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Search Results: 1 - 10 of 336915 matches for " Paciullo Domingos Sávio Campos "
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CARACTERíSTICAS ANAT?MICAS RELACIONADAS AO VALOR NUTRITIVO DE GRAMíNEAS FORRAGEIRAS
Paciullo, Domingos Sávio Campos;
Ciência Rural , 2002, DOI: 10.1590/S0103-84782002000200029
Abstract: the tissue proportion and cell wall thickness are important anatomical traits influencing nutritive value of grasses. theses characteristics present high correlation with fiber, lignin and crude protein (cp) contents and in vitro dry matter digestibility (ivdmd) coefficients. the tissues lowest digested correlate negatively with cp and ivdmd and positively with fiber and lignin contents, while the tissues rapidly digested show positive correlation with cp and ivdmd and negative with fiber and lignin contents. the digestion of some tissues is limited not only by negative effect of lignin on the cell wall digestion, but too by compact organization of cells of some tissues and by high cell wall thickness. the relationships between plant anatomy and nutritive value highlight the possibility of utilization of anatomical traits in evaluation of forage grass nutritive value.
CARACTERíSTICAS ANAT MICAS RELACIONADAS AO VALOR NUTRITIVO DE GRAMíNEAS FORRAGEIRAS
Paciullo Domingos Sávio Campos
Ciência Rural , 2002,
Abstract: Entre as características anat micas relacionadas ao valor nutritivo de gramíneas, destacam-se a propor o de tecidos e a espessura da parede celular. Tais características apresentam altas correla es com os teores de fibra, de lignina e de proteína bruta (PB) e com os coeficientes de digestibilidade in vitro da matéria seca (DIVMS). Os tecidos de baixa digest o correlacionam-se negativamente com a PB e a DIVMS e positivamente com os teores de fibra e de lignina, enquanto aqueles rapidamente digeridos mostram correla es positivas com a PB e com a DIVMS e negativas com os teores de fibra e lignina. A digest o de alguns tecidos é limitada, n o somente pelo efeito negativo da lignina sobre a digest o da parede celular, mas também pelo compacto arranjo das células de alguns tecidos e pela elevada espessura da parede celular. As rela es entre a anatomia da planta e o valor nutritivo real am a possibilidade da utiliza o de características anat micas na avalia o do valor nutritivo de gramíneas forrageiras.
Use of Ultrasound Bath in the Extraction and Quantification of Ester-Linked Phenolic Acids in Tropical Forages  [PDF]
Mellina Damasceno Rachid Santos, Aline de Paula Vitor, Jailton da Costa Carneiro, Domingos Sávio Campos Paciullo, Renato Camargo Matos, Maria Auxiliadora Costa Matos
American Journal of Analytical Chemistry (AJAC) , 2011, DOI: 10.4236/ajac.2011.23042
Abstract: A method was developed for the analysis of ester-linked phenolic acids in forage samples using extraction by an ultrasound-assisted treatment and quantification by HPLC with a UV-VIS detector. A reversed-phase C18 column was used for developing the method and the optimal condition was established with isocratic elution using acetonitrile/methanol/H3PO4 pH 2.08 (13:12.5:74.5) as the mobile phase. To reduce the time of sample processing, the extraction of ester-linked phenolic acids was studied using ultrasound bath and the results were then compared with those from an extraction usual using alkaline hydrolysis (20°C for 24 h). The method was valued through external and internal calibration. Internal calibration using o-coumaric acid as internal standard and m-coumaric acid as surrogate internal standard showed better results. The detection limits were of 0.09 and 0.04 mg●L-1 for p-coumaric and ferulic acids, respectively. The proposed method showed a good linear dynamic range (3.00 - 30.00 mg●L-1) for the analytes. The usefulness of the methodology was demonstrated by addition-recovery experiments using forage samples and values were in the 83 to 99% range. The extraction of ester-linked phenolic acids by 120 minutes of ultrasound bath was faster and more reproducible than alkaline hydrolysis (20°C for 24 h).
