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Search Results: 1 - 10 of 1422 matches for " Padilla-Docal "
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Neuroinmunological response in HIV-associated Guillain-Barre syndrome: A case report  [PDF]
Ivonne M. Iglesias-González, Alberto J. Dorta-Contreras, Bárbara Padilla-Docal, Maylin Victoria-Garcia, Ricardo Junco-Calzadilla
World Journal of Neuroscience (WJNS) , 2012, DOI: 10.4236/wjns.2012.21005
Abstract: Introduction: Human immunodeficiency virus infec- tion is associated with several different types of peripheral neuropathy: distal predominantly sensory axonal polyneuropathy, like Guillain Barre syndrome. Case presentation: A 55-year-old Caucasian woman with Human immunodeficiency virus infection, diag- nosed with Guillain Barre syndrome was studied. Serum and CSF immunoglobulin G and Albumin levels were quantified by using an immunodiffusion technique. She had preceding viral symptoms. The clinical diagnosis of the illness in this patient was pa-resthesias or sensory loss, tendon reflexes. It was also observed cranial nerves abnormalities, acute mo- tor and sensory axonal neuropathy and ophthalmoplegia. The mean CD4 count was 367/mm3, CSF white blood cell 25 wbc/mm3. The serum sodium concentration was significantly low (133 mmol/L). She had a respiratory compromise as a result of their neuropa- thy and developed congestive heart failure and hy- potension and died of a cardiac arrest. The neuro-immunological response described by our patient was a blood/CSF barrier dysfunction without IgG intra-thecal synthesis. Conclusion: There is no doubt that this study is of great importance because will help clinicians increase their knowledge of the immune response in patients with this autoimmune disorder on the basis of this case report, in which, for first time, could be seen the neuroimmunological response through the reibergram in a patient with HIV- asso-ciated Guillain-Barre syndrome.
Mannan-binding lectin in cerebrospinal fluid: a leptomeningeal protein
Hansotto Reiber, Barbara Padilla-Docal, Jens Jensenius, Alberto Dorta-Contreras
Fluids and Barriers of the CNS , 2012, DOI: 10.1186/2045-8118-9-17
Abstract: MBL was assayed in samples of CSF and serum with an ELISA, coated with anti MBL antibodies. Routine parameters such as albumin-, immunoglobulin- CSF/serum quotients, oligoclonal IgG and cell count were used to characterize the patient groups. Groups comprised firstly, control patients without organic brain disease with normal CSF and normal barrier function and secondly, patients without inflammatory diseases but with increased QAlb, i.e. with a blood CSF barrier dysfunction.MBL concentration in CSF was at least five-fold higher than expected for a molecular-size-dependent passage from blood. Secondly, in a QIgM/QAlb quotient diagram (Reibergram) 9/13 cases showed an intrathecal fraction in some cases over 80% of total CSF MBL concentration 3) The smaller inter-individual variation of MBL concentrations in CSF of the control group (CV?=?66%) compared to the MBL concentrations in serum (CV?=?146%) indicate an independent source of MBL in CSF. 4) The absolute MBL concentration in CSF increases with increasing QAlb. Among brain-derived proteins in CSF only the leptomeningeal proteins showed a (linear) increase with decreasing CSF flow rate, neuronal and glial proteins are invariant to changes of QAlb.MBL in CSF is predominantly brain-derived and all results pointed to the leptomeningeal cells as the source of the protein. The evaluation of this protein requires the interpretation of its absolute concentrations in CSF as a function of the albumin quotient, QAlb. This recognition of MBL in brain cells opens a new field of discussion about the function of the innate immune response in CNS in cases of acute and chronic neurological diseases.Increased protein concentrations in the cerebrospinal fluid (CSF) of patients with neurological diseases, frequently ascribed to a blood-CSF barrier dysfunction, are due to pathologically-reduced CSF flow rates [1]. This view is based on the molecular diffusion/CSF flow theory [1] which shows that the concentration of a blood-derived pr
Using reibergrams to evaluate the intrathecal synthesis of C3c and C4 in children affected with Neisseria meningitidis meningoencephalitis
Dorta-Contreras,Alberto J; Padilla-Docal,Bárbara; Coifiu-Fanego,Raisa B;
Biotecnolog?-a Aplicada , 2011,
Abstract: meningococcal meningoencephalitis was a serious pediatric health problem in cuba before the successful introduction of a cuban vaccine against neisseria meningitidis. the present paper assesses the role of the complement system in this disease in unvaccinated sick children, using a novel methodology developed by the authors. seven children were used, of an average of 5.8 years of age with n. meningitidis meningoencephalitis diagnosed by traditional microbiological methods. serum and cerebrospinal fluid samples were obtained at the same time point, and used to quantify major immunoglobulins, igg subclasses, c3c, c4 and albumin by radial immunodiffusion with commercially available plates. no c3c was found in one of the two deceased patients. measurable intrathecal synthesis of c3c was observed in the remaining patients, however, intrathecal synthesis of c4 was found in all patients, as demonstrated with the corresponding reibergrams. the measurement of the intrathecal synthesis of these components of the complement system is useful for discriminating immunodeficiencies and to better understand the behavior of the disease.
