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Search Results: 1 - 10 of 372 matches for " Pavla Pekárová "
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Estimating Flash Flood Peak Discharge in Gidra and Parná Basin: Case Study for the 7-8 June 2011 Flood
Pavla Pekárová, Ale Svoboda, Pavol Miklánek, Peter koda, Dana Halmová, Ján Pekár
Journal of Hydrology and Hydromechanics , 2012, DOI: 10.2478/v10098-012-0018-z
Abstract: We analyzed the runoff and its temporal distribution during the catastrophic flood events on river Gidra (32.9 km2) and Parná (37.86 km2) of the 7th June 2011. The catchments are located in the Small Carpathian Mountains, western Slovakia. Direct measurements and evaluation of the peak discharge values after such extreme events are emphasized in the paper including exceedance probabilities of peak flows and of their causal flash rainfall events. In the second part of the paper, plausible modeling mode is presented, using the NLC (Non Linear Cascade) rainfall-runoff model. Several hypothetical extreme flood events were simulated by the NLC model for both rivers. Also the flood runoff volumes are evaluated as basic information on the natural or artificial catchment storage.
Prediction of Water Quality in the Danube River Under extreme Hydrological and Temperature Conditions
Pavla Pekárová , Milan Onderka , Ján Pekár , Peter Ron ák , Pavol Miklánek
Journal of Hydrology and Hydromechanics , 2009, DOI: 10.2478/v10098-009-0001-5
Abstract: One of the requirements imposed by the Water Framework Directive (WFD, 2000/60/EC) is to analyze and predict how quality of surface waters will evolve in the future. In assessing the development of a stream's pollution one must consider all sources of pollution and understand how water quality evolves over time. Flow and water temperature regime of a stream or river are the main factors controlling the extent to which deterioration of a stream's water quality can propagate under constant input from pollution sources. In addition, there is ever increasing public concern about the state of the aquatic environment. Decision makers and scientists involved in water management call for studies proposing simulation models of water quality under extreme natural hydrologic and climatic scenarios. Also, human impact on water resources remain an issue for discussion, especially when it comes to sustainability of water resources with respect to water quality and ecosystem health. In the present study we investigate the long-term trends in water quality variables of the Danube River at Bratislava, Slovakia (Chl-a, Ca, EC, SO2-, Cl-, O2, BOD5, N-tot, PO4-P, NO3-N, NO2-N, etc.), for the period 1991-2005. Several SARIMA models were tested for the long-term prediction of selected pollutant concentrations under various flow and water temperature conditions. In order to create scenarios of selected water quality variables with prediction for 12 months ahead, three types of possible hydrologic and water temperature conditions were defined: i) average conditions - median flows and water temperature; ii) low flows and high water temperature; and iii) high flows and low water temperature. These conditions were derived for each month using daily observations of water temperature and daily discharge readings taken in the Danube at Bratislava over the period 1931-2005 in the form of percentiles (1th-percentile, median, 99th-percentile). Once having derived these extreme-case scenarios, we used selected Box-Jenkins models (with two regressors - discharge and water temperature) to simulate the extreme monthly water quality variables. The impact of natural and man-made changes in a stream's hydrology on water quality can be readily well simulated by means of autoregressive models.