Composi??o química e digestibilidade in vitro de laminas foliares e colmos de gramíneas forrageiras, em fun??o do nível de inser??o no perfilho, da idade e da esta??o de crescimento
Paciullo, Domingos Sávio Campos;Gomide, José Alberto;Queiroz, Domingos Sávio;Silva, Eldo Ant?nio Monteiro da;
Revista Brasileira de Zootecnia , 2001, DOI: 10.1590/S1516-35982001000400009
Abstract: chemical composition and the in vitro dry matter digestibility were evaluated in blades and stems at two stages of development and two insertion levels on tillers of signalgrass (brachiaria decumbens), molassesgrass (melinis minutiflora) and tifton 85 bermudagrass (cynodon sp), in two seasons of the year (summer and autumn). lower and upper leaf blades were sampled from the tillers, on the day of ligule exposure and 20 days thereafter, and stems segments located below of the upper leaf blade. the crude protein contents of leaf blades showed little differences among the species, but decreased with development from 18.8 to 14.6% and level of insertion from 18.9 to 14.5% from lower to upper position. leaf blades of higher insertion level had a greater neutral detergent fiber and lignin concentrations. the ivdmd decreased as leaf aged and exhibited greater values when situated at lower insertion level and sampled in the autumn. the ivdmd of old leaf blades were higher to tifton 85 bermudagrass and lower to signalgrass. the average values of ivdmd of stems varied from 56.7 to 62.7%, according to stage of development and season of growth. the values were 56.9, 59.6 and 60.5% for the signalgrass, bermudagrass and molassesgrass, respectively.
Correla??es entre componentes anat?micos, químicos e digestibilidade in vitro da matéria seca de gramíneas forrageiras
Paciullo, Domingos Sávio Campos;Gomide, José Alberto;Queiroz, Domingos Sávio;Silva, Eldo Ant?nio Monteiro da;
Revista Brasileira de Zootecnia , 2001, DOI: 10.1590/S1516-35982001000400008
Abstract: leaf blades and stem segments at two stages of development (day of leaf ligule exposure and 20 days thereafter) and two insertion levels on tillers (lower and upper) of signalgrass (brachiaria decumbens), molassesgrass (melinis minutiflora) and tifton 85 bermudagrass (cynodon sp), were sampled in two seasons of the year (summer and autumn). the anatomical and chemical traits, and in vitro dry matter digestibility of blades and stems segments were evaluated. the correlations among these variables were established to associated values observed in leaf blade and stem, and to each fraction. the cell wall thickness was the anatomical trait to exhibit the highest correlation with all the chemical components, being positive with neutral detergent fiber, acid detergent fiber and lignin contents and negative with crude protein content. the proportion of mesophyll correlated positively with crude protein and negatively with acid detergent fiber content, while the proportion of sclerenchyma showed positive correlation with neutral detergent fiber content. the chemical components showed strong correlation among themselves and with the ivdmd. from the anatomical traits, the cell wall thickness was the only one to exhibit a significant correlation with the ivdmd, independent of fraction. the ivdmd correlated positively with leaf blade mesophyll and negatively with stem xylem.
