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Search Results: 1 - 10 of 200733 matches for " P Padula "
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Ecología y evolución de hantavirus en el Cono Sur de América
Murua,R; Padula,P;
Archivos de medicina veterinaria , 2004, DOI: 10.4067/S0301-732X2004000100001
Abstract: hantavirus are associated with a single primary rodent host of the familiy muridae in three sub families, two of them murinae and arvicolinae distributed in the paleartic region (europa, asia, china) and the sub family sigmodontinae in north, central and south america besides an arvicolinae genus (microtus) in north america. studies on the host and virus phylogeny show close similarities when are compared which implies that hantavirus are very ancient infectious agents which have coevolved with the rodent host. the history of earth, tectonic movements and climatic changes which affected the continents in the past are relevant to understand the host reservoir and its micro parasites current geographic distribution. this review provides historical biogeography of the sigmodontine rodents, phylogentic analyses of hantavirus, its molecular epidemiology and its geographical distribution in south america in order to sustain the proposal that the virus- rodent interaction has coevolved in the neartic before the family muridae was detached in subfamilies and before the sigmodontines rodents spread into the south american continent. it is described the existence of a large number of hantavirus lineages with small differences which make difficult to establish so far, well define species of hantavirus. an analisis between similarities and differences in the ecology and pathogenesis of two virus which have produced an important number of human cases in north america (sin nombre) and in the southern cone of america (virus andes) is discussed.
Ecología y evolución de hantavirus en el Cono Sur de América Ecology and evolution of hantavirus in the Southern Cone of America
R Murua,P Padula
Archivos de medicina veterinaria , 2004,
Abstract: Los hantavirus tienen huéspedes especie específicos pertenecientes a una familia común Muridae con tres sub familias, dos de ellas Murinae y Arvicolinae que se distribuyen en áreas geográficas de Europa Asia y Oceanía con un género Arvicolinae en América del Norte y la Sub familia Sigmodontinae en Centro América y Sudamérica. Estudios de la filogenia del huésped y el virus muestran fuertes similitudes al ser comparados, lo que sugiere una asociación de mucha más larga data con un proceso de coevolución entre el agente infeccioso y sus huéspedes roedores. La historia de la tierra y los procesos tectónicos y climáticos que afectaron al continente en épocas pretéritas son relevantes para comprender la actual distribución de los reservorios huéspedes y sus parásitos. Se entregan antecedentes biogeográficos de los roedores con la distribución geográfica de los hantavirus en Sudamérica, análisis filogenético de los virus, epidemiología molecular que sustentan la propuesta que el virus y el roedor han coevolucionado antes del momento de separarse la Familia Muridae en subfamilias (Murinae, Arvicolinae y Sigmodontinae) y anterior al ingreso de los roedores sigmodontinos al continente sudamericano. Se discute la dificultad en demarcar especie nueva de hantavirus y la existencia de varios linajes con diferencias peque as entre si para ser consideradas como especies virales. Se describen diferencias y similitudes entre las dos especies de hantavirus que más casos han producido en América del Norte (virus Sin Nombre) y en el Cono Sur de de América (Virus Andes). Hantavirus are associated with a single primary rodent host of the familiy Muridae in three sub families, two of them Murinae and Arvicolinae distributed in the Paleartic Region (Europa, Asia, China) and the sub family Sigmodontinae in North, Central and South America besides an Arvicolinae genus (Microtus) in North America. Studies on the host and virus phylogeny show close similarities when are compared which implies that hantavirus are very ancient infectious agents which have coevolved with the rodent host. The history of earth, tectonic movements and climatic changes which affected the continents in the past are relevant to understand the host reservoir and its micro parasites current geographic distribution. This review provides historical biogeography of the sigmodontine rodents, phylogentic analyses of hantavirus, its molecular epidemiology and its geographical distribution in South America in order to sustain the proposal that the virus- rodent interaction has coevolved in the Neartic before the Fa
Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surface
D Docheva,D Padula,C Popov,P Weishaupt
European Cells and Materials (ECM) , 2010,
Abstract: Periodontal ligament (PDL) can be obtained from patients undergoing orthodontic treatment. PDL contains progenitor cells that can be expanded and differentiated towards several mesenchymal lineages in vitro. Furthermore, PDL-derived cells have been shown to generate bone- and PDL-like structures in vivo. Thus, PDL cells, combined with suitable biomaterials, represent a promising tool for periodontitis-related research and PDL engineering.Here, a new PDL cell line using lentiviral gene transfer of human telomerase reverse transcriptase (hTERT) was created. HTERT-expressing PDL cells showed similar morphology and population doubling time but an extended lifespan compared to the primary cells. In addition, PDL-hTERT cells expressed several characteristic genes and upon osteogenic stimulation produced a calcified matrix in vitro. When cultivated on two topographically different titanium scaffolds (MA and SLA), PDL-hTERT cells exhibited augmented spreading, survival and differentiation on smooth (MA) compared to rough (SLA) surfaces. These findings differ from previously reported osteoblast behaviour, but they are in agreement with the behaviour of chondrocytes and gingival fibroblasts, suggesting a very cell type-specific response to different surface textures.In summary, we report the testing of titanium biomaterials using a new PDL-hTERT cell line and propose this cell line as a useful model system for periodontitis research and development of novel strategies for PDL engineering.