Investigation of Interaction of Some Chalcones and Cyclic Chalcone Analogues with Outer Mitochondrial Membrane by UV-VIS and Fluorescence Spectroscopy  [PDF]
Vladimíra Tome?ková, Miroslava ?tefani?inová, Beáta Veliká, Krisztina Fodor, Pal Perjési, Marek Stupák, Juraj Guzy, ?tefan Tóth Jr, Tímea Pekárová
Spectral Analysis Review (SAR) , 2013, DOI: 10.4236/sar.2013.11001
Abstract: Interaction of the synthetic chalcones (1b,1c) and their cyclic analogues (2b,2c) with bovine (BSA) and human serum albumin (HSA) as well as with rat liver mitochondria (RLM) was studied by fluorescence spectroscopy. The maxima of emission fluorescence spectra were changed only in the case of 2b and 2c during interaction with BSA, HSA as well as mitochondrial outer membrane showing a slight hypsochromic shift and decrease of fluorescence. Interaction of the methoxy-(1b,2b) and the dimethylamino-substituted (1c,2c) compounds with outer mitochondrial membrane were studied by fluorescence polarization. Fluorescence polarization of 1b in the presence of the two proteins and mitochondria was found to be unchanged. Under similar conditions (2b,1c,2c) showed continuously increasing fluorescence polarization signal during
the 30 minute period of investigations. Since fluorescence polarization supposes that as a result of binding these substances to proteins and lipids. Compound 2c displayed a continuous increase of fluorescence po
larization signal in the presence of proteins (BSA, HSA), yeast cytoplasm (YC) and mitochondria (YM and RLM). This compound displayed a significant cytotoxic effect. This pattern of interaction with proteins might be one of the contributing vectors of the observed cytotoxicity against several human carcinoma cell lines.

Interactions of an Arabidopsis RanBPM homologue with LisH-CTLH domain proteins revealed high conservation of CTLH complexes in eukaryotes
Eva Toma?tíková, Věra Cenklová, Lucie Kohoutová, Beáta Petrovská, Lenka Váchová, Petr Halada, Gabriela Ko?árová, Pavla Binarová
BMC Plant Biology , 2012, DOI: 10.1186/1471-2229-12-83
Abstract: Based on sequence similarity, we identified a homologue of the human RanBPM in Arabidopsis thaliana. AtRanBPM protein has highly conserved SPRY, LisH, CTLH and CRA domains. Cell fractionation showed that endogenous AtRanBPM or expressed GFP-AtRanBPM are mainly cytoplasmic proteins with only a minor portion detectable in microsomal fractions. AtRanBPM was identified predominantly in the form of soluble cytoplasmic complexes ~230 – 500?kDa in size. Immunopurification of AtRanBPM followed by mass spectrometric analysis identified proteins containing LisH and CRA domains; LisH, CRA, RING-U-box domains and a transducin/WD40 repeats in a complex with AtRanBPM. Homologues of identified proteins are known to be components of the C-terminal to the LisH motif (CTLH) complexes in humans and budding yeast. Microscopic analysis of GFP-AtRanBPM in vivo and immunofluorescence localization of endogenous AtRanBPM protein in cultured cells and seedlings of Arabidopsis showed mainly cytoplasmic and nuclear localization. Absence of colocalization with γ-tubulin was consistent with the biochemical data and suggests another than a centrosomal role of the AtRanBPM protein.We showed that as yet uncharacterized Arabidopsis RanBPM protein physically interacts with LisH-CTLH domain-containing proteins. The newly identified high molecular weight cytoplasmic protein complexes of AtRanBPM showed homology with CTLH types of complexes described in mammals and budding yeast. Although the exact functions of the CTLH complexes in scaffolding of protein degradation, in protein interactions and in signalling from the periphery to the cell centre are not yet fully understood, structural conservation of the complexes across eukaryotes suggests their important biological role.Ras-related nuclear proteins (Rans) are abundant small GTPases, associated with Ran-specific nuclear GEFs (guanine nucleotide exchange factors), cytoplasmic GAPs (GTPase activating proteins), and with RanBP1 (Ran binding protein 1) t
A novel method for the detection of liver damage using fluorescence of hepatic mitochondria in a rat model following ischaemia/reperfusion injury of the small intestine
Vladimíra Tome ková,Miroslava tefani inová,Miroslava Bilecová-Rabajdová,Eli ka Kri áková,Marián Tome ko, tefan Tóth,Tímea Pekárová,Mária Mareková
Spectroscopy: An International Journal , 2011, DOI: 10.3233/spe-2011-0545