Composi o química e digestibilidade in vitro de laminas foliares e colmos de gramíneas forrageiras, em fun o do nível de inser o no perfilho, da idade e da esta o de crescimento
Paciullo Domingos Sávio Campos,Gomide José Alberto,Queiroz Domingos Sávio,Silva Eldo Ant?nio Monteiro da
Revista Brasileira de Zootecnia , 2001,
Abstract: Laminas foliares e segmentos de colmo das gramíneas forrageiras capim-braquiária (Brachiaria decumbens), capim-gordura (Melinis minutiflora) e capim-bermuda Tifton 85 (Cynodon sp) foram analisados quanto aos teores de proteína bruta (PB), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA), celulose, hemicelulose e lignina e aos coeficientes de digestibilidade in vitro da matéria seca (DIVMS), em duas esta es de crescimento: ver o e outono. As laminas, amostradas no momento da exposi o da lígula e 20 dias após, situavam-se nas posi es inferior e superior do perfilho. Os segmentos de colmo avaliados foram os situados imediatamente abaixo da lamina da posi o superior. O colmo de capim-bermuda Tifton 85 apresentou 8,5 unidades percentuais a menos de FDA que os das outras duas gramíneas, que n o diferiram entre si. Os teores médios de PB de laminas foliares exibiram pequena varia o entre as espécies e diminuíram de 18,8 para 14,6%, após 20 dias de expans o, e de 18,9 para 14,5%, entre as laminas das posi es inferior e superior do perfilho. Laminas da posi o superior apresentaram mais elevados teores de FDN e lignina, independente da espécie, da esta o e da idade. Os coeficientes de DIVMS reduziram a partir do momento da expans o da lamina foliar e foram maiores em laminas situadas no nível inferior e colhidas no outono. Entre as espécies, a DIVMS n o variou em laminas recém-expandidas, mas foi mais elevada em capim-bermuda Tifton 85 e mais baixa em capim-braquiária em laminas com 20 dias após completa expans o. A DIVMS do colmo foi mais baixa em capim-braquiária (56,9%) e mais elevada em capim-bermuda Tifton 85 (59,6%) e capim-gordura (60,5%), que n o diferiram entre si.
Correla es entre componentes anat micos, químicos e digestibilidade in vitro da matéria seca de gramíneas forrageiras
Paciullo Domingos Sávio Campos,Gomide José Alberto,Queiroz Domingos Sávio,Silva Eldo Ant?nio Monteiro da
Revista Brasileira de Zootecnia , 2001,
Abstract: Laminas foliares e segmentos de colmo das gramíneas forrageiras capim-braquiária (Brachiaria decumbens), capim-gordura (Melinis minutiflora) e capim-bermuda Tifton 85 (Cynodon sp) foram amostradas em dois níveis de inser o no perfilho (inferior e superior), em duas idades (momento da exposi o da lígula da folha e 20 dias após) e em duas esta es de crescimento (ver o e outono). Amostras dos segmentos de colmo e laminas foliares foram submetidas à determina o de sua composi o química, composi o anat mica e digestibilidade in vitro. Após obten o dos dados, foram estabelecidas as correla es entre os componentes químicos e anat micos e entre estes e a DIVMS, na lamina, no colmo e no agrupamento dos dados das duas fra es. A espessura da parede celular foi a característica anat mica a se correlacionar mais fortemente com todos os componentes químicos, independente da fra o considerada. Suas correla es foram positivas com os teores de fibra em detergente neutro, fibra em detergente ácido e lignina e negativa com os de proteína bruta. A propor o de mesofilo se correlacionou positivamente com os teores de proteína bruta e negativamente com os de fibra em detergente ácido, enquanto a propor o de esclerênquima apresentou correla o positiva com os teores de fibra em detergente neutro. Os componentes químicos se correlacionaram fortemente entre si e com a DIVMS. Entre as características anat micas, somente a espessura da parede celular mostrou correla o significativa com a DIVMS, independente da fra o. O mesofilo se correlacionou positivamente e o xilema negativamente com a DIVMS, respectivamente, na lamina e no colmo.
Aduba??o nitrogenada do capim-elefante cv. Mott: 2. valor nutritivo ao atingir 80 e 120 cm de altura
Ribeiro, Karina Guimar?es;Gomide, José Alberto;Paciullo, Domingos Sávio Campos;
Revista Brasileira de Zootecnia , 1999, DOI: 10.1590/S1516-35981999000600004
Abstract: this trial was carried out, from october 1993 until june 1995, to evaluate the chemical composition and dry matter digestibility of dwarf elephantgrass at plant heights of 80 and 120 cm under five nitrogen rates. the experimental design was completely randomized with a split split plot arrangement, with four replicates, where the clipping factors, and nitrogen doses (0, 25, 50, 100kg/ha?clipping, in the first year, and 0, 75 and 100 kg ha?clipping, in the second year, and plant heights (80 and 120 cm) and its effects on the grass performance to yield were studied. the crude protein content in the leaf blades ranged, in the first year, from 8.8 to 13.2, in plants that reached 80 cm, and from 9.7 to 11,3% in plants with 120 cm, and in average, from 9.6 to 12.6%, in the second year, in response to the doses of nitrogen. the neutral detergent fiber, that was of 71.7% in the first year, varied from 67.4 to 71.1%, in plants with 80 and 120 cm, respectively, in the second year. the observed coefficients of ivdmd were 66.3 and 64.9% for plant heights of 80 and 120 cm, in the first year, and 69.0 and 61.5, in the second year, in the leaf blades of plants with 80 and 120 cm, respectively. the p, k and ca contents in the leaf blade inversely varied with the nitrogen doses, while the mg content did not present variation.
