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Sobrevivência saprofítica de Alternaria brassicicola e manejo de restos foliares de brócolos
Peruch, Luiz Augusto Martins;Michereff, Sami Jorge;
Ciência Rural , 2007, DOI: 10.1590/S0103-84782007000100003
Abstract: the saprophytic survival of the fungus alternaria brassicicola was investigated in leaf debris of broccoli (brassica oleracea var. italica), at different depths of soil incorporation, periods of the year, and systems of soil management. infected leaf debris were distributed in field plots, at the soil surface and at depths of 5 and 10cm. periodically, the debris in the bags were collected and conidia concentrations were quantified for longevity of spore production and extinction rate. higher spore production longevity was observed on leaf debris in the period with lower temperatures, higher air relative humidity and lower rainfall. the lowest spore production occurred when leaf debris incorporation was made at 10cm depth. in relation to soil management systems, when the inoculum source was deposited at the soil surface no differences in the rate of spore production were observed, but at depths of 5 and 10cm, the rate was significantly higher in the soil under conventional management. the management of alternaria black spot on broccoli at the area under study can be accomplished by incorporating infected leaf debris in the soil, at least 10cm depth, for no less than a 60-day interval in subsequent brassica plantings.
Fungicides and silicate clay on the control of antracnosis in yellow passionfruit Fungicidas e argila silicatada no controle da antracnose do maracujá amarelo
André Martins Medeiros,Luiz Augusto Martins Peruch
Semina : Ciências Agrárias , 2012,
Abstract: The anthracnose, caused by Glomerella cingulata (anamorf Colletotrichum gloeosporioides), is the most important fungal disease for aerial parts of yellow passionfruit. However, few studies about anthracnose control in field conditions have been published. The objective of this research was to evaluate silicate clay and fungicides for anthracnose control. Treatments evaluated were: tebuconazole (20g i.a./100L), copper oxychloride (180g i.a./100L), silicate clay (15g /L) and control (untreated plants). The sanity of the plants was evaluated through area under disease progress curve (AUCPD), final severity (SEVF) and disease progress curves. Experimental design was completely randomized with five repetitions. Tebuconazole and silicate clay proportioned significant reduction for AUCPD and SEVF compared to control. Copper oxychloride reduced only SEVF. Treatment with tebuconazole reduced AUCPD and SEVF in 75% and 71%, and silicate clay in 82%, respectively. Copper oxychloride reduced SEVF in 76%. The disease progress curves confirmed results and showed lower growth of anthracnose for tebuconazole and silicate clay. Silicate clay and tebuconazole are efficient to control anthracnose in passionfruit. A antracnose, causada por Glomerella cingulata (anamorfo Colletotrichum gloesporioides), é a principal doen a fúngica de parte aérea do maracujazeiro amarelo. Todavia, poucos estudos de controle da doen a a campo têm sido publicados, motivo pelo qual é importante avaliar diferentes fungicidas e substancias alternativas para o manejo da antracnose. Neste trabalho foram avaliados os seguintes tratamentos: tebuconazole (20g i.a./100L), oxicloreto de cobre (180g i.a./100L), argila silicatada (15g /L) e testemunha. A partir da avalia o da severidade da doen a, determinou-se a área abaixo da curva de progresso da doen a (AACPD), severidade final (SEVF) e as curvas de progresso da doen a. O delineamento adotado foi inteiramente casualizado com cinco repeti es. Tebuconazole e argila silicatada proporcionaram os melhores resultados no controle da doen a pela redu o significativa da AACPD e SEVF em rela o à testemunha. Oxicloreto de cobre diferiu da testemunha apenas para SEVF. Tratamento com tebuconazole reduziu AACPD e SEVF em 75% e 71%, e argila silicatada em 82% as variáveis, respectivamente. Oxicloreto de cobre reduziu SEVF em 76%. As curvas de progresso da doen a corroboram os resultados pelo menor crescimento da antracnose para tebuconazole e da argila silicatada. O Tebuconazole e a argila silicatada s o eficientes no controle da antracnose do maracujazeiro.
Rela??o entre doses de calda bordalesa e de fosfito potássico na intensidade do míldio e na produtividade da videira cv. 'Goethe'
Peruch, Luiz Augusto Martins;Bruna, Emílio Della;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000900001
Abstract: the downy mildew, considered the most important disease of grape, causes great damage to the culture in the south of brazil. news aspects of control, like new substances, should still be studied to reduce disease intensity. the objective of this research was to evaluate efficiency of bordeaux mixture and phosphites on control of downy mildew and productivity of grape cv. 'goethe' in the south coastal of santa catarina, brazil. the concentrations tested of 0.0; 0.1; 0.2; 0.4 e 0.8% of the fungicide and the foliage fertilizer were evaluated in a dbc factorial scheme with three repetitions. the aacpd, bunch diseased incidence (incidence) and productivity were submitted to regression analyses and f test. differences were observed for aacpd and incidence for concentrations, but not to different treatments and interactions between treatments and concentrations. the doses of 0.4% bordeaux mixture and 0.3% phosphite reduced the aacpd in 98 and 94% and incidence in 46 and 76%, respectively. phytotoxycity symptoms were observed in 0.8% and 0.4% doses of bordeaux mixture and phosphites, respectively. no differences were verified for productivity. the dosis of 0.4% of bordeaux mixture and 0.3% of phosphites resulted in control of downy mildew of grape.
