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Search Results: 1 - 10 of 463068 matches for " PATRICIO ACU?A "
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Efecto de tratamiento pregerminativo sobre la germinación de semillas de Eucryphia glutinosa (Poepp. et Endl.) Baillon
BREVIS ACUA,PATRICIO;
Bosque (Valdivia) , 2003, DOI: 10.4067/S0717-92002003000200009
Abstract: the primary objective of this study was to establish the effects of chilling pretreatment and duration on both total germination and germination rate for seeds of eucryphia glutinosa (poepp. et endl.) baillon. seeds were kept at 3-4°c on dry and wet sand (dry chilling and stratification, respectively) for 30, 60 and 90 days. a control (no chilling) was evaluated at 30 day intervals simultaneously with pretreated seeds. germination was carried out in a chamber at 23°c. average overall germination percentage was 77.0 ±6.8 %. germination capacity of seeds was affected neither by pretreatment nor duration. treatments had an effect on germination rate, with seeds stratificated for 60 and 90 days showing significantly higher rates of germination. seeds stored for six months since harvest at room temperature did not show changes in germination capacity and rate of germination.
Efecto de tratamiento pregerminativo sobre la germinación de semillas de Eucryphia glutinosa (Poepp. et Endl.) Baillon Effect of pretreatment on germination of seeds of Eucryphia glutinosa (Poepp. et Endl.) Baillon
PATRICIO BREVIS ACUA
Bosque (Valdivia) , 2003,
Abstract: El propósito de este trabajo fue determinar los efectos de tratamiento pregerminativo con frío y tiempo de aplicación sobre la capacidad germinativa y la velocidad de germinación de semillas de Eucryphia glutinosa (Poepp. et Endl.) Baillon. Las semillas se mantuvieron a 3-4°C en arena seca y húmeda (enfriamiento seco y estratificación, respectivamente) durante 30, 60 y 90 días. Un control fue evaluado a intervalos de 30 días junto con las semillas tratadas. La germinación se llevó a cabo en una cámara a 23°C. En promedio el porcentaje de germinación fue 77,0 ± 6,8%. La capacidad germinativa de las semillas no se vio afectada por los tratamientos pregerminativos ni por los tiempos de aplicación. Los tratamientos tuvieron efecto sobre la velocidad de germinación, siendo estratificación por 60 y 90 días los que mostraron las mayores tasas de germinación. Las semillas almacenadas a temperatura ambiente no mostraron cambios en su capacidad germinativa ni en la velocidad de germinación hasta seis meses desde cosechadas. The primary objective of this study was to establish the effects of chilling pretreatment and duration on both total germination and germination rate for seeds of Eucryphia glutinosa (Poepp. et Endl.) Baillon. Seeds were kept at 3-4°C on dry and wet sand (dry chilling and stratification, respectively) for 30, 60 and 90 days. A control (no chilling) was evaluated at 30 day intervals simultaneously with pretreated seeds. Germination was carried out in a chamber at 23°C. Average overall germination percentage was 77.0 ±6.8 %. Germination capacity of seeds was affected neither by pretreatment nor duration. Treatments had an effect on germination rate, with seeds stratificated for 60 and 90 days showing significantly higher rates of germination. Seeds stored for six months since harvest at room temperature did not show changes in germination capacity and rate of germination.
