciduous dipterocarp forest), seasonal evergreen forest and deciduous dipterocarp forest, during March 2004 to February 2005. The point count mixed line transect methods were used for data collection. The survey found 6,697 birds in total from 140 sp., 35 families and 11 orders occurring in the mixed forest, seasonal evergreen forest and deciduous dipterocarp forest were as follows: 107, 100 and 94 sp. The quantitative bird communities have a negative correlation with climatic changes, as a result, the dynamic pattern of bird populations in the 3 habitats during a year were similar. The fewest species numbers and individual numbers were found during the rainy season and slightly high during the late rainy to early cool seasons. The highest bird populations were found during cool season. Similarity index values of birds in both mixed forest and seasonal evergreen forest were the greatest similar, while both mixed forest and deciduous dipterocarp forest were fewest less similar. The 72.6-78.3% qualitative similarity index values of bird species between study sites was done. Mixed forest had the highest Shannon diversity index 3.9507, followed by deciduous dipterocarp and seasonal evergreen forest were 3.6387 and 3.6025, respectively. The pattern observed suggest that the structure and dynamics of the Tung Salang Luang bird community are strongly liked to physical factors and habitat heterogeneity. Two particular species of bird were observed in this study: Aviceda jerdoni (Jerdon` Baza) and Coracina javensis (Javan Cuckooshrike).
Bird Diversity Relative to Forest Types and Physical Factors at Tung Salang Luang National Park, Thailand
Auttpol Nakwa,Narit Sitasuwan,Araya Jatisatein,Porntip Chantaramongkol,Wasun Pupichit,Pornchai Srisakb
Research Journal of Biological Sciences , 2012,
Abstract: A survey of bird diversity was carried out at Tung Salang Luang National Park in three forest types i.e. mixed forest (seasonal evergreen forest mixed with deciduous dipterocarp forest), seasonal evergreen forest and deciduous dipterocarp forest, during March 2004 to February 2005. The point count mixed line transect methods were used for data collection. The survey found 6,697 birds in total from 140 sp., 35 families and 11 orders occurring in the mixed forest, seasonal evergreen forest and deciduous dipterocarp forest were as follows: 107, 100 and 94 sp. The quantitative bird communities have a negative correlation with climatic changes, as a result, the dynamic pattern of bird populations in the 3 habitats during a year were similar. The fewest species numbers and individual numbers were found during the rainy season and slightly high during the late rainy to early cool seasons. The highest bird populations were found during cool season. Similarity index values of birds in both mixed forest and seasonal evergreen forest were the greatest similar, while both mixed forest and deciduous dipterocarp forest were fewest less similar. The 72.6-78.3% qualitative similarity index values of bird species between study sites was done. Mixed forest had the highest Shannon diversity index 3.9507, followed by deciduous dipterocarp and seasonal evergreen forest were 3.6387 and 3.6025, respectively. The pattern observed suggest that the structure and dynamics of the Tung Salang Luang bird community are strongly liked to physical factors and habitat heterogeneity. Two particular species of bird were observed in this study: Aviceda jerdoni (Jerdon` Baza) and Coracina javensis (Javan Cuckooshrike).