CSF/serum quotient graphs for the evaluation of intrathecal C4 synthesis
Barbara Padilla-Docal, Alberto J Dorta-Contreras, Raisa Bu-Coifiu-Fanego, Alexis Rey
Fluids and Barriers of the CNS , 2009, DOI: 10.1186/1743-8454-6-8
Abstract: The constants in the previously-defined Reibergram for immunoglobulin IgA were used to calculate the CSF/serum quotient for C4. CSF and serum were analyzed for C4, IgA and albumin from a total of 12 patients with meningoencephalitis caused by encapsulated microorganisms and 10 subjects without infections or inflammatory neurological disease, some of which had dysfunction of the blood-CSF barrier,The formula and C4 Reibergram with the constants previously found for IgA, determined the intrathecal C4 synthesis in CSF. The intrathecal C4 fraction in CSF (C4 loc in mg/l) was compared to the C4-Index (fraction of CSF: serum for C 4/fraction of CSF: serum for albumin). There was a significant correlation between the two formulae. The CSF/Serum quotient graph was superior for detecting intrathecal synthesis of C4 under variable conditions of blood-CSF barrier permeability.The C4 Reibergram can be used to quantify the intrathecal synthesis of this component of the complement system in different infectious diseases of the central nervous system and is especially useful for patients with blood-brain barrier dysfunction.Cerebrospinal fluid (CSF) analysis has great potential for the diagnosis of neurological diseases. Reibergrams or CSF/serum quotient graphs [1,2] are diagrams that analyze in an integrated way both the function of the blood-CSF barrier and intrathecal protein synthesis, to aid in the diagnosis of central nervous system (CNS) diseases associated with specific patterns of immunoglobulin response. This diagram was first defined for the major classes of immunoglobulins [2,3] empirically based on the results of thousands of profiles and was subsequently confirmed by application of Fick's law of diffusion in the theory of molecular diffusion/flow rate [4]. Fick's law states that the diffusion of a protein through a barrier depends on the diffusion coefficient of the molecule and on the concentration gradient between the compartments on either side of the barrier. The
Respuesta neuroinmunológica en ni os con meningoencefalitis por Neisseria meningitidis.
Bárbara Padilla-Docal,Rosa Ferro-Blanco,Alberto Juan Dorta-Contreras,Raisa Bu Coifiu-Fanego
Vaccimonitor , 2011,
Abstract: La enfermedad meningocócica es una entidad clínica que constituye aún un problema de salud mundial. Esta afecta, sobre todo, a la población infantil ocasionando un cuadro clínico grave, de evolución severa, así como un elevado número de muertes y secuelas. Su agente etiológico, Neisseria meningitidis, habita de forma natural enlas membranas mucosas de la nasofaringe humana. Con el objetivo de determinar las características de la respuesta neuroinmunológica en ni os con meningoencefalitis por N. meningitidis, se estudiaron 20 ni os diagnosticados con esta afección clínica, entre los a os 1988- 2009. A estos se les realizó una punción lumbar en el momento del ingreso y se cuantificó la IgA, IgM e IgG, la albúmina y el C3c en el suero y en el líquido cefalorraquídeo.Se confeccionó un reibergrama de acuerdo con los datos obtenidos en la cuantificación. La disfunción de la barrera hematoencefálica se presentó en seis pacientes y hubo síntesis intratecal de las inmunoglobulinas mayores y C3c en 65% y 95%, respectivamente. Se comprobó la ocurrencia de síntesis intratecal de al menos dos inmunoglobulinas mayores asociadas con el componente C3c del complemento, participando estos en los mecanismos involucrados en la respuesta inmune de esta enfermedad.