Deep Imaging: How Much of the Proteome Does Current Top-Down Technology Already Resolve?
Elise P. Wright, Kali A. G. Prasad, Matthew P. Padula, Jens R. Coorssen
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0086058
Abstract: Effective proteome analyses are based on interplay between resolution and detection. It had been claimed that resolution was the main factor limiting the use of two-dimensional gel electrophoresis. Improved protein detection now indicates that this is unlikely to be the case. Using a highly refined protocol, the rat brain proteome was extracted, resolved, and detected. In order to overcome the stain saturation threshold, high abundance protein species were excised from the gel following standard imaging. Gels were then imaged again using longer exposure times, enabling detection of lower abundance, less intensely stained protein species. This resulted in a significant enhancement in the detection of resolved proteins, and a slightly modified digestion protocol enabled effective identification by standard mass spectrometric methods. The data indicate that the resolution required for comprehensive proteome analyses is already available, can assess multiple samples in parallel, and preserve critical information concerning post-translational modifications. Further optimization of staining and detection methods promises additional improvements to this economical, widely accessible and effective top-down approach to proteome analysis.
Nueva evidencia epidemiológica y molecular a favor de la transmisión interhumana para el linaje Sout del hantavirus Andes
Pinna,Diego M.; Martinez,Valeria P.; Bellomo,Carla M.; López,Claudia; Padula,Paula;
Medicina (Buenos Aires) , 2004,
Abstract: after the hantavirus pulmonary syndrome (hps) outbreak occurred in southern argentina (patagonia) in 1996, a new mechanism of hantavirus spread was described for andes virus (and): person-to-person transmission. since then, this mode of transmission has been limited to the endemic area of and sout lineage. we report here an hps case, patient a, with residence in buenos aires province, whose unique epidemiological risk factor was to have shared a trip to southern argentina with an already ill person, later confirmed as an hps case (patient i). a third case (patient b), with residence in buenos aires province, developed hps after several contact events with patient a, with no additional risk factors. hantavirus infection was confirmed in all three patients by serological diagnosis and viral genetic characterization. the viral lineage characterized was and sout, being the same strain in the three cases. the clinical picture of patient a showed profound compromise, with gastrointestinal symptoms, with no clinical evidence of pulmonary involvement. in this work we present new evidence supporting person-to-person transmission for and sout lineage, where there was an only contact with the index case, occasional, prolonged and non-repeated, established out of the endemic area for and sout lineage.