Effectiveness of Ringworm Cassia and Turmeric Plant Extracts on Growth Inhibition against Some Important Plant Pathogenic Fungi  [PDF]
Porntip Wongkaew, Wantana Sinsiri
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.55076
Abstract:

Crude plant extracts of ringworm cassia, Cassia alata L. and turmeric, Curcuma longa L. were prepared by either hot water or organic solvents such as ethanol and ether. Various concentrations of the crude extract were then subjected to an in vitro test for their effectiveness on mycelia growth inhibition against some important plant pathogenic fungi such as Alternaria alternata, Colletotrichum gloeosporioides, Fusarium oxysporum fsp. lycopersici, Sclerotium rolfsii, Phytophthora infestans and Pythium sp. in comparison to commercial fungicides such as copper oxychloride and mancozeb. Reduction of the fungal growth was significantly obtained with C. longa extracts and the best median effective inhibitory concentration (IC50) value of 6.07, 6.50 and 7.13 mg/ml was from the ethanol extract for S. rolfsii, C. gloeosporioides and F. oxysporum fsp. lycopersici respectively. While all extracts from C. alata

Diagnosis of Sugarcane White Leaf Disease Using the Highly Sensitive DNA Based Voltammetric Electrochemical Determination  [PDF]
Porntip Wongkaew, Suta Poosittisak
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.515240
Abstract:

A simple and highly sensitive analysis by electrochemical voltammetry has been developed for diagnosis of the most destructive crop disease in Thailand known as sugarcane white leaf (SCWL). Determination of the corresponding DNA interaction has been obtained from the voltammetric signals of electroactive redox methylene blue (MB) by means of cyclic and differential pulse voltammetry. In this study, a chitosan-modified glassy carbon electrode (GCE) was created by self-assembly to produce electrostatic platform for effective immobilization of the DNA. Fabrication of SCWL-DNA hybridization detection system was performed by immobilizing the ssDNA probe as a specific sensor onto chitosan-modified GCE. Hybridization of complementary DNA from the real samples could then be detected by its respective MB signal. This fabricated DNA probe sensor was shown to be capable for discriminative identification among the DNAs from SCWL plants, mosaic virus infected sugarcane and healthy sugarcane plants. Relationship between the specific hybridization signal and DNA target concentration was also observed under optimal condition. The detection limit of 4.709 ng/μl with the regression coefficient (R2) of 0.998 and overall RSD of 2.44% were obtained by response curve fit analysis. The actual SCWL-ssDNA immobilization and hybridizing event were subsequently confirmed by an observation under atomic force microscope. Thus these experiments demonstrate the first successful and effective DNA based voltammetric electrochemical determination for a verification of the specific pathogenic infection within plants from the real epidemic field.

Pretreatment Evaluation and its Application on Palm Oil Mill Effluent for Bio-Hydrogen Enhancement and Methanogenic Activity Repression
Porntip Wimonsong,Suwanchai Nitisoravut
Pakistan Journal of Biological Sciences , 2009,
Abstract: Pretreatment evaluations of biological sludge were performed to observe the enhancement of hydrogen production and repression of methanogenic activity using anaerobic sludge and sucrose as substrate. The treatments include heating (H), ozonation (O), drug (D) application using fluvastatin, Na2SO4(S) dosing and their combinations to make up for the total of 9 pretreatment methods. Heat treatment at 95°C for 45 min provided a complete methanogen repression and good enhancement of hydrogen production activity. The remaining pretreatments were imperfect either for repression or enhancement based on the application conditions and concentration ranges. The order of methane repression was: H>HO>HD>HS>O>D>OD>OS>S while hydrogen production was: HO>H>HS>OD>HD>OS>D>O>S. Heat treatment at the specified condition was considered sufficient and suitable for pretreatment of anaerobic sludge. The practical application was explored using Palm Oil Mill Effluent (POME) containing different amounts of solid content (15.830 g VSS L-1, namely LPOME and 21.445 g VSS L-1, namely HPOME) as substrate. Beside the difference in COD of nearly 16% which is higher for HPOME, a higher specific hydrogen production rate was obtained at 0.81 and 0.17 mL H2 g-1 COD h for LPOME and HPOME, respectively. It should also be noted that a prolonged lag-time during start-up was observed for HPOME as well as hydrogen suppression which may be associated with the solid content mainly lipids in wastewater. Thus, high solid content of wastewater may be of concerned for bio-hydrogen production.
