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Search Results: 1 - 10 of 4877 matches for " Paola Romina Amable "
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Identification of Appropriate Reference Genes for Human Mesenchymal Cells during Expansion and Differentiation
Paola Romina Amable, Marcus Vinicius Telles Teixeira, Rosana Bizon Vieira Carias, José Mauro Granjeiro, Radovan Borojevic
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0073792
Abstract: Background Quantitative real time polymerase chain reaction (qPCR) is an extremely powerful technique for monitoring gene expression. The quantity of the messenger ribonucleic acids (mRNA) of interest should be normalized using a reference gene, in order to avoid unreliable results originated by the obtained RNA quality and quantity, manipulation errors and inhibitory contaminants. A reference gene is any gene that is stably and consistently expressed under the conditions being studied. Completely false data can be generated if a reference gene is not chosen adequately. Results In the present study, we compared expression levels of five putative reference genes (HPRT1, ACTB, GAPDH, RPL13A and B2M) in primary cultures of four different human cells: mesenchymal stromal cells obtained from bone marrow, adipose tissue or umbilical cord Whartońs Jelly, and dermal fibroblasts, under different expansion and differentiation conditions. We observed that reference genes are not the same for different cells under the same culture conditions. Conclusion Most stable reference genes under our experimental conditions were: RPL13A for adipose tissue- and Whartońs Jelly-derived mesenchymal stromal cells, and HPRT1 for bone marrow-derived mesenchymal stromal cells and dermal fibroblasts. ACTB was the most unstable gene when evaluating adipose tissue- and Whartońs Jelly-derived mesenchymal stromal cells, whilst GAPDH and B2M were the most unstable genes for bone marrow-derived mesenchymal stromal cells and dermal fibroblasts, respectively.
DETERMINACIóN DE EDAD EN UNA POBLACIóN DE ZORRO COLORADO (Pseudalopex culpaeus) EN EL SUDESTE DE LA PROVINCIA DEL CHUBUT, ARGENTINA
ROMINA PAOLA LLANOS
Mastozoolog?-a neotropical , 2010,
Abstract:
Components Interoperability through Mediating Connector Patterns
Romina Spalazzese,Paola Inverardi
Electronic Proceedings in Theoretical Computer Science , 2010, DOI: 10.4204/eptcs.37.3
Abstract: A key objective for ubiquitous environments is to enable system interoperability between system's components that are highly heterogeneous. In particular, the challenge is to embed in the system architecture the necessary support to cope with behavioral diversity in order to allow components to coordinate and communicate. The continuously evolving environment further asks for an automated and on-the-fly approach. In this paper we present the design building blocks for the dynamic and on-the-fly interoperability between heterogeneous components. Specifically, we describe an Architectural Pattern called Mediating Connector, that is the key enabler for communication. In addition, we present a set of Basic Mediator Patterns, that describe the basic mismatches which can occur when components try to interact, and their corresponding solutions.
Ansiedad en situaciones de evaluación o examen, en estudiantes secundarios de la ciudad de Loja (Ecuador)
Amable Ayora
Revista Latinoamericana de Psicología , 1993,
Abstract: A research was carried out with high school students in Loja (Ecuador), in relation to test-anxiety. Navas (1991) Self-Assessment Inventory was applied to 45 students of both sexes, 17-19 years old. Results indicate a very high anxiety level in the students. More anxiety was found in women than in men. The implications for educational and psychological counseling are presented.
