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OALib Journal期刊

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CGAR E CGAR-EM na análise dos constituintes químicos isolados do extrato hexanico de Sebastiania argutidens (Euphorbiaceae)
Branco, Alexsandro;Pizzolatti, Moacir G.;
Química Nova , 2002, DOI: 10.1590/S0100-40422002000100004
Abstract: the fractionation column with sio2 of the hexane extract of sebastiania argutidens (euphorbiaceae) yielded fractions containing hydrocarbons, carboxylic acids, sterols and pentacyclic triterpenes. besides, one fraction showed the presence of several methyl esters, including four uncommon long chain palmitate esthers as minor components. the characterization of these chemical constituents have been done by high resolution gas chromatography (hrgc) and hrgc coupled to mass spectrometry (gc/ms). campesterol, stigmasterol, b-sitosterol, glutin-5-en-3-ol were identified by hrgc co-injection with standards.
CGAR E CGAR-EM na análise dos constituintes químicos isolados do extrato hexanico de Sebastiania argutidens (Euphorbiaceae)
Branco Alexsandro,Pizzolatti Moacir G.
Química Nova , 2002,
Abstract: The fractionation column with SiO2 of the hexane extract of Sebastiania argutidens (Euphorbiaceae) yielded fractions containing hydrocarbons, carboxylic acids, sterols and pentacyclic triterpenes. Besides, one fraction showed the presence of several methyl esters, including four uncommon long chain palmitate esthers as minor components. The characterization of these chemical constituents have been done by High Resolution Gas Chromatography (HRGC) and HRGC coupled to Mass Spectrometry (GC/MS). Campesterol, stigmasterol, b-sitosterol, glutin-5-en-3-ol were identified by HRGC co-injection with standards.
Strategy for a Fast and Simple Method for Trace Determination of Senecionine and Senecionine N-Oxide in Honey Using LVI in HPLC-MS/MS  [PDF]
Lizandra Czermainski Bretanha, Marcel Piovezan, Alysson Fernandez Vasques Sako, Moacir Geraldo Pizzolatti, Gustavo Amadeu Micke
American Journal of Analytical Chemistry (AJAC) , 2014, DOI: 10.4236/ajac.2014.510076
Abstract: The aims of this study were to develop an HPLC-MS/MS method for the trace determination of senecionine (SEN) and senecionine N-oxide (SENNOX) in honey samples with simple dilution and to optimize the strategy for large volume injection (LVI). In the method development, the quantity of sugar, injection volume and sample pH were optimized to increase the efficiency and the retention factor of the analytes. Figures of merit of the proposed method include: good linearity (R2 > 0.99), limits of detection of 57 and 59 ng·kg﹣1 for SEN and SENNOX, respectively, and recovery in the range of 83.4% - 123%. The values for the instrumental precision in relation to the retention time and peak area were 0.65% and 7.1%, respectively, and for the intra-day precision they were greater than 0.95% and 9.7% for SEN and SENNOX, respectively. The LVI-HPLC-MS/MS procedure developed was applied to honey samples prepared by simple dilution with deionized water (1:8 w/w) and the pH was adjusted to 9.5 with NH4OH. The sample volume injected was 100 μL; concentrations in natural and industrialized honey were ﹤LOQ - 1071.3 μg·kg﹣1 for SEN and ﹤LOD - 16.9 μg·kg﹣1 for SENNOX.
Gênero Baccharis (Asteraceae): aspectos químicos, econ?micos e biológicos
Verdi, Luiz Gonzaga;Brighente, Inês Maria Costa;Pizzolatti, Moacir Geraldo;
Química Nova , 2005, DOI: 10.1590/S0100-40422005000100017
Abstract: the baccharis genus is represented by more than 500 species distributed mainly in the tropical areas of south america. many of them are extensively used in folk medicine in the treatment or prevention of anemias, inflammations, diabetes and stomach, liver and prostate diseases. phytochemical and biological investigations in about 120 species resulted mainly in the isolation of clerodane and labdane diterpenes and flavonoid aglicones with the flavone unit being the most frequent.
Evaluation of the antiinflammatory efficacy of Lotus pedunculatus
Pereira Diana,Koelzer Janaina,Dalmarco Juliana,Pizzolatti Moacir
International Journal of Green Pharmacy , 2009,
Abstract: The aim of this study was to investigate the antiinflammatory effect of the crude extract of Lotus pedunculatus. Aerial parts of Lotus pedunculatus were extracted by maceration with ethanol 96%. The solvent was evaporated under low pressure at 55°C to dryness, yielding crude extract. The antiinflammatory effects of Lotus pedunculatus were analyzed using carrageenan-induced mouse pleurisy. Significance was determined by analysis of variance, Dunnett′s or Student′s t -tests. Values of P < 0.05 were considered significant. The crude extract of Lotus pedunculatus inhibited leukocyte, neutrophil, myeloperoxidase, and adenosine-deaminase activities, as well as exudation, nitric oxide, interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels ( P < 0.05). These results indicate that Lotus pedunculatus has an important antiinflammatory action and its inhibitory activity may be due to not only to the inhibition of proinflammatory mediators, but also to the inhibition of the activated leukocytes.
