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BIOSURFACTANT AS A PESTICIDE CLEANING AGENT IN LEAFY VEGETABLES PRODUCED BY PSEUDOMONAS FLUORESCENS ISOLATED FROM MANGROVE ECOSYSTEM
GOVINDAMMAL M,PARTHASARATHI R
Golden Research Thoughts , 2013, DOI: 10.9780/22315063
Abstract: In this present investigation, biosurfactant was produced by Pseudomonas fluorescens MFS03 isolated from mangrove forest soil using groundnut oil cake as substrate. Characterization of the biosurfactant revealed that, it is a glycolipid with chemical composition of carbohydrate (0.868 mg/ml) and lipid (1.912 mg/ml). Based on the TLC analysis, carbohydrate and lipid with an Rf value of 0.72 were separated and it was partially identified as rhamnolipid. In FT-IR spectral analysis, important absorption bands at 3466.24, 2926.45, 1743.47, 1407.30 and 1162.26 cm-1 indicates the chemical structure is identical to those of rhamnolipids. Biosurfactant was used for the cleanup of insecticide residues in the spinach varieties Amaranthus tricolor. Lgenerally called as Arakkeerai or Killukeerai. The experiment was carried out under in-vitro conditions. Among the different washing solution (Salt, vinegar KMnO4 and biosurfactant) used for the reduction of pesticide residues, biosurfactant solution shows higher reduction. However the amount of biosurfactant and the contact times shows significant role in reducing cypermethrin residues in spinach. Cypermethrin solution with 100ppm concentration was reduced to below 2 ppm, which is safe level using 25 ppm of biosurfactant solution for 5 minutes. The increase in amount of biosurfactant concentration shows decrease in contact time in reducing cypermethrin residues. From this study we concluded that production of biosurfactant using groundnut oil cake is economically low-cost medium and eco-friendly and it can be used as an effective agent to clean up pesticide residues in vegetables and leafy vegetables.
BIOSURFACTANT PRODUCTION USING PINEAPPLE JUICE AS MEDIUM BY PSEUDOMONAS FLUORESCENS ISOLATED FROM MANGROVE FOREST SOIL.
GOVINDAMMAL M,PARTHASARATHI R
Indian Streams Research Journal , 2013,
Abstract: In the present investigation, Pineapple juice has been tested for its suitability for biosurfactant production using Pseudomonas fluorescens MFS03 isolated from the crude oil enriched mangrove soil. The strain recorded maximum biosurfactant production (9.43 g/l) when pineapple juice was used as a medium. Biosurfactant production was confirmed by conventional screening methods including hemolytic activity, drop collapsing test, oil displacement method, CTAB, lipase activity and emulsification index. The isolate shows lowest surface tension of 25.4 mN/m, an interfacial tension of 0.98 mN/m and a CMC of 34 mg/l. The active compound was extracted with ethyl acetate. The extract was fractionated by TLC to isolate the pure compound. Based on the TLC, chromatogram the biosurfactant was identified as glycolipid type. FT-IR spectrum revealed that the important adsorption bands at 3466.24, 2926.45, 1743.47, 1407.30 and 1162.26 cm-1 indicates the chemical structure of rhamnolipid. The results demonstrated that the Pineapple juice can be a suitable medium for the biosurfactant production, which can improve the process economical.
INVESTIGATION ON ANTIMICROBIAL ACTIVITY OF BIOSURFACTANT PRODUCED BY PSEUDOMONAS FLUORESCENS ISOLATED FROM MANGROVE ECOSYSTEM
Govindammal M,Parthasarathi R
International Research Journal of Pharmacy , 2013,
Abstract: The aim of this present study is to investigate the antimicrobial activity of rhamnolipid biosurfactant produced by Pseudomonas fluorescens MFS03 isolated from mangrove forest soil using groundnut oil cake as substrate. The biosurfactant was extracted with an equal amount of ethyl acetate and the concentrated extract was subjected to FT-IR analysis. The important adsorption bands at 3466.24, 2926.45, 1743.47, 1407.30 and 1162.26 cm-1indicate the chemical structure of rhamnolipid. The rhamnolipid biosurfactant was investigated for the potential antimicrobial activity by using disc-diffusion method against Gram positive bacteria (Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Methicillin resistance S. aureus) Gram negative bacteria (Escherichia coli, Salmonella typhimurium) and a yeast (Candida albicans). The biosurfactant showed distinct antibacterial activity towards tested bacteria and shows an antifungal activity against yeast. The biosurfactant with different concentration was performed for the evaluation of antimicrobial activity. Maximum antimicrobial activity of the biosurfactant (50μl) was observed in S. aureus (23 mm) and it was found that the biosurfactant activity was dependent on the concentration. So it could be used as a therapeutic agent in pharmaceutical application.
On Harder-Narasimhan reductions for Higgs principal bundles
Arijit Dey,R Parthasarathi
Mathematics , 2006,
Abstract: The existence and uniqueness of H-N reduction for the Higgs principal bundles over nonsingular projective variety is shown. We also extend the notion of H-N reduction for (\Gamma, G)-bundles and ramified G-bundles over a smooth curve.