Síntesis intratecal de C3c e inmunoglobulinas en ni os con meningoencefalitis bacteriana.
Bárbara Padilla-Docal,Alberto Juan Dorta-Contreras,Raisa Bu Coifiu-Fanego,Elena Noris-García
Vaccimonitor , 2008,
Abstract: Las meningoencefalitis bacterianas constituyen una fuente importante de morbilidad, mortalidad y discapacidad en diferentes regiones del mundo. El objetivo del presente trabajo es conocer si el sistema de complemento puede estar involucrado en la lisis de las bacterias productoras de meningoencefalitis a través de la liberación de C3c al líquido cefalorraquídeo. Se estudiaron siete pacientes con edad promedio de 3 a os, que ingresaron en el Hospital Pediátrico de San Miguel del Padrón, a los que se les realizó una punción lumbar diagnóstica y se les aislaron los gérmenes siguientes: Neisseria meningitidis, Streptococcus pneumoniae y Haemophilus influenzae. La cuantificación de los niveles de C3c, albúmina e inmunoglobulinas mayores en suero y líquido cefalorraquídeo se realizó en placas de inmunodifusión radial. Los resultados obtenidos fueron recogidos en un reibergrama. El total de los pacientes estudiados mostraron síntesis intratecal del componente C3c del sistema de complemento. Este hecho evidenció la activación de este sistema en alguna de sus vías y que una vez cumplidas sus funciones biológicas, ha sufrido un proceso de degradación y liberación al LCR en forma de C3c.
Reibergrama para la evaluación de la síntesis intratecal de C3c
Dorta-Contreras Alberto Juan,Noris-García Elena,Padilla-Docal Bárbara,Rodríguez-Rey Alexis
Arquivos de Neuro-Psiquiatria , 2006,
Abstract: INTRODUCCIóN: El diagrama de las razones de Reiber o reibergrama cobra cada día mayores usos para la caracterización de la síntesis intratecal de proteínas. El reibergrama fue definido para las clases mayores de inmunoglobulinas pero luego ha sido utilizado para evaluar otras proteínas basado en la teoría de la difusión molecular/velocidad de flujo del líquido cefalorraquídeo (LCR). MéTODO: El C3c, producto de la degradación del factor del complemento C3 y con una masa molecular de 145 KDa, se acerca a las características moleculares de la IgG para las leyes de la difusión de Fick. Se asume las constantes de la IgG en la fórmula de Reiber para evaluar la síntesis intratecal de C3c así como su correspondiente reibergrama. Se estudiaron 27 pacientes y 27 controles a los que se les dosificó albúmina y C3c en suero y LCR por inmunodifusión radial. RESULTADOS: Con el reibergrama propuesto para el C3c se evaluaron estos pacientes. Se comprueba la validez de este reibergrama para distintas condiciones de barrera con o sin síntesis intratecal de C3c. CONCLUSION: El reibergrama y su fórmula correspondiente propuesto para la C3c puede ser usado para la evaluación de la síntesis intratecal de C3c.
