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Search Results: 1 - 10 of 427897 matches for " Paula Andrea M. Fonseca "
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Projecting Extreme Changes in Summer Rainfall in South America by the Middle of the 21st Century  [PDF]
Paula Andrea M. Fonseca, José Augusto P. Veiga, Francis Wagner S. Correia, Adriane L. Brito, M?nica R. Queiroz, André A. Lyra, Sin Chan Chou
Atmospheric and Climate Sciences (ACS) , 2014, DOI: 10.4236/acs.2014.44067
Abstract:

Extreme rainfall events can be considered a natural manifestation of the environment in which they are embedded and foreknowledge about their future behavior is very important, especially for decision makers. In this context, we aimed to explore the future behavior of extreme rainfall intensity through numerical simulations with the ETA model. The model was forced with a scenario of high greenhouse gas emissions for the middle of the 21st Century as described for A1B emission scenario. We detailed the main changes in accumulated rainfall produced by heavy events, very heavy events and rare events over a broad area of South America with a focus on the tropical sector. The methodology applied here is capable of separating extreme events and establishing the quantity of rainfall yielded by them. We have found that in the near future (2041-2050) rare events will tend to increase over the Amazon basin, followed by reductions in heavy and very heavy events. Conversely, heavy, very heavy and rare events are expected to decline over north-east Brazil. Furthermore, increases were found for heavy, very heavy and rare events over southern Brazil.

Subtyping of STEC by MLVA in Argentina
Andrea M. Sanso,Paula M. A. Lucchesi
Frontiers in Cellular and Infection Microbiology , 2012, DOI: 10.3389/fcimb.2012.00111
Abstract: Shiga toxin-producing Escherichia coli (STEC) causes serious human illness such as hemolytic uremic syndrome (HUS). Argentina has the world’s highest rate of this syndrome, which is the leading cause of acute renal failure among children. E. coli O157:H7 is the most common cause of HUS, but a substantial and growing proportion of this illness is caused by infection due to non-O157 strains. Multiple-locus variable-number tandem repeat analysis (MLVA) has become an established technique to subtype STEC. This review will address the use of routine STEC subtyping by MLVA in order to type this group of isolates and to get insight into the genetic diversity of native STEC. With regard to these objectives we modified and adapted two MLVA protocols, one exclusive for O157 and the other, a generic E. coli assay. A total of 202 STEC isolates, from different sources and corresponding to 20 serotypes, have been MLVA genotyped in our laboratory. In our experience, MLVA constitutes a very sensitive tool and enables us to perform an efficient STEC subtyping. The diversity found in many serotypes may be useful for future epidemiological studies of STEC clonality, applied to O157 as well as to non-O157 isolates.
O Escurecimento do Altar da Sé do Porto: Um Caso de Corros?o Atmosférica
Homem,Paula M.; Fonseca,Inês T. E.; Cavalheiro,José T.;
Corros?o e Protec??o de Materiais , 2008,
Abstract: samples taken from the different zones of the silver altar of porto?s cathedral were characterized by morphological and analytical techniques. it was concluded that the material used in the altar piece was ag:cu alloys with ag ranging between 62.9 % and 97.8 %. concerning to the corrosion products it was possible to postulate the existence of silver compounds, as chlorides, and copper compounds, as oxides and sulphates. other products identified related with the environment were the sodium and potassium sulphates and aluminosilicates, apart from some cleaning residues.
Ttraumatic bone cyst: report of a case diagnosed after orthodontic treatment
Motta, Andrea Fonseca Jardim da,Torres, Sandra Regina,Coutinho, Antonio Carlos de Azeredo,Basso, Ana Paula et al.
Revista Odonto Ciência , 2007,
Abstract: O cisto ósseo traumático é uma condi o incomum que pode ser diagnosticada ao acaso em tratamento odontológico de rotina. Os aspectos clínicos envolvem les o assintomática, sem expans o óssea, mais comumente localizada na regi o posterior da mandíbula, afetando preferencialmente indivíduos na segunda década de vida. Radiograficamente, o cisto ósseo traumático apresenta uma área radiolúcida bem definida, com padr o festonado ao redor dos ápices dos dentes adjacentes. As radiografias de rotina cumprem papel importante na identifica o da les o. A confirma o do diagnóstico é geralmente confirmada no ato cirúrgico, quando uma cavidade vazia é encontrada. Este relato de caso envolve uma situa o clínica em que o cisto ósseo traumático foi diagnosticado a partir da documenta o radiográfica ao final de um tratamento ortod ntico.