Políticas educativas, filosofía y planificación: la estrategia dominicana del Plan Decenal, 1990-2000
Amable Paulino
Ciencia y Sociedad , 2000,
Abstract: Este artículo provee una ilustración para el entendimiento de las filosofías, políticas y planificación educativa dominicana y por extensión para América Latina. El propósito del mismo es mirar a la estrategia del plan decenal de 1990 al 2000 desde una perspectiva de teorías políticas y enmarcarlo dentro de los campos de la educación comparativa, desarrollo nacional y economía global. El artículo está dirigido a dar una visión macro y fresca a la élite formuladora de políticas educativas. En este artículo, se niega la defensa de una posición dentro de la ecuación de intereses preestablecidos. También el artículo aspira a contribuir con una posición crítica positiva al dise o nacional educativo. En algunas momentos donde callo no implica una subscripción a la noción de callar para otorgar, sino, a dejar el espacio a otros para que superen estas informaciones necesarias pero no suficientes.
Atenuación natural y remediación inducida en suelos contaminados con hidrocarburos
Romaniuk,Romina; Brandt,Juan Felipe; Rios,Paola Ruth; Giuffré,Lidia;
Ciencia del suelo , 2007,
Abstract: the petroleum derived hydrocarbons represent the main energy source for the humanity and they are in turn, an important environmental polluting source. in this experience it was estimated and compared the rate of decomposition of two hydrocarbons (diesel and crankcase oil), by natural attenuation, and with the incorporation of a specific commercial product in a typic argiudoll. the effect of these practices was also evaluated on some soil variables. the treatments were: [control soil without pollution], [soil + gasoil], [soil + crankcase oil], [soil + gasoil + product], [soil + crankcase oil + product], [soil + product]. the soil sampling was carried out in the following series of time [0; 15; 30; 60 and 180 days]. the degradation rate of diesel and crankcase oil hydrocarbons by natural attenuation was slower and less effective than that obtained when adding the commercial product, the latter presented final lower values of total petroleum hydrocarbons. among the soil variables, the oxidable carbon and the biomass soil respiration registered higher mean values for the polluted soils. the commercial product incorporation produced a significant decrease in these values, an increase in the electric conductivity, but no influence on the ph and the soil extractable phosphorous.
Atenuación natural y remediación inducida en suelos contaminados con hidrocarburos Natural attenuation and induced remediation in hydrocarbon polluted soils
Romina Romaniuk,Juan Felipe Brandt,Paola Ruth Rios,Lidia Giuffré
Ciencia del Suelo , 2007,
Abstract: Los hidrocarburos derivados del petróleo constituyen la principal fuente de energía para la humanidad y son, a su vez, importantes contaminantes ambientales. En esta experiencia, se estimó y comparó la tasa de descomposición de dos tipos de hidrocarburos (gasoil y aceite de carter) por medio de las técnicas de atenuación natural y de la incorporación de un producto comercial específico en un suelo franco extraído del horizonte A de un Argiudol típico. Simultáneamente se evaluó el efecto de dichas prácticas tecnológicas sobre algunas variables edáficas. Los tratamientos fueron: [suelo sin contaminante testigo], [suelo + gasoil] [suelo + aceite de carter], [suelo + gasoil + producto], [suelo + aceite de carter + producto] y [suelo + producto]. La toma de muestra se realizó en la siguiente serie de tiempo [0;15; 30; 60; y 180 días]. La tasa de degradación de los hidrocarburos presentes en el gasoil y aceite de carter por la técnica de atenuación natural fue más lenta y menos efectiva que la obtenida al incorporar el producto comercial, alcanzando en este caso los valores finales más bajos de hidrocarburos totales de petróleo. Entre las variables edáficas, el carbono oxidable y la respiración de la biomasa del suelo mostraron los mayores valores medios en los tratamientos de suelo contaminado. La incorporación del producto comercial produjo una disminución significativa en dichos valores, un aumento en la conductividad eléctrica, pero no influyó sobre el pH y el fósforo extractable del suelo. The petroleum derived hydrocarbons represent the main energy source for the humanity and they are in turn, an important environmental polluting source. In this experience it was estimated and compared the rate of decomposition of two hydrocarbons (diesel and crankcase oil), by natural attenuation, and with the incorporation of a specific commercial product in a Typic Argiudoll. The effect of these practices was also evaluated on some soil variables. The treatments were: [control soil without pollution], [soil + gasoil], [soil + crankcase oil], [soil + gasoil + product], [soil + crankcase oil + product], [soil + product]. The soil sampling was carried out in the following series of time [0; 15; 30; 60 and 180 days]. The degradation rate of diesel and crankcase oil hydrocarbons by natural attenuation was slower and less effective than that obtained when adding the commercial product, the latter presented final lower values of Total Petroleum Hydrocarbons. Among the soil variables, the oxidable carbon and the biomass soil respiration registered higher mean values for the
