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Search Results: 1 - 10 of 12356 matches for " Patrícia Penido "
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Diazepam e nordiazepam em plasma: métodos de extra o líquido-líquido e em fase sólida no pré-tratamento de amostras para análise cromatográfica em fase líquida
Freire Ellen Figueiredo,Miranda Juniella Luiza,Maia Patrícia Penido,Vieira Elisabeth Pizzamiglio
Química Nova , 2005,
Abstract: Analysis of diazepam (DZP) and its active metabolite nordiazepam (NDZP) in plasma is commonly performed in clinical medicine to ensure proper therapeutic effects while minimizing the incidence of toxicity. This study aimed to optimize analytical parameters and compare two pre-treatment techniques, liquid-liquid (LLE) and solid phase extraction (SPE), as well as liquid chromatographic conditions to analyze simultaneously DZP and NDZP in plasma from 20 patients treated with a daily dose of 10 mg. Both techniques showed to be well in line with the international criteria for analytical validation, which permitted to quantify DZP (66.2 - 1148.6 ng mL-1) and NDZP (138.5 - 808.6 ng mL -1) in all samples. The correlation coefficients between SPE and LLE were respectively 0.9729 for DZP and 0.9643 for NDZP.
Uso de gerenciador de conteúdos e hipertextos: gest o na biblioteca universitária
Benildes Coura Moreira dos Santos Maculan,Fernanda Pereira,Gercina Lima,Patrícia Penido
Revista ACB , 2011,
Abstract: Indica o papel da aplica o dos gestores de conteúdos e hipertextos na gest o de bibliotecas. Apresenta os sistemas de gerenciamento de conteúdo para criar websites, portais ou intranets. Aponta esses softwares, que integram elementos para a viabilidade de implanta o da gest o de informa es, e podem ter a vantagem de ser livre. Um dos fatores da gest o da informa o envolve identificar diferentes metodologias de representa o da informa o para o usuário, seja ele o usuário final ou o funcionário. A estrutura de links e conex es do hipertexto, criando inter-rela o entre conceitos, o que é fator crucial na recupera o da informa o.
Taxonomia facetada como interface para facilitar o acesso à informa o em bibilotecas digitais
Benildes Coura Moreira dos Santos Maculan,Gercina Angela Borém de Oliveira Lima,Patrícia Penido
Revista ACB , 2011,
Abstract: As bibliotecas digitais comumente oferecem interface de busca com duas formas: 1) busca simples; e 2) busca avan ada. Neste artigo pretende-se apresentar uma interface de busca usando taxonomia facetada. A classifica o vem sendo aplicada como forma de acomoda o de informa es visando à recupera o do conteúdo de sites, principalmente no e-commerce. O fator restringente nos sistemas de recupera o da informa o é o modo de representa o utilizado, que muitas vezes n o permite a transferência e comunicabilidade do conhecimento. Busca-se, com a taxonomia facetada como interface de busca, estruturar os dados de forma flexível, permitindo a op o de cruzamento de informa es.
QuEChERS-HPLC-DAD method for sulphonamides in chicken breast
Simone Caetani Machado,Mariane Landin-Silva,Patrícia Penido Maia,Susanne Rath
Brazilian Journal of Pharmaceutical Sciences , 2013,
Abstract: The development of a QuEChERS-HPLC-DAD method using a Lichrospher 60 RP-Select B column (250 x 4.6 mm x 5 μm) at 40oC, mobile phase constituted by phosphate buffer:acetonitrile (75:25, v/v) at a initial flow rate of 0.5 mL min-1, increased by 1.2 mL min-1 and at 265 nm is presented for simultaneous determination of sulphadiazine, sulphametoxipiridazine and sulphamethoxazole in chicken breast samples. QuEchERS is inexpensive, fast and easy, and the extraction of the analytes of the matrix was successfully employed. In addition, the method presented linearity, in the range of 25, 50, 100, 150, 175, and 200 μg kg-1, precision, selectivity and sensitivity. The intraday precision (RSD %) for QuEChERS method was between 3.6-10.8 (SDZ), 6.9-14.1 (SPZ) and 1.9-10.9 (SMX) and interday precision (RSD%) was between 1.5-9.7, 1.7-4.1 and 2.1-10.2, respectively. Results of accuracy (bias) were in the range of -8.6 to +11.9 %. Therefore, the validated method is clearly useful for the practical residue monitoring of the drugs evaluated in chicken samples, as all the values were within the acceptable criteria used for food safety. Of 6 samples analyzed, none of them showed contamination of the sulphonamides studied at detectable levels. O desenvolvimento de um método QuEChERS-HPLC-DAD usando uma coluna Lichrospher RP-60 Select B (250 x 4,6 mm x 5 μm) a 40 oC, fase móvel constituída por tamp o de fosfato: acetonitrila (75:25, v/v) a uma vaz o inicial de 0,5 mL min-1, aumentando 1,2 mL min-1 e a 265 nm é apresentado para a determina o simultanea de sulfadiazina, sulfametoxipiridazina e sulfametoxazol em amostras de peito de frango. O QuEChERS é barato, rápido e fácil, e a extra o dos analitos da matriz foi empregada com sucesso. Além disso, o método apresentou linearidade, na faixa de 25, 50, 100, 150, 175 e 200 μg kg-1, precis o, seletividade e sensibilidade. A precis o intradia (RSD %) para o método QuEChERS foi entre 3,6-10,8 (SDZ), 6,9-14,1 (SPZ) e 1,9-10,9 (SMX) e a precis o interdias (RSD%) foi entre 1,5-9,7, 1,7-4,1 e 2,1-10,1, respectivamente. Resultados de exatid o (tendenciosidade) foram na faixa de -8,6 a +11,9%. Portanto, o método validado é útil para a monitoriza o de resíduos de medicamentos avaliados em amostras de frangos, bem como todos os valores estavam dentro dos critérios aceitáveis utilizados para a seguran a dos alimentos. De seis amostras analisadas, nenhuma apresentou contamina o de sulfonamidas nos níveis detectáveis estudados.
