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Polimorfismo isoenzimático en cuatro razas y un híbrido de Bactris gasipaes (Palmae)
Rojas-Vargas,Sonia; Ramírez,Pilar; Mora-Urpí,Jorge;
Revista de Biología Tropical , 1999,
Abstract: the study of genetic diversity in peach palm (bactris gasipaes k.) is important for the breeding work on this palm and to corroborate the hypotheses on its origins. for that purpose it is necessary to use alternative techniques to complement the morphological studies traditionally made. one of the techniques that responds to that need is isozyme electrophoresis. the isozymes are biochemical markers of importance in the study of genetic variability in plants of economic importance, because they are the primary products of genetic expression. this work is an electrophoretic analysis on gels of polyacrilamide to study phenetic relations using twelve isozymes on four races and an spontaneous hybrid of peach palm. the biological material used came from the germplasm bank from los diamantes experimental station, guápiles-costa rica. four races and a hybrid were selected from that collection. they represented different procedences with morphological differences as well as having particular geographical distribution patterns; they were: tembé-chapare (bolivia-bo), pará -belem (brasil-bra), utilis guápiles (costa rica-cr), tuira-darién (panamá-pa) and yurimaguas (perú-pe). five trees of each race and the yurimaguas hybrid were studied. different tissues were tried: leaf, adventitious roots, spines, masculine flowers and ripe fruits. an standard native and discontinued method was established on a polyacrilamide matrix for the analysis of twelve isozymes: (prx, est, acp, dia, g6pdh, me, mdh, got, adh, pgi, pgm and sod). the amount of tissue used was 1. 5 g, with 20 min clarification time at 15000 gravity value and a temperature of 4°c. the protocol proposed by baaziz & saaidi (1988) was followed with a modification of concentration of the phosphate buffer solution from 0,005 m to 0. 1m with a 50 to 200 volts gradient. the root, flower and spine tissue were difficult to homogenize because of their high fiber content. the fruit has too much oil and starch, which distort the band
Polimorfismo isoenzimático en cuatro razas y un híbrido de Bactris gasipaes (Palmae)
Sonia Rojas-Vargas,Pilar Ramírez,Jorge Mora-Urpí
Revista de Biología Tropical , 1999,
Abstract: Se estandarizó un sistema de electroforesis de isoenzimas con tejido de hojas que permitió la identificación del polimorfismo fenético entre cuatro razas y un híbrido de pejibaye Bactris gasipaes provenientes de Brasil, Perú, Bolivia, Panamá y Costa Rica. Las isoenzimas ensayadas fueron: PRX, EST, ACP, ME, DIA, MDH, G6PDH, PGI, SOD, PGM, ADH, GOT, de estas solamente las dos ltimas no mostraron actividad. El resto de isoenzimas mostraron polimorfismo fenético en diferentes grados, por esto se consideran marcadores polimórficos potenciales para estudios de variabilidad genética en pejibaye. Se encontró un locus único en el zimograma de la enzima PRX en las muestras de Utilis-Guápiles (CR), el cual podría usarse como un marcador discriminatorio para esta raza. Se establecieron relaciones de similitud isoenzimática entre las razas Utilis-Guápiles (CR) y Tuira-Darién (Pa); Tembé-Chapare (Bo) y Pará-Belem (Bra) respectivamente, mientras que el híbrido Yurimaguas (Pe) se ubicó aparte y ligeramente más cercano a Utilis y Tuira, por esto se considera que posiblemente desciende de padres aún no identificados. The study of genetic diversity in peach palm (Bactris gasipaes K.) is important for the breeding work on this palm and to corroborate the hypotheses on its origins. For that purpose it is necessary to use alternative techniques to complement the morphological studies traditionally made. One of the techniques that responds to that need is isozyme electrophoresis. The isozymes are biochemical markers of importance in the study of genetic variability in plants of economic importance, because they are the primary products of genetic expression. This work is an electrophoretic analysis on gels of polyacrilamide to study phenetic relations using twelve isozymes on four races and an spontaneous hybrid of peach palm. The biological material used came from the germplasm bank from Los Diamantes Experimental Station, Guápiles-Costa Rica. Four races and a hybrid were selected from that collection. They represented different procedences with morphological differences as well as having particular geographical distribution patterns; they were: Tembé-Chapare (Bolivia-Bo), Pará -Belem (Brasil-Bra), Utilis Guápiles (Costa Rica-CR), Tuira-Darién (Panamá-Pa) and Yurimaguas (Perú-Pe). Five trees of each race and the Yurimaguas hybrid were studied. Different tissues were tried: leaf, adventitious roots, spines, masculine flowers and ripe fruits. An standard native and discontinued method was established on a polyacrilamide matrix for the analysis of twelve isozymes: (PRX, EST, AC