CARACTERIZACION ESPACIO-TEMPORAL DEL NICHO TROFICO DE LA FAUNA ICTICA ANDINA DEL RíO MAULE, CHILE
Acua,Patricio; Vila,Irma; Pardo,Rodrigo; Comte,Sheila;
Gayana (Concepción) , 2005, DOI: 10.4067/S0717-65382005000100022
Abstract: fluvial systems of central chile show seasonal variations in: caudal, temperature and conductivity. this has influenced the ichthic native fauna, which has been characterized as primitive, with a low number of genera and species. the coexistence on native and exotic fishes in the maule river, chile (35°43°s;71°05°w), is registered since the beginning of the xx century; system in which the ecological interactions between these species have not been adequately evaluated. for this, in between two different seasons of the year 1988, four different locations were selected in the andean zone of the maule river and its tributaries. the ichthic fauna was obtained using total census, with electrofishing equipment, stomach contents of 142 specimens of oncorhynchus mykiss, basilichthys australis and salmo trutta were analized. the principal diet of the fishes analysed was basically benthic organisms, where chironomidae, ephemoroptera and trichoptera were the predominant. horn°s index was used to compare trophic niche overlap among species, finding a high overlap index in all seasons and locations. niche overlap and benthic diversity showed a negative relationship, this may indicate changes in the intensity of ecological interactions caused by changes in the gradient of resources
CARACTERIZACION ESPACIO-TEMPORAL DEL NICHO TROFICO DE LA FAUNA ICTICA ANDINA DEL RíO MAULE, CHILE TROPHIC NICHE SPATIO-TEMPORAL CHARACTERIZATION OF THE ANDEAN ICHTHIC FAUNA AT THE MAULE RIVER, CHILE
Patricio Acua,Irma Vila,Rodrigo Pardo,Sheila Comte
Gayana (Concepción) , 2005,
Abstract: Los sistemas fluviales de la zona central de Chile presentan variaciones estacionales en: caudal, temperatura y conductividad. Esto ha influenciado a la fauna íctica nativa la que ha sido caracterizada como primitiva, representada por un bajo número de géneros y especies. La coexistencia de especies nativas e introducidas en el río Maule, Chile (35°43°S;71°05°W), se remonta a comienzos del siglo XX, sistema en el cual las interacciones ecológicas entre estas especies no han sido suficientemente evaluadas. Para ello, se seleccionaron cuatro estaciones de trabajo en el sector andino del río Maule y sus tributarios, en dos periodos del a o 1988. La fauna íctica se obtuvo realizando censo total, con un equipo de pesca eléctrica, analizándose el contenido estomacal de 142 ejemplares de Oncorhynchus mykiss, Basilichthys australis y Salmo trutta. La dieta de la mayoría de los peces analizados estuvo compuesta de organismos bentónicos, donde los taxa predominantes fueron Chironomidae, Ephemeroptera y Trichoptera. Se utilizó el índice de Horn para comparar la sobreposición de nicho trófico entre las diferentes especies, encontrándose un alto grado de sobreposición de nicho trófico en todas las estaciones y localidades. La sobreposición de nicho trófico y la diversidad bentónica se asociaron negativamente, lo que podría indicar cambios en la intensidad de las interacciones ecológicas causadas por cambios en el gradiente de recursos. Fluvial systems of central Chile show seasonal variations in: caudal, temperature and conductivity. This has influenced the ichthic native fauna, which has been characterized as primitive, with a low number of genera and species. The coexistence on native and exotic fishes in the Maule river, Chile (35°43°S;71°05°W), is registered since the beginning of the XX century; system in which the ecological interactions between these species have not been adequately evaluated. For this, in between two different seasons of the year 1988, four different locations were selected in the Andean zone of the Maule river and its tributaries. The ichthic fauna was obtained using total census, with electrofishing equipment, stomach contents of 142 specimens of Oncorhynchus mykiss, Basilichthys australis and Salmo trutta were analized. The principal diet of the fishes analysed was basically benthic organisms, where Chironomidae, Ephemoroptera and Trichoptera were the predominant. Horn°s index was used to compare trophic niche overlap among species, finding a high overlap index in all seasons and locations. Niche overlap and benthic diversity showed a neg
Tumor sólido pseudopapilar de páncreas, reporte de un caso
ACUA L,RENATO; SOTO L,PATRICIO; MU?OZ R,VANESSA; ACUA R,JAVIERA;