Single gas chromatography method with nitrogen phosphorus detector for urinary cotinine determination in passive and active smokers
Malafatti, Lusiane;Maia, Patrícia Penido;Martins, Matheus Coutinho Gon?alves;Siqueira, Maria Elisa Pereira Bastos de;Martins, Isarita;
Brazilian Journal of Pharmaceutical Sciences , 2010, DOI: 10.1590/S1984-82502010000400019
Abstract: nicotine is a major addictive compound in cigarettes and is rapidly and extensively metabolized to several metabolites in humans, including urinary cotinine, considered a biomarker due to its high concentration compared to other metabolites. the aim of this study was to develop a single method for determination of urinary cotinine, in active and passive smokers, by gas chromatography with a nitrogen phosphorus detector (gc-npd). urine (5.0 ml) was extracted with 1.0 ml of sodium hydroxide 5 mol l-1, 5.0 ml of chloroform, and lidocaine used as the internal standard. injection volume was 1 μl in gc-npd. limit of quantification was 10 ng ml-1. linearity was evaluated in the ranges 10-1000 ng ml-1 and 500-6000 ng ml-1, with determination coefficients of 0.9986 and 0.9952, respectively. intra- and inter-assay standard relative deviations were lower than 14.2 %, while inaccuracy (bias) was less than +11.9%. the efficiency of extraction was greater than 88.5%. ruggedness was verified, according to youden's test. means of cotinine concentrations observed were 2,980 ng ml-1 for active smokers and 132 ng ml-1, for passive smokers. the results revealed that satisfactory chromatographic separation between the analyte and interferents was obtained with a zb-1 column. this method is reliable, precise, linear and presented ruggedness in the range evaluated. the results suggest that it can be applied in routine analysis for passive and active smokers, since it is able to quantify a wide range of cotinine concentrations in urine.
Soja transgênica BRS 243 RR: determina??o de macronutrientes e das isoflavonas daidzeína e genisteína por Cromatografia Líquida de Alta Eficiência (CLAE)
Alezandro, Marcela Roquim;Almeida, Sandra Aparecida de;Maia, Patrícia Penido;Carvalho, Helenice Aparecida de;Azevedo, Luciana;Vieira, Elisabeth Pizzamiglio;
Ciência e Tecnologia de Alimentos , 2008, DOI: 10.1590/S0101-20612008000300003
Abstract: the objective of this work was to evaluate the proximate composition, as well as daidzein (d) and genistein (g) contents by hplc, of brs 243 rr soybean. sample preparation for the chromatographic analysis involved the use of hexane to remove lipids. isoflavones were extracted with 70% ethanol containing 0.1% acetic acid. the optimized chromatographic conditions were: c18 column, mobile phase methanol:5% acetic acid (1:1 v/v), flow rate 0.5 ml/minute, column temperature 30 °c, uv absorbance at 254 nm and volume injected 40 μl. the validation parameters were: linearity of daidzein (y = 11242 x -37433, r = 0.9976) and genistein (y = 18510 x -66761, r = 0.9980); variation coefficients obtained in intra-day precision assays [cv = 5.3% (d), cv = 6.7% (g)] and inter-day precision assays [cv = 8.7% (d), cv = 9.7% (g)]; quantification limit 10 μg.g-1; detection limit 5 μg.g-1 and recovery 95.7%. therefore, the optimized method was appropriate for the determination of daidzein and genistein in soybean. carbohydrate (31.4%), protein (35.9%), lipid (20.9%), moisture (6.9%), ash (4.9%), daidzein (44.1 μg.g-1) and genistein (37.4 μg.g-1) contents determined in transgenic soybean were similar to those of conventional soybean determined in other studies.