Intrathecal synthesis of IgE in children with eosinophilic meningoencephalitis caused by Angiostrongylus cantonensis
Barbara Padilla-Docal, Alberto J Dorta-Contreras, Raisa Bu-Coifiu-Fanego, Hermes Hernández, Jesús Barroso, Consuelo Sanchez-Martinez
Fluids and Barriers of the CNS , 2008, DOI: 10.1186/1743-8454-5-18
Abstract: Thirteen patients, mean age 4.5 years were studied; a diagnostic lumbar puncture was performed and serum samples taken. Immunoglobulin E (IgE) in serum and in cerebrospinal fluid (CSF) was quantified by nephelometry. Control patients had other infections or other neurological diseases.The mean cell count in the CSF was 500 × 10-6 cells/L and of these 23% were eosinophils. In blood the eosinophils were 13%. The chief symptoms of the patients were migraine, vomiting and fever and 50% presented some meningeal signs. IgE intrathecal synthesis analyzed by the corresponding quotient diagram (Reibergram) was observed in all patients. No intrathecal IgE synthesis was seen in control patients.Intrathecal synthesis of IgE demonstrates the participation of this immunoglobulin in the destruction of the third stage larvae of the parasite in the CSF. The test should be considered in our environment as a tool to aid diagnosis.Eosinophilic meningoencephalitis is a disease caused by the helminth Angiostrongylus cantonensis. The definitive hosts of this parasite are rats, Rattus rattus and Rattus norvegicus [1-3]. Many species of mollusc constitute intermediary hosts [4] and are responsible for the transmission of this zoonosis. Eosinophilic meningoencephalitis described for the first time in Southeast Asia and later in Asia, Africa and the Caribbean [5-8], is considered an important and sometimes fatal human disease. In Cuba this disease is primarily observed in children with a mild course, because the number of larvae accidentally ingested is small. Since 1981, when in the Paediatric Hospital of San Miguel del Padron, the first case was observed in the Americas, an average of 3 cases per year have been reported.IgE plays an important role in anaphylactic type 1-hypersensitivity mechanisms, with high values in patients with parasitic infectious diseases accompanied by eosinophilia [5].In an earlier study [9] on four patients diagnosed with eosinophilic meningoencephalitis caused by
Neuroimmunological findings of Angiostrongylus cantonensis meningitis in ecuadorian patients
Dorta-Contreras, Alberto J.;Padilla-Docal, Barbara;Moreira, Juan M.;Robles, Luiggi Martini;Aroca, Jenny Muzzio;Alarcón, Fernando;Bu-Coifiu- Fanego, Raisa;
Arquivos de Neuro-Psiquiatria , 2011, DOI: 10.1590/S0004-282X2011000400011
Abstract: meningitis caused by angiostrongylus cantonensis has recently been reported in patients resulting from the first outbreaks in subtropical regions of ecuador. method: eight young adult patients from the two outbreaks were studied. iga, igm, igg and albumin in cerebrospinal fluid and serum were quantified and plotted in cerebrospinal fluid/serum quotient diagrams (reibergrams). the anamnesis on the patients included asking about any consumption of raw snails, symptoms and harm caused. results: mean eosinophilia of 7.5% and 26% in serum and cerebrospinal fluid respectively was observed, as well as a moderate increase in total proteins. the most frequent pattern of intrathecal synthesis was observed in three classes of immunoglobulins. intrathecal synthesis of igm was observed in all cases two weeks after the first symptoms appeared. conclusion: the intrathecal synthesis patterns of eosinophilic meningitis due to angiostrongylus cantonensis, facilitated by cerebrospinal fluid analysis, were similar to those of previous cases from abroad.
Clinical behavior of Streptococcus pneumoniae meningoencephalitis
Bu-Coifiu Fanego, Raisa;Dorta-Contreras, Alberto J.;Padilla-Docal, Bárbara;O' Farril-Sanchez, Martha;Lopez-Hernandez, Isabel;
Arquivos de Neuro-Psiquiatria , 2009, DOI: 10.1590/S0004-282X2009000600012
Abstract: objective: there was an increased number of cases of meningoencephalitis caused by streptococcus pneumoniae, after the successful vaccination campaigns against neisseria meningitidis and haemophilus influenzae. this paper aims at describing the clinical characteristics, the laboratory findings, the complications, and the therapeutic management of these patients, who have been suffering from this disease since 1993 to 2006. method: twelve children with streptococcus pneumoniae meningoencephalitis admitted to the pediatric hospital of san miguel del padron, city of havana in this period were assessed. results: children under one year are the most frequently affected. septic shock and brain edema were the most severe complications. three patients died, implying that this disease has a serious course. early treatment of brain edema is very important to reduce mortality. the elective drugs for treatment of these cases of streptococcus pneumoniae meningoencephalitis were vancomycin combined with cephalosporin, cefotaxime or ceftriaxone type. conclusion: patients with streptococcus pneumoniae meningoencephalitis show clinical characteristics, complications, and sequels that are different to other bacterial meningoencephalitis, meaning that they could be helpful for physicians considering the differential diagnosis of meningoencephalitis.
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