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Search Results: 1 - 10 of 33435 matches for " Pierre Castro Soares "
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Aspectos epidemiológicos de la criptosporidiosis en becerros de reba?os lecheros
LIPPI ORTOLANI,ENRICO; CASTRO SOARES,PIERRE;
Parasitología latinoamericana , 2003, DOI: 10.4067/S0717-77122003000300006
Abstract: two groups of 200 dairy calves, from calving to the 60th days of life, with and without diarrhoea were selected by clinical examination of 930 calves from 30 collective calf boxes, and 13 with individual boxes, chosen at random, in the state of sao paulo, brazil. feaces smears from the calves were stained by the modified ziehl-neelsen technique. a semi-quantitative score of oocysts numbers in feaces was done. the occurrence of diarrhoea was higher during the 2nd week of life, but cryptosporidiosis was similarly found in calves from the 1st - 3rd week of life. diarrhoea and cryptosporidiosis were less frequent in older calves. calves with cryptosporidiosis excreted more oocysts and were more frequently found (38%) than asymptomatic calves (11.5%). most of these later calves were two-weeks old. calves shedding cryptosporidium oocysts were seen in 58% of the farms studied, being more prevalent in those farms that kept calves in collective boxes.
Aspectos epidemiológicos de la criptosporidiosis en becerros de reba os lecheros EPIDEMIOLOGICAL ASPECTS OF CRYPTOSPORIDIOSIS IN DAIRY CALVES
ENRICO LIPPI ORTOLANI,PIERRE CASTRO SOARES
Parasitología latinoamericana , 2003,
Abstract: Dos grupos de 200 becerros cada uno, con y sin diarrea respectivamente, fueron extraidos mediante examen clínico de un total de 930 becerros de entre 0 a 60 días de vida, seleccionados al azar en 43 establecimientos pecuarios del estado de S o Paulo, Brasil, de los cuales 30 contaban con criaderos colectivos y 13 con corrales individuales. Muestras de heces de estos animales fueron coloreadas por medio de la técnica de Ziehl-Neelsen modificada y una evaluación semi-cuantitativa del número de oocistos de Cryptosporidium en las heces fue llevada a cabo. La aparición de diarrea fue alta durante la segunda semana de vida, aunque criptosporidiosis fue igualmente detectada en becerros de 1 a 3 semanas de vida. Diarrea y criptosporidiosis fueron menos frecuentes en becerros de mayor edad. Becerros con criptosporidiosis excretan más oocistos y fueron encontrados con mayor frecuencia (68%) que becerros asintomáticos (11,5%). La mayoria de estos últimos contaba con 2 semanas de vida. Becerros cuyos deyecciones contenian oocistos fueron encontrados en 58% de los establecimientos considerados, siendo más numerosos em aquellos que mantenian los becerros en criaderos coletivos. Two groups of 200 dairy calves, from calving to the 60th days of life, with and without diarrhoea were selected by clinical examination of 930 calves from 30 collective calf boxes, and 13 with individual boxes, chosen at random, in the State of Sao Paulo, Brazil. Feaces smears from the calves were stained by the modified Ziehl-Neelsen technique. A semi-quantitative score of oocysts numbers in feaces was done. The occurrence of diarrhoea was higher during the 2nd week of life, but cryptosporidiosis was similarly found in calves from the 1st - 3rd week of life. Diarrhoea and cryptosporidiosis were less frequent in older calves. Calves with cryptosporidiosis excreted more oocysts and were more frequently found (38%) than asymptomatic calves (11.5%). Most of these later calves were two-weeks old. Calves shedding Cryptosporidium oocysts were seen in 58% of the farms studied, being more prevalent in those farms that kept calves in collective boxes.
