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Search Results: 1 - 10 of 59353 matches for " Paulo Rodrigues-Santos "
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Genotipagem do Helicobacter pylori no Carcinoma Gástrico e Gastrite Crónica
Silva,Maria Reis; Oliveira,Carla; Rodrigues-Santos,Paulo; Carvalho,Lina;
Jornal Português de Gastrenterologia , 2011,
Abstract: helicobacter pylori (hp) infection is the major cause of chronic active gastritis type b and a pre-neoplasic condition to the development of gastric carcinoma and malt lymphoma. the pathogenicity induced by different hp strains are associated to the functions of some hp genes and respective oligonucleotides, such as caga, vaca and icea. this study was designed to assemble these genes in gastric carcinoma and chronic gastritis biopsies. the hp dna was extracted from 39 formalin fixed paraffin embedded gastric samples, 26 from gastric carcinoma and 13 from chronic gastritis through 2 different methods: sections on glass slides and isolated sections cut to eppendorf tubes. the caga, vaca and icea genes were then detected by nested-pcr. the study of associations between the genotypes of the strains of h. pylori in gc and cg was found statistically significant differences for allele ice a1 (p < 0.05, or = 4.25). most of the gc with caga + vaca are associated with s2/m2 (46%, 12/26). the combination s2/m2/caga/icea1 predominantly occurred in 35% of cg compared with 30% of gc (p > 0.05, or = 1.19). for combinations s2/m2/caga/icea2 and s1/m2/caga/ice2 obtained a value of 12% and 30% for gc and gc (p> 0.05, or = 0.29). this knowledge is important for continuity of further studies aiming to define the characteristics of h. pylori genotype in pre-neoplasic lesions of the stomach.
Genotipagem do Helicobacter pylori no Carcinoma Gástrico e Gastrite Crónica Genotypes of Helicobacter pylori in Gastric Cancer and Chronic Gastritis
Maria Reis Silva,Carla Oliveira,Paulo Rodrigues-Santos,Lina Carvalho
Jornal Português de Gastrenterologia , 2011,
Abstract: A infec o gástrica provocada pelo Helicobacter pylori (Hp) é a principal causa da gastrite crónica do tipo B e condi o pré neoplásica para o desenvolvimento de carcinoma gástrico e linfoma MALT. A patogenicidade provocada por diferentes estirpes do Hp está associada à fun o de alguns genes desta bactéria e respectivos oligonucleotídeos, como o cagA, vacA e iceA. Pretendeu-se pesquisar a presen a destes genes em produtos de biopsia de carcinoma gástrico e gastrite crónica. O DNA bacteriano foi extraído de 39 amostras fixadas em formol e incluídas em parafina, das quais 26 correspondiam a carcinoma gástrico e 13 a gastrite crónica, por 2 técnicas distintas: sec es das biopsias sobre laminas e colhidas para tubo com xilol. Os genes cagA, vacA e iceA foram depois pesquisados através de nested-PCR. Do estudo de associa es entre os genótipos das estripes de H. pylori em CG e GC encontrou-se diferen as estatisticamente significativas para o alelo ice A1 (p < 0,05; OR = 4,25). A maioria dos GC com cagA+ est o associadas ao vacA s2/m2 (46%,12/26). A combina o s2/m2/cagA/iceA1 ocorreu predominantemente em 35% das CG comparado com 30% dos GC, (P > 0.05; OR = 1,19). Para as combina es s2/m2/cagA/iceA2 e s1/m2/cagA/ice2 obteve-se um valor de 12% para CG e 30% para GC, (P > 0,05; OR = 0,29). Estes conhecimentos s o importantes para continuidade de outros estudos que pretendam determinar as características do genótipo de H. pylori nas les es pré-neoplásicas do est mago. Helicobacter pylori (Hp) infection is the major cause of chronic active gastritis type B and a pre-neoplasic condition to the development of gastric carcinoma and MALT lymphoma. The pathogenicity induced by different Hp strains are associated to the functions of some Hp genes and respective oligonucleotides, such as cagA, vacA and iceA. This study was designed to assemble these genes in gastric carcinoma and chronic gastritis biopsies. The Hp DNA was extracted from 39 formalin fixed paraffin embedded gastric samples, 26 from gastric carcinoma and 13 from chronic gastritis through 2 different methods: sections on glass slides and isolated sections cut to eppendorf tubes. The cagA, vacA and iceA genes were then detected by nested-PCR. The study of associations between the genotypes of the strains of H. pylori in GC and CG was found statistically significant differences for allele ice A1 (p < 0.05, OR = 4.25). Most of the GC with cagA + vacA are associated with s2/m2 (46%, 12/26). The combination s2/m2/cagA/iceA1 predominantly occurred in 35% of CG compared with 30% of GC (P > 0.05, OR = 1.19). For