Revista chilena de pediatría , 2008, DOI: 10.4067/S0370-41062008000400009
Abstract: pancreatic tumors are extremely rare, specially in children, where the páncreas pseudo-papillary tumor corresponds to 1% of primary pancreatic tumors, characterized by its low malignant potential. we report the case of a solid páncreas pseudo-papillary tumor in a 5 year-old female patient, presenting with prolonged abdominal pain. an abdominal ultrasonography and computed tomography were performed, showing 2 tumors at the páncreas head and tail, with normal tumor markers (alpha-fetoprotein, carcinoembryonic antigen, p-hcg). the surgical treatment consisted on distal pancreatectomy with tumor enucleation at the páncreas head and tail, afterwards presenting acute pancreatitis that was medically treated. the biopsy confirmed páncreas solid pseudo-papillary tumor. in the 22 months follow-up period, the patient has remained disease-free
Tumor sólido pseudopapilar de páncreas, reporte de un caso Pancreas pseudo-papillary tumor, a case-report
RENATO ACUA L,PATRICIO SOTO L,VANESSA MU?OZ R,JAVIERA ACUA R
Revista chilena de pediatría , 2008,
Abstract: Objetivo: Las neoplasias de páncreas son muy infrecuentes, más aún en la edad pediátrica, y el tumor sólido pseudopapilar de páncreas corresponde alrededor del 1% de los tumores primarios de páncreas, caracterizándose por su bajo potencial maligno. Reportamos el caso clínico de un tumor sólido pseudopapilar de páncreas en paciente pediátrico. La paciente es una ni a de cinco a os, la cual consultó por dolor abdominal prolongado, dentro de su estudio se realizó ecogra a y escáner abdominal demostrando dos masas en cabeza y cola de páncreas, con marcadores tumorales (alfafetoproteína, antígeno carcinoembrionario, P-HCG) normales. Se realizó pancreatectomía distal y enucleación del tumor de cabeza de páncreas; en postoperatorio evolucionó con pancreatitis de manejo médico. Biopsia confirmó tumor sólido pseudopapilar de páncreas. En seguimiento de 22 meses la paciente se ha mantenido libre de enfermedad. Conclusión: Ante este raro tumor de páncreas se debe realizar un completo estudio preoperatorio y es recomendable la cirugía conservadora Pancreatic tumors are extremely rare, specially in children, where the páncreas pseudo-papillary tumor corresponds to 1% of primary pancreatic tumors, characterized by its low malignant potential. We report the case of a solid páncreas pseudo-papillary tumor in a 5 year-old female patient, presenting with prolonged abdominal pain. An abdominal ultrasonography and computed tomography were performed, showing 2 tumors at the páncreas head and tail, with normal tumor markers (alpha-fetoprotein, carcinoembryonic antigen, P-HCG). The surgical treatment consisted on distal pancreatectomy with tumor enucleation at the páncreas head and tail, afterwards presenting acute pancreatitis that was medically treated. The biopsy confirmed páncreas solid pseudo-papillary tumor. In the 22 months follow-up period, the patient has remained disease-free
Antibióticos y expresión de betalactamasas de espectro extendido (BLEE) en agentes bacterémicos
ACUA á,MIRTA; BENADOF F,DONA; RODRíGUEZ G,PILAR; HERRERA L,PATRICIO;
Revista chilena de pediatría , 2011, DOI: 10.4067/S0370-41062011000300004
Abstract: objective: to evaluate the association between escherischia coli (e. coli) and klebsiella spp bacteremia, both of which produce extended spectrum beta-lactamases (esbl), and the use of third generation cephalos-porines (tgc) during 30 days prior to the development of the infection. patients and methods: case study of all patients hospitalized between 2004 and 2007 at hospital de ni?os roberto del río, who presented e. coli y klebsiella spp bacteremia. results: 212 episodes of e. coli o klebsiella spp bacteremias were found; 205 contained all necessary data, 47 of which were cases and 158 were controls. the use of tgc showed a statistically significant correlation with the finding of esbl in those patients' bacteremias (or 2,5; ic95% 1,26-5). in addition, an independent statistically significant association was found with hospital acquired infections (or 8,2; ic95% 2,7-27,1), and the use of central venous catheter (or 38,9; ic95% 14,3-106,0) became close to statistical significance. conclusions: this study shows a statistically significant association between esbl bacteremias and use of tgc within 30 days prior to the infection, confirming other literature reports.
