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Search Results: 1 - 10 of 214999 matches for " Otto; Pérez Guevara "
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Evaluación de un sistema de Western Blot (DAVIH-BLOT) para la confirmación de anticuerpos al VIH-1
OTTO CRUZ SUI,MARíA TERESA PéREZ GUEVARA,MARICELA IZQUIERDO MáRQUEZ,LEONOR LOBAINA BATELEMY
Revista Cubana de Medicina Tropical , 1997,
Abstract: Se reporta la evaluación del sistema DAVIH-BLOT (Laboratorios DAVIH, Cuba) frente a los paneles de proficiencia enviados al Laboratorio Nacional de Referencia del SIDA, por la Organización Mundial de la Salud (OMS), para el aseguramiento de la calidad en el diagnóstico de anticuerpos al VIH en los a os 1989, 1991 y 1993, así como la comparación del sistema con los de las firmas DuPont y Diagnostic Pasteur en un grupo de 467 sueros positivos por ELISA. En el estudio de las muestras de la OMS el sistema demostró ser altamente sensible y específico. Al compararlos con los estuches HIV-1 Western blot IgG versión 1.2 (DuPont Company, EE.UU.) y New LAV-blot 1 (Diagnostic Pasteur, Francia) tuvo una coincidencia del 100 y 97 %, respectivamente. It is reported the evaluation of the DAVIH-BLOT system (DAVIH Laboratories, Cuba) against the proficiency panels sent to the National AIDS Reference Laboratory by the World Health Organization (WHO) in order to ensure the quality in the diagnosis of HIV antibodies during 1989, 1991 and 1993. The system is also compared with those of the Dupont and Diagnostic Pasteur firms in a group of 467 positive sera by ELISA. On studying the WHO's samples, the system proved to be highly sensitive and specific. In the comparison made with the HIV-1 Western Blot IgG version 1.2 (Dupont company, USA) and with the LAV-blot 1 (Diagnostic Pasteur, France), there was a coincidence of 100 and 97 %, respectively.
Fluido oral y orina como muestras alternativas en el diagnóstico confirmatorio de la infección por VIH-1
Cruz Sui,Otto; Pérez Guevara,. María Teresa; Lubián Caballero,Ana Luisa; Montano Tamayo,Lucy; Silva Cabrera,Eladio;
Revista Cubana de Medicina Tropical , 2007,
Abstract: the objective of this report was to evaluate oral fluid and urine as optional samples for hiv-1 confirmatory test using davih-blot system (laboratorios davih, la habana, cuba), for which they were compared with their corresponding serum samples in a group of 125 individuals. in band pattern analysis, predominant antibodies in positives oral fluid and urine samples against p34, p68, gp41, gp120, were no different from their corresponding sera according to the proportion comparison test (p<0,001). relative sensitivity and specificity of this system were 100 % in oral fluid and 98, 75% and 100% in urine samples respectively. these results support optional use of oral fluid and urine that, with slight modifications in the diagnostic system, can be applied for hiv-1 antibody confirmation testing.
Fluido oral y orina como muestras alternativas en el diagnóstico confirmatorio de la infección por VIH-1 Oral fluid and urine as alternative samples in confirmatory testing of HIV-1 infection
Otto Cruz Sui,. María Teresa Pérez Guevara,Ana Luisa Lubián Caballero,Lucy Montano Tamayo
Revista Cubana de Medicina Tropical , 2007,
Abstract: Se evaluó el fluido oral y la orina como muestras opcionales en el diagnóstico confirmatorio del VIH-1 con el sistema DAVIH-BLOT (Laboratorios DAVIH, La Habana, Cuba), para lo cual se comparó con las correspondientes muestras de sueros en un grupo de 125 individuos. En el análisis de los patrones de bandas reveladas, los anticuerpos predominantes en las muestras positivas de fluido oral y orina contra la p34, p68, gp41 y gp120, no tuvieron diferencias con sus correspondientes sueros según la prueba de comparación de proporciones (p< 0,001). La sensibilidad y especificidad relativas del sistema resultaron de 100 % en el análisis del fluido oral; 98,75 y 100 %, respectivamente, en las muestras de orina. Estos resultados apoyan el empleo opcional del fluido oral y la orina que, con ligeras modificaciones introducidas en el sistema diagnóstico, pueden aplicarse para la confirmación de anticuerpos contra el VIH-1. The objective of this report was to evaluate oral fluid and urine as optional samples for HIV-1 confirmatory test using DAVIH-BLOT system (Laboratorios DAVIH, La Habana, Cuba), for which they were compared with their corresponding serum samples in a group of 125 individuals. In band pattern analysis, predominant antibodies in positives oral fluid and urine samples against p34, p68, gp41, gp120, were no different from their corresponding sera according to the proportion comparison test (p<0,001). Relative sensitivity and specificity of this system were 100 % in oral fluid and 98, 75% and 100% in urine samples respectively. These results support optional use of oral fluid and urine that, with slight modifications in the diagnostic system, can be applied for HIV-1 antibody confirmation testing.
