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Search Results: 1 - 10 of 19880 matches for " Paulo Yoshio KAGEYAMA "
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Comparison between multivariate methods applied for the evaluation of genetic divergence in Cacao (Theobroma cacao L.)
Dias, Luiz Ant?nio dos Santos;Kageyama, Paulo Yoshio;
Brazilian Archives of Biology and Technology , 1998, DOI: 10.1590/S1516-89131998000200005
Abstract: several multivariate methods have been used in divergence analyses of populations. consistency and relative association among four methods were assessed using a 5 x 5 complete-diallel data involving cacao cultivars. over a 5-year period, five cultivars were analyzed based upon five yield components. in assessing the divergence of parents only the data obtained from five cacao cultivars were analyzed. four multivariate statistics presented close association when considered in pairs, in this case the mahalanobis' (d2) with the mean euclidean distance obtained from canonical variates (dcv), and mean euclidean distance (de) with the mean euclidean distance obtained from principal components (dpc). in both cases, high correlations (r > 0.95) were obtained. however, a weak association was detected between d2 and de and between dpc and dcv (0.50 and 0.66, respectively). thus, in studies on genetic divergence, statistics considering the error variance-covariance matrix should be preferred whenever its estimate is possible.
Estrutura genética e sistema de acasalamento de Piper hispidinervum
Wadt, Lúcia Helena de Oliveira;Kageyama, Paulo Yoshio;
Pesquisa Agropecuária Brasileira , 2004, DOI: 10.1590/S0100-204X2004000200008
Abstract: long pepper (piper hispidinervum c. dc.) is a small tree with high commercial value found in areas under anthropic influence in the state of acre, brazil. the genetic structure and mating system of p. hispidinervum were evaluated using rapd markers. the genetic diversity within and between natural populations were evaluated in 13 populations in the basin acre river - western brazilian amazon. twenty five open-pollinating families in a population located in assis brasil were evaluated to estimate the preferential crossing rate. genetic diversity was observed, revealing that this species is spatially structured according to a pattern of isolation by distance. most of the genetic variability was found within populations, and the variation between populations was also high (qp = 0.28). two distinct groups were formed, based on genetic distances (fst), representing the upper acre and lower acre watersheds. by amova, 20.61% of the total variability occurs between those two watershed regions. the multilocus crossing rate was estimated at 1.033, the estimate of the inbreeding coefficient (f) did not vary from zero, and the crosses preferentially occurred between unrelated individuals.
Estrutura genética espacial em popula??es naturais de Machaerium villosum Vog. (Leguminosae) na regi?o de Moji-Gua?u, SP, Brasil
Brazilian Journal of Botany , 2000, DOI: 10.1590/S0100-84042000000200010
Abstract: a spatial autocorrelation study of enzyme loci detected by starch gel electrophoresis was performed to verify the occurrence of spatial genetic structure within two natural populations of machaerium villosum vog. the sampled populations were termed "antropic model (ma)" and "natural model (mn)" and they are situated in campininha farm areas, at moji-gua?u municipality, 22°10'43''-22°18'19'' s and 47°8'5"-47°11'34" w, in the state of s?o paulo. ten polymorphic loci in the ma population and nine polymorphic loci in the mn population were assessed by moran's i autocorrelation statistic. no spatial autocorrelation was detected among individuals within sampled populations. results are in line with other studies in woody species from tropical rain forest.
