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Search Results: 1 - 10 of 200621 matches for " P. Theerakulpisut "
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Two New Records of Coleochaetalean Algae (Coleochaetales, Chlorophyta) from Northeast Thailand
W. Mahakham,P. Theerakulpisut
International Journal of Botany , 2010,
Abstract: This study is an attempt to contribute and add information on the freshwater algal floral in Thailand. In this study, two members of coleochaetalean green algae, viz., Chaetosphaeridium globosum (Norstedt) Klebahn and Coleochaete nitellarum Jost are reported for the first time for Thailand. Both of them were found as epiphytes on charophyte algae in a mesotrophic reservoir, namely Hui Nam Sab from Amnat Charoen province, Northeast Thailand. Illustrated descriptions of the newly recorded species along with a short note on the species and its distribution are provided. In addition, some ecological aspects of the studied reservoir and coleochaetalean algae are discussed.
Exogenous Sorbitol and Trehalose Mitigated Salt Stress Damage in Salt-sensitive but not Salt-tolerant Rice Seedlings
P. Theerakulpisut,W. Gunnula
Asian Journal of Crop Science , 2012,
Abstract: Salinity is one of the major factors limiting plant growth by imposing the osmotic, ion toxicity and oxidative stresses. The accumulation of osmoprotectants is a mechanism in which plants employ to protect tissue damages caused by salinity stress. The present study aimed to investigate the effect of two osmoprotectants (Sorbitol: Sor and Trehalose: Tre) on growth and physiology of rice (Oryza sativa L.) cvs. Khao Dawk Mali (KDML105; salt-sensitive) and Pokkali (PK: salt-tolerant) under NaCl stress. Under salt stress, KDML105 showed a significantly reduced growth and a large increase in the amount of hydrogen peroxide (H2O2), lipid peroxidation as indicated by Malondialdehyde (MDA) content and membrane Electrolyte Leakage (EL). The effects of salt were less severe in PK. Exogenously supplied Sor and Tre were able to enhance growth of salt-stressed KDML105 and alleviated the negative effects of salt by reducing H2O2 and MDA content. However, Sor and Tre did not have any beneficial effect on growth of PK plants. In some cases Sor worsened the salt-induced effects on lipid peroxidation and membrane damage. It can be concluded that exogenous Sor and Tre showed protective roles for salt-sensitive KDML105 but not salt-tolerant PK and the effects were more pronounced for Tre than Sor.
The Effect of Plant Growth Regulators and Organic Supplements on Callus Induction and Plant Regeneration in Rice (Oryza sativa L.)
K. Rattana,P. Theerakulpisut,S. Bunnag
Asian Journal of Plant Sciences , 2012,
Abstract: Abiotic and biotic stresses are the limiting factors of growth and productivity of rice in many agriculture areas of Thailand. Plant Tissue cultures have been used for breeding purpose, especially in selection for stress tolerance. The aim of this study was to investigate and find out enhancement on callus induction and regeneration in rice cultivars; Khao Dawk Mali 105, Supanburi 1, Chai Nat 1 and Pathum Thani 1. The suitable media for callus induction of KDML 105, Supanburi 1, Chai Nat 1 and Pathum Thani 1 were NN medium supplemented with 2, 1.5, 2.5 and 1 mg L-1 2,4-D, respectively. It was found that addition of 300 mg L-1 casein hydrolysate enhanced callus formation in KDML 105. Combination with 300 mg L-1 casein hydrolysate and 1,000 mg L-1 L-proline can influence for callus induction in Supanburi 1. Addition of 500 mg L-1 L-proline can enhance callus induction of Chai Nat 1 and Pathum Thani 1. The MS medium supplemented with 3 mg L-1 BA and 300 mg L-1 casein hydrolysate was suitable for regeneration of KDML 105. The most suitable medium for regeneration in Supanburi 1 was MS medium combination with 3 mg L-1 BA, 0.5 mg L-1 NAA and 500 mg L-1 L-proline. Addition of 5 mg L-1 BA, 0.5 mg L-1 NAA and combination with 300 mg L-1 casein hydrolysate in MS medium were the optimal for regeneration in Chai Nat 1. Combination with 5 mg L-1 BA and 300 mg L-1 casein hydrolysate can influence for regeneration in Pathum Thani 1.