Preventive but Not Curative Efficacy of Celecoxib on Bladder Carcinogenesis in a Rat Model
José Sereno,Belmiro Parada,Flávio Reis,Fernanda X. Cunha,Edite Teixeira-Lemos,Patrícia Garrido,Rui Pinto,Petronila Rocha-Pereira,Paula Neto,José Ruivo,Paulo Rodrigues-Santos,Sara Nunes,Alfredo Mota,Arnaldo Figueiredo,Frederico Teixeira
Mediators of Inflammation , 2010, DOI: 10.1155/2010/380937
Abstract: To evaluate the effect of a cyclooxygenase 2 inhibitor, celecoxib (CEL), on bladder cancer inhibition in a rat model, when used as preventive versus as curative treatment. The study comprised 52 male Wistar rats, divided in 5 groups, during a 20-week protocol: control: vehicle, carcinogen: 0.05% of N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN), CEL: 10?mg/kg/day of the selective COX-2 inhibitor Celebrex, preventive CEL (CEL+BBN-P), and curative CEL (BBN+CEL-C) groups. Although tumor growth was markedly inhibited by the preventive application of CEL, it was even aggravated by the curative treatment. The incidence of gross bladder carcinoma was: control 0/8(0%), BBN 13/20(65%), CEL 0/8(0%), CEL+BBN-P 1/8(12.5%), and BBN+CEL-C 6/8(75%). The number and volume of carcinomas were significantly lower in the CEL+BBN-P versus BBN, accompanied by an ample reduction in hyperplasia, dysplasia, and papillary tumors as well as COX-2 immunostaining. In spite of the reduction of tumor volumes in the curative BBN+CEL-C group, tumor malignancy was augmented. An anti-inflammatory and antioxidant profile was encountered only in the group under preventive treatment. In conclusion, preventive, but not curative, celecoxib treatment promoted a striking inhibitory effect on bladder cancer development, reinforcing the potential role of chemopreventive strategies based on cyclooxygenase 2 inhibition. 1. Introduction Bladder cancer is a prevalent tumor, accounting for 5%–10% of all malignancies in Western countries [1, 2]. It has a high recurrence and progression rate and the prognosis, except for superficial forms, is poor [3]. Furthermore, it has high mortality rates and socioeconomic costs [4, 5]. The conventional surgical techniques and therapeutic options might cause discomfort to patients, especially in invasive and aggressive forms of cancer [6, 7]. Therefore, the improvement of bladder cancer management and treatment could rely on better preventive strategies. The identification of promising drugs remains dependent on a better elucidation of the molecular/cellular mechanisms underlying cancer appearance and progression [8, 9]. Apart from the genetic features and markers already characterized [10, 11], the cellular and molecular mechanisms for development and/or progression might involve inflammatory, proliferative, and oxidative stress phenomena that should be better elucidated. Inflammation through the cyclooxygenase (COX) pathway has been involved in cancer development [12]. While COX-1 is constitutively expressed in a huge range of tissues, playing a role in
Solvent-Free Synthesis, DNA-Topoisomerase II Activity and Molecular Docking Study of New Asymmetrically N,N'-Substituted Ureas
Andressa Esteves-Souza,Claudio E. Rodrigues-Santos,Catarina de Nigris Del Cistia,Daniel Rosa da Silva,Carlos Maurício R. Sant'Anna,Aurea Echevarria
Molecules , 2012, DOI: 10.3390/molecules171112882
Abstract: A new series of asymmetrically N,N'-substituted ureas 20–25 was prepared using solvent free conditions, which is an eco-friendly methodology, starting with Schiff bases derived from cinnamaldehyde and p-substituted anilines, which are subsequently submitted to reduction reactions that afford the corresponding asymmetric secondary amines. All of the intermediates were prepared using solvent free reactions, which were compared to traditional methodologies. All of the reactions required a remarkably short amount of time and provided good yields when solvent free conditions were employed compared to other methodologies. The DNA-topoisomerase II-α (topo II-α) activity was evaluated in relaxation assays, which showed that all of the compounds inhibited the enzyme activity at 10 μM, except for urea 24. Furthermore, a molecular docking study indicated that the compounds 20–25 binding to the topo II-α are able to interact with the same binding site as the anticancer drug etoposide, suggesting that the ureas could inhibit the enzyme by the same mechanism of action observed for etoposide, which prevents re-ligation of the DNA strands.