Estrutura genética espacial de duas popula??es naturais de Myracrodruon urundeuva M. Allem?o na regi?o semi-árida, Brasil
Lacerda, Cristina Maria Batista de;Kageyama, Paulo Yoshio;
Revista árvore , 2003, DOI: 10.1590/S0100-67622003000200004
Abstract: to determine whether genotype spacial distribution is random or structured, spacial genotypic autocorrelation was used to compare two natural populations of myracrodruon urundeuva in the semi-arid region of northeastern brazil (esta??o ecológica do seridó/rn - 1166,38/ha and sítio mata dos alves/pb - 84/ha). the former is characteristic of preserved areas, while the latter is found in areas with marked anthropogenic degradation. the program "autocorr" was used for the analysis. the autocorrelation was run on polymorphic loci detected in the adult individuals. two alleles were analyzed at the esta??o ecológica do seridó and 4 at the sítio mata dos alves. the three methods used for analysis were: connection of gabriel (ig), closest neighbor (ivmp) and pre-established distance comparisons. the results obtained for ig and ivmp (pgm2: 0,032 and 0,236; est-1: 0,263 and 0,242, respectively) in the ecological station sample/populations and ig and ivmp (pgm-1: -0,349 and -0,288; pgm-2: -0,341 and 0,278; est-1: -0,349 and -0,284 and mdh-1: -0,345 and -0,282, respectively) in the farm sample/populations. the results did not indicate the presence of special genetic structure, making it possible to hypothesize that there is an internal randomized genotypic distribution of the genotype. the same was detected for the pre established distance comparisons. the results maintained the same pattern found in some natural populations of tropical tree species studied.
Sistema de reprodu??o em popula??es de Eschweilera ovata (Cambess.) Miers
Gusson, Eduardo;Sebbenn, Alexandre Magno;Kageyama, Paulo Yoshio;
Revista árvore , 2006, DOI: 10.1590/S0100-67622006000400001
Abstract: the mating system of two populations of eschweilera ovata was studied by allozymes analysis of progeny arrays using the mixed-mating model and correlated mating model. deviations from mixed-mating model were evident from differences in pollen and ovule allele frequencies and allele frequency heterogeneity of pollen pools that fertilized the different trees. the multilocus outcrossing rate was high in both camarugipe (tm=0.999±0.004) and itaparica populations (tm=0.985±0.023). the high variation in individual outcrossing rate (t ranged from 0.320 to 1.000) indicated that the species is not self-incompatible. positive differences and significantly different from zero between multilocus and single locus outcrossing rate were detected, indicating biparental inbreeding in both camarugipe (tm - ts=0.066±0.014) and itaparica populations (tm - ts=0.073±0.016) and possible spatial genetic structuring. higher values of correlated mating were detected in the populations (camarugipe, rp=0.577±0.088; itaparica rp=0.423±0.070), showing that the families consisted mainly of half-sib and full-sib mixtures. the coancestry coefficient within families from both populations (camarugipe, qxy=0.211; itaparica qxy=0.191) was higher than the expected in half-sib families (0.125). the results were discussed from the point of view of sampling for improvement, genetic conservation and seed collection aiming at environmental recovery.
Estrutura genética e sistema de acasalamento de Piper hispidinervum
Wadt Lúcia Helena de Oliveira,Kageyama Paulo Yoshio
Pesquisa Agropecuária Brasileira , 2004,
Abstract: A pimenta-longa (Piper hispidinervum C. DC.) arbusto encontrado em áreas antropizadas no Estado do Acre, possui expressiva importancia econ mica decorrente da presen a de safrol em seu óleo essencial. O objetivo deste trabalho foi avaliar a estrutura genética e o sistema de acasalamento dessa espécie, utilizando marcadores RAPD (polimorfismo de DNA amplificado ao acaso). A diversidade genética entre e dentro de popula es naturais foi avaliada em 13 popula es do Vale do Rio Acre, distribuídas nas Regi es do Baixo e Alto Acre. A taxa preferencial de cruzamento foi estimada em 25 famílias de poliniza o livre de uma popula o do Município de Assis Brasil, Acre. A espécie apresentou diversidade genética estruturada no espa o segundo um padr o de isolamento por distancia. A maior parte da variabilidade genética foi encontrada dentro das popula es, porém a diferencia o entre popula es, como um todo, foi alta (qP = 0,28). O agrupamento das popula es, pela distancia genética (fST) entre elas, mostrou dois grupos distintos, os quais representam as regi es do Alto Acre e Baixo Acre. A AMOVA mostrou que 20,61% da variabilidade total está entre essas duas regi es. A taxa de cruzamento multilocos foi estimada em 1,033, evidenciando que a espécie é alógama. A estimativa do coeficiente de endogamia F n o diferiu de zero e os cruzamentos ocorreram preferencialmente entre indivíduos n o-aparentados.