The genus Basilichthys (Teleostei: Atherinopsidae) revisited along its Chilean distribution range (21° to 40° S) using variation in morphologyand mtDNA El género Basilichthys (Teleostei: Atherinopsidae) analizado a lo largo de su distribución en Chile (21° a 40° S), utilizando rasgos morfológicos y variabilidad del ADN mitocondrial
DAVID VéLIZ,LAURA CATALáN,RODRIGO PARDO,PATRICIO ACUA
Revista chilena de historia natural , 2012,
Abstract: There is still doubt as to the number of species of the freshwater Chilean ichthyofauna, 64 % of which have conservation problems. One of the groups is that of the silversides of the genus Basilichthys. Three morphological species of this genus have been described in Chile with disjoint distributions: Basilichthys semotilus, B. microlepidotus and B. australis; the latter two overlap in distribution only in the Aconcagua River and are not easily distinguishable by morphological and meristic characters. In order to evaluate the efficacy of identification of these species by molecular techniques, we analyzed the sequence of 9 % of the mitochondrial DNA (Control Region and COI) of individuals from the Loa River (21°41' S) to the Valdivia River (39°50' S), adding meristic features for B. microlepidotus and B. australis in order to study population variation to clarify the taxonomy of the native species of the genus. The phylogenetic analysis showed that the individuals of Basilichthys semotilus form an haplogroup separated from the other species of the genus; however, B. australis and B. microlepidotus form a monophyletic group that shares the most common haplotypes. An analysis of meristic information showed no statistically significant differences in the number of lateral line scales or number of rays in the fins between B. microlepidotus and B. australis. These results do not support the current classification for the latter two species; there appears to be one group in the extreme north of the country (Basilichthys semotilus) and a second group in central Chile which should be called B. microlepidotus. This information will be useful to review the conservation status of the Chilean fauna. Si bien aún existen dudas sobre el número de especies descritas en el país, se reconoce que el 64 % de la ictiofauna dulceacuícola chilena se encuentra en alguna categoría de peligro de conservación. Uno de los grupos categorizados como vulnerable y en peligro de extinción es el de los pejerreyes del género Basilichthys. A lo largo Chile, este género posee tres especies morfológicas con distribución disjunta: Basilichthys semotilus, B. microlepidotus y B. australis. Las dos últimas sobreponen su distribución en el río Aconcagua y no son fácilmente diferenciables morfológicamente. Para evaluar la eficacia en la identificación de estas especies al utilizar marcadores moleculares, se analizó el 9 % del ADN mitocondrial (Región Control y COI) de organismos obtenidos desde el río Loa (21°41' S) al río Valdivia (39°50' S) y adicionando un análisis merístico en organismos pert
Antibióticos y expresión de betalactamasas de espectro extendido (BLEE) en agentes bacterémicos Use of antibiotics and expression of extended spectrum betalactamases (ESBL) in bacteremic agents
MIRTA ACUA á,DONA BENADOF F,PILAR RODRíGUEZ G,PATRICIO HERRERA L
Revista chilena de pediatría , 2011,
Abstract: Objetivo: Estudiar la asociación entre bacteriemia por E. coli y Klebsiella spp productoras de beta-lactamasas de espectro extendido (BLEE) y el uso de cefalosporinas de tercera generación (CTG) durante los 30 días previos al desarrollo de la infección. Método: Estudio caso control encestado desarrollado entre los a os 2004 y 2007 en el Hospital de Ni os Roberto del Río, con análisis retrospectivo de los pacientes con bacteriemias por E. coli y Klebsiella spp. Resultados: Se registraron 212 episodios de bacteriemias por E. coli o Klebsiella spp, en 205 de ellos se contó con los registros necesarios, 