Profilaxis de infección por virus respiratorios en ni os y adultos sometidos a trasplante de órganos sólidos y precursores hematopoyéticos Prophylaxis against respiratory viral disease in pediatric and adult patients undergoing solid organ and hematopoietic stem cells transplantation
Ana M álvarez,Paula Catalán,Andrea Alba,Marcela Zubleta
Revista chilena de infectología , 2012,
Abstract: Los virus respiratorios se han identificado como causa de morbi-mortalidad en pacientes sometidos a TOS y TPH, particularmente en pediatría. Los más frecuentes son virus respiratorio sincicial (VRS), influenza (FLU), parainfluenza (PI) y adenovirus (ADV). La fuente de contagio está en la comunidad y en el hospital afectando al paciente en cualquier período post-trasplante. Se describe progresión a infecciones graves del tracto respiratorio bajo hasta en 60 % de los casos. Se recomienda aplicar medidas de aislamiento de precaución universal para todos los virus respiratorios (A2) y se describen algunas medidas específicas para FLU y AlDV. Vacunación anti-influenza anual con vacuna inactivada (en TOS a partir de 4-6 meses post-trasplante (A2), en TPH a partir de 6 meses (A2)); profilaxis post exposición a virus FLU (oseltamivir durante 10 días (B2)). En trasplante de pulmón, la duración de la profilaxis se extenderá mientras dure el período de riesgo (B2). Con respecto a ADV, no se dispone de una vacuna adecuada y no existe a la fecha una estrategia validada de quimioprofilaxis para prevenir enfermedad por ADV; en casos específicos de TPH pediátrico, se recomienda vigilancia semanal con RPC en sangre periférica hasta el día +100 post-TPH (A3). Respiratory viruses have been identified as a cause of morbidity and mortality in patients undergoing SOT and HSCT, specially in children. The most frequent are respiratory syncytial virus (RSV), influenza (FLU), parainfluenza (PI) and adenovirus (ADV). These infections are associated with progression to severe lower respiratory tract infections in up to 60% of the cases. It is advised to apply universal protection recommendations for respiratory viruses (A2) and some specific measures for FLU and AD. FLU: Annual anti-influenza vaccination (from 4-6 months post-transplantation in SOT, 6 months in HSCT (A2)); post- exposure prophylaxis in FLU (oseltamivir for 10 days (B2)). In lung transplantion, the prophylaxis should last as long as the risk period (B2). ADV: There is no vaccine nor valid chemoprophylaxis strategy to prevent ADV disease. In some specific HSCT recipients, weekly PCR monitoring is recommended until day+100 (A3).
ACOMPA AMIENTO EN SALA DE PARTOS: REGLA O EXCEPCIóN
Andrea Cantero,Lucrecia Fiuri,Karina Furfaro,M. Paula Jankovic
Revista del Hospital Materno Infantil Ramón Sardá , 2010,
Abstract: El apoyo continuo durante el trabajo de parto y parto se ha convertido en una excepción en vez de brindarse en forma habitual. Objetivos. a) Primario: Determinar en qué porcentaje las mujeres ingresan a sala de partos acompa adas por una persona de su elección. b) Secundarios: Identificar las causas que impiden el ingreso del acompa ante a sala de partos. Determinar el número de embarazadas que realizan el curso de preparación integral para la maternidad. Material y métodos. Dise o observacional, transversal; se realizó una encuesta a 390 gestantes que ingresaron al Hospital Materno Infantil Ramón Sardá para resolución de su parto, desde diciembre del 2007 hasta febrero del 2008 inclusive. Las variables fueron: deseo de estar acompa ada en el parto, ingreso de acompa ante, causa del no ingreso del acompa ante y asistencia al curso de preparación integral para la maternidad. Análisis estadístico. Frecuencias y sus IC 95%. Resultados. El 82,82%, deseó estar en compa ía de algún familiar. De las que desearon estar acompa adas, el 53,56% logró ese objetivo y el resto ingresaron a sala de partos sin acompa ante. Sólo el 10,76% había realizado el curso de preparación integral para la maternidad. Conclusiones. Existe un fuerte compromiso en llegar a ser una Maternidad Centrada en la Familia, pero todavía existen importantes interferencias que atentan para lograr este objetivo.
Evaluation of immunogenicity elicited from two DNA vaccine candidates that expresses the prM and E genes of the dengue-3 virus  [PDF]
Sérgio O. de Paula, Rafael F. O. Fran?a, Danielle M. Lima, Nina R. Dutra, Marília B. de Paula, Michelle D. de Oliveira, Leandro L. de Oliveira, Benedito A. L. da Fonseca
Health (Health) , 2010, DOI: 10.4236/health.2010.211193
Abstract: In this work, we report the evaluation of two DNA vaccines against dengue-3 virus (DENV-3). The first construction, called pVAC3DEN3, was engineered inserting the pre-membrane (prM) and envelope (E) gene of DENV-3 truncated with a restriction site between them, as previously described. The second construction was developed cloning the full gene sequence of prM and E from DENV-3 virus in pCI plasmid for mammalian expression and was denominated pVAC1WDEN3. The results showed that both constructions were capable of expressing the prM and E proteins, as demonstrated by ELISA and immunoblotting detection in cell culture transfected with the plasmids. After positive “in vitro” results, the vaccine candidates were used to immunize BALB/c mice and the elicited response was investigated. After immunization by intramuscular inoculation with three doses of each vaccinal clone the animals were sacrificed, the cytokine levels and T cell response were analyzed in the spleens, after three days of culture with stimulus, our analysis showed that the two constructions elicited T cell responses mea- sured by BrdU incorporation assay and high levels of IFN-γ, detected in the supernatant of the cultures. Moreover, both constructions induced detectable titers of neutralizing antibodies in mice. And finally the survival rate of the immunized animals after intracerebral challenge was analyzed, showing a better result in the pVAC3DEN3 group with an 80% survival compared with a 50% survival of the pVAC1 WDEN3. Thus, these data showed that our two constructions were able to induce specific immune response and protects mice against a lethal challenge with DENV-3, and these vaccine candidates can be employed to develop a viable dengue vaccine.
