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Search Results: 1 - 10 of 44102 matches for " PAN Ying-Jie "
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Construction of urban modern fishery in Shanghai
上海都市型现代渔业的构建

YANG Zheng-Yong,PAN Ying-Jie,
杨正勇
,潘迎捷

中国生态农业学报 , 2008,
Abstract: 都市型现代渔业是上海都市农业的重要组成部分,是上海生态可持续发展的基础,其发展对上海食品和水源安全乃至城市安全都具有重要影响。本文在对都市型现代渔业进行界定的基础上,讨论了都市型现代渔业的特征、外延、内涵,分析了它在上海都市农业发展中的地位和作用,提出上海应通过经济产业板块、生态旅游板块、渔业知识经济板块的建设来构建其都市型现代渔业体系。其中,经济产业板块包括绿色常规水产品生产业、特种水产品生产业、远洋渔业、精深水产品加工业、现代水产品物流业等从池塘到餐桌的产业;生态旅游板块包括水体生态产业、旅游体闲渔业;渔业知识经济板块包括种源渔业和渔业信息及科技服务产业。
Entanglement Sudden Death in Band Gaps
Ying-Jie Zhang
Physics , 2009, DOI: 10.1140/epjd/e2010-00087-6
Abstract: Using the pseudomode method, we evaluate exactly time-dependent entanglement for two independent qubits, each coupled to a non-Markovian structured environment. Our results suggest a possible way to control entanglement sudden death by modifying the qubit-pseudomode detuning and the spectrum of the reservoirs. Particularly, in environments structured by a model of a density-of-states gap which has two poles, entanglement trapping and prevention of entanglement sudden death occur in the weak-coupling regime.
An efficient method for DNA extraction from compost
堆肥中微生物总DNA的高效提取

HE Li-hong,ZHAO Yong,CHEN Ming-jie,PAN Ying-jie,
何丽鸿
,赵勇,陈明杰,潘迎捷

微生物学报 , 2006,
Abstract: An efficient method for obtaining DNA from compost which contained high levels of organic matter was developed. The protocol consisted of washing with phosphate-EDTA before extraction, cell lysis with hot-SDS and enzymes (lysozyme, lywalzyme, proteinase K), removing humic acid and other inhibitors with PVPP and precipitation with PEG-8000. The compost total DNA was extracted from four different composts, the DNA yield was 63.54 +/- 12.08 to approximately 106.50 +/- 28.36 microg/g of dry compost. Molecular size of DNA obtained using this protocol was about 23kb and contained low protein and humic acid contamination with the A260/A280 ratios exceeding 1.6 and A260/A230 ratios reaching 1.8. Usually, additional purification steps such as agarose gel electrophoresis, gel permeation chromatography, or affinity chromatography were needed to get PCR-amplifiable DNA, but the DNA obtained using this protocol could directly be used to PCR-amplification and restriction enzyme digestion. Just like purity of DNA template, lower DNA yield also appears to introduce a bias towards lower community diversity. In this study compared the purified DNA the direct DNA reveals higher microbial community diversity assessed by denaturing gradient gel electrophoresis(DGGE) of amplified V3 region of 16S rDNA.
Distribution of a specific SCAR marker among Lentinula edodes protoplast monokaryons for strain 135
香菇135菌株特异SCAR标记在其原生质体单核中的分布

ZHANG Mei-Yan,TAN Qi,CHEN Ming-Jie,PAN Ying-Jie,
张美彦
,谭琦,陈明杰,潘迎捷

微生物学报 , 2008,
Abstract: Refrigerated mycelium and fresh fruitbody tissue-isolated mycelium of Lentinula edodes strain 135 were used to prepare protoplast monokaryons. Fifty-eight and 83 monokaryons were isolated respectively and their mating types were determined. PCR amplification employing specific primers was used to establish the distribution among the protoplast monokaryons of a SCAR marker specific for strain 135. Protoplast monokaryons segregated into either A1B1 or A2B2 mating type, and the SCAR marker was detected only in the latter. The outcome is a regular distribution pattern that demonstrates the stable inheritance of the SCAR marker and is fundamental to identification of offspring arising from crosses between protoplast monokaryons of this particular mating type and other karyons.
SELECTION FOR COLD-RESISTENT STRAIN BY COMPLEX MUTAGENESIS OF VOLVARIELLA VOLVACEA AND IDENTIFICATION OF THE STRAIN
草菇耐低温菌株的诱变选育与鉴定