Experimental ammonia poisoning in cattle fed extruded or prilled urea: clinical findings
Antonelli, Alexandre Coutinho;Mori, Clara Satsuki;Soares, Pierre Castro;Kitamura, Sandra Satiko;Ortolani, Enrico Lippi;
Brazilian Journal of Veterinary Research and Animal Science , 2004, DOI: 10.1590/S1413-95962004000100010
Abstract: twelve yearling girolando, rumen-fistulated steers never fed with urea before, were distributed randomly in 2 groups of 6 animals each. both groups were administered intraruminally a single dose (0.5 g/kg bw) of extruded (g1) or prilled (g2) urea to induce ammonia poisoning. the clinical picture was followed for the next 240 min. besides the classic signs the present study found 3 new additional sign: dehydration, hypothermia and ingurgitated episcleral veins. convulsion, considered the definite sign, was seen in 5 out of 6 animals from both groups. one steer (g1) had only fasciculation, while another (g2) developed typical clinical signs, but not convulsion, and recovered spontaneously without treatment. the appearance of clinical signs such as muscle tremors, sternal recumbency and convulsive episode occurred at similar times in both groups, but when analyzed altogether they took place later in g1 (p < 0.04). the 1st sign to show up was fasciculation, followed by apathy, hyperaesthesia, pushing against obstacles, muscle tremor, rumen stasis, incoordination, sternal and then lateral recumbency, mild or severe dehydration, and convulsion. higher heart rate was detected at the convulsive episodes. after the convulsions, 4 animals from each group had mild hypothermia. one steer from g2 fell down in coma and died suddenly before the beginning of the treatment. although the extruded urea postponed the clinical picture, the signs were as severe as exhibited by cattle administered prilled urea. both forms of urea offered at high dose can be harmful to cattle never fed urea.
Macrominerals balance in goats fed with spineless cactus and soybean hulls Balan o de macrominerais em caprinos alimentados com palma forrageira e casca de soja
Keyla Laura de Lira dos Santos,Adriana Guim,?ngela Maria Vieira Batista,Pierre Castro de Soares
Revista Brasileira de Saúde e Produ??o Animal , 2009,
Abstract: It were evaluated the balance of minerals in diets based on spineless cactus and different levels of soybean hulls in place of the Tifton 85 grass hay. It were used five goats, castrated, without racial pattern set, provided with permanent rumen cannula and with 40±5,16 kg of BW. The 5 x 5 Latin Square was adopted. In the experimental treatments, soybean hulls were included, in the proportions of 0%; 6,25%; 12,5%; 18,75% e 25% of the diet to replace the Tifton 85 grass hay. It was decrease in consumption of minerals and the consumption of crude protein was increased by 1444, 34 g/day, which favored the digestibility of dry matter, organic matter, crude protein and non-fibrous carbohydrate. The blood concentrations of Ca grew linearly according to the inclusion of soybean hulls in the diet and for Mg the response was quadratic and lower blood concentrations of Mg 5,02 mg/dL, with the inclusion 11,5% of soybean hulls, were checked. The urinary concentrations of K and Cl decreased linearly with the addition of soybean hulls. The inclusion of soybean hulls in the diet of goats did not provided imbalance of macrominerals: Na, Cl, P and Cl. Avaliou-se o balan o de minerais de dietas à base de palma forrageira e diferentes níveis de casca de soja em substitui o ao feno de capim-Tifton 85. Foram utilizados cinco caprinos, castrados, sem padr o racial definido, com canulas ruminais permanentes, com peso vivo médio 40±5,16 kg. Adotou-se o delineamento experimental quadrado latino 5 x 5. Nas dietas incluiu-se a casca de soja, nas propor es 0%; 6,25%; 12,5%; 18,75%, e 25% da dieta em substitui o ao feno capim-Tifton 85. Assim, houve diminui o do consumo de minerais e aumento do consumo de proteína bruta em 1444, 34 g/dia, o que favoreceu a digestibilidade da matéria seca, matéria organica, proteína bruta e carboidratos n o fibrosos. As concentra es sangüíneas de Ca aumentaram linearmente em fun o da inclus o de casca de soja na dieta e para o Mg a resposta foi quadrática e verificou-se menor concentra o sangüínea de Mg 5,02mg/dL com a inclus o de 11,5% de casca de soja. As concentra es urinárias de K e Cl diminuíram linearmente com adi o de casca de soja. A inclus o de casca de soja na dieta de caprinos n o proporcionou desbalan o dos macrominerais: Na, Ca, P e Cl.