Determinación de anticuerpos al virus de la inmunodeficiencia humana tipo 1 (VIH-1) en muestras de sangre seca en papel de filtro
María Teresa Pérez Guevara,Felipe M. Rolo Gómez,Carmen Nibot Sánchez,Otto Cruz Sui
Revista Cubana de Medicina Tropical , 1998,
Abstract: Se estudia la posibilidad de detectar anticuerpos al VIH-1 mediante un estuche de Immunoblotting, en un papel de 125 muestras conocidas de sangre seca colectadas en papel de filtro y su correspondiente muestra de suero. No se apreciaron diferencias en los patrones de bandas con ambos tipos de muestras, así como en la sensibilidad y especificidad, donde se alcanzaron cifras del 100 %, lo que permitió concluir que la muestra de sangre tomada en papel de filtro puede ser empleada para la detección de anticuerpos al VIH-1 por el sistema DAVIH-BLOT y puede ser conservada a 4 oC por 30 semanas. The possibility of detecting HIV-1 antibodies by an inmunoblotting kit is studied in a panel of 125 known specimens of dried blood spotted on filter paper and their corresponding serum samples. No differences were observed in the patterns of bands with both types of samples or in the sensitivity and specificity, where 100 % figures were attained, allowing to conclude that the blood specimen taken on filter paper may be used for the detection of HIV-1 antibodies by the DAVIH-BLOT system and may be kept at 4 oC during 30 weeks.
Determinación de anticuerpos al virus de la inmunodeficiencia humana tipo 1 (VIH-1) en muestras de sangre seca en papel de filtro
Pérez Guevara,María Teresa; Rolo Gómez,Felipe M.; Nibot Sánchez,Carmen; Cruz Sui,Otto; Rodríguez,Orfelina;
Revista Cubana de Medicina Tropical , 1998,
Abstract: the possibility of detecting hiv-1 antibodies by an inmunoblotting kit is studied in a panel of 125 known specimens of dried blood spotted on filter paper and their corresponding serum samples. no differences were observed in the patterns of bands with both types of samples or in the sensitivity and specificity, where 100 % figures were attained, allowing to conclude that the blood specimen taken on filter paper may be used for the detection of hiv-1 antibodies by the davih-blot system and may be kept at 4 oc during 30 weeks.
Pesquisaje de anticuerpos contra el virus linfotrópico de células T humanas tipo I (HTLV-I) en donantes de sangre y grupos de riesgo
SILVA CABRERA,ELADIO; PéREZ GUEVARA,MARíA T.; LUBIáN CABALLERO,ANA L.; DE LA FUENTE ARZOLA,JOSé L.; NAVEA LEYVA,LEONOR; CRUZ SUI,OTTO;
Revista Cubana de Medicina Tropical , 1997,
Abstract: it was studied the presence of htlv-i antibodies in 3 774 sera, 1 409 of them were from blood donors, 1 444 were from patients who had suffered from some sexually transmitted disease recently, and 921 from transfused patients. the davih htlv i (elisa) and davih blot htlv i (western blot) systems produced at the davih laboratories (havana, cuba) were used for screening and confirmation, respectively. of the 68 reactive sera utilized in the elisa test, it was confirmed the presence of htlv i/ii antibodies in 2, whereas 12 were considered as undetermined by the western blot.
Evaluación de un sistema de Western Blot (DAVIH-BLOT) para la confirmación de anticuerpos al VIH-1
CRUZ SUI,OTTO; PéREZ GUEVARA,MARíA TERESA; IZQUIERDO MáRQUEZ,MARICELA; LOBAINA BATELEMY,LEONOR; RUIBAL BRUNET,IGNACIO; SILVA CABRERA,ELADIO;
Revista Cubana de Medicina Tropical , 1997,
Abstract: it is reported the evaluation of the davih-blot system (davih laboratories, cuba) against the proficiency panels sent to the national aids reference laboratory by the world health organization (who) in order to ensure the quality in the diagnosis of hiv antibodies during 1989, 1991 and 1993. the system is also compared with those of the dupont and diagnostic pasteur firms in a group of 467 positive sera by elisa. on studying the who's samples, the system proved to be highly sensitive and specific. in the comparison made with the hiv-1 western blot igg version 1.2 (dupont company, usa) and with the lav-blot 1 (diagnostic pasteur, france), there was a coincidence of 100 and 97 %, respectively.