47 fueron casos y 158 controles. El uso de CTG se asoció de modo estadísticamente significativo con la probabilidad de aislar cepas BLEE de bacteriemias en estos pacientes (OR 2,5; IC95% 1,26-5). Además, se observó asociación independiente y estadísticamente significativa con Infección Intrahospitalaria (OR 8,2; IC95% 2,7-27,1), y el uso de Catéter Venoso Central (OR 38,9; IC95% 14,3-106,0) se acercó a la significancia estadística. Conclusiones: Nuestro trabajo establece la asociación estadística entre bacteriemia por cepas BLEE y el uso de CTG dentro de los 30 días previos al desarrollo de la infección, confirmando lo sugerido por la literatura. Objective: To evaluate the association between Escherischia Coli (E. Coli) and Klebsiella spp bacteremia, both of which produce Extended Spectrum beta-lactamases (ESBL), and the use of third generation cephalos-porines (TGC) during 30 days prior to the development of the infection. Patients and Methods: Case study of all patients hospitalized between 2004 and 2007 at Hospital de Ni os Roberto del Río, who presented E. coli y Klebsiella spp bacteremia. Results: 212 episodes of E. coli o Klebsiella spp bacteremias were found; 205 contained all necessary data, 47 of which were cases and 158 were controls. The use of TGC showed a statistically significant correlation with the finding of ESBL in those patients' bacteremias (OR 2,5; IC95% 1,26-5). In addition, an independent statistically significant association was found with hospital acquired infections (OR 8,2; IC95% 2,7-27,1), and the use of Central Venous Catheter (OR 38,9; IC95% 14,3-106,0) became close to statistical significance. Conclusions: This study shows a statistically significant association between ESBL bacteremias and use of TGC within 30 days prior to the infection, confirming other literature reports.
The genus Basilichthys (Teleostei: Atherinopsidae) revisited along its Chilean distribution range (21° to 40° S) using variation in morphologyand mtDNA
VéLIZ,DAVID; CATALáN,LAURA; PARDO,RODRIGO; ACUA,PATRICIO; DíAZ,ANGIE; POULIN,ELIE; VILA,IRMA;
Revista chilena de historia natural , 2012, DOI: 10.4067/S0716-078X2012000100004
Abstract: there is still doubt as to the number of species of the freshwater chilean ichthyofauna, 64 % of which have conservation problems. one of the groups is that of the silversides of the genus basilichthys. three morphological species of this genus have been described in chile with disjoint distributions: basilichthys semotilus, b. microlepidotus and b. australis; the latter two overlap in distribution only in the aconcagua river and are not easily distinguishable by morphological and meristic characters. in order to evaluate the efficacy of identification of these species by molecular techniques, we analyzed the sequence of 9 % of the mitochondrial dna (control region and coi) of individuals from the loa river (21°41' s) to the valdivia river (39°50' s), adding meristic features for b. microlepidotus and b. australis in order to study population variation to clarify the taxonomy of the native species of the genus. the phylogenetic analysis showed that the individuals of basilichthys semotilus form an haplogroup separated from the other species of the genus; however, b. australis and b. microlepidotus form a monophyletic group that shares the most common haplotypes. an analysis of meristic information showed no statistically significant differences in the number of lateral line scales or number of rays in the fins between b. microlepidotus and b. australis. these results do not support the current classification for the latter two species; there appears to be one group in the extreme north of the country (basilichthys semotilus) and a second group in central chile which should be called b. microlepidotus. this information will be useful to review the conservation status of the chilean fauna.
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