Características anat?micas da lamina foliar e do colmo de gramíneas forrageiras tropicais, em fun??o do nível de inser??o no perfilho, da idade e da esta??o de crescimento
Paciullo, Domingos Sávio Campos;Gomide, José Alberto;Silva, Eldo Ant?nio Monteiro da;Queiroz, Domingos Sávio;Gomide, Carlos Augusto Miranda;
Revista Brasileira de Zootecnia , 2002, DOI: 10.1590/S1516-35982002000400012
Abstract: an experiment was carried out to evaluate the effect of leaf age and insertion level and season of growth on the tissue proportion and the cell wall thickness of leaf blade and stem segment of signalgrass (brachiaria decumbens), molassesgrass (melinis minutiflora) and tifton 85 bermudagrass (cynodon sp). leaf blades from bottom and top layers were sampled on the day of ligule exposure and 20 days later. the relative proportion of epidermis, xylem, phloem, parenchyma bundle sheath, sclerenchyma, stem parenchyma and leaf blade mesophyll were estimated. the thickness of cell wall of metaxylem vessels and sclerenchyma were evaluated. leaf tissue proportion changed neither with age nor season of growth; thickness of leaf sclerenchyma cell wall was the only trait to change with age. leaf from the top layer showed higher proportion of sclerenchyma, parenchyma bundle sheath and xylem as well as thicker cell wall in sclerenchyma and metaxylem; while leaves from the bottom layer had higher proportion of mesophyll and thinner sclerenchyma and metaxylem cell walls. while the parenchyma proportion of the stem decreased, the relative area of the sclerenchyma and the cell wall thickness increased as the plant aged. these traits showed greater values in summer samples.
Degrada??o in vitro de tecidos da lamina foliar e do colmo de gramíneas forrageiras tropicais, em fun??o do estádio de desenvolvimento
Paciullo, Domingos Sávio Campos;Gomide, José Alberto;Silva, Eldo Ant?nio Monteiro da;Queiroz, Domingos Sávio;Gomide, Carlos Augusto Miranda;
Revista Brasileira de Zootecnia , 2002, DOI: 10.1590/S1516-35982002000400013
Abstract: a trial was carried out to evaluate the change in digestion of tropical grasses leaf and stem tissues with age. the grasses were signalgrass (brachiaria decumbens), molassesgrass (melinis minutiflora) and tifton 85 bermudagrass (cynodon sp). the 7th leaf of signalgrass and molassesgrass and the 11th leaf of bermudagrass were sampled by the time of their complete expansion (ligule exposure) and 20 days later. segment of stem just below the sampled leaf was also sampled. transversal sections of leaf and stem were examined at the microscope before and after rumen in vitro incubation. the degree of digestion of different leaf and stem tissues, as well as the cell wall thickness of stem sclerenchyma were estimated. leaf blade and young stems showed larger digested areas. stem tissues with thick and lignified cell wall, the parenchyma bundle sheath, the sclerenchyma, the xylem and the epidermis were not digested. tissues with thin non-lignified cell wall, the mesophyll, the phloem and the parenchyma were completely digested. molassesgrass and signalgrass leaves sampled 20 days after expansion had lower mesophyll digestion in relation to their recently expanded leaves. similarly, molassesgrass stem parenchyma digestion decreased as plant aged. regardless of age, leaf epidermis was only partially digested. stem sclerenchyma cell wall showed percentage reduction in thickness from 7 to 37%, after in vitro incubation. the rate of reduction in thickness varied from 0.007 to 0.018 μm/h.
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