FACTORES SOCIO-AFECTIVOS RELACIONADOS CON LAS DIFICULTADES ESCOLARES EN NI AS Y NI OS “ESTRELLA” DEL PROGRAMA PSICOMOTRICIDAD E INTERVENCIóN
Emmanuel Herrera González,Laura Delgado Tenorio,Héctor Fonseca Schmidt,Pilar Vargas Ramírez
Revista en Ciencias del Movimiento Humano y Salud , 2012,
Abstract: El presente estudio muestra los principales factores socio-afectivos relacionados con las dificultades escolares de un grupo de tres ni as y tres ni os identificados como “estrella” por el Programa Psicomotricidad e Intervención. El mismo se desarrolló a través de una metodología mixta, en la que se aplicaron el Test de Figura Humana, el Dibujo Kinético de la Familia y una entrevista a la madre de cada estudiante. Los resultados revelaron la existencia de factores conflictivos familiares que afectan el estado emocional de las y los ni os, generándoles sentimientos negativos consigo mismos que terminan afectado sus interacciones sociales y también su desempe o escolar.
Effect of Gamma Irradiation and Selection with Fungus Filtrate (Rhizoctonia solani Kuhn) on the in Vitro Culture of Common Bean (Phaseolus vulgaris)  [PDF]
Laura Y. Solís-Ramos, Marta Valdez-Melara, Ricardo Alvarado-Barrantes, Floribeth Mora-Uma?a, Eduardo Hernández-Jiménez, Natalia Barboza-Vargas, Pilar Ramírez-Fonseca
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.616269
Abstract: The present investigation was undertaken to study the effect of gamma irradiation (dose from 10 to 100 Gy) and in vitro selection with fungus filtrate as selecting agent (concentration from 20% to 100%) on the susceptibility of the common bean to Rhizoctonia solani. The best results were found with a dose of 20 Gy or a concentration of 20% of fungus filtrate applied separately. These conditions were used to evaluate the combined effect of both approaches in a second experiment. The combined effect of irradiation and then selection adversely affected growth (height and roots) and survival of the in vitro plants. It may not be necessary to combine the variation generated by irradiation with the selection technique. For future assays we propose the application of: 1) gamma radiation, thereby inducing not only mutants with pathogen resistance, but also with other agronomic traits of interest. Later in the subculture MV4 potential fungus-resistant mutants will be evaluated in the field; or 2) selection pressure using fungus filtrate during three subcultures, which may be sufficient to induce the variation necessary to obtain in vitro plants resistant to fungus.
Modelling Entrepreneurial Attitudes in Women Entrepreneurs with Bayesian Networks  [PDF]
Jorge López, Alicia Ramírez, Pilar Casado
Psychology (PSYCH) , 2012, DOI: 10.4236/psych.2012.33037
Abstract: The entrepreneurial attitude model is nowadays getting more attention as a framework to explain and describe new business creation. In short, the attitude model posits that the entrepreneurial behavior is a planed action conditioned by the potential entrepreneur’s attitudes which depends on desirability and feasibility beliefs. We have developed and compared three different Bayesian net models taking into account the principles of the Shapero’s entrepreneurial event model. We have also modeled feasibility using two different dimensions. Firstly, we considered opportunity feasibility dimension referring to the degree in which a business would be successful attending to the market opportunities or demands. Secondly, we included a dimension of resources feasibility referring to the feasibility of the business in terms of the availability of possible resources to make the company a reality. The first model contained both feasibility dimensions whereas the other two only contained one dimension. Our results show that the Bayesian model containing the two forms of feasibility is better to predict entrepreneurial intentions. Implications in the context of promoting entrepreneurial attitudes and behaviors amongst women are finally discussed.
Nuevos actores sociopolíticos en el escenario internacional.