HAN Ye-Jun,CHEN Ming-Jie CAO Hui PAN Ying-Jie,
韩业君
,曹晖,陈明杰,潘迎捷

菌物学报 , 2004,
Abstract: 在草菇V23原生质体形成和再生的最佳条件下制备原生质体。分别用紫外线(UV),60Co-γ射线,硫酸二乙酯(DES)对原生质体进行复合诱变。初筛、复筛获得耐常规低温(4℃)10d的菌株VH。栽培实验证明:VH在26℃下的生物学效率明显优于V23(CK),且差异极显著。同工酶分析表明:VH过氧化物同工酶,酯酶同工酶谱带数量及酶活性均发生变化;RAPD实验显示:VH相对于V23的变异系数为0.213。VH菇体正常,味道鲜美,食用后无毒副作用。氨基酸组成分析表明:除总氨基酸和谷氨酸含量VH高于V23外,其它氨基酸差异不明显。VH遗传性状稳定,是具有潜在应用价值的优良菌株。
DETECTING IN 9 EXTRACELLULAR ENZYME ACTIVITIES OF AGROCYBE AEGERITA STRAINS
柱状田头菇生长发育中9种胞外酶活性的测定

WANG Nan,SHEN Feng,TAN Qi,CHEN Ming-Jie,PAN Ying-Jie,
王南
,沈锋,谭琦,陈明杰,潘迎捷

菌物学报 , 2000,
Abstract: 柱状田头菇Agrocybe aegerita作为一种新开发的食用菌,越来越受到消费者的喜爱和研究者的关注,但在其栽培生产中遇到的产量相对较低的问题,一直未能得到很好的解决,致使柱状田头菇的栽培难以得到大规模的推广。本研究对不同来源的柱状田头菇在其6个不同生长发育阶段中,9种与培养料中主要组分分解相关的胞外酶活性的变化进行了测定,结果表明:柱状田头菇属褐腐菌,对非木质纤维素的利用能力最强,对纤维素的利用能力较强,对木质素的利用能力较差,但柱状田头菇具有漆酶活性。
Cloning and sequence analysis of cold induced genes in Chinese straw mushroom, Volvariella volvacea
草菇冷诱导相关基因的克隆及序列分析

SUN Xiao-Hong,FENG Ai-Ping,CHEN Ming-Jie,PAN Ying-Jie,
孙晓红
,冯爱萍,陈明杰,潘迎捷

菌物学报 , 2006,
Abstract: 利用差异显示技术分离获得草菇低温特异DNA片段,经与正常草菇和低温诱导草菇cDNA分别southern杂交验证后,得到低温特异性片段。采用PCR标记技术对获得的低温特异性片段进行DIG标记,以此为探针,对低温处理的草菇cDNA文库进行筛选,获得4个阳性克隆,分别进行测序。序列同源性比较分析发现,Cor3基因与s-腺苷-L-高半胱氨酸水解酶有很高的同源性,Cor4基因与40S核糖体蛋白S9有很高的同源性,这两个基因可能与草菇的低温自溶现象有关。Cor1基因与脉孢菌的保守假设蛋白(conservedhypotheticalprotein)有同源性,Cor2基因与辅酶A连接酶有同源性。半定量RT-PCR验证发现Cor1和Cor2基因在正常情况下没有表达,低温处理后有表达,Cor3和Cor4基因在正常情况下有表达,低温处理后表达量增加。
Distribution of a specific SCAR marker among Lentinula edodes protoplast monokaryons for strain 135
香菇135菌株特异SCAR标记在其原生质体单核中的分布