Blood gas profile of copper-poisoned in sheep treated with ammonium tetrathiomolybdate Perfil de gases sanguíneos de ovinos intoxicados por cobre e tratados com tetratiomolibidato de am nio
Pierre Castro Soares,Marta Lizandra do Rêgo Leal,Clara Satisuki Mori,Maria Claudia Araripe Sucupira
Semina : Ciências Agrárias , 2012,
Abstract: The aim of this study was to evaluate the blood gas profile of experimentally copper-poisoned sheep (in the pre-hemolytic, hemolytic and post-hemolytic phases) that have been treated or not treated with ammonium tetrathiomolybdate. Ten lambs of the Santa Ines breed were divided into two groups: control and ATTM (treated (ammonium tetrathiomolibydate). The animals were submitted to increasing doses of copper sulfate until macroscopic hemoglobinuria was detected.All of the control animals from died within four days of hemolytic crisis, and one sheep from ATTM died during the treatment. There was no difference in blood gas parameters between experimental groups. Higher values of pCO2 were observed during the hemolytic crisis (HC) in both groups. The control group had higher mean values of hCO3 in the times HC and 2 days after hemolytic crisis (dA) when compared with the time 15 before hemolytic crises (dB). The sheep that were treated with ATTM presented lower values of hCO3 at 7dB and higher levels at the HC. The control and ATTM groups exhibited higher values of BE during the HC. Poisoning resulted in disorder in the acid-base equilibrium, characterized by metabolic alkalosis and respiratory acidosis. Treatment with ATTM was able to reverse the changes in acid-base balance in copper poisoning sheep. O objetivo desse estudo foi avaliar o perfil de gases sanguineos em ovinos experimentalmente intoxicados com cobre (fases: pré-hemoltica, hemolitica e pós-hemolitica) tratados ou n o com tetratiomolibidato de am nio (ATTM). Dez cordeiros da ra a Santa Inês foram divididos em dois grupos:controle (tratados com solu o fisiologica a 0,9%) e ATTM (tratados com 3,4 mg/kg de tetratiomolibidato de amonio). Os animais foram submetidos a doses crescentes de sulfato de cobre até a observa o de hemoglobinúria macroscópica. Todos os animais do grupo controle morreram dentro de quatro dias após observa o da hemoglobinúria, e um cordeiro do grupo ATTM morreu durante o tratamento. N o houve diferen a nas varíaveis hemogasometricas entre os grupos durante o período experimental. Maiores valores da pCO2 foram observados nos dois grupos experimentais dutante a crise hemolitica (HC). O grupo controle exibiu valores mais elevados do hCO3 na HC e dois dias após a crise hemolitica em rela o ao tempo 15 dias após a crise hemolitica. Os animais do grupo tratado com ATTM apresentaram, respectivamente, amenores e maiores valores do hCO3 sete dias antes da crise hemolitica e durante a crise hemolitica. Os dois grupos exibiram maiores valores da BE na crise hemolitica. A intoxica o
Oxidative stress profile in goats supplemented or not with selenium and vitamin E and submitted to scrotal insulation Perfil de marcadores do estresse oxidativo em caprinos suplementados ou n o com selênio e vitamina E e submetidos à insula o escrotal
Janaína Azevedo Guimar?es,Guadalupe Carvalho Xavier,Pierre Castro Soares,Alexandre Cruz Dantas
Semina : Ciências Agrárias , 2011,