Seroprevalencia de la infección por HTLV-1 en diferentes grupos de riesgo estudiados en Cuba
Lubián Caballero,Ana Luisa; Díaz Torres,Héctor Manuel; Silva Cabrera,Eladio; Pérez Guevara,María Teresa; Cruz Sui,Otto; de la Fuente Arzola,José Luis; Navea Leyva,Leonor; Noa Romero,Enrique;
Revista Cubana de Medicina , 1998,
Abstract: in the period between 1991 and 1996, 26 352 serum samples from different risk groups and blood donors, were studied to know the seroprevalence of antibodies against the human t-lymphotropic virus type i (htlv-i), and also as a continuation of researches carried out between 1989 and 1990. the elisa davih-htlv-i system was used for the antibodies investigation, and as a certifying test, the western blot davih-blot htlv-i was used too, both from the cuban davih laboratories. the presence of anti htlv-i antibodies was confirmed in 10 persons, and in most of them the epidemiologic study could elucidate the probable way of contagion. the seropositivity index observed was of 0,037 %, and is low if compared with the seroprevalence rates reported for the caribbean area.
Temporal variation of biomass and productivity of Thalassia testudinum (Hydrocharitaceae) in Venezuela, Southern Caribbean
Pérez,Daisy; Guevara,Marcel; Bone,David;
Revista de Biología Tropical , 2006,
Abstract: annual biomass and productivity of thalassia testudinum were determined during a year at a seagrass bed located in the parque nacional morrocoy, venezuela. leaf, rhizome and root biomass were determined monthly, together with short-shoot density, from february 1992 to january 1993, from nine replicated core samples. productivity was measured using the methodology by zieman (1974) with minor modifications, and leaf turnover rate was calculated. leaf biomass values ranged between 101.73 dry g m-2 in february and 178.11 dry g m-2 in august. productivity ranged from 1.69 dry g m-2 d-1 in april and october to 3.30 dry g m-2 d-1 in july, showing two annual peaks: one in july and one in march. the leaf turnover rate showed the highest value in june (2.41% d-1) and the lowest in may (1.23% d-1). sampling time differences in leaf biomass, productivity and turnover rate were statistically significant. short- shoot density values varied between 811.10 shoots m-2 in april and 1226.08 shoots m-2 in december, but the differences were not significant along the year. these results indicated seasonal trends for leaf biomass, productivity and turnover rate of t. testudinum in the southern caribbean (latitude 10?n). rev. biol. trop. 54(2): 329-339. epub 2006 jun 01.
Temporal variation of biomass and productivity of Thalassia testudinum (Hydrocharitaceae) in Venezuela, Southern Caribbean
Daisy Pérez,Marcel Guevara,David Bone
Revista de Biología Tropical , 2006,
Abstract: Annual biomass and productivity of Thalassia testudinum were determined during a year at a seagrass bed located in the Parque Nacional Morrocoy, Venezuela. Leaf, rhizome and root biomass were determined monthly, together with short-shoot density, from February 1992 to January 1993, from nine replicated core samples. Productivity was measured using the methodology by Zieman (1974) with minor modifications, and leaf turnover rate was calculated. Leaf biomass values ranged between 101.73 dry g m-2 in February and 178.11 dry g m-2 in August. Productivity ranged from 1.69 dry g m-2 d-1 in April and October to 3.30 dry g m-2 d-1 in July, showing two annual peaks: one in July and one in March. The leaf turnover rate showed the highest value in June (2.41% d-1) and the lowest in May (1.23% d-1). Sampling time differences in leaf biomass, productivity and turnover rate were statistically significant. Short- shoot density values varied between 811.10 shoots m-2 in April and 1226.08 shoots m-2 in December, but the differences were not significant along the year. These results indicated seasonal trends for leaf biomass, productivity and turnover rate of T. testudinum in the Southern Caribbean (latitude 10 N). Rev. Biol. Trop. 54(2): 329-339. Epub 2006 Jun 01. Durante un a o se determinaron mensualmente la productividad foliar, la densidad de tallos cortos y la biomasa de hojas, tallos cortos, rizomas y raíces de Thalassia testudinum, en una "pradera" localizada en el Parque Nacional Morrocoy, Venezuela. Los valores de biomasa foliar estuvieron entre 101.73 g/m2 en febrero y 178.11 g/m2 en agosto, los de productividad foliar se ubicaron entre 1.69 g/m2/d en abril y octubre y 3.30 g/m2/d en julio, mostrando dos picos anuales, uno en julio y otro en marzo. La tasa de recambio foliar mostró el mayor valor en junio (2.41%/d) y el menor en mayo (1.23%/d). Tales diferencias fueron estadísticamente significativas durante el a o para todas estas variables. La densidad de tallos cortos fluctuó entre 811.10 tallos/m2 en abril y 1 226.08 tallos/m2 en diciembre, pero las diferencias no fueron significativas a lo largo del a o. Estos resultados indicaron una tendencia estacional para la biomasa foliar, la productividad foliar y la tasa de recambio de las hojas de T. testudinum en el Caribe Sur, a 10o N de latitud.
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