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Search Results: 1 - 10 of 300659 matches for " Patel Dhara J "
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Simultaneous estimation of amitriptyline HCl and perphenazine in tablets by UV-Visible spectrophotometric and HPTLC
Dhara J Patel,Vivek Patel
International Journal of Pharmacy and Biomedical Sciences , 2010,
Abstract: A simple and specific UV/Visible spectrophotometric and HPTLC methods were developed for the simultaneous determination of amitriptyline HCl and perphenazine in tablets. The former is the UV/Visible spectrophotometric method which involves the determination of amitriptyline HCl and perphenazine using the simultaneous equation method at 240.0 and 258.0 nm over the concentration ranges of 2-12 μg/mL for both. The later is the HPTLC method, where the separation of drugs was carried out on Merck thin layer chromatographic aluminium sheets of silica gel 60 F254 using the mobile phase containing carbon tetrachloride: toluene:methanol:triethyl amine (4:3:2:0.2, v/v/v). The separation was followed by densitometric measurements of their spots at 240 nm. The methods were validated for linearity precision, sensitivity, and specificity. The linearity was found to be in the range of 50–600 and 40–480 ng/spot for amitriptyline hydrochloride and perphenazine, respectively. The methods were specific because no chromatographic interferences from the tablet excipients were found and therefore, the proposed methods were successfully applied for the routine quantitative analysis of tablets containing amitriptyline HCl and perphenazine.
SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF MONTELUKAST SODIUM AND BAMBUTEROL HYDROCHLORIDE IN TABLETS
Patel Satish A,Patel Dhara J,Patel Natavarlal J.
International Research Journal of Pharmacy , 2011,
Abstract: The present manuscript describe simple, sensitive, rapid, accurate, precise and economical first derivative spectrophotometric method for the simultaneous determination of montelukast sodium and bambuterol hydrochloride in combined tablet dosage form. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first order derivative spectra was obtained in chloroform and the determinations were made at 241 nm (ZCP of bambuterol hydrochloride) for montelukast sodium and 258.4 nm (ZCP of montelukast sodium) for bambuterol hydrochloride. The linearity was obtained in the concentration range of 10-60 μg/ml for montelukast sodium and 10-80 μg/ml for bambuterol hydrochloride. The mean recovery was 100.1 ± 1.25 and 99.70 ± 1.38 for montelukast sodium and bambuterol hydrochloride, respectively. The method was found to be simple, sensitive, accurate and precise and was applicable for the simultaneous determination of montelukast sodium and bambuterol hydrochloride in pharmaceutical tablet dosage form. The results of analysis have been validated statistically and by recovery studies.
High Throughput Screening for Small Molecule Enhancers of the Interferon Signaling Pathway to Drive Next-Generation Antiviral Drug Discovery
Dhara A. Patel, Anand C. Patel, William C. Nolan, Yong Zhang, Michael J. Holtzman
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0036594
Abstract: Most of current strategies for antiviral therapeutics target the virus specifically and directly, but an alternative approach to drug discovery might be to enhance the immune response to a broad range of viruses. Based on clinical observation in humans and successful genetic strategies in experimental models, we reasoned that an improved interferon (IFN) signaling system might better protect against viral infection. Here we aimed to identify small molecular weight compounds that might mimic this beneficial effect and improve antiviral defense. Accordingly, we developed a cell-based high-throughput screening (HTS) assay to identify small molecules that enhance the IFN signaling pathway components. The assay is based on a phenotypic screen for increased IFN-stimulated response element (ISRE) activity in a fully automated and robust format (Z′>0.7). Application of this assay system to a library of 2240 compounds (including 2160 already approved or approvable drugs) led to the identification of 64 compounds with significant ISRE activity. From these, we chose the anthracycline antibiotic, idarubicin, for further validation and mechanism based on activity in the sub-μM range. We found that idarubicin action to increase ISRE activity was manifest by other members of this drug class and was independent of cytotoxic or topoisomerase inhibitory effects as well as endogenous IFN signaling or production. We also observed that this compound conferred a consequent increase in IFN-stimulated gene (ISG) expression and a significant antiviral effect using a similar dose-range in a cell-culture system inoculated with encephalomyocarditis virus (EMCV). The antiviral effect was also found at compound concentrations below the ones observed for cytotoxicity. Taken together, our results provide proof of concept for using activators of components of the IFN signaling pathway to improve IFN efficacy and antiviral immune defense as well as a validated HTS approach to identify small molecules that might achieve this therapeutic benefit.