Isolation and identification of bioactive compounds responsible for the anti-bacterial efficacy of Lotus corniculatus var. S o Gabriel
Dalmarco Juliana,Dalmarco Eduardo,Koelzer Janaina,Pizzolatti Moacir
International Journal of Green Pharmacy , 2010,
Abstract: Lotus corniculatus (Fabaceae) is considered a forage plant utilized as food for ruminants in the south of Brazil. This herb is also actually used to treat intestinal infection in these animals. In our experiments, we evaluated the anti-bacterial activity of crude extract from L. corniculatus var. Sγo Gabriel were assayed against Gram-positive and Gram-negative bacterium. The crude extracted did not show any anti-bacterial activity, but the hexane fraction did on Bacillus cereus (MIC=100 μg/mL) and on Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, Acinetobacter calcoaceticus, and Providencia alcalifaciens (MIC=600, 800 or 1000 μg/mL). The Ethyl acetate fraction (AcOEt) also showed important anti-bacterial activity on Bacillus cereus, E. faecalis, and Acinetobacter calcoaceticus (MIC=800 μg/mL). The oleanolic acid isolated from hexane fraction showed the same effect on Staphylococcus aureus methycillin-resistant (MIC=100 μg/mL), L. monocytogenes (MIC=25 μg/mL), and Bacillus cereus (MIC=25 μg/mL). Further, Kaempferitrin isolated from ethyl acetate fraction has also shown anti-bacterial activity on Shighella flexinerii (MIC=100 μg/mL), Salmonella typhimurium (MIC=100 μg/mL), A. calcoaceticus (MIC=100 μg/mL), E. faecalis (MIC=3.9 μg/mL), and Bacillus cereus (MIC=8.5 μg/mL). This study suggests that L. corniculatus var. Sγo Gabriel have potential pharmacological property for a new anti-bacterial drug development.
Activation of Human Neutrophils by the Anti-Inflammatory Mediator Esenbeckia leiocarpa Leads to Atypical Apoptosis
Rafael de Liz,Heros Horst,Moacir Geraldo Pizzolatti,Tania Silvia Fr de,Denis Girard
Mediators of Inflammation , 2012, DOI: 10.1155/2012/198382
Abstract: Despite the fact that Esenbeckia leiocarpa, a Brazilian plant, possesses potential anti-inflammatory properties, its effect in neutrophils, key players in inflammation, has never been investigated. In this study, a crude hydroalcoholic extract (CHE) was used to evaluate the potential toxic or agonistic effect of E. leiocarpa in human neutrophils. At a noncytotoxic concentration of 500 μg/mL, CHE increased actin polymerization and cell signaling events, especially p38 MAPK. Its modulatory activity on neutrophil cell apoptosis was investigated by cytology and by flow cytometry and, although CHE increased the apoptotic rate (by cytology) and increased annexin-V binding, it did not, unexpectedly, increase CD16 shedding. CHE increased the degradation of the cytoskeletal proteins gelsolin and paxillin but, surprisingly, not of vimentin. The proapoptotic activity of CHE was reversed by a pan-caspase inhibitor but not by a p38 inhibitor. We conclude that CHE is a novel human neutrophil agonist that induces apoptosis by a caspase-dependent and p38-independent mechanism in an atypical fashion based on its lack of effect on CD16 shedding and vimentin degradation. Since the resolution of inflammation occurs by elimination of apoptotic neutrophils, the ability of CHE to induce neutrophil apoptosis correlates well with its anti-inflammatory properties, as previously reported.
Activation of human neutrophils by Esenbeckia leiocarpa: comparison between the crude hydroalcoholic extract (CHE) and an alkaloid (Alk) fraction
Rafael de Liz, Heros Horst, Moacir Geraldo Pizzolatti, Tania Silvia Fr?de, Denis Girard
Journal of Inflammation , 2012, DOI: 10.1186/1476-9255-9-19
Abstract:
Antifungal activity of five species of Polygala
Johann, Susana;Mendes, Beatriz G;Missau, Fabiana C;Resende, Maria A. de;Pizzolatti, Moacir G;
Brazilian Journal of Microbiology , 2011, DOI: 10.1590/S1517-83822011000300027
Abstract: crude extracts and fractions of five species of polygala - p. campestris, p. cyparissias, p. paniculata, p. pulchella and p. sabulosa - were investigated for their in vitro antifungal activity against opportunistic candida species, cryptococcus gattii and sporothrix schenckii with bioautographic and microdilution assays. in the bioautographic assays, the major extracts were active against the fungi tested. in the minimal concentration inhibitory (mic) assay, the hexane extract of p. paniculata and etoac fraction of p. sabulosa showed the best antifungal activity, with mic values of 60 and 30 μg/ml, respectively, against c. tropicalis, c. gattii and s. schenckii. the compounds isolated from p. sabulosa prenyloxycoumarin and 1,2,3,4,5,6-hexanehexol displayed antifungal activity against s. schenckii (with mics of 125 μg/ml and 250 μg/ml, respectively) and c. gattii (both with mics of 250 μg/ml). rutin and aurapten isolated from p. paniculata showed antifungal activity against c. gattii with mic values of 60 and 250 μg/ml, respectively. in the antifungal screening, few of the isolated compounds showed good antifungal inhibition. the compound α-spinasterol showed broad activity against the species tested, while rutin had the best activity with the lowest mic values for the microorganisms tested. these two compounds may be chemically modified by the introduction of a substitute group that would alter several physico-chemical properties of the molecule, such as hydrophobicity, electronic density and steric strain.
Antifungal properties of plants used in Brazilian traditional medicine against clinically relevant fungal pathogens
Johann, Susana;Pizzolatti, Moacir G.;Donnici, Cláudio L.;Resende, Maria Aparecida de;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000400010
Abstract: antifungal properties of extracts from eight brazilian plants traditionally used in popular brazilian medicine were tested against five clinically relevant candida species, cryptococcus neoformans, and sporothrix schenckii. results demonstrate that almost all extracts exhibited antifungal activity, at least against one of the microorganisms tested. the ethanolic extract from the leaves of schinus terebinthifolius exhibited potential antifungal activity against c. glabrata and s. schenckii. preliminary phytochemical analysis of extract from s. terebinthifolius showed the presence of biologically active compounds, namely saponins, flavonoids, triterpenes, steroids and tannins.
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