Pretreatment with Spermidine Reverses Inhibitory Effects of Salt Stress in Two Rice (Oryza sativa L.) Cultivars Differing in Salinity Tolerance
P. Saleethong,J. Sanitchon,K. Kong-ngern,P. Theerakulpisut
Asian Journal of Plant Sciences , 2011,
Abstract: Environmental stresses including soil salinity are severely affecting rice growth and productivity. The triamine Spermidine (Spd), a member of Polyamines (PAs), a group of phytohormone-like natural amine compounds has been shown to play essential roles in salt stress tolerance in many important crop plants. The effects of exogenously supplied Spd were investigated in two rice cultivars differing in salt tolerance level to determine the roles of Spd on the modulation of physiological parameters related to salt-stress responses. Thirty day-old seedlings of two rice cultivars, Pokkali (salt-tolerant) and KDML 105 (salt-sensitive), grown in nutrient solution were exposed or not to 1 mM Spd for 24 h before submitted to salinized solution containing 150 mM NaCl for 7 days. Salinity stress resulted in significant reduction in plant height, fresh and dry weight of both cultivars but Spd pretreatment significantly increased these growth parameters only in Pokkali. Salt-stress induced considerable disturbance in several physiological processes inhibitory for growth including chlorophyll loss, accumulation of hydrogen peroxide and increase in lipid peroxidation, increased electrolyte leakage and increase in Na+/decrease in K+/Na+. Spd pretreatment led to the reversal of those inhibitory effects in both cultivars. This study showed that exogenous Spd can be applied as short-term pretreatment prior to introduction of salt stress to help elevate salt tolerance of rice and confirmed earlier observations that exogenous Spd offered protective roles on salinity-stressed rice by stabilizing membrane, scavenging free radicals and maintaining K+/Na+ status.
Pollen morphology of the genus Cornukaempferia (Zingiberaceae) in Thailand
植物分类学报 , 2009,
Abstract: Cornukaempferia is a recently described genus of Zingiberaceae which only occurs in Thailand as a rare genus with limited geographical distribution. Only three species have been described so far, including a recently described new species, C. larsenii. These three species are morphologically very similar and additional data on other biological aspects are useful for the elucidation of their relationship. Pollen morphology of all three species of genus Cornukaempferia has been studied by light microscopy and scanning electron microscopy. The pollen grains are monad, spherical, inaperturate. The exine sculpture is echinate with psilate between the spines for C. aurantiflora and C. longipetiolata, or echinate with regulate between the spines for C. larsenii. This observation helps support the taxonomic status of C. larsenii.
Screening Some Tomato Commercial Cultivars from Thailand for Salinity Tolerance
Sumalee Chookhampaeng,Wattana Pattanagul,Piyada Theerakulpisut
Asian Journal of Plant Sciences , 2007,
Abstract: Hydroponically-grown seedlings of thirteen commercial tomato cultivars and breeding lines were exposed to 0 (control) and 200 mM NaCl (salt stress) for ten days. Salt tolerance was evaluated based on visual appearance of plant damage and the plants were assigned the scale from 1 (most tolerant) to 4 (most sensitive). The salinity scales ranged from 1.00-3.75. All genotypes responded to salt by an accumulation of Na+, reduction in K+, Ca2+, N and shoot/root dried weight and a small increase in P. Salinity tolerance scales, ion concentration and shoot/root dried weight differed greatly among tomato genotypes. Highest correlation was found between salinity tolerance scale classes and the reduction in root dried weight, followed by the reduction in shoot dried weight, Na+ concentration, Ca2+ /Na+ and K+/Na+ ratios. The amount of N and P content did not correlate with salinity scale classes. In general, Na+ concentration and the reduction in root/shoot dried weight were the most reliable parameters useful for screening salt tolerance of tomato at the vegetative stage.
Agrobacterium-Mediated Transformation of Dendrobium secundum (Bl.) Lindl with Antisense ACC Oxidase
Porntip Atichart,Sumontip Bunnag,Piyada Theerakulpisut
Asian Journal of Plant Sciences , 2007,
Abstract: The suitable medium which resulted in 98% for seed germination was the Vacin and Went (1949) medium supplemented with 100 mg L-1 myo-inositol, 1 mg L-1 thiamine, 1 mg L-1 nicotinic acid, 1 mg L-1 pyridoxine and 4 mg L-1 glycine, 15% coconut water and 0.8% agar at pH 5.4 under light condition. Protocorms with high proliferation capacity were cultured on the modified VW medium and 0.5 mg L-1 NAA. Genetics transformation of Dendrobium secundum mediated by Agrobacterium tumefaciens strain LBA 4404, which harbored the plasmid pCAMBIA 1305.1 containing antisense ACC oxidase, β-glucuronidase (GUS) and hygromycin resistance (hptII) genes. The efficiency of transformation was 40 min co-cultivation of protocorms with A. tumefaciens that has been induced with 200 μΜ acetosyringone. An experiment was conducted to determine the effect of antibiotic on protocorm growth. It was found that hygromycin at 25 mg L-1 was effective for transformant selection. The maximum concentration of cefotaxime that protocorm could tolerate was 500 mg L-1. Hygromycin resistant protocorms showed histochemical blue staining due to GUS activity was 60%. Successful the transformation was confirmed by PCR analysis. It was found that the sizes of amplified fragments were 195, 180 and 843 bp for the 35S, NOS and antisense ACC oxidase, respectively.