Parabolic bundles on algebraic surfaces I- the Donaldson--Uhlenbeck compactification
V. Balaji,A. Dey,R. Parthasarathi
Mathematics , 2006,
Abstract: The aim of this paper is to construct the parabolic version of the Donaldson--Uhlenbeck compactification for the moduli space of parabolic stable bundles on an algenraic surface with parabolic structures along a divisor with normal crossing singularities. We prove the non--emptiness of the moduli space of parabolic stable bundles of rank 2 and also prove the existence of components with smooth points.
FORMULATION AND EVALUATION OF POSITIVELY CHARGED SELF-EMULSIFYING DRUG DELIVERY SYSTEM CONTAINING IBUPROFEN
Kulkarni. P. Parthasarathi,Dixit Mudit,R. Prudhvi,D. Lavanya
International Research Journal of Pharmacy , 2011,
Abstract: One of the most persistent problems faced by the formulation scientists has been to find methods of improving the oral bioavailability of poorly water-soluble drugs. This positively charged SEDDS gives several fold increase in the bioavailability than the negatively charged one. The present study was undertaken to formulate and evaluate a positively charged SEDDS containing Ibuprofen. Fifteen formulations were prepared and optimized for the concentration of surfactants, cationic lipid oleylamine, oil component and the formulation variables like speed and extent of stirring. The prepared formulations were characterized for emulsion type, drug content, zeta potential and in-vitro. In-vitro absorption profile of positively charged SEDDS was compared with that of negatively charged SEDDS and pure drug. The formulations were analysed by FT-IR and subjected for stability studies. The optimized formulation consists of 2% oleylamine, 30% surfactants (tween 80 and span 80 in 3:1 ratio) and 68% oil component. Zeta potentials of the formulations were found to be positive and this positively charged SEDDS showed better absorption profile than the negatively charged SEDDS. FT-IR results showed that the Ibuprofen was compatible with the excipients and the stability studies revealed that the formulation has enough shelf life.
Improvement of High Temperature Wear Resistance of AISI 316 ASS through NiCrBSiCFe Plasma Spray Coating  [PDF]
N. L . Parthasarathi, Muthukannan Duraiselvam
Journal of Minerals and Materials Characterization and Engineering (JMMCE) , 2010, DOI: 10.4236/jmmce.2010.97047
Abstract: In this work, the microstructural description, mechanical properties and dry sliding wear features of NiCrBSiCFe plasma sprayed on AISI 316 austenitic stainless steel (ASS) substrate were examined. Defect-free metallurgically bonded coatings were obtained. Wear tests were carried out at 2 m/s sliding velocity under the load of 20N for 2000 m sliding distance at various conditions like room temperature (35°C), 150°C, 250°C and 350°C using a EN-8 medium carbon steel pin as a counterface material. The properties namely coating density, micro hardness, coefficient of friction (CoF) and wear resistance of the coating were studied. NiCrBSiCFe plasma spray coated steel substrate showed superior wear resistance properties than the uncoated steel substrate in all the temperatures. The sliding wear resistance was improved up to 4.5 times than that of the uncoated material. In wear test carried out at 350oC, the coated substrates showed relatively lesser wear than in other temperatures due to the oxide formation, which acted as a protective layer. In addition, the shallow ploughing mode of wear reduced the severity of material removal at 350°C. Using scanning electron microscope (SEM) pictures, the wear properties of the coated steel substrate were evaluated in terms of plastic deformation, material transfer and abrasion.
INFLUENCE OF MOISTURE ON THE ACTIVITY OF PERIONYX EXCAVATUS (PERRIER) AND MICROBIAL-NUTRIENT DYNAMICS OF PRESSMUD VERMICOMPOST
K. Parthasarathi
Iranian Journal of Environmental Health Science & Engineering , 2007,
Abstract: Moisture play a crucial role in vermicomposting of pressmud (filter cake) (P). Five levels of moisture contents of pressmud (55-57%, 60-62%, 65-67%, 70-72% and 75-77%) at 31±2°C on earthworm activities (growth, reproduction and recovery rate of vermicompost) of Perionyx excavatus (Perrier)-an indigenous species and total microbial population, activity and nitrogen, phosphorous, potassium contents in the P vermicompost, over 60 days at an interval of 15, 30, 45 and 60 days have been studied. More and better worm biomass, cocoon production, hatchling number and rate of compost recovery were found in the 65-67% moisture. Besides this level, 70-72% and 60-62% moisture were adequate. On the other hand, 55-57% and 75-77% moisture did not have the desired effects on the growth and reproduction of earthworms and on vermicompost production. Enhanced microbial population and activity and nitrogen, phosphorous, potassium contents were found in fresh vermicomposts from 65-67% moisture than other moisture levels due to the ideal moisture of P for better multiplication of microbial population while passing through the worm gut with more activity, thereby enhancing the mineralization process resulting enhancement of nitrogen, phosphorous, potassium contents, whereas these decreased with decline in moisture content, immobilization and inactivation of microorganisms and/or increase in time (aging).
On how to create motivation in AIDS eye care
Roy Parthasarathi
Indian Journal of Ophthalmology , 2002,
Abstract:
Comments on: Central serous chorioretinopathy after dacryocystorhinostomy operation on the same side
Roy Parthasarathi
Indian Journal of Ophthalmology , 2010,
Abstract:
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