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“Good Vibrations”: The Social Networks of Optimists and Alter-Optimists  [PDF]
Miguel Pereira Lopes
Social Networking (SN) , 2012, DOI: 10.4236/sn.2012.11001
Abstract: This study empirically tested if the personality trait of optimism and the interpersonal capability to generate optimism in one’s network nodes (i.e., alter-optimism) influences the social relationship patterns. The results provide evidence that optimism trait is independent from the way social networks of personal-issue sharing, advice-seeking, problem-solving, and innovation, are structured. In contrary, the alter-optimism capability does provide a good explanation of one’s social network position. Implications of these findings are discussed at the end.
A time to look within curricula—Nursing students’ perception on sexuality and gender issues  [PDF]
Maryann Washington, Edwina Pereira
Open Journal of Nursing (OJN) , 2012, DOI: 10.4236/ojn.2012.22010
Abstract: Nursing students could be viewed as dynamic change agents given the knowledge and skills they would be equipped with. Amongst all areas to be addressed in their clientele of the hospital or community setting, one key area that a nurse could focus on is sexuality. This however is often neglected for simple reasons such as lack of knowledge, embarrassment, fear of intrusion on privacy, or it is perceived as an un-important concern of the patient. Focus groups discussions combined with self report were conducted on 84 nursing students selected by the faculty from three institutions of nursing to assess their perceptions related to sexuality and gender issues, and thus extrapolate on their learning needs within the curriculum. Student expressed discomfort in caring for patients who had sexual expressions and relationships different from the accepted cultural norm; perceived sexuality primarily as heterosexual relationship; helplessness when faced with sexual harassment in the health care field and gaps in their present curricula in relation to contextualization of sexuality and gender issues.
A Dialectical Approach to Positive Organizational Studies  [PDF]
Miguel Pereira Lopes
American Journal of Industrial and Business Management (AJIBM) , 2013, DOI: 10.4236/ajibm.2013.32024
Abstract:

Over the last decade, an academic movement emerged towards the study of positive phenomena in management and organization studies. Since then, two different scientific research streams have emerged in line with this positive approach to management: 1) positive organizational scholarship (POS), which proposes a trait approach view of positive virtues and strengths and sees the environment as a moderator variable which facilitates or not the exhibition of corresponding positive behaviours; and positive organizational behaviour (POB), which defends a state-like perspective of positive characteristics, thus putting a strong emphasis on situational factors as determinants of positive behaviour, leaving a marginal role to positive psychological traits. As a critical comparison between these two different research streams is yet to be done, in this paper I propose a dialectical approach to study positivity in organizations and contrast these two different ontological approaches to positivity in organizations. I presented arguments to demonstrate that each of these approaches alone constitute quite a limited proposal in that each of them seems to misleadingly assume that: traits cannot be changed; they show incongruence between assumptions and purpose and; they constitute biased viewpoints. A dialectical approach makes possible to overcome these shortcomings by assuming both personality characteristics and environmental features relate each other in an intertwined complex way to produce positive behaviour in organizations. I finally present some practical implications that a dialectical approach would have to organizations and managers.

Modulation of biological activities produced by an endophytic fungus under different culture conditions  [PDF]
Henrique Pereira Ramos, Suraia Said
Advances in Bioscience and Biotechnology (ABB) , 2011, DOI: 10.4236/abb.2011.26065
Abstract: The effect of culture conditions on the production of bioactive secondary metabolites by the endophytic fungus Arthrinium State of Apiospora Montagnei Sacc. was investigated. Culture broths were partitioned with ethyl acetate and the resulting extracts were evaluated for antibacterial, antifungal, cytotoxic, and antiparasitic activities. The highest levels of activities were arisen from cultures cultivated at 30C in modified Czapek medium. The best antimicrobial activity against Escherichia coli and Pseudomonas aeruginosa (MIC 90 μg/ml), and against Aspergillus fumigatus (MIC 130 μg/ml) were found in extracts from cultures grown in medium containing 3.1% (w/v) sucrose and 0.1% (w/v) sodium nitrate at pH 4.0 after 9 days of incubation. Cultures grown in medium without modification also showed antiparasitic and antitumoral activities after 9 days at pH 4.0, antifungal activity against Candida albicans at pH 4.0 - 7.0 (MIC 140 μg/ml), but against A. fumigatus just after 27 days of incubation. A specific bioactive compound may have its production improved if the culture conditions parameters that affect or influence the production were known, which in turn makes the purification process easier.