Genetic diversity analysis of rice cultivars (Oryza sativa L.) differing in salinity tolerance based on RAPD and SSR markers
Kanawapee,Nantawan; Sanitchon,Jirawat; Srihaban,Pranee; Theerakulpisut,Piyada;
Electronic Journal of Biotechnology , 2011,
Abstract: thirty rice cultivars were evaluated for salinity tolerance during the seedling stage and were divided into five tolerance groups including tolerant (t), moderately tolerant (mt), moderately susceptible (ms), susceptible (s) and highly susceptible (hs) which comprised 5, 10, 9, 4 and 2 cultivars respectively. genetic diversity of all rice cultivars was evaluated using random amplified polymorphic dna (rapd) and simple sequence repeats (ssr) markers. the cultivars were evaluated for polymorphisms after amplification with 20 random decamer primers and 20 ssr primer pairs. a total of 161 rapd markers and 190 ssr alleles were produced which revealed 68.94% and 89.47% polymorphism respectively. mean genetic similarity coefficient was 0.82 for rapd and 0.70 for ssr. cluster analysis based on rapd markers was effective in grouping cultivars based on their salt tolerance ability. group ia1, ib and iv contained three t, three s and two hs rice cultivars respectively. the mt and ms cultivars which showed similar physiological responses to salinity were resolved into two groups: group ia2 and group ii comprising ten and eight mt/ms cultivars respectively. cluster analysis based on ssr markers separated rice cultivars into groups based on genetic relatedness which did not correspond to salinity tolerance level. the results from this study provided some useful implications for salt tolerance breeding programs. the evaluation of genetic similarity and cluster analysis together with salt tolerance ability provides some useful guides for assisting plant breeders in selecting suitable genetically diverse parents for the crossing program.
Transformation of indica rice (Oryza sativa L.) cv. RD6 mediated by Agrobacterium tumefaciens
Thodsaporn Pipatpanukul,Sumontip Bunnag,Piyada Theerakulpisut,Manit Kosittrakul
Songklanakarin Journal of Science and Technology , 2004,
Abstract: High percentage of callus induction at 97% was obtained when seeds of rice (Oryza sativa L.) cv. RD6 were cultured on modified N6 medium supplemented with 3% (w/v) sucrose, 22.5 μM 2,4-D and 0.8% agar under light condition. The suitable regeneration medium was N6 medium supplemented with 3% (w/v) sucrose, 2.5 μM IAA, 18 μM BA and 0.8% agar. A test had been performed to determine the effect of antibiotics on the regeneration of rice cv. RD6. It was found that kanamycin concentration up to 150 mg l-1 and hygromycin concentration at 10 mg l-1 were effective for selection of transformants. Cefotaxime and carbenicillin concentration up to 250 mg l-1 had the highest phytotoxicity to plant regeneration. Agrobacterium-mediated gene transfer protocols for rice cv. RD6 were performed using A. tumefaciens strain LBA4404, which harbored the plasmid pBI121 containing genes for β- glucuronidase (GUS) and kanamycin resistance (nptII), and strain EHA105, which harbored plasmid pCAMBIA1301 containing genes for β-glucuronidase (GUS) and hygromycin resistance (hptII). GUS activities were found in rice calli after co-cultivation. A number of morphologically normal fertile transgenic rice plants were obtained. Stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transgenic plants in T0 and T1 generation. Mendelian segregation was observed in T1 progeny.
Transformation of rice (Oryza sativa L.) cv. Chainat 1 using chitinase gene
Kedsukon Maneewan,Sumontip Bunnag,Piyada Theerakulpisut,Manit Kosittrakun
Songklanakarin Journal of Science and Technology , 2005,
Abstract: As prerequisites for generating stable transformed rice (Oryza sativa L.) cv. Chainat 1, efforts were made to improve the efficiency of regeneration systems for rice cv. Chainat 1. The suitable medium which resulted in 85.7% callus induction from rice seeds was N6 medium supplemented with 4.5 μM 2,4-D, 2.5 μM NAA and 500 mg/l casein hydrolysate under light condition. The calli were dehydrated in a petridish for 5 days before being transferred to regeneration medium. The suitable medium for shoot regeneration from the calli of rice cv. Chainat 1 was MS medium supplemented with 9 μM BA, 1 μM NAA and 300 mg/l casein hydrolysate. An experiment was conducted to determine the effect of antibiotics on callus induction. It was found that hygromycin at 20 mg/l was effective for transformant selection. The highest concentration of cefotaxime that the calli could tolerate was 400 mg/l. The genetic transformation of rice cv. Chainat 1 mediated by Agrobacterium tumefaciens strain LBA4404, which harbored the plasmid pCAMBIA 1305.1 containing chitinase gene, β-glucuronidase (GUS) and hygromycin resistance (hptII), was used in the procedure. The optimal co-cultivation time was 30 minutes. Particle bombardment was also used to transform chitinase, GUS and hptII genes, using GUS assay to test transformation efficiency under the proper conditions. It was found that particle bombardment at the distance of 9 cm from stopping screen to callus brought about the highest transformation efficiency. PCR method confirmed the integration of chitinase gene, selectable marker and screenable marker in the transformants.
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