A Therapeutic Potential for Marine Skeletal Proteins in Bone Regeneration
David W. Green,Matthew P. Padula,Jerran Santos,Joshua Chou,Bruce Milthorpe,Besim Ben-Nissan
Marine Drugs , 2013, DOI: 10.3390/md11041203
Abstract: A vital ingredient for engineering bone tissue, in the culture dish, is the use of recombinant matrix and growth proteins to help accelerate the growth of cultivated tissues into clinically acceptable quantities. The skeletal organic matrices of calcifying marine invertebrates are an untouched potential source of such growth inducing proteins. They have the advantage of being ready-made and retain the native state of the original protein. Striking evidence shows that skeleton building bone morphogenic protein-2/4 (BMP) and transforming growth factor beta (TGF-β) exist within various marine invertebrates such as, corals. Best practice mariculture and the latest innovations in long-term marine invertebrate cell cultivation can be implemented to ensure that these proteins are produced sustainably and supplied continuously. This also guarantees that coral reef habitats are not damaged during the collection of specimens. Potential proteins for bone repair, either extracted from the skeleton or derived from cultivated tissues, can be identified, evaluated and retrieved using chromatography, cell assays and proteomic methods. Due to the current evidence for bone matrix protein analogues in marine invertebrates, together with the methods established for their production and retrieval there is a genuine prospect that they can be used to regenerate living bone for potential clinical use.
Clinimetric properties of the Brazilian-Portuguese version of the Quick Exposure Check (QEC) Propriedades clinimétricas da vers o para o português-brasileiro do "Quick Exposure Check" (QEC)
Maria L. C. Comper,Leonardo O. P. Costa,Rosimeire S. Padula
Brazilian Journal of Physical Therapy , 2012, DOI: 10.1590/s1413-35552012005000049
Abstract: BACKGROUND: Most of the available instruments aimed to assess risk exposure associated with work-related musculoskeletal disorders were originally developed in English, which makes their use difficult in countries such as Brazil. OBJECTIVE: To test the clinimetric properties of the Quick Exposure Check (QEC) instrument previously adapted into Brazilian-Portuguese. METHOD: The original version of the QEC was translated and cross-culturally adapted into Brazilian-Portuguese and tested with 107 workers in two sectors of a textile factory. The internal consistency and construct validity were tested using only baseline values from the participants while reproducibility (reliability and agreement) was evaluated in a test-retest design with a seven-day interval. RESULTS: The adapted version presented appropriate levels of internal consistency (Cronbach's α=0.76); moderate intra-observer reliability (ICCs ranging from 0.41 to 0.60); moderate to substantial inter-observer reliability (ICCs ranging between 0.62 and 0.86). The standard error of the measurement (SEM) ranged from 8.3 to 11.2 points. Moderate levels of construct validity (Pearson's r=0.38) were observed. CONCLUSIONS: The Brazilian version of the QEC has appropriate clinimetric properties for measuring different levels of exposure to ergonomics risk factors and can now be used by Brazilian researchers and Occupational Health professionals. CONTEXTUALIZA O: A maior parte dos instrumentos utilizados para avalia o dos fatores de risco de desenvolvimento dos distúrbios musculoesqueléticos relacionados ao trabalho foram originalmente desenvolvidos em língua inglesa, dificultando seu uso em países cuja língua primária n o é o inglês, como é o caso do Brasil. OBJECTIVO: Testar as propriedades clinimétricas do instrumento Quick Exposure Check (QEC) para o português-brasileiro. MéTODO: A vers o original do QEC foi traduzida e adaptada transculturalmente para o português-brasileiro e testada em 107 trabalhadores de dois setores de produ o de uma indústria têxtil. A consistência interna e a validade de construto foram testadas utilizando apenas valores basais dos participantes, enquanto a reprodutibilidade foi avaliada em um delineamento de teste e reteste, com intervalo de sete dias. RESULTADOS: A vers o adaptada apresentou níveis adequados de consistência interna (α Cronbach=0,76), confiabilidade intraobservador moderada (ICC entre 0,41 e 0,60) e confiabilidade interobservador variando de moderada a substancial (ICC entre 0,62 e 0,86). O erro-padr o da medida (EPM) variou de 8,3 a 11,2 pontos. Níveis modera
Clinimetric properties of the Brazilian-Portuguese version of the Quick Exposure Check (QEC) Propriedades clinimétricas da vers o para o português-brasileiro do "Quick Exposure Check" (QEC)
Maria L. C. Comper,Leonardo O. P. Costa,Rosimeire S. Padula
Brazilian Journal of Physical Therapy , 2012,