Abstract: We present a novel method of assessing damage to the liver using fluorescence analysis of hepatic mitochondria following ischaemia/reperfusion of the small intestine in a rat model. This work is of substantial importance in understanding the syndrome of multiorgan failure after ischemia/transplantation of the small intestine. Mitochondria were isolated from six sample groups that had undergone three different experimental treatments: a control group; a treatment with ischaemia followed by reperfusion of the small intestine (IRx); and, a one hour ischaemia followed by reperfusion after transplant of the small intestine (TRx). The IR treatment was further subdivided into three groups: 1, 24 h and 30 days reperfusion – IR1, IR24, IR720, respectively. Concomitantly, the TR treatment was further subdivided: one group underwent a 1 h reperfusion and another group a 6 h reperfusion following ischaemia and transplant – groups TR1 and TR6, respectively. Once treatment had been undergone, mitochondria were isolated and all five experimental groups – IR1, IR24, IR720, Tr1, Tr6 – and their emission matrices were analysed compared with that of the control group (C). Comparing fluorescence values in zone A of all experimental groups with those of the control group indicated a reduction in aromatic amino acids in the mitochondria of all experimental groups. Comparison of fluorescent zone B of experimental groups with the control group identified a lack of oxygen in samples IR1, IR24, which was indicated though an increase in the fluorescence of the reduced pyridine nucleotide NADH
Calculation of Constitutive Parameters from Electric and Magnetic Field Measurements in an Anisotropic Medium with a Triaxial Instrument  [PDF]
Ertan Pek?en
International Journal of Geosciences (IJG) , 2013, DOI: 10.4236/ijg.2013.41007
Abstract:

A hypothetical electric and magnetic induction tensor is considered in an anisotropic medium. The sources are magnetic dipoles. In such a medium, constitute parameters can be calculated by combining electric and magnetic field measurements. Constitutive parameters are not a scalar in this case. They are tensors, so parameters have at least both horizontal and vertical components in a uniaxial medium. These calculated parameters from the field measurement are horizontal and vertical conductivity, permittivity, and magnetic permeability. Operating frequency range is also quite large. It is up to 4 GHz. A hypothetical instrument should measure gradient fields both electric and magnetic types as well.

Nové zdroje inovací - vyzvy pro eskou ekonomiku a její postavení v globální ekonomice
Pavla í alová
Ergo , 2011, DOI: 10.2478/v10217-011-0003-6
Abstract: The economic crisis and current recession have accelerated the transition of the globalization towards its next phase. So called globalization 2.0 has not only increased the interconnectedness of the global economy, but also shifted the economic power towards non-Western groups and emerging economies - not just in case of manufacturing, but particularly in case of consumption. Demand and supply centres are moving and often again are being located in the same locality. Growing emerging economies and their numerous populations are believed to become the future demand growth drivers. And thus, new sources of innovations - customers drives most innovations - coming from emerging markets to the developed ones. The aim of this article is to shortly assess what is the position of Czechia regarding these new global trends and what are the implication for innovation and competitiveness support.
Open Access Week 2011 in the Czech Republic (Report)
Pavla Rygelová
Liber Quarterly : The Journal of European Research Libraries , 2012,
Abstract: The awareness of open access is slowly rising in the Czech academic environment. Both green open access and gold open access have broken through thanks to Open Access Week, which has for the second time been organized by twelve Czech university libraries, the National Technical Library, and the Library of the Academy of Sciences of the Czech Republic, under the auspices of the Association of Libraries of Czech Universities.
Determinants which Affect Liquid Asset Ratio of Czech and Slovak Commercial Banks
Pavla Vodová
Financial Assets and Investing , 2013,
Abstract: The aim of this paper is to find out determinants which affect liquid asset ratio of Czech and Slovak commercial banks. The data cover the period from 2001 to 2010. We consider four bank specific factors and nine macroeconomic factors. Results of panel data regression analysis showed that although Czech Republic and Slovak Republic have a lot in common, different factors determined banks′ liquid assets in individual countries. The liquid asset ratio of Czech banks increases with increase of capital adequacy, with depreciation of Czech koruna and with worsening quality of credit portfolio. Liquidity of Slovak banks decreases with size of the bank, with higher capital adequacy, higher bank liquidity and during periods of financial crisis. Liquidity of Slovak banks is also positively related to economic cycle.
Carta de la Dra. Alicia Rovó
Alicia Rovó
Acta bioqu?-mica cl?-nica latinoamericana , 2006,
Abstract:
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