Avalia??o do possível efeito tóxico de um alcano semifluorinado de uso oftalmológico sobre cultura de células Vero
Estacia, Paulo;Santos Jr., Arnaldo Rodrigues;Genari, Selma Candelária;
Arquivos Brasileiros de Oftalmologia , 2004, DOI: 10.1590/S0004-27492004000600012
Abstract: purpose: perfluorocarbon liquids (pfcls) are used in vitreoretinal surgery. pfcls may cause inflammatory reactions, cellular injury and destruction of the normal retinal architecture. in order to avoid these effects, semifluorinated alkanes (sfa) were developed. we assessed the potential use of an sfa known as perfluorohexiloctano (f6h8) as long-term vitreous replacement under controlled cell culture conditions. methods: we analyzed indirect cytotoxicity, where the cells only come into contact with soluble elements that can be eliminated by perfluorohexiloctano. we therefore analyzed direct toxicity (contact toxicity) of perfluorohexiloctano by means of scanning electronic microscopy and immunocytochemistry reagents for actin. cells embedded in a treatment-free culture medium were used as control, a positive control for toxicity with an undeniably toxic effect on cells, and a weight control that produced a mechanical compression similar to the amount of perfluorohexiloctano used in the experiment. results: the indirect cytotoxicity test showed that f6h8 did not affect cell growth. our direct toxicity tests showed that cellular alterations caused by perfluorohexiloctano were similar to those produced by the weight control and different from toxicity control. conclusion: perfluorohexiloctano does not present indirect toxicity and this product has a compressive rather than a toxic effect on cultured cells.
Varia??o somaclonal em mudas micropropagadas de bananeira, cultivar Pacovan
Santos, Cynthia Christina Carvalho dos;Rodrigues, Paulo Hercílio Viegas;
Bragantia , 2004, DOI: 10.1590/S0006-87052004000200005
Abstract: the occurrence of somaclonal variation for plants in developed from tissue culture is well documented in the literature. the present study evaluated the influence of numbers subculture in the induction of variants in pacovan banana tree (musa spp., aab group). apex stems were introduced and multiplied in vitro using culture media ms, with addition of 2.5 mg.l-1 of bap. in subsequent subcultures, ms with 4.0 mg.l-1 of bap was used to induce side buds. results showed that plants were regenerated with different numbers of subculture (3, 4, 5, 6, 7, 8, and 9). these data were compared with seedlings from developed rhizomes. all the plants, were observed at field conditions in vale do a?u, rio grande do norte state. the percentage of somaclonal variants, some was estimated using morphological characteristics, such as plant size, color and shape of leaves and bunch shape. only plants in which buds showed the same type of variation were considered as variables. somaclonal variation occurred from the fifth subculture, and on the 9th subculture a 5.8% variation was observed. the increase of percentage of somaclonal variation, due to a higher number of subcultures, indicates the necessity of protocols for micropropagation specific for each variety to be commercialized.
A Escola de Minas de Ouro Preto, a "Sociedade de Geographia Economica de Minas Geraes" e as Exposi??es Universais do final do século XIX e início do século XX
Santos, Paulo Coelho Mesquita;Costa, Adilson Rodrigues da;
Rem: Revista Escola de Minas , 2005, DOI: 10.1590/S0370-44672005000300014
Abstract: this article assembles information obtained from the documental library of the escola de minas de ouro preto (school of mines, ouro preto). this paper systematically analyzes these documents. this study was begun a little more than a year ago and belongs to the project "organiza??o, restaura??o e difus?o do acervo técnicao científico da ufop: novos elementos para história de ciência no brasil" (organization, restauration and disclosure of the technical scientific library at ufop: new elements of brazil's scientific history) sponsered by cnpq. as can be inferred from the title of this article, the proposal is to go back to universal expositions as catalystic events of programed actions with the disclosure of conquests attributed to technical scientific progress. the work coordinated and performed by the escola de minas de ouro preto (emop), both in the province and later in the state of minas gerais, will be written with emphasis on the "sociedade de geographia econ?mic de minas geraes" (the minas gerais society of geographic economy).