Abstract: To evaluate the effect of supplementation with selenium and vitamin E in the diet of goats induced to scrotal insulation (SI) on the profile of biochemical markers of systemic oxidative metabolism 12 animals were randomly divided into two groups: G1 - no supplementation G2 - supplemented with selenium (0.1 mg / kg body weight of sodium selenite) and vitamin E (0.3 IU / kg body weight). At the end of the adjustment period of 30 days, the scrotal insulation (SI) was performed with the placement of plastic bags in the testes for 18 days. Supplementation with Se + vitamin E began 60 days before induction of SI and maintained for 42 days after the end of SI, corresponding to post-insulation (PSI). Blood samples were obtained by jugular venipuncture to obtain total blood for analysis of reduced glutathione (GSH) and plasma for analysis of thiobarbituric acid reactive substance (TBARS) and determination of ferric reducing ability of plasma (FRAP). No effect of dietary supplementation with Se + vitamin E on the profile of all the variables studied, but there was systemic oxidative action understood by a significant decrease of GSH during the SI, with a gradual increase in the PSI period, as well as lower average of FRAP observed during the SI and the highest average in the period from PSI. It was concluded that analysis of the activity of reduced glutathione (GSH) and concentrations of thiobarbituric acid reactive substances (TBARS) and ferric reducing ability of plasma (FRAP) are recommended laboratory methods to evaluate the systemic response to oxidative damage caused by thermal stress testis in goats. Para avaliar o efeito da suplementa o com selênio e vitamina E na dieta de caprinos induzidos à insula o escrotal (IE) sobre o perfil de indicadores bioquímicos do metabolismo oxidativo sistêmico foram utilizados 12 animais, os quais foram distribuídos aleatoriamente em dois grupos: G1 - sem suplementa o; G2 - suplementados com selênio (0,1 mg/Kg de peso vivo de selenito de sódio) e vitamina E (0,3 UI/Kg de peso vivo). Ao término do período de adapta o de 30 dias, realizou-se a indu o da insula o escrotal (IE) com coloca o de bolsas plásticas nos testículos por período de 18 dias. A suplementa o com Se e Vitamina E teve início 60 dias antes da indu o da IE e mantida durante 42 dias após o término da IE, correspondendo à fase pós-insula o (PIE). Amostras de sangue foram obtidas por venopun o jugular para obten o de sangue total para análise de glutationa reduzida (GSH) e plasma para análise de substancia reativa ao ácido tiobarbitúrico (TBARS) e det
Suplementa o dietética com Selênio uplementa o e Vitamina E nos parametros seminais de caprinos induzidos à insula o escrotal = Effect of diet supplementation with Selenium and Vitamin E on the seminal parameters of goats induced to scrotal insulation
Guadalupe de Carvalho Xavier,Ana Cristina Marinho Maymone,Pierre de Castro Soares,Valdemiro Amaro da Silva Junior
Acta Scientiarum : Animal Sciences , 2008,
Abstract: Avaliou-se o efeito da suplementa o alimentar com Selênio e Vitamina E sobre os parametros seminais de caprinos submetidos à insula o escrotal (IE). Utilizaramse 12 animais distribuídos, aleatoriamente, em grupos-controle (G1) e Selênio e Vitamina E (G2). Dois meses após o início da suplementa o alimentar nos animais do G2, realizou-seIE durante 18 dias. Após este período (IE), a suplementa o foi mantida por mais 42 dias (fase pós-insula o escrotal, PIE). Para análise seminal, foram efetuadas seis colheitas de sêmen (antes, durante e após a IE). N o foi observado efeito da suplementa o com Selênio e Vitamina E nas características quanti-qualitativas do sêmen. Com exce o do volume seminal, foi observada diferen a significativa (p < 0,05) entre os dias de colheita para ambosos grupos, com redu o de motilidade, vigor, concentra o espermática, integridade do acrossoma e de DNA. Decorridos 42 dias da IE, observou-se normalidade de motilidade, vigor espermático, integridade de acrossoma e de DNA em um animal de cada grupo.Conclui-se que a temperatura elevada nos testículos de caprinos submetidos à IE altera os parametros seminais; a suplementa o com Selênio e Vitamina E, nas concentra es de 0,1 e 0,3 mg kg-1 