Use of an infrared thermometer for assessment of plant water stress in neck orange (Citrus reticulata Blanco)
Sayan Sdoodee,Porntip Kaewkong
Songklanakarin Journal of Science and Technology , 2006,
Abstract: In general, water stress causes stomatal closure in citrus, and this leads to higher leaf temperature. Recently, it has been reported that infrared thermometry technique can be used for detecting stomatal closure indirectly to assess plant water stress. Therefore, it was proposed to apply to neck orange. An experiment was arranged as a completely randomized design. There were 3 treatments of watering levels: 1) wellwatering (W1), 2) 3-day interval watering (W2), and 3) 6-day interval watering (W3) with 6 replicates. Eighteen 2-year-old trees of neck orange were used, and each tree was grown in a container (0.4 m3) filled with mixed media of soil, compost and sand (1:1:1). During 18 days of the experimental period, it was found that leaf water potential and stomatal conductance of the plants in W2 and W3 treatments decreased with the progress of water stress. There was high correlation (r2 = 0.71**) between leaf water potential and stomatal conductance as a linear regression (Y = 0.0044X-1.8635). Canopy temperature (Tc) and air temperature (Ta) of each tree were measured by an infrared thermometer, and Tc-Ta was assessed. At the end of the experimental period, it was found that Tc-Ta was significantly highest in the W3 treatment (3.5oC) followed by the of W2 treatment (2oC), while it was lowest in the W1 treatment (1oC). The relationship between Tc-Ta and stomatal conductance was high as polynomial (Y = 0.0002X2 0.0572X+3.9878, r2 = 0.70**). This indicated that stomatal closure or decreasing stomatal conductance caused increasing of Tc-Ta in the leaves. Hence, it suggests that infrared thermometer is a convenient device for the assessment of plant water stress in neck orange.
Agrobacterium-Mediated Transformation of Dendrobium secundum (Bl.) Lindl with Antisense ACC Oxidase
Porntip Atichart,Sumontip Bunnag,Piyada Theerakulpisut
Asian Journal of Plant Sciences , 2007,
Abstract: The suitable medium which resulted in 98% for seed germination was the Vacin and Went (1949) medium supplemented with 100 mg L-1 myo-inositol, 1 mg L-1 thiamine, 1 mg L-1 nicotinic acid, 1 mg L-1 pyridoxine and 4 mg L-1 glycine, 15% coconut water and 0.8% agar at pH 5.4 under light condition. Protocorms with high proliferation capacity were cultured on the modified VW medium and 0.5 mg L-1 NAA. Genetics transformation of Dendrobium secundum mediated by Agrobacterium tumefaciens strain LBA 4404, which harbored the plasmid pCAMBIA 1305.1 containing antisense ACC oxidase, β-glucuronidase (GUS) and hygromycin resistance (hptII) genes. The efficiency of transformation was 40 min co-cultivation of protocorms with A. tumefaciens that has been induced with 200 μΜ acetosyringone. An experiment was conducted to determine the effect of antibiotic on protocorm growth. It was found that hygromycin at 25 mg L-1 was effective for transformant selection. The maximum concentration of cefotaxime that protocorm could tolerate was 500 mg L-1. Hygromycin resistant protocorms showed histochemical blue staining due to GUS activity was 60%. Successful the transformation was confirmed by PCR analysis. It was found that the sizes of amplified fragments were 195, 180 and 843 bp for the 35S, NOS and antisense ACC oxidase, respectively.
An improved method for concentrating rotavirus from water samples
Kittigul Leera,Khamoun Porntip,Sujirarat Dusit,Utrarachkij Fuangfa
Memórias do Instituto Oswaldo Cruz , 2001,
Abstract: A modified adsorption-elution method for the concentration of seeded rotavirus from water samples was used to determine various factors which affected the virus recovery. An enzyme-linked immunosorbent assay was used to detect the rotavirus antigen after concentration. Of the various eluents compared, 0.05M glycine, pH 11.5 gave the highest rotavirus antigen recovery using negatively charged membrane filtration whereas 2.9% tryptose phosphate broth containing 6% glycine; pH 9.0 was found to give the greatest elution efficiency when a positively charged membrane was used. Reconcentration of water samples by a speedVac concentrator showed significantly higher rotavirus recovery than polyethylene glycol precipitation through both negatively and positively charged filters (p-value <0.001). In addition, speedVac concentration using negatively charged filtration resulted in greater rotavirus recovery than that using positively charged filtration (p-value = 0.004). Thirty eight environmental water samples were collected from river, domestic sewage, canals receiving raw sewage drains, and tap water collected in containers for domestic use, all from congested areas of Bangkok. In addition, several samples of commercial drinking water were analyzed. All samples were concentrated and examined for rotavirus antigen. Coliforms and fecal coliforms (0->1,800 MPN/100 ml) were observed but rotavirus was not detected in any sample. This study suggests that the speedVac reconcentration method gives the most efficient rotavirus recovery from water samples.
