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Quantitative estimation of silybin in Silybum marianum mother tincture using high performance liquid chromatography
Hamrapurkar Purnima,Thakurdesai Leena,Phale Mitesh
International Journal of Green Pharmacy , 2010,
Abstract: Silybin is a characterising compound present in the plant Silybum marianum, used as an hepatoprotective. A simple, accurate and sensitive HPLC method was developed for estimation of silybin. The chromatographic separation was carried out on RP C18 (Waters) column (250 × 4.6 mm, 10 μm) using mobile phase, phosphate buffer: water (65:35 % v/v) pumped at a flow rate of 1 ml/min. The eluent was monitored at 288 nm. The method is specific and linear over the range of 10-200 ng/ml. The method was statistically validated for precision, accuracy, robustness and recovery. The HPLC method can be applied for identification and on of silybin in herbal extracts of Silybum marianum.
Cloning, Purification, Crystallization and Preliminary X-Ray Diffraction Studies of Periplasmic Glucose Binding Protein of Pseudomonas putida CSV86  [PDF]
Suman Pandey, Arnab Modak, Prashant S. Phale, Prasenjit Bhaumik
Advances in Bioscience and Biotechnology (ABB) , 2015, DOI: 10.4236/abb.2015.63016
Abstract: Biochemical data and genomic analysis indicate the involvement of a putative ABC transporter for glucose transport in Pseudomonas putida CSV86. The periplasmic solute binding proteins are known to confer substrate specificity to the ABC transporters by binding specifically to the substrate and transferring them to their cognate inner membrane transport assembly. Periplasmic glucose binding protein from Pseudomonas putida CSV86 (ppGBP) was found to be glucose specific. The gene encoding ppGBP was cloned. Recombinant ppGBP was overexpressed and purified to homogeneity. The purified recombinant protein showed glucose binding activity of 752 pmol/mg of protein and was crystallized as a complex with glucose. The crystal diffracted to 1.7 Å resolution using home X-ray source. Preliminary analysis of diffraction data showed that the crystals belonged to space group P21212 with unit-cell parameters a = 102.56, b = 119.2, c = 66.65 Å and α = β = γ = 90°. Matthews coefficient calculation showed the presence of two molecules in the asymmetric unit with solvent content of 45.7%.
Underdiagnosed Distal Colonic Volvuli on MDCT Imaging  [PDF]
Mitesh Kumar, Siddharth Saravanan Vijayakumar, Bhawna Dev
Surgical Science (SS) , 2014, DOI: 10.4236/ss.2014.53020
Abstract:

Volvuli affecting the distal colon, specifically the splenic flexure onwards is underdiagnosed pathologies with non-specific clinical features. Delay in diagnosing these pathologies can lead to significant morbidity and mortality in terms of ischemia and perforation of bowel. Multi detector computed tomography (MDCT) with intravenous contrast is an accurate and reliable imaging modality allowing prompt diagnosis and management. We present an overview of the radiological features found in acute presentations of different types of colonic volvuli.

Concentration of Soluble Condensed Tannins and Neutral Detergent Fibre-bound Tannins in Fodder Trees and Forage Crops in Botswana
O. Phale,O.R. Madibela
Journal of Biological Sciences , 2006,
Abstract: This study was conducted to determine soluble and fibre-bound condensed tannins in fodder trees and forages used in ruminant feeding in Botswana. Samples of Acacia nilotica and A. robusta were obtained from paddocks at Content Farm while Lablab purpureus (Lablab) was obtained from dried hay bales and Ipomoea batatas (sweet potato) was sourced from Department of Agricultural Research. Acacia and sweet potato vine samples were dried under shade before been ground and analysed for soluble tannins and fibre-bound tannins. It was found that amounts of soluble and fibre-bound tannins were different between plant species. There was a highly significant (p<0.001) plant effect on soluble tannins. A. nilotica had the highest levels while Ipomoea batatas had the least amounts. The fibre-bound tannins was significantly different (p<0.01) between plant species. Once again A. nilotica had high levels while sweet potato had the least. However A. robusta and Lablab had similar (p>0.05) amounts of fibre-bound tannins. The level of soluble tannins in acacia species may interfere with animal performance since they exceed 50 g kg-1. However, goats may be able to utilise these plants as no sign of toxicity was found in goats consuming 10-23 g kg-1 of tannins-rich leaves in Israel.