A role for lymphocytes and cytokines on the eosinophil migration induced by LPS
Castro-Faria-Neto, Hugo C;Penido, Carmen M;Larangeira, Andréa P;Silva, Adriana R;Bozza, Patrícia T;
Memórias do Instituto Oswaldo Cruz , 1997, DOI: 10.1590/S0074-02761997000800026
Abstract: in the present work we review the existing evidence for a lps-induced cytokine-mediated eosinophil accumulation in a model of acute inflammation. intrathoracic administration of lps into rodents (mice, rats or guinea pigs) induces a significant increase in the number of eosinophils recovered from the pleural fluid 24 hr later. this phenomenon is preceded by a neutrophil influx and accompanied by lymphocyte and monocyte accumulation. the eosinophil accumulation induced by lps is not affected by inhibitors of cyclo or lipoxygenase nor by paf antagonists but can be blocked by dexamethasone or the protein synthesis inhibitor cycloheximide. transfer of cell-free pleural wash from lps injected rats (lps-pw) to naive recipient animals induces a selective eosinophil accumulation within 24 hr. the eosinophilotactic activity present on the lps-pw has a molecular weight ranging between 10 and 50 kda and its effect is abolished by trypsin digestion of the pleural wash indicating the proteic nature of this activity. the production of the eosinophilotactic activity depends on the interaction between macrophages and t-lymphocytes and its effect can not be blocked by anti-il-5 monoclonal antibodies. accumulated evidence suggest that the eosinophil accumulation induced by lps is a consequence of a eosinophilotactic cytokine produced through macrophage and t-cell interactions in the site of a lps-induced inflammatory reaction.
Catheter-related infections in a northwestern S?o Paulo reference unit for burned patients care
Campos Júnior, Cláudio Penido;Sanches, Patrícia;Tedokon, Elizabete Aparecida;Souza, Ana Carolina Remondi;Machado, Ricardo Luiz Dantas;Rossit, Andréa Regina Baptista;
Brazilian Journal of Infectious Diseases , 2010, DOI: 10.1590/S1413-86702010000200009
Abstract: despite improvements in care and rehabilitation of burned patients, infections still remain the main complication and death cause. catheter-related infections are among the four most common infections and are associated with skin damage and insertion site colonization. there are few studies evaluating this kind of infection worldwide in this special group of patients. padre albino hospital burn care unit (pahbcu) is the only reference center in the northwestern s?o paulo for treatment of burned patients. this paper presents the results of a retrospective study aiming at describing the epidemiological and clinical features of catheter-related infections at pahbcu.
Liquid-liquid extraction combined with high performance liquid chromatography-diode array-ultra-violet for simultaneous determination of antineoplastic drugs in plasma
Sanson, Ananda Lima;Silva, Suéllen Cristina Rennó;Martins, Matheus Coutinho Gon?alves;Giusti-Paiva, Alexandre;Maia, Patrícia Penido;Martins, Isarita;
Brazilian Journal of Pharmaceutical Sciences , 2011, DOI: 10.1590/S1984-82502011000200017
Abstract: a liquid-liquid extraction (lle) combined with high-performance liquid chromatography-diode array detection method for simultaneous analysis of four chemically and structurally different antineoplastic drugs (cyclophosphamide, doxorubicin, 5-fluorouracil and ifosfamide) was developed. the assay was performed by isocratic elution, with a c18 column (5 μm, 250 x 4.6 mm) and mobile phase constituted by water ph 4.0- acetonitrile-methanol (68:19:13, v/v/v), which allowed satisfactory separation of the compounds of interest. lle, with ethyl acetate, was used for sample clean-up with recoveries ranging from 60 to 98%. the linear ranges were from 0.5 to 100 μg ml-1, for doxorubicin and 1 to 100 μg ml-1, for the other compounds. the relative standard deviations ranged from 5.5 to 17.7%. this method is a fast and simple alternative that can be used, simultaneously, for the determination of the four drugs in plasma, with a range enabling quantification of the drugs in pharmacokinetics, bioequivalence and therapeutic drug-monitoring studies.
Diazepam e nordiazepam em plasma: métodos de extra??o líquido-líquido e em fase sólida no pré-tratamento de amostras para análise cromatográfica em fase líquida
Freire, Ellen Figueiredo;Miranda, Juniella Luiza;Maia, Patrícia Penido;Vieira, Elisabeth Pizzamiglio;Borges, Keyller Bastos;Siqueira, Maria Elisa Pereira Bastos de;
Química Nova , 2005, DOI: 10.1590/S0100-40422005000500007
Abstract: analysis of diazepam (dzp) and its active metabolite nordiazepam (ndzp) in plasma is commonly performed in clinical medicine to ensure proper therapeutic effects while minimizing the incidence of toxicity. this study aimed to optimize analytical parameters and compare two pre-treatment techniques, liquid-liquid (lle) and solid phase extraction (spe), as well as liquid chromatographic conditions to analyze simultaneously dzp and ndzp in plasma from 20 patients treated with a daily dose of 10 mg. both techniques showed to be well in line with the international criteria for analytical validation, which permitted to quantify dzp (66.2 - 1148.6 ng ml-1) and ndzp (138.5 - 808.6 ng ml -1) in all samples. the correlation coefficients between spe and lle were respectively 0.9729 for dzp and 0.9643 for ndzp.
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