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Search Results: 1 - 10 of 127164 matches for " PeiGuo Li "
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杂交水稻及其亲本光合特性的研究 Ⅰ.功能叶片叶绿素含量、叶绿素-蛋白复合物及诱导荧光动力学

Guo Peiguo,Li Mingqi,

热带亚热带植物学报 , 1996,
Abstract: 研究了杂交水稻青优159和广优四号及其亲本功能叶片的光合速率、叶绿素含量、叶绿素-蛋白复合物及诱导荧光动力学特性.这二个杂交水稻的光合速率分别高于其亲本,其超亲优势分别为18.72%和18.2%,平均优势分别为29.6%和26.2%.杂交水稻功能叶片的叶绿素-蛋白复合物在650nm和675nm处光密度扫描峰面积具有明显的杂种优势,并与光合速率之间有较密切的正相关关系;叶绿素诱导荧光动力学特征参数Fv/Fm和Fv/Fo比值超过其高值亲本,具有杂种优势;杂交水稻功能叶片的叶绿素含量没有明显的杂种优势,光合速率与叶绿素含量之间没有明显的相关关系。而杂交水稻功能叶片的叶绿素a/b比值均低于其亲本,并且叶绿素a/b比值与光合速率呈较为密切负相关.
Iterative Solutions of Singular Boundary Value Problems of Third-Order Differential Equation
Zhang Peiguo
Boundary Value Problems , 2011,
Abstract: By using the cone theory and the Banach contraction mapping principle, the existence and uniqueness results are established for singular third-order boundary value problems. The theorems obtained are very general and complement previous known results.
Existence of positive solutions for nonlocal second-order boundary value problem with variable parameter in Banach spaces
Zhang Peiguo
Fixed Point Theory and Applications , 2011,
Abstract: By obtaining intervals of the parameter λ, this article investigates the existence of a positive solution for a class of nonlinear boundary value problems of second-order differential equations with integral boundary conditions in abstract spaces. The arguments are based upon a specially constructed cone and the fixed point theory in cone for a strict set contraction operator. MSC: 34B15; 34B16.
Iterative Solutions of Singular Boundary Value Problems of Third-Order Differential Equation
Peiguo Zhang
Boundary Value Problems , 2011, DOI: 10.1155/2011/483057
Screening of Multiple Drug Resistant Genes of Eimeria tenella Infesting Chicken in China
Yanying Zhang, Qinghui Jia, Xiangzhai Zhang, Yunyu Li, Peiguo Li*, Wenxiang Zhang, Juan Chen and Wenjin Zhu
Pakistan Veterinary Journal , 2013,
Abstract: Total RNA of Eimeria tenella drug-resistant strains from Qinhuangdao, Tanshan and Shijiazhuang were extracted with Trizol. DDRT-PCR was established by 3 anchored primers and 20 arbitrary primers. The products of PCR were analyzed on the denaturing polyacrylamide gels by silver-straining. Twenty-six differential bands were excised from the gels and reamplified with the same sets of primers. The products were purified, and 12 differential bands (20~50ng) were recuperated. Twelve plasmid cloning identification differential bands were identified by the dot-blot hybridization. The results showed that 3 bands were positive. And then the positive cloning was sequenced and compared to homology. The results showed that through comparison of the nucleotide acid sequence, the similarity was 99% among the sequence S116 from mRNA of Shijiazhuang multiple-resistant strain with the sequence 882bp length in the first chromosome of E. tenella in Genbank and Sanger, which was an unknown protein. The sequence of T311 and Q19 from mRNA of Tangshan and Qinhuangdao multiple-resistant strains were unknown sequences. These novel cDNA fragments associated with drug resistance might be involved in the process of the drug resistances of E. tenella.