Spectrum of Microbial Flora in Diabetic Foot Ulcer and Its Antibiotic Sensitivity Pattern in Tertiary Care Hospital in Ahmedabad, Gujarat
Manisha Jain,Mitesh H Patel,Nidhi K Sood,Dhara J Modi
National Journal of Medical Research , 2012,
Abstract: Introduction: A Prospective study “Spectrum of Microbial flora in diabetic foot ulcer and its antibiotic sensitivity pattern” was carried out in a tertiary care hospital, Ahmedabad on 125 patients in which 85 were male patients and 40 were female patients. Material and Methods: Swabs samples were collected from the edge and margins of ulcers and organism were identified by gram staining culture and biochemical reactions. Results: Out of 125 specimens 108 specimens showed growth of organisms. Total 157 aerobic organisms were isolated from culture positive specimens. It represents an average of 1.25 organisms per case. Among these organisms, 130 gram negative and 27 gram positive organisms were isolated. Pseudomonas aeruginosa (30.57%) was predominant organism followed by Klebsiella spp. (22.29%). Staphylococcus aureus were 12.74% in which Methicillin resistant S. aureus (MRSA) was 55%. Conclusion: incidence of growth was 86.4% in which Pseudomonas aeruginosa (30.57%) is most common isolate. Organisms in mixed infections showed multidrug resistance as compared to single isolated strain. Diabetic foot infections are polymicrobial in nature. As the Wagner’s grade increased, the prevalence of isolates also increased. [National J of Med Res 2012; 2(3.000): 354-357]
A Study of Extended Spectrum ?-Lactamase (ESBL) and AmpC ?-Lactamase Producing Klebsiella Pneumoniae in Neonatal Intensive Care Unit at Tertiary Care Hospital, Ahmedabad
Modi Dhara J, Patel Bhaumik V, Patel Mitesh H, Bhatt Seema S, Sood Nidhi K, Vegad Mahendra M
National Journal of Community Medicine , 2012,
Abstract: Background & objectives: Clinical laboratories need to develop quick screening methods for detection of Extended Spectrum ?-Lactamases (ESBL) & Amplified C (AmpC) ?-Lactamase, so that the appropriate medication can be started without delay. Here, we reported the screening & confirmatory methods for detection of ESBL & AmpC in Klebsiella pneumoniae in Neonatal Intensive Care Unit (NICU). Methods: We had tested 600 blood culture samples from the NICU patients. From the positive bacterial isolates, Klebsiella pneumoniae were screened for ESBL & AmpC production followed by confirmatory methods as per Clinical Laboratory Standard Institute (CLSI) guidelines. Results: Out of the 600 blood culture, 266 were positive for microbial growth. Among them, Klebsiella pneumoniae were in 54 patients. Out of 54 Klebsiella pneumoniae, 48 were positive for ESBL in screening method. Out of 48, 41 isolates were ESBL positive & 4 were AmpC positive by Confirmatory test (Modified Three Dimensional Method for AmpC). Interpretation & conclusions: The prevalence of ESBL & AmpC producing Klebsiella pneumoniae in NICU at our institute is 75.92% & 7.4% which is very alarming, and it requires strict implementation of infection control guidelines in NICU by safe hygiene practices, restricted use of broad spectrum antibiotics as empirical therapy in septicemic cases and also formulation of uniform antibiotic policy for such patients based on the current trend of antibiotic resistance. This can be helpful in preventing emergence of multidrug resistance in such organisms.
Formulation of Response Reduction Factor for RCC Framed Staging of Elevated Water Tank using Static Pushover Analysis
Bhavin Patel,Dhara Shah
Lecture Notes in Engineering and Computer Science , 2010,
Abstract:
Zinc and Chromium Load in Road Dust, Suspended Particulate Matter and Foliar Dust Deposits of Anand City, Gujarat  [PDF]
Tanushree Bhattacharya,Sukalyan Chakraborty,Dhara Tuteja,Mitul Patel
Open Journal of Metal (OJMetal) , 2013, DOI: 10.4236/ojmetal.2013.32a1006
Abstract: Anand, the milk capital of India, is a developing city with increasing vehicles and developmental activities going on at a fast pace. This study attempts to investigate the zinc and chromium concentration in street dust, suspended particulate matter and in foliar dust deposits. Ten sampling locations were selected based on the traffic density on the roads and different anthropogenic activity. Sampling was carried out in the dry months of January to March 2011. The range of Zn and Cr was 16.82 - 108.29 ppm and 118 - 151.5 ppm in the street dust respectively. Zn concentration in Suspended particulate matter lies in the range of 12.41 to 86 ppm and Cr concentration between 75 to 130 ppm. The range of Cr in foliar deposited dust varied from 79.54 ppm to 31 ppm. Whereas, for Zn maximum concentration was in S10 which is 42.34 ppm and minimum was in site S9, 23.73 ppm. ANOVA single factor showed that at 0.05 level of significance site wise variation of zinc and chromium concentration in SPM, Street dust and foliar deposited dust was not significant signifying similar source of contamination. Which is further strengthened by the good positive correlation found between the Zn and Cr concentration of street dust, leaf deposited dust and SPM. The Contamination Factor in the sites where metal concentration was high was 1.24 in S10 and 1.06 in S5 for Zn. For chromium the value of CF was 1.77 in S10 and 1.67 in S5. These values indicate that street dust is moderately contaminated with respect to zinc and chromium.