Calling for Authentic Leadership: The Moderator Role of Calling on the Relationship between Authentic Leadership and Work Engagement  [PDF]
Victor Seco, Miguel Pereira Lopes
Open Journal of Leadership (OJL) , 2013, DOI: 10.4236/ojl.2013.24015
Abstract:
Authentic Leadership (AL) literature supports the existence of a positive relationship between perceived AL, follower work engagement, and positive attitudes like calling. Our research doesn’t confirm that relationship and fosters the possibility of AL influence could not be felt by employees. It was also expected, theoretically, a positive effect of Calling as well as with trust. We have found a significant negative effect on the relationship between AL and calling. But our findings brought some positive insights. There was a positive significant relationship between calling and work engagement. Calling had also moderated, with a positive significant result, the relationship between AL and work engagement. Future research should focus on the importance of perceived AL within the education public services, and the significant effect of calling on work engagement. Our study suggests that organizations could promote employee calling work orientation, stimulate leaders to become more authentic, and improve positively organization performance.
Insertion bias and purifying selection of retrotransposons in the Arabidopsis thaliana genome
Vini Pereira
Genome Biology , 2004, DOI: 10.1186/gb-2004-5-10-r79
Abstract: I investigated the evolutionary dynamics of long terminal repeat (LTR)-retrotransposons in the compact Arabidopsis thaliana genome, using an automated method for obtaining genome-wide, age and physical distribution profiles for different groups of elements, and then comparing the distributions of young and old insertions. Elements of the Pseudoviridae family insert randomly along the chromosomes and have been recently active, but insertions tend to be lost from euchromatic regions where they are less likely to fix, with a half-life estimated at approximately 470,000 years. In contrast, members of the Metaviridae (particularly Athila) preferentially target heterochromatin, and were more active in the past.Diverse evolutionary mechanisms have constrained both the copy number and chromosomal distribution of retrotransposons within a single genome. In A. thaliana, their non-random genomic distribution is due to both selection against insertions in euchromatin and preferential targeting of heterochromatin. Constant turnover of euchromatic insertions and a decline in activity for the elements that target heterochromatin have both limited the contribution of retrotransposon DNA to genome size expansion in A. thaliana.It has become increasingly clear that the activity of transposable elements (TEs) is a major cause of genome evolution. TEs are ubiquitous components of eukaryotic genomes. For example, 22% of the Drosophila melanogaster [1], 45% of the human [2], and up to 80% of the maize [3] genomes consist of TE fossils. TEs have influenced the evolution of cellular gene regulation and function, and have been responsible for chromosomal rearrangements [4]. Variation in genome size and the C-value paradox [5] can be attributed to a large extent to differences in the amount of TEs, particularly of retrotransposons, between the genomes of different species [6]. In plant genomes, large size and structural variation even among closely related species is mainly due to difference
1D 13C-NMR Data as Molecular Descriptors in Spectra — Structure Relationship Analysis of Oligosaccharides
Florbela Pereira
Molecules , 2012, DOI: 10.3390/molecules17043818
Abstract: Spectra-structure relationships were investigated for estimating the anomeric configuration, residues and type of linkages of linear and branched trisaccharides using 13C-NMR chemical shifts. For this study, 119 pyranosyl trisaccharides were used that are trimers of the?α or?β anomers of D-glucose, D-galactose, D-mannose, L-fucose or L-rhamnose residues bonded through a or b glycosidic linkages of types 1→2, 1→3, 1→4, or 1→6, as well as methoxylated and/or N-acetylated amino trisaccharides. Machine learning experiments were performed for: (1) classification of the anomeric configuration of the first unit, second unit and reducing end; (2) classification of the type of first and second linkages; (3) classification of the three residues: reducing end, middle and first residue; and (4) classification of the chain type. Our previously model for predicting the structure of disaccharides was incorporated in this new model with an improvement of the predictive power. The best results were achieved using Random Forests with 204 di- and trisaccharides for the training set—it could correctly classify 83%, 90%, 88%, 85%, 85%, 75%, 79%, 68% and 94% of the test set (69 compounds) for the nine tasks, respectively, on the basis of unassigned chemical shifts.