Erradica??o de Fusarium oxysporum em sementes de alfafa utilizando termo e quimioterapia
MENDES, MARTA. A. S.;LIMA, PAULA M. M. P.;FONSECA, JOSé N. L.;SANTOS, MARIA F.;
Fitopatologia Brasileira , 2001, DOI: 10.1590/S0100-41582001000200005
Abstract: the dry thermal treatment was conducted in a continuous air flux chamber on alfafa (medicago sativa) seeds with relative humidity under 5%. seeds underwent a pre-treatment for 60 °c during three or six hours, followed by a treatment at 90 °c for three or six hours. the wet treatment was conducted in water bath, and consisted of a pre-treatment at 40 °c for 10, 20 or 30 min, and then treatments at 50 °c or 60 °c for 10, 20 or 30 min. the chemicals tested were benomyl, thiabendazole, iprodione and thiram, at the dosages recommended by their manufactures and also at three times these dosages. the wet thermal treatment at 40 °c for 20 min, followed by the treatment at 50 °c for 20 min, were effective in the eradication of fusarium oxysporum, but significantly affected the alfafa seed germination. the dry thermal treatment at 60 °c for 3 h, followed by the treatment of 90 °c for 3 to 6 h, eradicated f. oxysporum without a significant reduction in seed germination. benomyl was the most efficient chemical product in eradicating f. oxysporum without affecting the alfalfa seed germination.
Erradica o de Fusarium oxysporum em sementes de alfafa utilizando termo e quimioterapia
MENDES MARTA. A. S.,LIMA PAULA M. M. P.,FONSECA JOSé N. L.,SANTOS MARIA F.
Fitopatologia Brasileira , 2001,
Abstract: Sementes de alfafa (Medicago sativa) contaminadas in vitro com Fusarium oxysporum foram submetidas a tratamento térmico seco, térmico úmido e químico, visando a erradica o deste patógeno. O tratamento térmico seco foi realizado em camara com fluxo de ar constante, em sementes com umidade relativa abaixo de 5%, pré-tratamento a 60 °C por 3 ou 6 h, seguido do tratamento a 90 °C por 3 ou 6 h. O tratamento térmico úmido foi realizado em banho-maria, com pré-tratamento a 40 °C por 10, 20 ou 30 min e tratamentos a 50 °C ou 60 °C por 10, 20 e 30 min. Os produtos químicos testados foram benomyl, thiabendazole, iprodione e thiram, nas dosagens recomendadas pelos fabricantes e o triplo dessas dosagens. Foram avaliados os efeitos dos tratamentos no controle de F. oxysporum, pelo método de "Blotter test", e na germina o das sementes. O tratamento térmico úmido, 40 °C por 20 min, seguido de 50 °C por 20 min, erradicou o patógeno F. oxysporum, mas reduziu significativamente a germina o das sementes. O tratamento térmico seco a 60 °C por 3 h, seguido de 90 oC por 3 ou 6 h de exposi o, erradicou F. oxysporum sem alterar significativamente a germina o das sementes. Benomyl foi o mais eficiente dos produtos químicos testados, erradicando F. oxysporum sem afetar a germina o das sementes de alfafa.
Desempenho e características de carca a de novilhos suplementados no período das águas
Zervoudakis Joanis Tilemahos,Paulino Mário Fonseca,Detmann Edenio,Lana Rogério de Paula
Revista Brasileira de Zootecnia , 2001,
Abstract: Avaliaram-se os efeitos da suplementa o concentrada de novilhos, durante o período das águas, sobre o desempenho e características da carca a. Foram utilizados 49 novilhos mesti os, mantidos em pastagem de Brachiaria decumbens. O experimento foi conduzido em três períodos experimentais, segundo um delineamento inteiramente casualizado. Foram fornecidos sal mineral (SAL), suplementos à base de milho e farelo de soja, com 20% PB, em níveis de 1,0 e 2,0 kg/dia (MFS1 e MFS2), e suplementos à base de farelo de trigo e farelo de soja, com 20% PB, em níveis de 1,0 e 2,0 kg/dia (FTFS1 e FTFS2), fornecidos diariamente. N o foram encontradas diferen as entre os tratamentos sobre ganho de peso médio diário (GMD) e ganho total (GDP Total). Os GMD foram: 0,887; 0,936; 1,047; 0,943; e 1,012 kg/animal/dia, respectivamente, para os tratamentos SAL, MFS1, MFS2, FTFS1 e FTFS2. Os animais submetidos aos diferentes tratamentos n o exibiram diferen as nas características físicas e químicas da carca a. N o houve diferen as quanto ao rendimento de carca a dos novilhos dos diferentes tratamentos, que foi, em média, de 52,19%.
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