Zinc and Chromium Load in Road Dust, Suspended Particulate Matter and Foliar Dust Deposits of Anand City, Gujarat  [PDF]
Tanushree Bhattacharya, Sukalyan Chakraborty, Dhara Tuteja, Mitul Patel
Open Journal of Metal (OJMetal) , 2013, DOI: 10.4236/ojmetal.2013.32A1006
Abstract:

Anand, the milk capital of India, is a developing city with increasing vehicles and developmental activities going on at a fast pace. This study attempts to investigate the zinc and chromium concentration in street dust, suspended particulate matter and in foliar dust deposits. Ten sampling locations were selected based on the traffic density on the roads and different anthropogenic activity. Sampling was carried out in the dry months of January to March 2011. The range of Zn and Cr was 16.82 - 108.29 ppm and 118 - 151.5 ppm in the street dust respectively. Zn concentration in Suspended particulate matter lies in the range of 12.41 to 86 ppm and Cr concentration between 75 to 130 ppm. The range of Cr in foliar deposited dust varied from 79.54 ppm to 31 ppm. Whereas, for Zn maximum concentration was in S10 which is 42.34 ppm and minimum was in site S9, 23.73 ppm. ANOVA single factor showed that at 0.05 level of significance site wise variation of zinc and chromium concentration in SPM, Street dust and foliar deposited dust was not significant signifying similar source of contamination. Which is further strengthened by the good positive correlation found between the Zn and Cr concentration of street dust, leaf deposited dust and SPM. The Contamination Factor in the sites where metal concentration was high was 1.24 in S10 and 1.06 in S5 for Zn. For chromium the value of CF was 1.77 in S10 and 1.67 in S5. These values indicate that street dust is moderately contaminated with respect to zinc and chromium.

Development and Validation of a Stability-indicating HPTLC Method for Analysis of Antiasthmatic Drugs
Patel Nilam,Patel Shirish,Patel Dhara
International Journal For Pharmaceutical Research Scholars , 2012,
Abstract: The objective of the present work was to develop a stability-indicating HPTLC method for Montelukastsodium (MTKT) and Levocetirizine dihydrochloride (LCTZ) in the presence of its degradation productsgenerated from forced decomposition studies. Both drugs were subjected to acid, base, peroxide, andphoto degradation. Successful separation of the drugs from the degradation products formed onaluminum-backed silica gel 60 F254 with Ethyl acetate: Methanol: Ammonia (7.0:1.4:0.7 v/v/v) as themobile phase. Densitometric analysis of was performed at 231nm in concentration range 100-1200ng/spot with range of recovery 99.91± 0.91% for MTKT and 50-600 ng/spot with range of recovery99.28± 0.51 % for LCTZ by the HPTLC method. Statistical analysis proved the method to be repeatable,specific, and accurate for estimation of MTKT and LCTZ. It can be used as a stability indicating methoddue to its effective separation of the drugs from its degradation products.
Effect of low power microwave on microbial growth, enzyme activity, and aflatoxin production
Krunal Dholiya,Dhara Patel,Vijay Kothari
Research in Biotechnology , 2012,
Abstract: This study aimed at investigating the effect of low power (90 W) microwave radiation(2450 MHz) on microbial growth, enzyme activity (protease and urease), and aflatoxinproduction. Thermal effect was avoided by keeping inoculum in ice while treating withmicrowave (for varying duration i.e. 2, 4, and 6 min). After 6 min MW treatment to S. aureus,its growth was stimulated over control by ~10%. Total protease activity in Aeromonashydrophila witnessed a 33% decrease as compared to control after a microwave exposure of 2min. Similar decrease of 24% in total protease activity of Candida albicans was observed aftermicrowave exposure of 6 min. Staphylococcus aureus lost its urease activity completely aftermicrowave treatment. Aflatoxin production was completely inhibited in Aspergillusparasiticus after microwave exposure of 2, 4, and 6 min. However, it required a 6 minmicrowave exposure for complete inhibition of aflatoxin production in Aspergillus flavus.Our results positively suggest existence of microwave specific non-thermal effect onmicrobial growth and metabolism.
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