PV-1, respectivamente, n o é suficiente para minimizar os efeitos deletérios da insula o escrotal induzida. This study evaluated the effect of Selenium and Vitamin E supplementation on the semen parameters of goats induced to scrotal insulation (SI). Twelve animals were used, distributed randomly in twogroups (G1 = Control; G2 = Selenium and Vitamin E). Two months after the start of diet supplementation on G2 animals, it was realized SI was induced during 18 days. After this period (SI), supplementation was maintained during 42 days, corresponding to the postinsulationphase (PSI). For semen analysis, six semen collections were performed (before, during and after SI). There was no effect of Selenium and Vitamin E supplementation on the quanti-qualitative characteristics of the semen. With the exception of semen volume, a significant effect (p < 0.05) was observed between collection days for both groups (G1 and G2), with reduction in motility, vigor, sperm concentration, acrosome and DNA integrity. After 42 days of SI, normal values were observed for motility and sperm vigor, acrosomeand DNA integrity in one animal per group. It can be concluded that the high temperature of the testes of goats subjected to SI alters semen parameters; Selenium and Vitamin E supplementation on goats, in a concentration of 0.1 and 0.3 mg kg-1 BW-1, respectively, wa
Teores séricos e hepáticos de cobre, ferro, molibdênio e zinco em ovinos e caprinos no estado de Pernambuco
Marques, Allan Vieira dos Santos;Soares, Pierre Castro;Riet-Correa, Franklin;Mota, Iagmar Oliveira da;Silva, Tércya Lucid de Araújo;Borba Neto, álvaro Veloso;Soares, Filipe Aureliano Pedrosa;Alencar, Sylvana Pontual de;
Pesquisa Veterinária Brasileira , 2011, DOI: 10.1590/S0100-736X2011000500006
Abstract: the aim of this research was to determine serum and liver concentrations of cu, mo, fe and zn in sheep and goats slaughtered in the semiarid region of the state of pernambuco, northeastern brazil, during the rainy and dry seasons, and to establish if cu deficiency which occurs in the region is primary or secondary to high levels of mo and/or fe. serum and liver samples from 141 goats and 141 sheep were submitted to wet digestion in nitric-perchloric acid and analyzed by coupled plasma atomic absorption spectrophotometry (icpoes). the mean serum concentrations of cu were 9.85±2.71μmol/l in sheep and 11.37±2.57μmol/l in goats. mean cu liver concentrations were 158.45±83.05mg/kg in sheep and 152.46±79.58mg/ kg in goats. mean serum concentrations of fe were 35.58±14.89μmol/l in sheep and 25.06±8.10μmol/l in goats. mean liver concentrations of fe were 156.10±55.99mg/kg in sheep and 210.53±121.99mg/kg in goats. mean serum concentrations of mo were 0.28±0.11μmol/l in goats and 0.31±0.16μmol/l in sheep. mean liver concentrations of mo were 6.53±4.13mg/kg in goats and 8.10±4.01mg/kg in sheep. mean serum concentrations of zn were 11.9±6.07μmol/l in sheep and 11.79±7.42μmol/l in goats. mean liver concentrations of zn were 126.43±51.50mg/kg in sheep and 132.91±55.28mg/kg in goats. based on cu serum and liver concentrations and on the sporadic occurrence of enzootic ataxia, cu supplementation is recommended for grazing sheep and goats during the dry and the rainy seasons. considering that serum and hepatic concentrations of fe and mo are within normal ranges or are marginal, it is suggested that the low cu concentrations are not related with high fe or mo ingestion. non significant differences were observed between serum and liver concentrations of cu and mo, and between serum concentrations of fe in samples collected during the rainy season and the dry season. liver concentrations of fe and serum and liver concentrations of zn were significantly higher during the rainy season.