Detection of the Major Macrolide Resistance Genes in Streptococcus suis Serotype 2 Isolates in Hebei Province China
Ping Rui,PeiGuo Li,Zengjun Ma,QinYe Song,CaiRan Yang,Ping Shen,SuMin Pan,Yufang Guo
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.781.784
Abstract: To investigate the distribution of ermB, ermA and mefA related to the erythromycin resistance gene, 32 drug-resistant Streptococcus suis type 2 isolates from different areas in Hebei province were studied to detect the ermB, ermA and mefA genes by PCR amplification. The results showed that among the drug-resistant 32 strains, 100% (32/32) amplified ermB gene, 21.88% (7/32) isolates contained ermA gene and none of these strains was positive for mefA gene. The researchers concluded that erythromycin resistance mechanism of pig Streptococcus isolates is mainly mediated by ermB gene. Nucleotide sequences comparison showed that the ermB gene of 20 strains and the nucleotide sequences in the GenBank had the sequence similarity of 95-100%. Compared with the reference sequence of AJ972604.1, Ser to Asn mutation at position 100 and Arg to His mutation at position 118 of ermB was mainly detected in the 20 strains. The researchers concluded that the ermB gene is relatively stable.
Hematology and Serum Chemistry Values in Adult Minks in Hebei Province, China
Ping Rui,Zengjun Ma,Xiangzhai Zhang,Peiguo Li,Guangping Gao,Zongze Yang,Jinhui Zhang
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.662.664
Abstract: This study examined hematology and serum chemistry status of adult American minks (Mustla vison), living in the Changli fur farms of Hebei province, Eastern China to provide important baseline data for clinical diagnosis and breeding in the corresponding animal species. The 41 values were compared between males (n = 10) and female (n = 10) minks. The results showed that gender influenced (p<0.05) serum biochemistry values. Male minks had higher (p<0.05) Creatin Kinase (CK) and Alanine aminotrotransferase (ALT) and lower (p<0.05) Amylase (AM) than females. However, the hematology values were not different by genders.
Allelic Variations of a Light Harvesting Chlorophyll A/B-Binding Protein Gene (Lhcb1) Associated with Agronomic Traits in Barley
Yanshi Xia, Zhengxiang Ning, Guihua Bai, Ronghua Li, Guijun Yan, Kadambot H. M. Siddique, Michael Baum, Peiguo Guo
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0037573
Abstract: Light-harvesting chlorophyll a/b-binding protein (LHCP) is one of the most abundant chloroplast proteins in plants. Its main function is to collect and transfer light energy to photosynthetic reaction centers. However, the roles of different LHCPs in light-harvesting antenna systems remain obscure. Exploration of nucleotide variation in the genes encoding LHCP can facilitate a better understanding of the functions of LHCP. In this study, nucleotide variations in Lhcb1, a LHCP gene in barley, were investigated across 292 barley accessions collected from 35 different countries using EcoTILLING technology, a variation of the Targeting Induced Local Lesions In Genomes (TILLING). A total of 23 nucleotide variations were detected including three insert/deletions (indels) and 20 single nucleotide polymorphisms (SNPs). Among them, 17 SNPs were in the coding region with nine missense changes. Two SNPs with missense changes are predicted to be deleterious to protein function. Seventeen SNP formed 31 distinguishable haplotypes in the barley collection. The levels of nucleotide diversity in the Lhcb1 locus differed markedly with geographic origins and species of accessions. The accessions from Middle East Asia exhibited the highest nucleotide and haplotype diversity. H. spontaneum showed greater nucleotide diversity than H. vulgare. Five SNPs in Lhcb1 were significantly associated with at least one of the six agronomic traits evaluated, namely plant height, spike length, number of grains per spike, thousand grain weight, flag leaf area and leaf color, and these SNPs may be used as potential markers for improvement of these barley traits.