Las clases sociales y la pobreza
Benach,Joan; Amable,Marcelo;
Gaceta Sanitaria , 2004, DOI: 10.1590/S0213-91112004000700005
Abstract: social classes and poverty are two key social determinants fundamental to understand how disease and health inequalities are produced. during the 90's in spain there has been a notable oscillation in the inequality and poverty levels, with an increase in the middle of the decade when new forms of social exclusion, high levels of unemployment and great difficulties in accessing the labour market, especially for those workers with less resources, emerged. today society is still characterized by a clear social stratification and the existence of social classes with a predominance of high levels of unemployment and precarious jobs, and where poverty is an endemic social problem much worse than the eu average. to diminish health inequalities and to improve the quality of life will depend very much on the reduction of the poverty levels and the improvement of equal opportunities and quality of employment. to increase understanding of how social class and poverty affect public health, there is a need to improve the quality of both information and research, and furthermore planners and political decision makers must take into account those determinants when undertaking disease prevention and health promotion.
Evaluación de la estructura para la producción e indicación de fitofármacos en adultos mayores con afecciones respiratorias. Colón, 1 ene.- 30 jun. de 2008 Evaluation of the structure for the production and indication of phytopharmaceutical products in elder people with respiratory diseases. Colon, January 1st-June 30th 2008
Adelaida García Amable
Revista M??dica Electr?3nica , 2012,
Abstract: El aumento de la esperanza de vida es uno de los mayores logros obtenidos en los avances en medicina y de la mejor calidad de vida de las personas. La población adulta mayor crece rápidamente en casi todos los países, por lo que urge tomar medidas encaminadas a proteger la salud y bienestar futuro de este grupo poblacional, prestando particular interés en la búsqueda de terapias menos agresivas y sin efectos secundarios. Esta investigación evaluó la disponibilidad de recursos humanos y materiales para la atención al tratamiento con fitofármacos de los adultos mayores con en respiratorias, en el área de salud del Policlínico Dr. Francisco Figueroa Véliz, del municipio Colón, provincia de Matanzas. El dise o metodológico utilizado responde a los principios, leyes y categorías del materialismo dialéctico y su método científico. Para la recolección de información se utilizó un instrumento confeccionado por el equipo de trabajo para estos fines y validado en su funcionamiento. Se encontró que todas las unidades organizativas del área de salud presentan problemas con los recursos disponibles que conforman su estructura. The increase of the life-expectancy is one of the highest achievements obtained as a result of the medicine advances and the better people’s life quality. Elder people population grows fast almost in all the countries, so it is necessary to introduce measures to protect the future wellbeing and health of this group of population, giving particular attention to less aggressive therapies, without secondary effects. This research evaluated the availability of the human resources and materials to treat elder people with respiratory diseases with phytopharmaceutical products in the health area of the Policlinic Dr. Francisco Figueroa Veliz, of the municipality of Colon, province of Matanzas. The methodological design used is in correspondence with the principles, laws and categories of the dialectic materialism and its scientific method. To collect data we used an instrument elaborated by the working team with this aim and validated in its functioning. We found that all the organizational units of the health area have problems with the available resources forming their structures.
Ni os con déficit de atención e hiperquinesis (TDA/H)
Amable Ayora Fernández
Revista Latinoamericana de Psicología , 2004,
Abstract:
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