Avalia??o laboratorial do uso de solu??o salina hipert?nica e isot?nica e de furosemida no tratamento da intoxica??o por am?nia em bovinos
Kitamura, Sandra Satiko;Antonelli, Alexandre Coutinho;Maruta, Celso Akio;Sucupira, Maria Claudia Araripe;Mori, Clara Satsuki;Yonezawa, Letícia Andreza;Michima, Lilian Emy dos Santos;Soares, Pierre Castro;Ortolani, Enrico Lippi;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010005000131
Abstract: to test the efficiency of some treatments for ammonia poisoning in cattle, 25 steers were used. ammonium chloride solution was infused intravenously (iv) in each steer until the onset of convulsive episode. thereafter, the animals were distributed in one of the five different groups, as follows: 1) control: infusion (iv) of 300ml isotonic saline solution (iss) throughout the following 4h; 2) infusion (iv) of iss 30ml kg-1 bw throughout the following 4h and administration of 4l water (w) through stomach tube; 3) the same as group 2, plus a single dose (iv) 2g furosemide kg-1 bw (f); 4) injection of 5ml kg-1 bw hypertonic saline solution (hss) (7.2%) (iv) throughout the first 30min, followed by 20ml kg-1 bw of iss and 4l w.; 5) the same as group 4 and a single dose of f. for the next 4h after the convulsion, plasma concentration of ammonia and glucose, serum urea, creatinine, sodium and potassium, urine total volume and specific gravity, and urinary excretion of ammonium and urea were followed. at the convulsion, the ammonia blood levels were high and similar among the groups, but at the 120th min the animals treated with hss+iss+w (groups 4 and 5) had lower values than the control. furosemide (groups 3 and 5) did not prevent an increase in the total excretion of urine. the therapy with hss+iss+w still increased the urinary volume and the total urinary excretion of urea and ammonium during the critical 1st h of treatment, while the use of iss+w had intermediate results. the efficiency of the treatment with hss+iss+w or iss+w was better than the control group. although with lower efficiency as seen in the treatment with hss+iss+w, the iss+w caused an improvement in the clinical picture and at the end of the experiment generated an adequate ammonia detoxification.
Proteinograma e teores de cobre, ferro e zinco no soro sanguíneo de ovelhas da ra?a Santa Inês com mastite experimental por Staphylococcus aureus
Costa, Nivaldo de Azevêdo;Sim?o, Luis Carlos Valeriano;Santos, Rogério Adriano dos;Afonso, José Augusto Bastos;Fagliari, José Jurandir;Cardoso, Elyzabeth da Cruz;Soares, Pierre Castro;Mendon?a, Carla Lopes de;
Pesquisa Veterinária Brasileira , 2010, DOI: 10.1590/S0100-736X2010000500011
Abstract: the aim of the present study was to evaluate the effect of staphylococcus aureus experimentally induced mastitis on proteinogram and serum concentrations of cupper, iron and zinc levels of santa ines primiparous ewes . the right mammary gland of ten healthy ewes was inoculated with 1,0x104 ufc/ml of s. aureus. clinical examination and determination of serum concentrations of proteins by electrophoresis in polyacrylamide gel (sds-page), cupper, iron and zinc, as well plasma level of fibrinogen were measured before the inoculation (control) and 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h, 108h, 120h, 132h, 168h, 180h, 288h and 336h after bacteria inoculation. all animals experimentally infected presented clinical mastitis and subsequent loss of mammary gland function. the electrophoretogram allowed the identification of 23 proteins with molecular weights (mw) ranged from 26.000 to 185.000 daltons (da) including acute-phase proteins, igg and iga. a significant increase (p<0,05) in haptoglobin, ceruloplasmin, igg and iga concentrations was observed. antitrypsin and acid glicoprotein concentrations did not alter. the levels of iron and zinc decreased and the cupper concentration increased . a positive correlation between plasma fibrinogen and serum ceruloplasmin (r=0.74), haptoglobin (r=0.62) and iga (r=0.62) was also identified. results showed the importance of ceruloplasmin and haptoglobin as acute-phase proteins in ewes with intramammary infections and confirms fibrinogen as an inflammatory marker because its high correlation with specific proteins. the alterations in the serum levels of cu, fe and zn suggest the action of inflammatory mediators triggered by s. aureus.
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