Socorro Ramírez Vargas
Colombia Internacional , 1997,
Abstract:
Neurodesarrollo en infantes con antecedente de hipotiroidismo congénito
Arreóla-Ramírez, Gabriela;Barrera-Reyes, René Humberto;Jiménez-Quiroz, Rosalía;Ramírez Torres, María Aurora;Segura-Cervantes, Enrique;Granados-Cepeda, Martha Lucía;Ramírez-Vargas, Mayra Norma;Meza-Rodríguez, María del Pilar;
Perinatología y reproducción humana , 2005,
Abstract: introduction: follow-up studies of hypothyroid infants who received early treatment evidence the presence of the same iq vs. the general population. material and methods: retrospective cohort study. twenty-four hypothyroid infants detected in neonatal screening were compared to a control group. the newborns were assessed from six months to seven years based on bayley's infant development scales (id), the terman-merril iq (cl) and the learning aptitude of the heard of hearing with the hiskey-nebraska test (ca). results: twenty hypothyroid infants showed a normal or high cl or id. one of the infants with a id result below the mean, two with an cl below the mean, one hearing impaired infant with low learning aptitude. no differences were found vs. the control group. the use of ototoxic medications, prematurity and hyperbilirubinemia explain the low coefficients between the hypothyroid individuals and the control. conclusions: the cl and the id results in the hypothyroid group who received early treatment showed no difference vs. the control group.
El pensamiento integrado en el Currículo de una lengua extranjera
Pilar Morán Ramírez
Revista Signos , 1997,
Abstract:
Un método de transformación genética de maíz para conferirle resistencia ulterior a enfermedades virales
Valdez,Marta; Madriz,Kenneth; Ramírez,Pilar;
Revista de Biología Tropical , 2004,
Abstract: a method for genetic transformation of maize for resistance to viral diseases. a system for the genetic transformation of maize was developed for two costa rican varieties: cr-7 and diamantes 8843, that can allow the subsequent transfer of viral-derived genes in order to confer resistance to the disease caused by maize rayado fino virus (mrfv). the method is based on particle bombardment of organogenic calli derived from shoot tips. on the other hand, the molecular construction prfcp-bar, containing the coat protein gene of mrfv and the marker gene bar, was elaborated. for the visual selection of the transformed material was used also the plasmid pdm803 that contains the reporter gene uida (gus).the results indicate that devices evaluated: the pig (" particle inflow gun ") and the bio-rad ? are both enough efficient to transfer foreign genes to the genome of the maize. rev. biol. trop. 52(3): 787-793. epub 2004 dic 15.
Un método de transformación genética de maíz para conferirle resistencia ulterior a enfermedades virales
Marta Valdez,Kenneth Madriz,Pilar Ramírez
Revista de Biología Tropical , 2004,
Abstract: Se desarrolló un sistema de transformación genética para dos variedades costarricenses de maíz: CR-7 y Diamantes 8843, que permita la transferencia ulterior de genes de origen viral a su genoma, y conferirles resistencia a la enfermedad ocasionada por el virus del rayado fino del maíz (MRFV). El método se basa en el bombardeo de microproyectiles en callos organogénicos derivados de ápices de jóvenes vitrogerminaciones. Por otro lado, se elaboró la construcción molecular pRFcp-bar que contiene el gen de la cubierta proteica del MRFV y el gen marcador bar. Para la selección visual del material transformado, se utilizo también el plásmido pDM803 que contiene el gen reportero uidA (GUS). Los resultados indican que los dos aceleradores de partículas evaluados: el PIG ("Particle Inflow Gun") y el Bio-Rad son igualmente eficientes para transferir genes foráneos al genoma del maíz. A method for genetic transformation of maize for resistance to viral diseases. A system for the genetic transformation of maize was developed for two Costa Rican varieties: CR-7 and Diamantes 8843, that can allow the subsequent transfer of viral-derived genes in order to confer resistance to the disease caused by maize rayado fino virus (MRFV). The method is based on particle bombardment of organogenic calli derived from shoot tips. On the other hand, the molecular construction pRFcp-bar, containing the coat protein gene of MRFV and the marker gene bar, was elaborated. For the visual selection of the transformed material was used also the plasmid pDM803 that contains the reporter gene uidA (GUS).The results indicate that devices evaluated: the PIG (" Particle Inflow Gun ") and the Bio-Rad are both enough efficient to transfer foreign genes to the genome of the maize. Rev. Biol. Trop. 52(3): 787-793. Epub 2004 Dic 15.
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