A Escola de Minas de Ouro Preto e as "Se??es de Geologia" do Brasil nas Exposi??es Universais
Santos, Paulo Coelho Mesquita;Costa, Adilson Rodrigues da;
Rem: Revista Escola de Minas , 2006, DOI: 10.1590/S0370-44672006000300016
Abstract: this article is in accordance with several activities developed under the project "organiza??o, restaura??o e difus?o do acervo técnico científico da ufop: novos elementos para a história da ciência no brasil". initially, antique scientific equipment was cleaned and restored. found then, were documents revealing unknown features of ouro preto school of mines, its members and former students. these documents provide a better understanding of the role played by opsm in the organization of the "geology sections" where the state of the art of our mining industry and research activities were displayed at the universal expositions.
A escola diante das culturas juvenis: reconhecer para dialogar
Carlos Henrique dos Santos Martins,Paulo Cesar Rodrigues Carrano
Educa??o : Revista do Centro de Educa??o UFSM , 2011, DOI: 10.5902/198464442910
Abstract: Diversas manifesta es culturais presentes na cidade – e com baixa visibilidade no espa o escolar – têm os jovens como atores principais. Os jovens criam espa os próprios de socializa o que se transformam em territórios culturalmente expressivos e nos quais diferentes identidades s o elaboradas. A cultura se manifesta como espa o social privilegiado de práticas, representa es, símbolos e rituais. A produ o das identidades, além de demarcar territórios de sociabilidades e de práticas coletivas, p e em jogo interesses em comum que d o sentido ao “estar junto” e ao ser dos grupos. Nos territórios culturais juvenis delineam-se espa os de autonomia conquistados pelos jovens e que permitem a eles e elas transformar esses mesmos ambientes ressignificando-os a partir de suas práticas específicas. O artigo apresenta e discute processos sociais e culturais contemporaneos produtores das denominadas culturas juvenis e procura chamar a aten o para o necessário reconhecimento desses processos pela escola. Os grupos culturais juvenis s o decisivos na socializa o dos jovens que frequentam a escola de Ensino Médio que, além de alunos, s o, também, sujeitos de outros espa os e tempos culturais da cidade. Palavras-chave: Juventude; Culturas juvenis; Ensino Médio.
Um estudo comparativo sobre a resistência e o enfraquecimento do Grupo Católico em duas regi es brasileiras = A comparative study on the resistance and weakening of the Catholic Group in two Brazilian regions
Paulo Gracino Júnior,Denise dos Santos Rodrigues
Acta Scientiarum : Human and Social Sciences , 2008,
Abstract: O artigo estudo comparativo sobre a resistência e o enfraquecimento do Grupo Católico em duas regi es brasileiras aborda a diversidade religiosa de duas regi es dos Estados brasileiros do Rio de Janeiro e de Minas Gerais, destacando a rela o entre taxas de mobilidade populacional e pluralismo religioso. O acompanhamento de literaturaespecializada indica que os maiores índices de diversidade concentram-se nas regi es de intenso fluxo demográfico nas décadas recentes. Nas grandes cidades como Belo Horizonte e Rio de Janeiro, esses índices parecem puxados, sobretudo, pela migra o dos fiéis católicos para igrejas pentecostais ou para o grupo dos sem-religi o, enquanto nas regi es com baixo nível de mobilidade populacional, como o interior de Minas Gerais, ainda há focos de resistência católica. Com isso, verificamos que, onde há grande oferta religiosa, aumenta amigra o para outros grupos. Assim, apresentamos a regi o Metropolitana do Rio de Janeiro, com um dos maiores índices de diversidade religiosa do país, e a microrregi o de Ouro Preto em Minas Gerais, amplamente dominada pelo catolicismo, como casossignificativos para nossa argumenta o. The article A comparative study on the resistance and weakening of the Catholic Group in two Brazilian regions presents the religious diversity in two Brazilian areas – Rio de Janeiro and Minas Gerais – highlighting the relationship between the rates of population mobility and religious pluralism. Texts on Sociology of Religion indicate that the largest diversity indexes are concentrated in the areas that have presented intense demographic flow in the recent decades. In the larger cities such as Belo Horizonte and Rio de Janeiro, such indexes seem to be the result of the migration of theCatholic faithful to Pentecostal churches or to the group of people without religion, whereas in the areas with low level of populational mobility, such as those in the interior of Minas Gerais State, there are still representations of Catholic resistance. Thus, we concludethat the migration to other groups increases where there is great religious supply. The metropolitan area of Rio de Janeiro, where there are the largest indexes of religious diversity in the country, as well as in the area of Ouro Preto, in Minas Gerais State, thoroughly dominated by the Catholics, may be significant cases for our argument.
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