Automated paleontology of repetitive DNA with REANNOTATE
Vini Pereira
BMC Genomics , 2008, DOI: 10.1186/1471-2164-9-614
Abstract: Here I present REANNOTATE, a computational tool to process REPEATMASKER annotation for automated i) defragmentation of dispersed repetitive elements, ii) resolution of the temporal order of insertions in clusters of nested elements, and iii) estimating the age of the elements, if they have long terminal repeats. I have re-annotated the repetitive content of human chromosomes, providing evidence for a recent expansion of satellite repeats on the Y chromosome and, from the retroviral age distribution, for a higher rate of evolution on the Y relative to autosomes.REANNOTATE is ready to process existing annotation for automated evolutionary analysis of all types of complex repeats in any genome. The tool is freely available under the GPL at http://www.bioinformatics.org/reannotate webcite.Repeats of high sequence complexity – mostly transposable elements (TEs) – account for a large portion of many eukaryotic genomes. In humans they comprise almost half of the (cytologically euchromatic) genome [1], and in some plants (e.g. maize) most of the DNA (>70%) is repetitive [2-4]. In addition to genome size evolution, repetitive sequences are fundamentally implicated in structural and functional genome evolution. Sequence similarity and complementarity form the basis of many biochemical reactions involving nucleic acids. Hence the occurrence of repeated sequences may mediate genome, epigenome and transcriptome interactions, such as chromosomal rearrangements [5-7], centromere [8] and telomere [9] function, and chromatin remodelling and gene silencing mediated by repeat-induced small RNAs [10-15]. In addition to the effects of sequence repetition, TE insertions may have sequence-specific phenotypic consequences. For instance, they encode regulatory signals that can potentially affect gene expression [16-19].Given the importance of repetitive DNA sequences for genome structure and evolution, systematic annotation of repeats is essential for inferring biological organisation and f
Caso endoscópico
Pereira,Fernando;
Nascer e Crescer - Revista do Hospital de Crian?as Maria Pia , 2011,
Abstract: an eight-year-old boy, with weaver syndrome, was referred to the gastroenterology unit because of abdominal pain, more frequently localized to the right lower abdominal quadrant, weight loss and diarrhoea (without faecal mucus or blood) throughout the last three months. slight macrocephly and pain on the right iliac fossa were the only abnormalities on physical examination. there was mild anemia, and elevation of globular sedimentation rate, protein c and orosomucoide. asca and faecal calprotectine were above the normal range. upper endoscopy and colonos-copy were normal, and videocapsule enteroscopy showed lesions suggestive of crohn’s disease of the ileum. the patient was treated with messalazine and became asymptomatic. laboratory parameters were normal within two months.
Caso endoscópico
Pereira,Fernando;
Nascer e Crescer - Revista do Hospital de Crian?as Maria Pia , 2011,
Abstract: we present the case of a eight-year old boy with microcytic hypochromic anemia, who refused to ingest meat products for the last years. he had a good response to therapy with oral iron supplements, but with further recurrence of symptoms after stopping treatment. investigation by haematology was inconclusive. the upper endoscopy showed severe peptic esophagitis with stenosis. the patient was treated with proton pump inhibitors, dilation of the stenosis and surgery (nissen fundoplication), with good outcome.
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