Annexin A1: A new immunohistological marker of cholangiocarcinoma
Nuttanan Hongsrichan,Rucksak Rucksaken,Yaovalux Chamgramol,Porntip Pinlaor
World Journal of Gastroenterology , 2013, DOI: 10.3748/wjg.v19.i16.2456
Abstract: AIM: To evaluate a new immunohistological marker, annexin A1 (ANXA1), in cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC). METHODS: Expression of ANXA1 protein was investigated in liver tissues from patients with CCA and HCC by immunohistochemistry. Its expression on differences stages of tumor development was investigated in hamster CCA tissues induced by Opisthorchis viverrini and N-nitrosodimethylamine. Moreover, mRNA expression of ANXA1 was assessed in CCA cell lines by quantitative real-time polymerase chain reaction and silencing of ANXA1 gene expression using small interfering RNA. RESULTS: In human CCA tissue arrays, immunohistochemical analysis revealed that the positive expression of ANXA1 was 94.1% (64/68 cases) consisting of a high expression (66.2%, 45/68 cases) and a low expression (33.8%, 23/68 cases). However, expression of ANXA1 protein was negative in all histologic patterns for HCC (46/46 cases) and healthy individuals (6/6 cases). In hamster with opisthorchiasis-associated CCA, the expression of ANXA1 was observed in the cytoplasm of inflammatory cells, bile duct epithelia and tumor cells. Grading scores of ANXA1 expression were significantly increased with tumor progression. In addition, mRNA expression of ANXA1 significantly increased in all of the various CCA cell lines tested compared to an immortalized human cholangiocyte cell line (MMNK1). Suppressing the ANXA1 gene significantly reduced the matrix metalloproteinase (MMP) 2 and MMP9, and transforming growth factor-β genes, but increased nuclear factor-κB gene expression. CONCLUSION: ANXA1 is highly expressed in CCA, but low in HCC, suggesting it may serve as a new immunohistochemical marker of CCA. ANXA1 may play a role in opisthorchiasis-associated cholangiocarcinogenesis.
The Effects of Tourists on Bird Diversity in Tourist Area Compared to Restricted Area of Seasonal Evergreen Forest at Tung Salang Luang National Park, Phetchabun Province, Thailand
Auttpol Nakwa,Narit Sitasuwan,Araya Jatisatein,Porntip Chantaramongko
International Journal of Zoological Research , 2008,
Abstract: A survey of bird similarity, diversity and density were carried out at Tung Salang Luang National Park during March 2004 - February 2005, in 3 sites of seasonal evergreen forest, one site in a restricted area (SE1) and two sites in tourist areas (SE2 and SE3). Three sites were located in the same forest structure. The point count and line transect methods were used for data collection. The results revealed the following information: 133 species, 34 families and 11 orders of birds in SE1 (102 species), SE2 (100 species) and SE3 (89 species) were observed. Seven species of birds in all sites i.e. Criniger pallidus, Hypsipetes propinquus, Pycnonotus melanicterus, Irena puella, Garrulax leucolophus, Yuhina zantholeuca and Gracula religiosa were the co-dominant species in this forest that will be used indicator for future investigation. Base on similarity, tourist activities may be disturbed some bird groups in tourist area such as carnivorous and omnivorous (SE2 and SE3) and nectarivorous (SE3); base on densities, carnivorous (SE3), nectarivorous (SE2 and SE3) and garnivorous (SE3) were decreased 46-78 % in tourist sites compared with restricted site (SE1). Moreover, bird diversity index in restricted area was higher value than tourist area. This phenomenon indicated that some bird groups in tourist area at the seasonal evergreen forest had negative effect correlation with human activities and similarity, diversity and density indices were a proper indicator for further impact investigation for conservation and management strategies of avifauna. Finally, this result was the first report about avifauna dynamic of Tung Salang Luang National Park.
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