Abstract: BACKGROUND: Most of the available instruments aimed to assess risk exposure associated with work-related musculoskeletal disorders were originally developed in English, which makes their use difficult in countries such as Brazil. OBJECTIVE: To test the clinimetric properties of the Quick Exposure Check (QEC) instrument previously adapted into Brazilian-Portuguese. METHOD: The original version of the QEC was translated and cross-culturally adapted into Brazilian-Portuguese and tested with 107 workers in two sectors of a textile factory. The internal consistency and construct validity were tested using only baseline values from the participants while reproducibility (reliability and agreement) was evaluated in a test-retest design with a seven-day interval. RESULTS: The adapted version presented appropriate levels of internal consistency (Cronbach's α=0.76); moderate intra-observer reliability (ICCs ranging from 0.41 to 0.60); moderate to substantial inter-observer reliability (ICCs ranging between 0.62 and 0.86). The standard error of the measurement (SEM) ranged from 8.3 to 11.2 points. Moderate levels of construct validity (Pearson's r=0.38) were observed. CONCLUSIONS: The Brazilian version of the QEC has appropriate clinimetric properties for measuring different levels of exposure to ergonomics risk factors and can now be used by Brazilian researchers and Occupational Health professionals. CONTEXTUALIZA O: A maior parte dos instrumentos utilizados para avalia o dos fatores de risco de desenvolvimento dos distúrbios musculoesqueléticos relacionados ao trabalho foram originalmente desenvolvidos em língua inglesa, dificultando seu uso em países cuja língua primária n o é o inglês, como é o caso do Brasil. OBJECTIVO: Testar as propriedades clinimétricas do instrumento Quick Exposure Check (QEC) para o português-brasileiro. MéTODO: A vers o original do QEC foi traduzida e adaptada transculturalmente para o português-brasileiro e testada em 107 trabalhadores de dois setores de produ o de uma indústria têxtil. A consistência interna e a validade de construto foram testadas utilizando apenas valores basais dos participantes, enquanto a reprodutibilidade foi avaliada em um delineamento de teste e reteste, com intervalo de sete dias. RESULTADOS: A vers o adaptada apresentou níveis adequados de consistência interna (α Cronbach=0,76), confiabilidade intraobservador moderada (ICC entre 0,41 e 0,60) e confiabilidade interobservador variando de moderada a substancial (ICC entre 0,62 e 0,86). O erro-padr o da medida (EPM) variou de 8,3 a 11,2 pontos. Níveis modera
Open innovation as an alternative for strategic development in the aerospace industry in Brazil
Mariana de Freitas Dewes,Odair Lelis Gon?alez,Angelo Pássaro,Antonio Domingos Padula
Journal of Aerospace Technology and Management , 2010,
Abstract: We present in this paper a case of technological competence development in the aerospace sector in Brazil, by addressing the complete cycle of integrated circuits for satellite applications, an area of high technology which is strategic to the country. The development of technological and business competences is linked to an understanding of the existing relations between different participating institutions, both public and private. There is an effort to establish a network for the development of radiation-hard integrated circuits in Brazil, comprising universities, research centers, private companies, design houses, funding and governmental agencies. These institutions have been working to define their roles, through participation in federally funded projects to develop robust component technology for the aerospace industry in Brazil. As a means to maintain and improve this network, it is suggested that long term planning tools such as technology roadmaps be adopted, as well as measures to increase awareness of and help clarify intellectual property issues, which is considered a significant bottleneck to advance technology development in this area. In this sense, open innovation may be considered an alternative for competitively enhancing the outcomes of the sector.
Back-to-Back Correlations for Finite Expanding Fireballs
Sandra S. Padula,G. Krein,T. Csorgo,Y. Hama,P. K. Panda
Physics , 2005, DOI: 10.1103/PhysRevC.73.044906
Abstract: Back-to-Back Correlations of particle-antiparticle pairs are related to the in-medium mass-modification and squeezing of the quanta involved. They are predicted to appear when hot and dense hadronic matter is formed in high energy nucleus-nucleus collisions. The survival and magnitude of the Back-to-Back Correlations of boson-antiboson pairs generated by in-medium mass modifications are studied here in the case of a thermalized, finite-sized, spherically symmetric expanding medium. We show that the BBC signal indeed survives the finite-time emission, as well as the expansion and flow effects, with sufficient intensity to be observed at RHIC.
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