Reduction of Sulfur Dioxide on Carbons Catalyzed by Salts
Eduardo Humeres,Maria da Gloria B. Peruch,Regina F.P.M. Moreira,Wido Schreiner
International Journal of Molecular Sciences , 2005, DOI: 10.3390/i6010130
Abstract: The reduction of SO2 on different carbons in the presence of the nitrates and sulfides of sodium, potassium and calcium and potassium polysulfides was studied. The presence of salts increased the initial rate 2-5 fold for all of them and did not change the product distribution. The catalysis was not determined by the cation and there was no difference in the catalytic reactivity between nitrates and sulfides. The sulfur content of the activated carbon increased during the reaction on account of the stable reactive intermediates in the reduction of SO2. In the presence of NaNO3 or Na2S, the amount of sulfur incorporated was in the molar ratio Na:S = 3 ± 0.3, and the XPS spectra of the residual carbon showed an increase of ca. 9% of the non-oxidized form of sulfur in the intermediates. In the absence of salt, it is proposed that after the adsorption of SO2 on the carbon, a 1,3,2-dioxathiolane or 1,2-oxathietene 2-oxide are formed and that decompose to produce CO2 and atomic sulfur. The non-oxidized sulfur intermediate would be an episulfide 3, formed from the reaction of the atomic sulfur with the nearest double bond and followed by consecutive reactions of insertion of atomic sulfur to form a trisulfide. Extrusion of S2 from the trisulfide would regenerate the episulfide, establishing a sulfidedisulfide-trisulfide equilibrium that worked as a capture-release cycle of sulfur. In the presence of salt, the results are consistent with the assumption that the episulfide 3 reacts with the corresponding sulfide anion to form a disulfide anion, which upon reaction with atomic sulfur forms a trisulfide anion that decomposes releasing diatomic sulfur S2, transporting the sulfur and generating a thiolate that is part of the catalytic cycle.
Levantamento da intensidade da alternariose e da podrid?o negra em cultivos organicos de brássicas em Pernambuco e Santa Catarina
Peruch, Luiz A M;Michereff, Sami J;Araújo, Issac B;
Horticultura Brasileira , 2006, DOI: 10.1590/S0102-05362006000400014
Abstract: the objective of this research was to evaluate the intensity of alternaria black spot, caused by alternaria brassicicola and/or alternaria brassicae, and black rot, caused by xanthomonas campestris pv. campestris in organic cultivation of brassicas in pernambuco and santa catarina. the survey was carried aut from november 2001 to february 2002, in 103 fields under organic farming systems with different brassicas. high prevalence of the diseases was registered in both states, except on chinese cabbage in santa catarina. prevalence of alternaria black spot was 100% on broccoli fields in pernambuco, as well as on cauliflower in both states, while the black rot reached that level on broccoli and cauliflower fields in santa catarina. on the average of the different brassica species, the diseases were more prevalent in pernambuco than in santa catarina. however, when the severity averages of each disease were considered, no significant differences were observed between the two states, although the climatic conditions were highly different. the alternaria black spot severity varied among the brassica species in pernambuco, being lower on kale. in santa catarina no significant differences were observed among the brassicas species. in relation to the black rot, only in santa catarina was there a difference in the disease severity, with the lowest level on chinese cabbage. no significant correlations were observed either between severity levels of alternaria black spot and black rot, neither between disease severity and total number of plants or plant age.
Adsorption of textile dyes on alumina. equilibrium studies and contact time effects
MOREIRA, R.F.P.M.;PERUCH, M.G.;KUHNEN, N. C.;
Brazilian Journal of Chemical Engineering , 1998, DOI: 10.1590/S0104-66321998000100002
Abstract: the use of nonconventional adsorbents, particularly those that can be easily regenerated, to replace activated carbon in the removal of color from dye wastewaters has been recently proposed. this work shows a thermodynamic and kinetic study of the adsorption of reactive dyes (yellow monochlorotriazine and yellow dichlorotriazine), in liquid phase, on commercial alumina. the basic thermodynamic data were obtained using the static method, with a thermostatic bath at four different temperatures (30, 40, 50 and 60oc) and different ph values. the kinetic data were obtained by adding a known quantity of adsorbent to a dye solution at a constant temperature and under controlled stirring conditions. it was possible to draw the uptake curves, using the effects of the stirring on the adsorption rate. the intraparticle effective diffusivity was estimated using the film and pore diffusion model. the results were compared with the data obtained using a commercial activated carbon.
Adsorption of textile dyes on alumina. equilibrium studies and contact time effects
MOREIRA R.F.P.M.,PERUCH M.G.,KUHNEN N. C.