Heran a e desequilíbrio de liga o em nove locos isoenzimáticos de Eremanthus erythropappus. Genetic inheritance and linkage desequilibrium in nine isozyme loci of Eremanthus erythropappus.
Sybelle BARREIRA,Alexandre Magno SEBBENN,Paulo Yoshio KAGEYAMA
Revista do Instituto Florestal , 2005,
Abstract: A heran a e o equilíbrio genotípicoforam estudados em sete sistemas isoenzimáticos(ACP, EST, 6PGDH, PGM, PGI, DIA e G6PDH),codificando sete locos em Eremanthus erythropappus.A análise foi baseada na segrega o de progênies depoliniza o aberta de árvores maternas heterozigotase o desequilíbrio foi estudado com base na medida dedesequilíbrio genotípico de Burrows. O locoG6pdh n o p de ter sua heran a estudada porquen o foram detectadas árvores maternas heterozigóticaspara este loco. As outras seis enzimas erampolimórficas e foram encontradas árvores maternasheterozigóticas. A heran a mendeliana de seislocos (Acp, Est, 6pgdh, Pgi, Pgm e Dia) foiconfirmada pelo ajuste das freqüências gaméticasmaternas observadas às esperadas pelo modelo desegrega o 1:1. O desequilíbrio de liga o foiexaminado para 21 pares de combina es de locos.Um único par de locos parece estar emdesequilíbrio de liga o, Dia/G6pdh.The inheritance and linkagedisequilibrium were studied in seven enzymesystems (ACP, EST, 6PGDH, PGM, PGI, DIA eG6PDH), codifying for seven loci in Eremanthuserythropappus. The segregation analysis was basedon the segregation of open-pollinated offspringsfrom heterozygous mother-trees and the linkagedisequilibrium was studied using Burrowsgenotypic disequilibrium measure. The inheritanceof G6pdh locus was not studied because noheterozygous mother-tree was detected. Anothersix enzymes were polymorphic and heterozygousmother-tree could be found. Mendelian inheritancewas confirmed for all six loci by the fitting of theobserved maternal gametic frequencies to thoseexpected from the 1:1 segregation ratio model.Linkage disequilibrium was examined for as manyas 21 pairs of allozyme loci. A single pair of lociappears to be in gametic disequilibrium,Dia/G6pdh.
Conseqüências do manejo florestal no sistema de reprodu o de Tabebuia cassinoides (Lamarck) A. P. de Candolle. Consequences of forest logging on mating system of Tabebuia cassinoides (Lamarck) A. P. de Candolle.
Alexandre Magno SEBBENN,Eduardo GUSSON,Paulo Yoshio KAGEYAMA
Revista do Instituto Florestal , 2005,
Abstract: Popula es naturais de Tabebuia cassinoides(Lamarck) A. P. de Candolle est o sendo exploradashá muitas décadas, restando hoje apenas poucaspopula es naturais. Este trabalho teve como objetivosestudar as conseqüências da explora o florestal sobreos níveis de diversidade genética e o sistema dereprodu o da espécie. Para isso, comparou-se umapopula o n o explorada (Esta o Ecológica deJuréia-Itatins) e uma explorada pelo corte seletivo(Fazenda Cindomel, Iguape–SP). Os níveis de diversidadegenética observados na popula o n o exploradaforam, em geral, maiores (A = 1,71; e A = 1,29;o H = 0,197; e H = 0,224) do que na explorada(A = 1,71; e A = 1,28; o H = 0,227; e H = 0,217),embora as diferen as n o sejam significativas.Por outro lado, a estimativa da taxa de cruzamentomultiloco foi maior na popula o explorada ( m t = 0,996)do que na n o explorada ( m t = 0,923). As estimativasdas diferen as entre a taxa de cruzamento uniloco emultiloco revelaram a ocorrência de cruzamentos entreparentes nas popula es n o explorada ( s m t - t = 0,044) eexplorada ( s m t - t = 0,046). A estimativa da correla ode paternidade revelou que uma parte consideráveldos cruzamentos foi biparental em ambas as popula es:n o explorada ( p r = 0,570) e explorada ( p r = 0,371),e que parte das progênies s o irm os-completos.De modo geral, os resultados sugerem que aexplora o alterou o sistema de reprodu o da espécie,no sentido de favorecer