Single Nucleotide Polymorphisms in HSP17.8 and Their Association with Agronomic Traits in Barley
Yanshi Xia, Ronghua Li, Zhengxiang Ning, Guihua Bai, Kadambot H. M. Siddique, Guijun Yan, Michael Baum, Rajeev K. Varshney, Peiguo Guo
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0056816
Abstract: Small heat shock protein 17.8 (HSP17.8) is produced abundantly in plant cells under heat and other stress conditions and may play an important role in plant tolerance to stress environments. However, HSP17.8 may be differentially expressed in different accessions of a crop species exposed to identical stress conditions. The ability of different genotypes to adapt to various stress conditions resides in their genetic diversity. Allelic variations are the most common forms of genetic variation in natural populations. In this study, single nucleotide polymorphisms (SNPs) of the HSP17.8 gene were investigated across 210 barley accessions collected from 30 countries using EcoTILLING technology. Eleven SNPs including 10 from the coding region of HSP17.8 were detected, which form nine distinguishable haplotypes in the barley collection. Among the 10 SNPs in the coding region, six are missense mutations and four are synonymous nucleotide changes. Five of the six missense changes are predicted to be deleterious to HSP17.8 function. The accessions from Middle East Asia showed the higher nucleotide diversity of HSP17.8 than those from other regions and wild barley (H. spontaneum) accessions exhibited greater diversity than the cultivated barley (H. vulgare) accessions. Four SNPs in HSP17.8 were found associated with at least one of the agronomic traits evaluated except for spike length, namely number of grains per spike, thousand kernel weight, plant height, flag leaf area and leaf color. The association between SNP and these agronomic traits may provide new insight for study of the gene's potential contribution to drought tolerance of barley.
Efficacy of Radiofrequency Hyperthermia Combined with Chemotherapy in Treatment of Malignant Pericardial Effusion Caused by Lung Cancer
Pengfei LUO,Peiguo CAO,Zhiping YAO
Chinese Journal of Lung Cancer , 2011, DOI: 10.3779/j.issn.1009-3419.2011.07.06
Abstract: Background and objective Malignant pericardial effusion is one of the serious complications of lung cancer and lack effective treatment methods. The aim of this study is to evaluate the efficacy and safety of radiofrequency hyperthermia combined with chemotherapy for patients with malignant pericardial effusion caused by lung cancer. Methods Fifty-five patients with malignant pericardial effusion caused by lung cancer were divided into hyperthermia combined with chemotherapy group (combined therapy group) and chemotherapy group. The combined therapy group was treated with radiofrequency hyperthermia after the pericardiocentesis and intracavitary injection (cisplatin 20 mg and dexamethasone 5 mg), when patients’ general state of health improved, systemic chemotherapy was performed. The chemotherapy group was treated only with intracavitary injection and systemic chemotherapy. Intracavitary chemotherapy was performed for 1-6 times (average 3 times). Hyperthermia was performed twice per week with an average of 6 times following intracavitary and systemic chemotherapy. The temperature of intracavitary was 40.5 oC-41.5 oC for 60 min during the hyperthermia periods. Systemic chemotherapy consists of cisplatin (75 mg/m2) and vinorelbine (50 mg/m2). Results The complete remission rate (CR) of malignant pericardial effusion was 54.3% and the response rate (RR) was 91.4% in the combined therapy group, while the rates of CR and RR of chemotherapy group were 25.0% and 70.0%, and the differences of CR and RR between the two groups were significant (P<0.05). After treatment, the quality of life improved significantly in both groups, but the combined therapy group had a higher KPS score than in the chemotherapy group (P<0.05). The adverse events associated with the chemotherapy included gastrointestinal toxicity and myelosup-pression, and there were no significant differences between the two groups. The main side effects associated with radiofrequency hyperthermia included local skin ache (8.6%) and induration of subcutaneous fat (5.7%). Conclusion Radiofrequency hyperthermia combined with chemotherapy is effective in treatment of malignant pericardial effusion and can significantly improve the quality of life of patients without increasing the toxicity of chemotherapy, and side effects could be tolerable.
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