ZHANG Mei-Yan,TAN Qi,CHEN Ming-Jie,PAN Ying-Jie,
张美彦
,谭琦,陈明杰,潘迎捷

菌物学报 , 2008,
Abstract: Refrigerated mycelium and fresh fruitbody tissue-isolated mycelium of Lentinula edodes strain 135 were used to prepare protoplast monokaryons. Fifty-eight and 83 monokaryons were isolated respectively and their mating types were determined. PCR amplification employing specific primers was used to establish the distribution among the protoplast monokaryons of a SCAR marker specific for strain 135. Protoplast monokaryons segregated into either A1B1 or A2B2 mating type, and the SCAR marker was detected only in the latter. The outcome is a regular distribution pattern that demonstrates the stable inheritance of the SCAR marker and is fundamental to identification of offspring arising from crosses between protoplast monokaryons of this particular mating type and other karyons.
CD200-CD200R dysfunction exacerbates microglial activation and dopaminergic neurodegeneration in a rat model of Parkinson's disease
Shi Zhang, Xi-Jin Wang, Li-Peng Tian, Jing Pan, Guo-Qiang Lu, Ying-Jie Zhang, Jian-Qing Ding, Sheng-Di Chen
Journal of Neuroinflammation , 2011, DOI: 10.1186/1742-2094-8-154
Abstract: 6-hydroxydopamine (6-OHDA)-lesioned rats were used as an animal model of PD. CD200R-blocking antibody (BAb) was injected into striatum to block the engagement of CD200 and CD200R. The animals were divided into three groups, which were treated with 6-OHDA/Veh (PBS), 6-OHDA/CAb (isotype control antibody) or 6-OHDA/BAb, respectively. Rotational tests and immunohistochemistry were employed to evaluate motor deficits and dopaminergic neurodegeneration in animals from each group. HPLC analysis was used to measure monoamine levels in striatum. Morphological analysis and quantification of CD11b- (or MHC II-) immunoreactive cells were performed to investigate microglial activation and possible neuroinflammation in the substantia nigra (SN). Finally, ELISA was employed to assay protein levels of proinflammatory cytokines.Compared with 6-OHDA/CAb or 6-OHDA/Veh groups, rats treated with 6-OHDA/BAb showed a significant increase in counts of contralateral rotation and a significant decrease in TH-immunoreactive (TH-ir) neurons in SN. A marked decrease in monoamine levels was also detected in 6-OHDA/BAb-treated rats, in comparison to 6-OHDA/Veh-treated ones. Furthermore, remarkably increased activation of microglia as well as up-regulation of proinflammatory cytokines was found concomitant with dopaminergic neurodegeneration in 6-OHDA/BAb-treated rats.This study shows that deficits in the CD200-CD200R system exacerbate microglial activation and dopaminergic neurodegeneration in a 6-OHDA-induced rat model of PD. Our results suggest that dysfunction of CD200-CD200R signalling may be involved in the aetiopathogenesis of PD.Parkinson's disease (PD) is the second most common neurodegenerative disease in the world, and is characterized by dopaminergic neuron loss in the substantia nigra pars compacta (SNpc) [1]. PD was first described by James Parkinson in 1817, and the aetiology of PD still remains unknown. However, emerging investigations suggest that multiple factors, both genetic an
Optimum Isolation and Structural Analysis of Polysaccharide from the Fruiting Bodies of Coprinus comatus
鸡腿菇子实体多糖分离纯化工艺及结构研究

YAO Yu-Jing,YANG Ren-Zhi,ZHANG Jing-Song,PAN Ying-Jie,
姚毓婧
,杨仁智,张劲松,潘迎捷

微生物学通报 , 2007,
Abstract: 以多糖得率为指标,用正交试验对鸡腿菇子实体多糖的提取纯化工艺进行优化。用离子色谱和凝胶渗透色谱对粗多糖进行分离纯化,利用化学和光谱学方法对均一多糖CC30w-1进行结构分析。结果表明鸡腿菇子实体多糖最佳提取工艺为:提取次数为3次,提取时间为1.5h,提取温度为95℃,料液比为1∶12;最佳脱蛋白条件:样品-氯仿 正丁醇为3∶1(V/V),氯仿-正丁醇为3∶1(V/V),反应时间为20min,脱蛋白次数为7;结构分析的结果表明:CC30w-1分子量为1.94×104Da,糖组成为Fuc∶Gal=1∶4.02;岩藻糖以端基方式连接,半乳糖主要以1,6-和1,2,6-两种方式连接,3个主要的连接方式的摩尔比为1.15∶2.88∶1;主要由(1,6)-α-D-Galp糖残基构成主链,在O-2位被α-Fucp糖残基取代的5个单糖残基组成的结构重复单元。
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