cruzamentos mais diversificados.Natural population of Tabebuia cassinoides(Lamarck) A. P. de Candolle have been exploredfrom many decades, and actually there is fewremainder populations. The aims of this work wereto verify the consequences of forest logging onlevels of genetic diversity and mating system of theT. cassinoides populations, in a not logging (Juréia-Itatins Ecological Station) and a logging populations(Cindomel Farm, Iguape–SP). The observed levelsof genetic diversity in not logging population(A = 1.71; e A = 1.29; o H = 0.197; e H = 0 .224)were, in general, higher than find out in loggingpopulation (A = 1.71; e A = 1.28; o H = 0.227;e H = 0.217), although the differences are notsignificant. In another hand, the estimate of multilocusoutcrossing rate was higher in the logging( m t =0.996) than natural population ( m t = 0.923).The estimates of the differences betweenmultilocus and single-locus outcrossing raterevealed the mating among relatives in not logging( s m t - t = 0.044) and logging populations( s m t - t = 0.046). The estimates of paternitycorrelation revealed that part of the matings werebipa
Estrutura genética espacial de duas popula es naturais de Myracrodruon urundeuva M. Allem o na regi o semi-árida, Brasil
Lacerda Cristina Maria Batista de,Kageyama Paulo Yoshio
Revista árvore , 2003,
Abstract: Para determinar se a distribui o espacial dos genótipos é aleatória ou se está estruturada, utilizou-se a autocorrela o espacial dos genótipos para investigar dados alozímicos de duas popula es naturais de Myracrodruon urundeuva do semi-árido brasileiro (Esta o Ecológica do Seridó/RN, com 1.166,38/ha e Sítio Mata dos Alves/PB com 84/ha). A primeira popula o encontra-se em áreas mais preservadas, enquanto a segunda está em uma área fragmentada. O programa utilizado foi o "Autocorr". A autocorrela o estimada para os locos polimórficos foi realizada nos indivíduos adultos. Foram analisados dois alelos na Esta o Ecológica do Seridó e quatro no Sítio Mata dos Alves. Utilizaram-se de três métodos para o pareamento de indivíduos a serem comparados: conex o de Gabriel (Ig), vizinho mais próximo (Ivmp) e compara es dentro de classes de distancias preestabelecidas. Os resultados obtidos para Ig e Ivmp (Pgm-2: 0,032 e 0,236; Est-1: 0,263 e 0,242, respectivamente) na esta o ecológica e Ig e Ivmp (Pgm-1: -0,349 e -0,288; Pgm-2: -0,341 e-0,278; Est-1: -0,349 e -0,284 e Mdh-1: -0,345 e 0,282, respectivamente) no sítio n o mostraram a presen a de estrutura o genética espacial, o que possibilita pressupor que exista uma distribui o aleatória dos genótipos dentro delas. O mesmo foi detectado para as compara es dentro de classes de distancias preestabelecidas. Os resultados mantiveram o mesmo padr o encontrado para algumas popula es naturais de espécies arbóreas tropicais já estudadas.
Spatial genetic structure within natural populations of Machaerium villosum Vog. (Leguminosae) at Moji-Gua u region, SP, Brazil
Brazilian Journal of Botany , 2000,
Abstract: A spatial autocorrelation study of enzyme loci detected by starch gel electrophoresis was performed to verify the occurrence of spatial genetic structure within two natural populations of Machaerium villosum Vog. The sampled populations were termed "Antropic Model (MA)" and "Natural Model (MN)" and they are situated in Campininha Farm areas, at Moji-Gua u municipality, 22°10'43''-22°18'19'' S and 47°8'5"-47°11'34" W, in the state of S o Paulo. Ten polymorphic loci in the MA population and nine polymorphic loci in the MN population were assessed by Moran's I autocorrelation statistic. No spatial autocorrelation was detected among individuals within sampled populations. Results are in line with other studies in woody species from tropical rain forest.
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