Brazilian Journal of Chemical Engineering , 1998,
Abstract: The use of nonconventional adsorbents, particularly those that can be easily regenerated, to replace activated carbon in the removal of color from dye wastewaters has been recently proposed. This work shows a thermodynamic and kinetic study of the adsorption of reactive dyes (yellow monochlorotriazine and yellow dichlorotriazine), in liquid phase, on commercial alumina. The basic thermodynamic data were obtained using the static method, with a thermostatic bath at four different temperatures (30, 40, 50 and 60oC) and different pH values. The kinetic data were obtained by adding a known quantity of adsorbent to a dye solution at a constant temperature and under controlled stirring conditions. It was possible to draw the uptake curves, using the effects of the stirring on the adsorption rate. The intraparticle effective diffusivity was estimated using the film and pore diffusion model. The results were compared with the data obtained using a commercial activated carbon.
Revestimento ativo de amido na conserva??o pós-colheita de pera Williams minimamente processada
Botrel, Diego Alvarenga;Soares, Nilda de Fátima Ferreira;Camilloto, Geany Peruch;Fernandes, Regiane Victória de Barros;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010000800023
Abstract: pear is one of the most consumed temperate fruits in brazil; however their conservation is limited due to browning when it suffers injuries or physical treatments. the edibles coating interact with the food positively extending its shelf life. this research aimed to evaluate the action of starch edible coating incorporated with calcium lactate and l-cysteine on enzymatic browning inhibition, on psychrotrophs and enterobacteriaceae growing reduction and on firmness maintenance. the sliced pears were coated with starch edible coating incorporated with l-cysteine and calcium lactate, except control, without coating (c). the treatment were: only coating (t1); 2,0% calcium lactate and 1,0% l-cysteine (t2); 2,0% calcium lactate and 1,5% l-cysteine (t3). the samples were taken at 0, 2, 4 and 6 days. pears were keeped under refrigeration (7°c±2°c). pears submitted to treatments t2 and t3 show significantly more firms (p<0,05) compared to treatments c and t1. cysteine action over enzymatic browning inhibition was observed in treatments t2 and t3 which do not differed significantly each other (p≥0,05) to ?e values however these were significantly lower than control (c). at time 6, decimal reduction on psychrotrophs counting reached 3,03 and 2,43 to t3 e t2 compared to control. enterobacteriaceae counting showed similar behavior where the reduction values were 3,16 and 3,05 to t2 e t3 compared to control. it was verified that using the studied edible coating on fresh cut pear can extend its shelf life.
Rubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties
Karine Berthelot, Sophie Lecomte, Yannick Estevez, Bénédicte Coulary-Salin, Ahmed Bentaleb, Christophe Cullin, Alain Deffieux, Frédéric Peruch
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0048065
Abstract: REF (Hevb1) and SRPP (Hevb3) are two major components of Hevea brasiliensis latex, well known for their allergenic properties. They are obviously taking part in the biosynthesis of natural rubber, but their exact function is still unclear. They could be involved in defense/stress mechanisms after tapping or directly acting on the isoprenoid biosynthetic pathway. The structure of these two proteins is still not described. In this work, it was discovered that REF has amyloid properties, contrary to SRPP. We investigated their structure by CD, TEM, ATR-FTIR and WAXS and neatly showed the presence of β-sheet organized aggregates for REF, whereas SRPP mainly fold as a helical protein. Both proteins are highly hydrophobic but differ in their interaction with lipid monolayers used to mimic the monomembrane surrounding the rubber particles. Ellipsometry experiments showed that REF seems to penetrate deeply into the monolayer and SRPP only binds to the lipid surface. These results could therefore clarify the role of these two paralogous proteins in latex production, either in the coagulation of natural rubber or in stress-related responses. To our knowledge, this is the first report of an amyloid formed from a plant protein. This suggests also the presence of functional amyloid in the plant kingdom.
Increased presevation of sliced mozzarella cheese by antimibrobial sachet incorporated with allyl isothiocyanate
Pires, Ana Clarissa dos Santos;Soares, Nilda de Fátima Ferreira;Andrade, Nélio José de;Silva, Luis Henrique Mendes da;Camilloto, Geany Peruch;Bernardes, Patrícia Campos;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000400036
Abstract: there is an increasing tendency to add natural antimicrobials of plant origin into food. the objective of this work was to develop a microbial sachet incorporated with allyl isothiocyanate (ait), a volatile compound of plant origin, and to test its efficiency against growth of yeasts and molds, staphylococcus sp. and psychrotrophic bacteria on sliced mozzarella cheese. another objective was to quantify the concentration of ait in the headspace of cheese packaging. a reduction of 3.6 log cycles was observed in yeasts and molds counts in the mozzarella packed with the antimicrobial sachet over 15-day storage time. the sachet also showed an antibacterial effect on staphylococcus sp., reducing 2.4 log cycles after 12-day storage. psychrotrophic bacteria species were the most resistant to the antimicrobial action. the highest concentration of ait (0.08μg.ml-1) inside the active packaging system was observed at the 6-day of storage at 12 oc ± 2 oc. at the end of the storage time, ait concentration decreased to only 10% of the initial concentration. active packaging containing antimicrobial sachet has a potential use for sliced mozzarella, with molds and yeasts being the most sensitive to the antimicrobial effects.
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