Evaluation of Documentation Practices of Sexual Assault Nurse Examiners
Minshall, Lisa,Patel, Mitesh
Western Journal of Emergency Medicine : Integrating Emergency Care with Population Health , 2001,
Abstract:
The Information Content of Undisclosed Limit Orders Around Broker Anonymity
Andrew Lepone,Mitesh Mistry
Australasian Accounting Business and Finance Journal , 2011,
Abstract: This paper examines the impact of the submission of undisclosed limit ordersand their short‐term information content compared to similar disclosed orders.We also examine whether the abolishment of broker identifiers from tradingscreens on the Australian Securities Exchange affects the short‐term informationcontent of various order types. Results indicate that aggressively submittedundisclosed orders, compared to similar disclosed‐limit orders, lead tosignificantly higher short‐term price movements. The removal of brokeridentifiers does not provide consistent evidence of any changes in the short‐terminformation content of large dollar volume orders. This suggests that disclosedorders provide more information to the market than do broker identifiers.
Proving Skipping Refinement with ACL2s
Mitesh Jain,Panagiotis Manolios
Computer Science , 2015, DOI: 10.4204/EPTCS.192.9
Abstract: We describe three case studies illustrating the use of ACL2s to prove the correctness of optimized reactive systems using skipping refinement. Reasoning about reactive systems using refinement involves defining an abstract, high-level specification system and a concrete, low-level system. Next, one shows that the behaviors of the implementation system are allowed by the specification system. Skipping refinement allows us to reason about implementation systems that can "skip" specification states due to optimizations that allow the implementation system to take several specification steps at once. Skipping refinement also allows implementation systems to, i.e., to take several steps before completing a specification step. We show how ACL2s can be used to prove skipping refinement theorems by modeling and proving the correctness of three systems: a JVM-inspired stack machine, a simple memory controller, and a scalar to vector compiler transformation.
Skipping Refinement
Mitesh Jain,Panagiotis Manolios
Computer Science , 2015,
Abstract: We introduce skipping refinement, a new notion of correctness for reasoning about optimized reactive systems. Reasoning about reactive systems using refinement involves defining an abstract, high-level specification system and a concrete, low-level implementation system. One then shows that every behavior allowed by the implementation is also allowed by the specification. Due to the difference in abstraction levels, it is often the case that the implementation requires many steps to match one step of the specification, hence, it is quite useful for refinement to directly account for stuttering. Some optimized implementations, however, can actually take multiple specification steps at once. For example, a memory controller can buffer the commands to the memory and at a later time simultaneously update multiple memory locations, thereby skipping several observable states of the abstract specification, which only updates one memory location at a time. We introduce skipping simulation refinement and provide a sound and complete characterization consisting of "local" proof rules that are amenable to mechanization and automated verification. We present case studies that highlight the applicability of skipping refinement: a JVM-inspired stack machine, a simple memory controller and a scalar to vector compiler transformation. Our experimental results demonstrate that current model-checking and automated theorem proving tools have difficultly automatically analyzing these systems using existing notions of correctness, but they can analyze the systems if we use skipping refinement.
Characterization & Simulation Of Class AB Current Conveyor Second Generation (CCII) Based On Current Mirrors
Dhaval P. Patel,Mitesh Patel
Golden Research Thoughts , 2013, DOI: 10.9780/22315063
Abstract: Research in analog integrated circuits has recently gone in the direction of low voltage (LV) lowpower (LP) design especially in the environment of portable systems. In this area traditional voltage-mode techniques are going to be substituted by the current-mode approach which has the advantage to overcome the gain-bandwidth product limitation typical of operational amplifier. Then they do not require high voltage gains and have good performance in terms of speed bandwidth and accuracy. Inside the current-mode architectures the current-conveyor (CCII) can be considered the basic circuit block because all the active devices can be made of a suitable connection of one or two CCIIs. Recent advances in integrated circuit technology have also highlighted the usefulness of CCII solutions in a large number of signal processing applications. This project describes different topologies of CCIIs. All topologies have been simulated in different CMOS process technologies using Eldo Spice tool and layout is made using Mentor Graphics Back End tools like IC Station and DA-IC. Different characteristics such as gain, bandwidth, terminal impedances, slew rate, dynamic range, input-output current characteristic and offset are measured and tabulated for all theCCII topologies.
An Analysis of Road Traffic Collisions on Roads of Sabarkantha District of Gujarat
Patel Mitesh K, Kartha GP
National Journal of Community Medicine , 2010,
Abstract: This study on road traffic accidents in Sabarkantha district conducted from January to December 2002 among National and State Highways and town/ village roads examines the pertinent epidemiological variables thereof. Of the 512 event of road traffic accidents 81.6% were non-fatal and 18.4% were fatal with domination of pedestrian accidents (37.8%). The highest events (40%) took place on National highway followed by State highways (31.4%) and town/village roads (28.5%) and events like a€ vehicle head ona€ , a€ vehicle hit from backa€ , a€ vehicle hit from sidea€ and a€ run off the roada€ were higher on National highway. The study point to the need for proper traffic management at all levels starting from the village and town roads to the State and National highways to control the avoidable morbidity and mortality associated with road traffic accidents.
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